• Title/Summary/Keyword: Reproduction experiments

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A Putative Transcription Factor pcs1 Positively Regulates Both Conidiation and Sexual Reproduction in the Cereal Pathogen Fusarium graminearum

  • Jung, Boknam;Park, Jungwook;Son, Hokyoung;Lee, Yin-Won;Seo, Young-Su;Lee, Jungkwan
    • The Plant Pathology Journal
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    • v.30 no.3
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    • pp.236-244
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    • 2014
  • The plant pathogen Fusarium graminearum causes Fusarium head blight in cereal crops and produces mycotoxins that are harmful to animals and humans. For the initiation and spread of disease, asexual and sexual reproduction is required. Therefore, studies on fungal reproduction contribute to the development of new methods to control and maintain the fungal population. Screening a previously generated transcription factor mutant collection, we identified one putative $C_2H_2$ zincfinger transcription factor, pcs1, which is required for both sexual and asexual reproduction. Deleting pcs1 in F. graminearum resulted in a dramatic reduction in conidial production and a complete loss of sexual reproduction. The pathways and gene ontology of pcs1-dependent genes from microarray experiments showed that several G-protein related pathways, oxidase activity, ribosome biogenesis, and RNA binding and processing were highly enriched, suggesting that pcs1 is involved in several different biological processes. Further, overexpression of pcs1 increased conidial production and resulted in earlier maturation of ascospores compared to the wild-type strain. Additionally, the vegetative growth of the overexpression mutants was decreased in nutrient-rich conditions but was not different from the wild-type strain in nutrient-poor conditions. Overall, we discovered that the pcs1 transcription factor positively regulates both conidiation and sexual reproduction and confers nutrient condition-dependent vegetative growth.

A Study on the Color Reproduction for Offset Printing using Ecological Ink in the Domestic Printing Environment (국내 인쇄 환경에서 친환경 잉크를 이용한 오프셋 인쇄의 색재현에 관한 연구)

  • Moon, Sung-Hwan;Kim, Sung-Su;Koo, Chul-Whoi;Yoo, Keun-Ryong
    • Journal of the Korean Graphic Arts Communication Society
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    • v.28 no.2
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    • pp.69-85
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    • 2010
  • Currently, environmental contaminants that can cause Aromatic types of hydrocarbons, less than 1% made of Aromatic Free kind of used products, soybean oil products with linseed oil with the products, rice products using a wide range of environmentally ecological ink since 2000 is released quickly. All materials used in printed material, if the green is the best way to print the composite materials in industrial applications, because each process on the print quality and productivity, there can be many differences in this experiment because it accounts for a large proportion in the print general ink in the ink section and the International color standards(ISO2846-1:2006) certified ecological ink were compared. Therefore, in this paper has the ink released from the same company, each common general ink and ecological ink in the same condition which results were focused on whether the emerging international color standard(ISO 2846-1:2006) recognized for environmentally ecological ink printed color reproduction of the actual offset(color reproduction) how conformity to ISO 12647-2 standard color on the basis of the offset would check Color Reproduction. Based on the results of the experiments of this study, given the ecological ink coated paper, uncoated paper both color difference and the gamut of the ISO 12647-2 standard is suitable for ecological ink, the ink's color gamut reproduction, even more than existing international standards, there is no confirmed that the correct color reproduction possible. Using environmentally ecological ink industries is expected to respond to environmental policy.

Structure Design of Artificial Neural Networks using Genetic Algorithm (유전적 알고리즘을 이용한 인공신경망의 구조 설계)

  • 이재식;차봉근
    • Journal of the Korean Operations Research and Management Science Society
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    • v.24 no.3
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    • pp.49-62
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    • 1999
  • Artificial Neural Networks(ANN) have been successfully applied to various kinds of business and engineering problems, especially those involved in pattern classification. However, because of the lack of design standard or guidelines, the structure of specific ANN depends on the designer's own experiments or choices. In other words, even though we could construct a better ANN, we often steeled down with just a satisfactory ANN. The purpose of this research is to apply the Genetic Algorithm(GA) to design a structure of ANN that yields better performance compared to the existing test results. For a bankruptcy prediction problem. an exiting research using ANN which consists of 22 input processing elements(PEs) for financial ratios and 5 hidden PEs showed 70% hit ratio. In our research, the input financial ratios and the number of hidden PEs are determined by GA. The best ANN, which consists of 8 input PEs and 6 hidden PEs, shows 78.03% hit ratio. In addition, we compare the performance of two types of reproduction schemes, i.e., generational reproduction and steady-state reproduction.

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Unusual Rhizoidal Development in Bangia (Bangiales, Rhodophyta) -Another Form of Vegetative Reproduction?

  • Boedeker, Christian;Farr, Tracy J.;Nelson, Wendy A.
    • ALGAE
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    • v.22 no.1
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    • pp.31-36
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    • 2007
  • The gametophytic filaments of two genetically distinct taxa of Bangia from New Zealand showed unusual rhizoidal development in comparative culture experiments. In the past Bangia has been reported to possess simple, colourless rhizoids that extend from the basal cells of the unbranched filaments, whereas in this study the rhizoids observed became pigmented and multicellular. A reversal of growth direction occurred and filamentous extensions developed from the rhizoids under some culture conditions. These extensions were either prostrate or resembled new gametophytic filaments. This is the first report for filamentous members of the Bangiales of the development of such stolon-like rhizoids, apparently serving as a form of vegetative reproduction.

In Vivo Transfer of Foreign DNA into Primordial Germ Cells (PGCs) of Chicken Embryos

  • Eguma, K.;Soh, T.;Hattori, M.;Fujihara, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.4
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    • pp.520-524
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    • 1999
  • The present experiments were designed to examine whether exogenous DNA injected into the germinal crescent region (GCR) of early stage of developing embryos, which is considered to be the main place from which PGCs originate, can be transferred to recipient chicken embryos. In this experiment, Miw Z (DNA) dissolved in the transfection reagent (TR: Boehringer, Germany) was introduced into the GCR of donor embryos at stage 3-5 or 9-11, followed by continued incubation until the stage 13-15 of embryonic development. The PGCs collected from the embryonic blood vessels were examined for the incorporation of the injected DNA into the PGCs by the methods of X-gal staining and PCR analysis. As the results, the foreign DNA was successfully incorporated into the PGCS, leading to their transfer to the gonadal tissues. The present results, therefore, suggest that the early stage (3-5 or 9-11) of chicken embryonic development would be more successful than stage 13-15 in transferring exogenous genes to the recipient embryos, leading to the possibility of producing transgenic chicken medianting the PGCS.

A Study on The Color Reproduction Properties of Domestic Newspaper Prints (국내 신문 인쇄의 색재현 특성에 대한 평가)

  • Park, Han-Areum;Kang, Sang-Hoon
    • Proceedings of the Korean Printing Society Conference
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    • 2007.11a
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    • pp.29-43
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    • 2007
  • Web offset newspaper prints is the most widely used printing process in domestic printing industry, but there are no industry-wide specifications for press control to assure consistent quality across newspaper printing plants. As newspaper printing becomes more of a commodity and less of an art, it is necessary to develop the print quality specification standard suitable for Korea web offset newspaper printing field. There are a few commonly employed printing standards such as, SNAP(Specification for Non-Heatset Publication) of the US, IFRA(INCA-FIEJ Research Association), J-color (Japan printing machinery association). And there exists KS for domestic standards, however, since it has been directly imported from ISO standards, there are discrepancies in printing materials and media. This paper aims to examine the color reproduction quality of domestic web offset newspaper prints by comparing with foreign standards. Printing experiments on this study have been performed at one of the domestic newspaper printing companies. The color reproduction properties of the web offset newspaper prints are measured by means of densitometry and colorimetry. These measures are evaluated comapratively with the international standards by means of analyzing, the major factors that determine the color reproduction quality of web offset newspapar prints.

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A Study on The Color Reproduction Properties of Domestic Newspaper Prints (국내 신문 인쇄의 색재현 특성에 관한 연구)

  • Park, Han-A-Reum;Kang, Sang-Hoon
    • Journal of the Korean Graphic Arts Communication Society
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    • v.25 no.1
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    • pp.27-41
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    • 2007
  • Web offset newspaper prints is the most widely used printing process in domestic printing industry, but there are no industry-wide specifications for press control to assure consistent quality across newspaper printing plants. As newspaper printing becomes more of a commodity and less of an art, it is necessary to develop the print quality specification standard suitable for Korea web offset newspaper printing field. There are a few commonly employed printing standards such as, SNAP (Specification for Non-Heatset Publication) of the US, IFRA(INCA-FIEJ Research Association), J-color (Japan printing machinery association). And there exists KS for domestic standards, however, since it has been directly imported from ISO standards, there are discrepancies in printing materials and media. This paper aims to examine the color reproduction quality of domestic web offset newspaper prints by comparing with foreign standards. Printing experiments on this study have been performed at one of the domestic newspaper printing companies. The color reproduction properties of the web offset newspaper prints are measured by means of densitometry and colorimetry. These measures are evaluated comapratively with the international standards by means of analyzing, the major factors that determine the color reproduction quality of web offset newspaper prints.

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Reproduction of vibration patterns of elastic structures by block-wise modal expansion method (BMEM)

  • Jung, B.K.;Cho, J.R.;Jeong, W.B.
    • Smart Structures and Systems
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    • v.18 no.4
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    • pp.819-837
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    • 2016
  • The quality of vibration pattern reproduction of elastic structures by the modal expansion method is influenced by the modal expansion method and the sensor placement as well as the accuracy of measured natural modes and the total number of vibration sensors. In this context, this paper presents an improved numerical method for reproducing the vibration patterns by introducing a block-wise modal expansion method (BMEM), together with the genetic algorithm (GA). For a given number of vibration sensors, the sensor positions are determined by an evolutionary optimization using GA and the modal assurance criterion (MAC). Meanwhile, for the proposed block-wise modal expansion, a whole frequency range of interest is divided into several overlapped frequency blocks and the vibration field reproduction is made block by block with different natural modes and different modal participation factors. A hollow cylindrical tank is taken to illustrate the proposed improved modal expansion method. Through the numerical experiments, the proposed method is compared with several conventional methods to justify that the proposed method provides the improved results.

Research advances in reproduction for dairy goats

  • Luo, Jun;Wang, Wei;Sun, Shuang
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.8_spc
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    • pp.1284-1295
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    • 2019
  • Considerable progress in reproduction of dairy goats has been made, with advances in reproductive technology accelerating dairy goat production since the 1980s. Reproduction in goats is described as seasonal. The onset and length of the breeding season is dependent on various factors such as breed, climate, physiological stage, male effect, breeding system, and photoperiod. The reproductive physiology of goats was investigated extensively, including hypothalamic and pituitary control of the ovary related to estrus behavior and cyclicity etc. Photoperiodic treatments coupled with the male effect allow hormone-free synchronization of ovulation, but the kidding rate is still less than for hormonal treatments. Different protocols have been developed to meet the needs and expectations of producers; dairy industries are subject to growing demands for year round production. Hormonal treatments for synchronization of estrus and ovulation in combination with artificial insemination (AI) or natural mating facilitate out-of-season breeding and the grouping of the kidding period. The AI with fresh or frozen semen has been increasingly adopted in the intensive production system, this is perhaps the most powerful tool that reproductive physiologists and geneticists have provided the dairy goat industry with for improving reproductive efficiency, genetic progress and genetic materials transportation. One of the most exciting developments in the reproduction of dairy animals is embryo transfer (ET), the so-called second generation reproductive biotechnology following AI. Multiple ovulation and ET (MOET) program in dairy goats combining with estrus synchronization (ES) and AI significantly increase annual genetic improvement by decreasing the generation interval. Based on the advances in reproduction technologies that have been utilized through experiments and investigation, this review will focus on the application of these technologies and how they can be used to promote the dairy goat research and industry development in the future.

Effects of Various Cryoprotectants on the Survival of Frozen Mouse Embryo (항동해제의 종류가 동결 생쥐배의 생존성에 미치는 영향)

  • Rho, H.C.;Pek, U.H.;Lee, K.W.;Koh, D.H.;Chung, K.S.
    • Korean Journal of Animal Reproduction
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    • v.10 no.2
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    • pp.175-181
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    • 1986
  • These experiments were carried out to clarify the effects various kinds of cryoprotectants which were frequently used in freezing embryos of domestic animals on the survival of frozen-thawed mouse embryos. As cryoprotectant, glycerol, DMSO and methanol were used and the procedures of adding them in medium were practiced by one-step or six-step adding method. Morphologically normal mouse embryos developed to blastocyst by in vitro culture after freezing and thawing were transferred to pseudopregnant recipients by surgical procedures. The results obtained in these experiments were summarized as follows: 1. The survival rates of the frozen-thawed 8-cell embryos, morulas and blastocysts following one-step addition of glycerol were 83.6, 80.3 adn 70.3%, respectively, while following six-step addition of glycerol, 69.2, 56.3 and 66.7% respectively. 2. When glycerol, DMSO and methanol were used as cryoprotectant under the same condition of freezing and thawing, the survival rates of frozen-thawed embryos were 74.0, 76.1 and 37.6%, respectively. 3. The implantation rate of embryos transferred to pseudopregnant recipients after freezing and thawing was 49.2%.

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