• Clinical and Experimental Reproductive Medicine
• /
• 제38권2호
• /
• pp.103-108
• /
• 2011

Objective: To investigate adverse pregnancy outcomes in non-obese women with polycystic ovary syndrome (PCOS) compared with obese-PCOS and control groups. Methods: Women with PCOS who underwent assisted reproductive technology (ART) from August, 2003 to December, 2007, were considered. A total of 336 women with PCOS were included in the study group and 1,003 infertile women who had tubal factor as an indication for ART were collected as controls. They were divided into four groups: a non-obese PCOS group, obese-PCOS group, non-obese tubal factor group, and obese tubal factor group, with obesity defined by a body mass index over 25 kg/$m^2$, and reviewed focusing on the basal characteristics, ART outcomes, and adverse pregnancy outcomes. Results: There was no difference among the groups' the clinical pregnancy rate or live birth rate. Regarding adverse pregnancy outcomes, the miscarriage rate, multiple pregnancy rate, and prevalence of preterm delivery and pregnancy induced hypertension were not different among the four groups. The incidence of small for gestational age infant was higher in the PCOS groups than the tubal factor groups ($p$ <0.02). On the other hand, the morbidity of gestational diabetes mellitus (GDM) was not high in the non-obese PCOS group but was in the obese groups. And in the obese PCOS group, the newborns were heavier than in the other groups ($p$ <0.02). Conclusion: Non-obese PCOS presents many differences compared with obese PCOS, not only in the IVF-parameters but also in the morbidity of adverse pregnancy outcomes, especially in GDM and fetal macrosomia.

• Clinical and Experimental Reproductive Medicine
• /
• 제36권1호
• /
• pp.71-80
• /
• 2009

목 적: 다낭성 난소 증후군은 생식기 여성에서 나타나는 가장 흔한 내분비 질환 중 하나이며 다양한 임상 양상을 보이고 있다. 본 연구에서는 한국인 다낭성 난소 증후군 환자 특유의 임상 양상 및 혈액학적 소견을 밝히고자 하였으며 그 결과를 터키와 미국의 다낭성 난소 증후군 환자와 비교 분석하였다. 연구방법: 2000년 1월부터 2008년 4월까지 경북대학교 병원 불임치료실을 찾은 88명의 다낭성 난소 증후군 환자를 대상으로 하여 후향적으로 임상 양상 및 혈액학적 소견을 연구하였다. 결 과: 한국인 다낭성 난소 증후군 환자군 내에서는 통계학적으로 유의한 연령에 따른 임상 양상의 차이가 없었으나 터키와 미국의 다낭성 난소 증후군 환자와 비교했을 때 고안드로겐혈증의 빈도는 유의하게 낮았다. 결 론: 다낭성 난소 증후군 환자 내에서도 인종간의 임상 양상 및 혈액학 소견의 차이가 있다고 생각되며 유전 및 생활 습관의 차이 등이 그 원인을 설명할 수 있을 것이다. 그러나 연구 대상인 환자의 수가 적고 연령군의 분포가 좁아 향후 좀 더 큰 규모의 연구가 필요하다.

• 대한수의학회지
• /
• 제44권3호
• /
• pp.441-448
• /
• 2004

DNase activity in Haemonchus contortus reproductive tissue was characterized and compared to that in whole worm. DNase activity in reproductive tissue was detected throughout pHs 4-10 with high activity under acidic conditions. The activity was not inhibited by 10 mM EDTA at pH 5.0, but largely inhibited by pH 7.0. The activity produced DNA fragments with mixtures of 3'-hydroxyls (OH) and 3'- phosphates (P) at each pH. Three distinct DNase activities were identified and had $M_rs$ of 34, 36 and 38.5 kDa in zymograms, which were distinguished according to pH requirement and sensitivity to EDTA. Among them, the 36 kDa reproductive tissue DNase had predominant activity at pH 5.0, but very weak at pH 7.0, and this activity was not inhibited by EDTA at pH 5.0. These characteristics of the 36 kDa reproductive tissue DNase resemble those of classic acidic DNases. In contrast, 36 kDa whole worm DNase activity had high activity at both pH 5.0 and 7.0. While the 36 kDa DNase activity at pH 5.0 was similar in both reproductive tissue and whole worm samples, the activity at pH 7.0 was predominantly detected in whole worm sample. This suggests that the 36 kDa whole worm DNase at pH 5.0 differs from that at pH 7.0. Thus, results indicate that the EDTA-insensitive 36 kDa DNase at pH 5.0 is specific for H. contortus reproductive tissue.

• Animal Bioscience
• /
• 제37권5호
• /
• pp.832-838
• /
• 2024

Objective: The use of molecular genetic methods in pig breeding can significantly increase the efficiency of breeding and breeding work. We applied the F_st (fixsacion index) method, the main focus of the work was on the search for common options related to the number of born piglets and the weight of born piglets, since today the urgent task is to prevent a decrease in the weight of piglets at birth while maintaining high fertility of sows. Methods: One approach is to scan the genome, followed by an assessment of Fst and identification of selectively selected regions. We chose Large White sows (n = 237) with the same conditions of keeping and feeding. The data were collected from the sows across three farrowing. For genotyping, we used GeneSeek GGP Porcine HD Genomic Profiler v1, which included 68,516 single nucleotide polymorphisms evenly distributed with an average spacing of 25 kb (Illumina Inc, San Diego, CA, USA). Results: Based on the results of the Fst analysis, 724 variants representing selection signals for the signs BALWT, BALWT1, NBA, and TNB (weight of piglets born alive, average weight of the 1st piglets born alive, total number born alive, total number born). At the same time, 18 common variants have been identified that are potential markers for both the number of piglets at birth and the weight of piglets at birth, which is extremely important for breeding work to improve reproductive characteristics in sows. Conclusion: Our work resulted in identification of variants associated with the reproductive characteristics of pigs. Moreover, we identified, variants which are potential markers for both the number of piglets at birth and the weight of piglets at birth, which is extremely important for breeding work to improve reproductive performance in sows.

• 한국수산과학회지
• /
• 제55권6호
• /
• pp.927-937
• /
• 2022

The reproductive characteristics of Pacific anchovy, Engraulis japonicus were investigated using samples randomly collected from the southern waters of Korea between January and December 2021. We analyzed monthly changes in maturity stages, gonadosomatic index (GSI), egg diameter, fecundity, and fork length (FL) at sexual groups maturity of 50%, 75% and 97.5%, and sex ratio (female:male). GSI value showed two peaks in May and August; however, microscopic analysis of E. japonicus gonad tissue revealed that the spawning period was from March to October. The main spawning seasons for the entire populations were from April to June and August to September. The overall sex ratio was female-biased (1 females, 0.89 male). The fecundity (F) of female anchovies ranged from 429 eggs at an FL of 8.0 cm to 13,824 eggs at an FL of 14.9 cm and the relationship between FL and F was 0.0788FL^4.422. The FL at 50% group maturity was 8.26 cm for female anchovies. Based on the results, we suggest that the reproductive cycle in Pacific anchovies may be related to pulses in water temperature and the sexual cycle of the fish.

• Clinical and Experimental Reproductive Medicine
• /
• 제39권3호
• /
• pp.114-117
• /
• 2012

Objective: It is well known that fresh blastocyst transfer results in better pregnancy outcomes with a smaller number of transferred embryos compared with cleavage stage embryo transfer. However, in terms of frozen-thawed blastocyst transfer, only a few studies are available. We aimed to evaluate clinical outcomes of frozen-thawed embryo transfer (FET) with blastocysts. Methods: Retrospective analysis of FET cycles with blastocysts (B-FET) between Jan 2007 and June 2009 was performed. Age-matched FET cycles with cleavage stage embryos (C-FET) during the same period were collected as controls. A total of 58 B-FET cycles were compared with 172 C-FET cycles and also compared with those of post-thaw extended culture blastocysts from frozen pronuclear stage embryos (22 cycles). Results: There was no difference in the patient characteristics of each group. The embryos' survival rates after thawing were comparable (>90%) and there was no difference in the implantation rate or clinical and ongoing pregnancy rate among the three groups. Conclusion: In FET, blastocyst transfers may not present better pregnancy outcomes than cleavage stage embryo transfers. A further large-scale prospective study is needed.

• Clinical and Experimental Reproductive Medicine
• /
• 제48권3호
• /
• pp.262-267
• /
• 2021

Objective: Progesterone application for luteal phase support is a well-established concept in in vitro fertilization (IVF) treatment. Water-soluble subcutaneous progesterone injections have shown pregnancy rates equivalent to those observed in patients receiving vaginal administration in randomized controlled trials. Our study aimed to investigate whether the results from those pivotal trials could be reproduced in daily clinical practice in an unselected patient population. Methods: In this retrospective cohort study in non-standardized daily clinical practice, we compared 273 IVF cycles from 195 women undergoing IVF at our center for luteal phase support with vaginal administration of 200 mg of micronized progesterone three times daily or subcutaneous injection of 25 mg of progesterone per day. Results: Various patient characteristics including age, weight, height, number of oocytes, and body mass index were similar between both groups. We observed no significant differences in the clinical pregnancy rate (CPR) per treatment cycle between the subcutaneous (39.9%) and vaginal group (36.5%) (p=0.630). Covariate analysis showed significant correlations of the number of transferred embryos and the total dosage of stimulation medication with the CPR. However, after adjustment of the CPR for these covariates using a regression model, no significant difference was observed between the two groups (odds ratio, 0.956; 95% confidence interval, 0.512-1.786; p=0.888). Conclusion: In agreement with randomized controlled trials in study populations with strict selection criteria, our study determined that subcutaneous progesterone was equally effective as vaginally applied progesterone in daily clinical practice in an unselected patient population.

• Clinical and Experimental Reproductive Medicine
• /
• 제38권4호
• /
• pp.228-233
• /
• 2011

Objective: To investigate the effectiveness of GnRH antagonist multiple-dose protocol (MDP) with oral contraceptive pill (OCP) pretreatment in poor responders undergoing IVF/ICSI, compared with GnRH antagonist MDP without OCP pretreatment and GnRH agonist low-dose long protocol (LP). Methods: A total of 120 poor responders were randomized into three groups according to controlled ovarian stimulation (COS) options; GnRH antagonist MDP after OCP pretreatment (group 1), GnRH antagonist MDP without OCP pretreatment (group 2) or GnRH agonist luteal low-dose LP without OCP pretreatment (group 3). Patients allocated in group 1 were pretreated with OCP for 21days in the cycle preceding COS, and ovarian stimulation using recombinant human FSH (rhFSH) was started 5 days after discontinuation of OCP. Results: There were no differences in patients' characteristics among three groups. Total dose and days of rhFSH used for COS were significantly higher in group 3 than in group 1 or 2. The numbers of mature oocytes, fertilized oocytes and grade I, II embryos were significantly lower in group 2 than in group 1 or 3. There were no significant differences in the clinical pregnancy rate and implantation rate among three groups. Conclusion: GnRH antagonist MDP with OCP pretreatment is at least as effective as GnRH agonist low-dose LP in poor responders and can benefit the poor responders by reducing the amount and duration of FSH required for follicular maturation.

• 대한생식의학회:학술대회논문집
• /
• 대한불임학회 1994년도 추계 학술대회 및 정기총회
• /
• pp.20.1-20.1
• /
• 1994

• Clinical and Experimental Reproductive Medicine
• /
• 제3권1호
• /
• pp.5-11
• /
• 1976

Considerable data has been reported on the outcome of pregnancies subsequent to induced abortion, but the findings contain a great deal of inconsistency and disagreement. Most studies strongly suggest that normal deliveries are less likely to occur in subsequent pregnancies following induced abortion, in terms of gestation length, birth weight, stillbirth, and miscarriage. Other work suggests that some of the demographic and health characteristics of women who experience induced abortion are different from those women who do not; and these factors may affect the outcome of subsequent pregnancies profoundly rather than the induced abortion itself.