• Asian-Australasian Journal of Animal Sciences
• /
• 제29권4호
• /
• pp.487-499
• /
• 2016

The aim of the present study was to examine the effects of testosterone (T) and estradiol-$17{\beta}$ ($E_2$) on the production of progesterone ($P_4$) by granulosa cells, and of the $E_2$ on the production of $P_4$ and T by theca internal cells. In the first experiment, granulosa cells isolated from the largest ($F_1$) and third largest ($F_3$) preovulatory follicle were incubated for 4 h in short-term culture system, $P_4$ production by granulosa cells of both $F_1$ and $F_3$ was increased in a dose-dependent manner by ovine luteinizing hormone (oLH), but not T or $E_2$. In the second experiment, $F_1$ and $F_3$ granulosa cells cultured for 48 h in the developed monolayer culture system were recultured for an additional 48 h with increasing doses of various physiological active substances existing in the ovary, including T and $E_2$. Basal $P_4$ production for 48 h during 48 to 96 h of the cultured was about nine fold greater by $F_1$ granulosa cells than by $F_3$ granulosa cells. In substances examined oLH, chicken vasoactive intestinal polypeptide (cVIP) and T, but not $E_2$, stimulated in a dose-dependent manner $P_4$ production in both $F_1$ and $F_3$ granulosa cells. In addition, when the time course of $P_4$ production by $F_1$ granulosa cells in response to oLH, cVIP, T and $E_2$ was examined for 48 h during 48 to 96 h of culture, although $E_2$ had no effect on $P_4$ production by granulosa cells of $F_1$ during the period from 48 to 96 h of culture, $P_4$ production with oLH was found to be increased at 4 h of the culture, with a maximal 9.14 fold level at 6 h. By contrast, $P_4$ production with cVIP and T increased significantly (p<0.05) from 8 and 12 h of the culture, respectively, with maximal 6.50 fold response at 12 h and 6, 48 fold responses at 36 h. Furthermore, when $F_1$ granulosa cells were precultured with $E_2$ for various times before 4 h culture with oLH at 96 h of culture, the increase in $P_4$ production in response to oLH with a dose-related manner was only found at a pretreatment time of more than 12 h. In the third experiment, theca internal cells of $F_1$, $F_2$ and the largest third to fifth preovulatory follicles ($F_{3-5}$) were incubated for 4 h in short-term culture system with increasing doses of $E_2$. The production of $P_4$ and T by theca internal cells were increased with the addition of $E_2$ of $10^{-6}M$. These increases were greater in smaller follicles. These results indicate that, in granulosa cells of the hen, T may have a direct stimulatory action in the long term on $P_4$ production, and on $E_2$ in long-term action which may enhance the sensitivity to LH for $P_4$ production, and thus, in theca internal cells, $E_2$ in short term action may stimulate the production of $P_4$ and T.

• 한국농림기상학회지
• /
• 제2권1호
• /
• pp.1-8
• /
• 2000

본 연구는 변색립, 수발아를 유발시킨 기상환경을 구명하고 출수기의 변색립, 등숙 중 .후기의 수발아, 생식생장기의 침관수, 황숙기의 도복에 타른 수량반응을 구명하기 위하여 피해를 받은 농가 포장 및 경기도농업기술원 포장에서 조사한 결과는 다음과 같다. 벼 출수시기에 영화변색 발생시의 기상조건은 평균 기온은 21.3-26.4$^{\circ}C$, 상대습도는 75.2-98.4%, 증발계수는 19.3, 풍속은 10.8-13.m/sec.이였으며, 영화변색비율이 63.2-65.7%, 38.3-45.2%일 때 임실비율과 등숙비율 저하로 수량이 각각 26-27%. 10-17%감소하였다. 벼 등숙 중.후기에 포장에서 연속강우에 의한 수발아 발생 기상조건은 7일간 연속강우로 상대습도가 96% 이상이였고 7일간의 평균기온은 18.9$^{\circ}C$, 최고기온은 21.9$^{\circ}C$, 최저기온은 16.8$^{\circ}C$이였으며, 수발아율이 높은 품종은 주안벼(45.5%), 진부벼(14.5%). 봉광벼(14.2%), 오봉벼(12.6%) 등 이였다. 벼 생식생장기 관수시 곁가지는 1.5-2m 수심에 1일간 관수부터 발생하였고, 3-4m 수심에 2-3일간 관수시는 m$^2$당 269-571개 발생하여 전체수량에 32-52%기여하였다. 벼 황숙기 도복시 일으켜 세움과 방치시 수량 차는 대차없었으나 방치시 미숙립 증가로 완전미비율이 세움에 비하여 중묘기계이앙답은 9.1%, 건답직파답은 12.5% 낮아졌다.

• 한국환경농학회지
• /
• 제20권4호
• /
• pp.211-217
• /
• 2001

본 연구는 경작지 중금속 오염지역의 식물복원을 위한 방안으로, Zn, Cu처리가 담배식물의 생리반응에 영향하는 형태적인 변화와 엽령에 따른 중금속 축적 양상 및 무기이온의 흡수관계를 분석한 결과는 다음과 같다. Zn, Cu에 따른 간장의 생장감소는 1.0 mM이하에서는 작았고, 지상부 건물중보다는 지하부의 건물중을 크게 감소시켰다. 특히 지하부의 건물중은 약 50%가 감소하였다. Zn, Cu처리시 지상 ${\cdot}$ 지하부의 축적은 처리농도가 증가함에 따라 완만히 증가하다가 2.0 mM이상에서는 급격히 증가하였다. 엽령에 따라 Zn은 노화엽에서, Cu는 생장엽에서 축적량이 많았다. 주당 축적량은 2.0 mM에서 Zn은 146,000 mg/kg, Cu는 20,500 mg/kg이었고, 식물수확시기는 Zn은 영양생장기에 Cu는 생식생장기가 적합하였다. Zn, Cu처리에 따른 Mg, Mn, Fe함량은 처리농도가 증가할수록 감소하였다. 지상 ${\cdot}$ 지하부 모두 Zn처리구에서는 Cu함량은 감소하고, Cu처리구에서는 Zn함량은 증가하였다.

• 디자인학연구
• /
• 제19권5호
• /
• pp.313-322
• /
• 2006

화면을 구성하는 여러 가지 조형요소 중에서 형태는 그려지고 표현된 모양에 따라 어린이의 심리적 반응을 불러일으킬 뿐만 아니라 이야기내용에 따른 주제와 배경의 관계, 등장인물이 처한 상황이나 감정 등을 전달하는 시각적 진술방식이라고 할 수 있다. 본 연구는 화면에 표현된 형태가 시각적, 심리적으로 어린이에게 어떻게 작용 하는가를 분석하기 위하여 먼저, 형태에 대한 어린이의 지각발달과 지각경험이 어린이의 연령에 따라 다르게 나타나는 것을 살펴보았으며, 어린이의 독창성과 창의성발달, 시각적, 심리적 사고발달, 그리고 꿈과 상상력 자극에 영향을 미친다는 것을 파악하였다. 그리고 형태의 표현형식을 재현적 형태표현, 단순화된 형태표현, 과장된 형태표현, 왜곡된 형태표현으로 각각 구분하여 그 특성을 알아보고, 이를 1, 2차에 걸쳐 진행되는 설문자료로 활용하여 어린이의 표현 형태별 유형에 따른 반응과 선호도를 분석하였다. 끝으로 어린이의 반응분석 통하여 도출된 결과는 일러스트레이터의 개성과 창의성이 바탕이 된 형태표현에 도움이 됨은 물론, 일러스트레이션을 배우는 학생들의 효과적인 표현방법 연구와 실험에 활용될 것으로 사료된다.

• 한국자원식물학회지
• /
• 제19권2호
• /
• pp.227-231
• /
• 2006

본 연구는 2종류의 하우스, 즉 1-2W 형 하우스와 무기둥하우스에, 시설억제재배, 촉성계배, 반측성재배 그리고 여름재배의 4 재배시기에 8개의 오이 품종을 재식하여, 재배시기에 따른 품종들의 암수꽃의 발생, 개체 당 과실수에 따른 퇴화 과실수, 그리고 수량을 조사 비교하여 각 작형에 알맞은 품종들을 추천하기 위해 수행하였다. 시설억제재배에서는 낙합계나 흑진주계 또는 입추백다다기오이가 생육 및 수량이 양호하였고, 촉성재배에서는 겨우살이청장오이, 입추백다다기오이 그리고 가을낙합오이가 추천된다. 반측성재배에서는 청장계 오이나 낙합계 오이가 비교적 양호한 수량을 보였으며 여름재배에서는 남부청장오이나 가을낙합오이가 양호하였다.

• 한국발생생물학회지:발생과생식
• /
• 제21권2호
• /
• pp.157-165
• /
• 2017

One of the reasons to causing blood coagulation in the tissue of xenografted organs was known to incompatibility of the blood coagulation and anti-coagulation regulatory system between TG pigs and primates. Thus, overexpression of human CD73 (hCD73) in the pig endothelial cells is considered as a method to reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study was performed to produce and breed transgenic pigs expressing hCD73 for the studies immune rejection responses and could provide a successful application of xenotransplantation. The transgenic cells were constructed an hCD73 expression vector under control porcine Icam2 promoter (pIcam2-hCD73) and established donor cell lines expressing hCD73. The numbers of transferred reconstructed embryos were $127{\pm}18.9$. The pregnancy and delivery rate of surrogates were 8/18 (44%) and 3/18 (16%). The total number of delivered cloned pigs were 10 (2 alive, 7 mummy, and 1 died after birth). Among them, three live hCD73-pigs were successfully delivered by Caesarean section, but one was dead after birth. The two hCD73 TG cloned pigs had normal reproductive ability. They mated with wild type (WT) MGH (Massachusetts General Hospital) female sows and produced totally 16 piglets. Among them, 5 piglets were identified as hCD73 TG pigs. In conclusion, we successfully generated the hCD73 transgenic cloned pigs and produced their litters by natural mating. It can be possible to use a mate for the production of multiple transgenic pigs such as ${\alpha}-1,3-galactosyltransferase$ knock-out /hCD46 for xenotransplantation.

• 한국보건간호학회지
• /
• 제16권2호
• /
• pp.454-468
• /
• 2002

Historically, women's health needs have been viewed primarily as reproductive, and all other health needs have been dealth with without considering their responses to disease and treatment(Strickland & Giger, 1994). It has mostly been through the efforts of women's group, especially health care team, that more recognition has been given to the overall health of women worker in recent years. The purpose of this study was to examine the depression, self-esteem and health practice in order to identify health care strategies to improve health promotion among women workers in small workplace. The consisted of 94 women workers who work at small manufacturing industry. The data were collected from January to February, 2002. The instruments for this study was Rogenburgs self-esteem scale, Zung s depression self-reporting scale and Breslow & Enstrom s health habits scale. The analysis of data were performed with frequency, percentage, t-test, ANOVA. $\chi^2$ test, pearson correlation and multiple regression using SPSS Win 10.1 program. 1. The average depression score was 1.87 which is relatively low and the average self-esteem score was 2.75 which is relatively moderate. The total mean score for health practice was 4. 10(range 0-7) which is relatively high moderately. 2. Health practice had not a statistically significant correlation with depression and self-esteem, but the depression had a statistically significant correlation with self-esteem(r=-.401, p=.000). 3. There was not a significant difference In depression, self-esteem and health practice according to the general characteristics 4. There was a significant difference in sub category of health practice according to the status of living with family and status of marriage. As far as the health practice of women workers living with family practiced better health than women workers no living with family concerning breakfast(p=.03) and as far as the health practice of women workers no living with family practiced better health than women workers living with family concerning sleeping time(p=.04). There was a significant difference in breakfast(p=.04), smoking(p=.00), and BMI(p=.05) according to the status of marriage. 5. The major factor was age and explained for $7\%$ of health practice. The repeated study should be carried out to figure out the health practice and its related factors of female workers at small manufacturing industry.

• Reproductive and Developmental Biology
• /
• 제28권4호
• /
• pp.211-219
• /
• 2004

The effects of adenosine 5'-triphosphate (ATP) and ATP analogs, P/sub 2y/ purinoceptor agonists, on growth of normal mouse mammary epithelial cells (NMuMG) were examined. Cells were plated onto 24 well plates in DMEM supplemented with 10 % fetal calf serum. After serum starvation for 24 hours, ATP, P/sub 2y/ purinoceptor agonists (AdoPP[NH]P, ATP-α-S, ATP-γ-S, β, γ-me-ATP and 2me-S-ATP), P/sub 2u/ purinoceptor agonist (UTP) and P/sub 2y/ purinoceptor antagonists (Reactive Blue 2, more selective to P/sub 2y/ receptor than PPADS; PPADS) were added. DNA synthesis was estimated as incorporation of 3H-thymidine into DNA (1 hour pulse with 1 μ Ci/ml, 18～19 hours after treatment). ATP, Adopp[NH]P, ATP-α-S or ATP-γ-S, significantly increased DNA synthesis at 1, 10 and 100 μM concentrations with dose-dependency (P<0.05), and the maximum responses of ATP and ATP analogs were shown at 100 μM concentration (P<0.05). The potency order of DNA synthesis was ATP≥ATP- γ -S>Adopp [NH]P>ATP-α-S. β, γ -me-ATP, 2me-S-ATP and UTP did not increase DNA synthesis. In autoradiographic analysis of percentage of S-phase cells, similar results were observed to those of DNA synthesis. Addition of 1, 10 or 100 μM Reactive Blue 2 or PPADS significantly decreased ATP (100 μM)-induced DNA synthesis, however, PPADS was less effective than Reactive Blue 2. In Elvax 40P implant experiment, ATP directly stimulated mammary endbud growth in situ suggesting the physiological regulator of ATP in mammary growth. ATP 100 μM rapidly increased MAPK activity, reaching a maximum at 5 min and then gradually decreasing to the base level in 30 min. ATP analogs, Adopp[NH]P and ATP-γ-S also increased MAPK activity, however, β, γ-me-ATP and 2me-S-ATP did not. The inhibitor of the upstream MAPK kinase (MEK), PD 98059 (25 μM), effectively reduced ATP (100 μM) or EGF(10 ng/ml, as positive control)-induced MAPK activity and DNA synthesis (P<0.05). These results indicate that ATP-induced DNA synthesis was prevented from the direct inhibition of MAPK kinase pathway. Overall results support the hypothesis that the stimulatory effects of normal mouse mammary epithelial growth by addition of ATP or ATP analogs are mediated through mammary tissue specific P/sub 2y/ purinoceptor subtype, and MAPK activation is necessary for the ATP-induced cell growth.

• 한국작물학회지
• /
• 제50권1호
• /
• pp.11-15
• /
• 2005

This experiment was conducted to investigate the changes of growth and maturity and to clarify the function of supernodulating characters, excessive nodules and high biological nitrogen fixation rate (BNF), on maturity in response to different planting time in supernodulating soybean mutants. Two supernodulating soybean mutants, Sakukei4 and SS2-2, and their parent cultivars, Enrei and Shinpaldalkong2, were planted on May 24 and June 15, 2004. The degrees of the shortening of growth days by the planting time delay were 18 to 22 days in four cultivar, and there were no significant differences among the cultivars. However, four cultivars showed the different maturity properties. Sakukei4, mutated from Enrei, showed later maturity than that of Enrei, and 882-2, mutated from Shinpaldalkong2, showed earlier maturity than that of Shinpaldalkong2. The plant and nodule dry weights at R6 stage of Sakukei4 showed the smallest decrement and those of SS2-2 was showed the largest decrement by the delay of planting time. The photosynthetic rates of Sakukei4 during the late reproductive growth period were slowly decreased, however those of SS2-2 were steeply decreased in two planting time treatments. Overall, the growth of Sakukei4 was decreased slowly, however the growth of SS2-2 was decreased sharply according to the delay of planting time. The percentage of seed yield of Sakukei4 in June planting plot compared with May planting plot at R8 stage was $92\%$, which was the lowest decreasing rate of yield among the cultivars, and in the case of SS2-2, it was in $76\%$, the highest one. These results indicated that the responses of supernodulating mutants by the delay of planting time were very similar to the wild types. This means supernodulating characters in supernodulating soybean mutants might not affect to the maturity property. Additionally, the maturity property could be considered as an important characteristics to decide or to select on the developments of supernodulating soybean mutants, which have a low productivity by an excessive nodules, especially.

• 한국작물학회지
• /
• 제61권2호
• /
• pp.87-91
• /
• 2016

벼의 일조부족에 대한 생리적 반응을 조사하기 위하여 유수형성기와 수잉기에 각각 40%, 70% 차광처리를 출수직전까지 처리한 결과를 요약하면 다음과 같다. 1. 유수형성기와 수잉기에 차광처리후 출수기 간장은 차광처리가 유의하게 길어졌고, 수장은 유의하게 감소하였다. 2. 출수 후 건물생산량은 무처리에 비하여 차광 처리구에서 현저하게 감소하였으며 유수형성기 처리가 수잉기 처리에 비하여 유의한 감소를 보였고, 40% 차광처리보다 70% 차광처리에서 현저하게 감소하였다. 3. 차광처리구의 출수 후 잎의 엽색도와 질소 함량은 무처리에 비하여 유수형성기와 수잉기 처리 모두 차광 처리 정도가 높을수록 높아졌다. 4. 출수 후 20일에 벼 식물체 도복지수는 차광처리가 현저히 높아졌으며, 유수형성기 처리구가 수잉기 처리보다 유의하게 높아졌다. 5. 차광처리는 수량구성요소 중에서 이삭수와 이삭당 영화수를 감소시켰으며, 등숙률과 천립중을 감소시킴으로서 수량을 유의하게 감소시켰다. 6. 차광처리는 쌀의 단백질 함량을 높였으며 아밀로스 함량은 감소폭이 크지 않았다.