• Research in Plant Disease
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• v.20 no.3
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• pp.173-181
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• 2014

The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-to-country transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

• Journal of Korea Port Economic Association
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• v.18 no.1
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• pp.43-60
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• 2002

Apart from the DEA method, the traditional measuring systems of container terminal productivities generally bring to light the partial productive efficiencies, because they evaluate only the productivity of each individual facility. However, these methods do not play roles of the reasonable productivity measures to compare and evaluate the efficiencies of terminals to meet the requirement of terminal customers like as shipping lines, shippers, etc. with the cutthroat competition in container handling market. As the suppliers of service, the terminals struggle to cope with the needs of buyers' markets due to the lack of suitable criteria to measure the efficiencies. Therefore, this paper uses the simulation approach to reveal which strategies enhance the productivities through the container terminal analysis. An additional intent of this paper is to build the active strategies to present what the experimental indexes like as norm-time exactness ratio, norm-time excess ratio, etc. compare between terminals to measure the total productivities for the age of global supply chain management. However, the experimental productivity indexes presented in this paper require a validation of the exact usefulness, by consideration from all angles in the future, and a development to more reasonable measuring method/indexes for the container terminal productivities.

• Korean Management Science Review
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• v.10 no.1
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• pp.139-156
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• 1993

Various ATM switches have been proposed since Asynchronous Transfer Mode (ATM) was recognized as appropriate for implementing broadband integrated services digital network (BISDN). An ATM switching network may be evaluated on two sides : traffic performances (maximum throughput, delay, and packet loss probability, etc.) and structural features (complexity, i.e. the number of switch elements necessary to construct the same size switching network, maintenance, modularity, and fault tolerance, etc.). ATM switching networks proposed to date tend to show the contrary characteristics between structural features and traffic performance. The Knockout Switch, which is well known as one of ATM switches, shows a good traffic performance but it needs so many switch elements and buffers. In this paper, we propose an input and output buffered Knockout Switch for the purpose of reducing the number of switch elements and buffers of the existing Knockout Switch. We analyze the traffic performance and the structural features of the proposed switching architecture through a discrete time Markov chain and compare with those of the existing Knockout Switch. It was found that the proposed architecture could reduce more than 40 percent of switch elements and more than 30 percent of buffers under a given requirement of cell loss probability of the switch.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.11
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• pp.843-851
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• 2020

This study focuses on identifying and prioritizing the broader performance measures for the service supply chains by taking the case of Majan Electricity Company, Sohar, in the Sultanate of Oman. For an examination of the uniformity of ultimate objectives and the priorities therein, two strata of respondents with a total of fourteen respondents were approached for their valuable insights. Suitable structured instruments were personally administered to elicit the insightful and worthy responses. The two multi-criteria decision-making techniques, namely, the Fuzzy Analytical Hierarchy Process and the Best-Worst Method were used to reach a meaningful prioritization of the identified and refined broader performance measurement dimensions. The results show that there exists a minor gap between the two respondents' groups in terms of their prioritizations. The major finding points to the difference in terms of the topmost priorities as revealed by the two set of respondents. For one group of respondents, the customer satisfaction matters the most, whereas for the other group, it is the overall profitability that matters the most. This gap against the utopian state assists in concluding that there is a requirement to reorient the employees so as to have a shared and common understanding of the organizational priorities.

• The Korean Journal of Zoology
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• v.22 no.4
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• pp.141-152
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• 1979

Feline leukemia virus DNA polymerase was purified by ion-exchange and nucleic acid affinity chromatographies. The enzyme consists of a single polypeptide chain of approximately 72, 000 molecular weight as determined by both of a glycerol density gradient centrifugation and SDS-polyacrylamide gel electrophoresis. The preferred divalent cation for DNA synthesis is $Mn^2+$ on a variety of template-primers, and its optimum concentration appears to be significantly lower than reported results of other mammalian type-C viral enzymes. The divalent cation requirement for maximum activity of RNase H is similar to those of DNA polymerase. Both DNA polymerase and RNase H activities appear to reside on the same molecule as demonstrated by the copurification of both activities through various purification steps. An additional RNase H without detectible polymerase activity was generated by a limited chymotrypsin digestion. This RNase H activity was inhibited equally effectively as RNase H in the intact reverse transcriptase by antisera prepared against reverse transcriptase of feline leukemia virus. Neutralization and binding test showed that antibody binding to reverse transcriptase molecule did not completely inhibit the polymerase activity.

• Journal of Communications and Networks
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• v.18 no.2
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• pp.162-172
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• 2016

The placement of the complete baseband processing in a centralized pool results in high data rate requirement and inflexibility of the fronthaul network, which challenges the energy and cost effectiveness of the cloud radio access network (C-RAN). Recently, redesign of the C-RAN through functional split in the baseband processing chain has been proposed to overcome these challenges. This paper evaluates, by mathematical and simulation methods, different splits with respect to network level energy and cost efficiency having in the mind the expected quality of service. The proposed mathematical model quantifies the multiplexing gains and the trade-offs between centralization and decentralization concerning the cost of the pool, fronthaul network capacity and resource utilization. The event-based simulation captures the influence of the traffic load dynamics and traffic type variation on designing an efficient fronthaul network. Based on the obtained results, we derive a principle for fronthaul dimensioning based on the traffic profile. This principle allows for efficient radio access network with respect to multiplexing gains while achieving the expected users' quality of service.

• Journal of Biosystems Engineering
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• v.25 no.4
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• pp.257-264
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• 2000

A tractor attached TMR(model 430) mixer has been developed in a previous study. However, the mixer was found to be improved through field applications in its capacity, manufacturing cost, ergonomic design and power-train requirement. The TMR mixer was modified into a model TMR500, approved by Institute of Agricultural Mechanization, as follows : 1. Roughage cutting system was seperated from the mixer, resulting in the 33% reduction of manufacturing cost. 2. Enlarged hopper capacity enabled to feed 60 heads at a batch. 3. Hydraulically controlled gate system saved ergonomic man power. 4. Power transmission system was changed from a chain-sprocket system(27:1) to the gear-train reduction system(38.6:1) to satisfy the recommended use of 540rpm PTO input.

• Journal of the Korea Society of Computer and Information
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• v.14 no.7
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• pp.1-8
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• 2009

In this paper, make a study decision problem called an optimal release policies after testing a software system in development phase and transfer it to the user. The optimal software release policies which minimize a total average software cost of development and maintenance under the constraint of satisfying a software reliability requirement is generally accepted. The Bayesian parametric inference of model using log Poisson execution time employ tool of Markov chain(Gibbs sampling and Metropolis algorithm). In a numerical example by T1 data was illustrated. make out estimating software optimal release time from the maximum likelihood estimation and Bayesian parametric estimation.

• The Korean Journal of Food And Nutrition
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• v.17 no.3
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• pp.254-259
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• 2004

Rapid detection of foodbome pathogens is becoming increasingly important. The requirement for faster, more reliable tests has lead to the development of a wide range of rapid methods. Among these methods, the use of systems based on nucleic acid based detection has been increasing since they offer advantages of reduction in test time and more reliable detection or identification. Random Amplification Polymorphic DNA(RAPD) method has been used to fingerprint foodbome microorganisms; Listeria monocytogenes. In this study, 10-mer primer OPG-13(5'-CTCTCCGCCA-3') was used to generate RAPD-PCR for detection of pathogenic L. monocytogenes of Listeria spp. Among 20 primers tested, OPG-13 showed on acceptable result for the differentiation of a pathogenic Listeria from non-pathogenic microorganisms. Pathogenic Listeria, L. monocytogenes(ATCC 15313, 19111, 19112, 19113) showed two bands for 700 bp and 1,500 bp while non-pathogenic bacteria, L. ivanovii, L. grayi, L. murrayi, L. innocua, L. welshimeri, and L. seeligeri had only one band sizing from 2,000 to 2,300 bp. This RAPD method proved to be a valuable to gain important information on sources of pathogenic bacteria in food industry.

• Journal of the Korean Chemical Society
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• v.5 no.1
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• pp.33-37
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• 1961

We have shown that carboxy-peptidase destroys the biological activity of angiotensin octa-and deca-peptides. Since Proline occurs as the seventh amino acid from the amino end of the chain and since carboxypeptidase does not cleave proline from a peptid chain, it is evident that the heptapeptid H.asp-arg-val-tyr-ileu-his-pro.OH is formed by this hydrolysis. This peptide must then be biologically inactive. In order to determine whether the phenyl group of the C-terminal amino acid was the necessary requirement for biological activity of the octapeptide, $ala^8$ angiotensin octapeptide(amino acids of peptides numbered from amino end) was synthesized. For this synthesis the four dipeptides were prepared: carbobenzoxy-L-prolyl-L-alanine-P-nitrobenzyl-ester, m.p. $134-135^{\circ}C,$ carbobenzoxy-L-isoleucyl-imidazole benzyl-L-histidine methyl ester, m.p. $114-116^{\circ}C,$ carbobenzoxy-L-valyl-L-tyrosine hydrazide and carbobenzoxy B-benzyl-L-aspartyl-nitro-L-arginine. The first three dipeptides were obtained as crystalline compounds. Imidazole-benzyl-L-histidine was used in the hope that it would block the histidine imidazole against side reactions in steps subsequent to the formation of the C-terminal tetrapeptide. Also, it was through that the imidazole benzylated peptides would be easier to crystallize. This, however, was not the case. The tetrapeptide, carbobenzoxy-L-isoleucyl-L-im, benzyl-histidyl, L-prolyl-L-alanine-nitrobenzyl ester was not obtained in a crystalline form. Neither could the mono-or dihydrobromide of the tetrapeptide free base be induced to crystallize. Carbobenzoxy-L-valyl-L-tyrosine azide was condensed with the tetrapeptide free base to yield the protected hexapeptide; carbobenzoxy-L-valyl-L-tyrosyl-L-isoleucyl-L-im, benzyl, histidyl-L-Prolyl-L-alanine-nitrobenzyl ester. Upon removal of the carbobenzoxy group with hydrogen bromide in acetic acid an amorphous free base hexapeptide ester was obtained. This compound gave the correct C, H, N analysis and contained the six amino acids in the correct ratio. The octapeptide was obtained by condensing this hexapeptide with carbobenzoxy-B-benzyl-L-aspartyl-nitro, L-arginine using the mixed anhydride method of condensation. This amorphous product was proven to be homogenous by chromatography in two solvent systems and upon hydrolysis yielded the eight amino acids in correct ratio. The five protecting groups were removed from the octapeptide by hydrogenolysis over palladium black catalyst. Biological assay of the free peptide indicated that it possessed less than 0.1 per cent of both pressor and oxytocic activity of the phenylalanine8 angiotensin. This suggests that the phenyl group is a point of attachment between angiotensin and its biological receptor site.