Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

In Vivo Transfer of Foreign DNA into Primordial Germ Cells (PGCs) of Chicken Embryos

  • Eguma, K. (Laboratory of Animal Reproduction, Faculty of Agriculture, Kyushu University) ;
  • Soh, T. (Laboratory of Animal Reproduction, Faculty of Agriculture, Kyushu University) ;
  • Hattori, M. (Laboratory of Animal Reproduction, Faculty of Agriculture, Kyushu University) ;
  • Fujihara, N. (Laboratory of Animal Reproduction, Faculty of Agriculture, Kyushu University)
  • Received : 1998.12.07
  • Accepted : 1999.01.23
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The present experiments were designed to examine whether exogenous DNA injected into the germinal crescent region (GCR) of early stage of developing embryos, which is considered to be the main place from which PGCs originate, can be transferred to recipient chicken embryos. In this experiment, Miw Z (DNA) dissolved in the transfection reagent (TR: Boehringer, Germany) was introduced into the GCR of donor embryos at stage 3-5 or 9-11, followed by continued incubation until the stage 13-15 of embryonic development. The PGCs collected from the embryonic blood vessels were examined for the incorporation of the injected DNA into the PGCs by the methods of X-gal staining and PCR analysis. As the results, the foreign DNA was successfully incorporated into the PGCS, leading to their transfer to the gonadal tissues. The present results, therefore, suggest that the early stage (3-5 or 9-11) of chicken embryonic development would be more successful than stage 13-15 in transferring exogenous genes to the recipient embryos, leading to the possibility of producing transgenic chicken medianting the PGCS.

Keywords

Cited by

  1. Gene Transfer to Chicken Blastoderm by Lipofection or Electroporation vol.17, pp.2, 2000, https://doi.org/10.1080/09712119.2000.9706304
  2. Introduction of Foreign Gene into the Gonads via Primordial Germ Cells Originated from Germinal Crescent of Chicken Embryos vol.18, pp.1, 2000, https://doi.org/10.1080/09712119.2000.9706321
  3. Genetic Resource Conservation in Poultry Reproduction vol.19, pp.1, 2001, https://doi.org/10.1080/09712119.2001.9706708
  4. Transfer of blood containing primordial germ cells between chicken eggs development of embryonic reproductive tract vol.56, pp.1, 2008, https://doi.org/10.1007/s10616-007-9096-x
  5. Expression of mRNA for 3HADH in manipulated embryos to produce germline chimeric chickens vol.60, pp.1-3, 2009, https://doi.org/10.1007/s10616-009-9232-x
  6. Effect of Transfer Primordial Germ Cells (PGCs) into Chick Gonad vol.44, pp.3, 2007, https://doi.org/10.2141/jpsa.44.335
  7. Successful Transfer of Exogenously Introduced Gene (lacZ/MiwZ and lacZ&GFP/pkkv4-lacZ) for Generations in Chicken vol.46, pp.3, 1999, https://doi.org/10.1262/jrd.46.177
  8. In Vivo Gene Transfer into Chicken Embryos via Primordial Germ Cells Using Green Fluorescent Protein as a Marker vol.46, pp.2, 1999, https://doi.org/10.1262/jrd.46.79
  9. Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs) vol.38, pp.4, 1999, https://doi.org/10.2141/jpsa.38.302
  10. Establishing Germline Chimeric Chickens Using Primordial Germ Cells vol.49, pp.1, 1999, https://doi.org/10.2141/jpsa.011053