• Korean Journal of Animal Reproduction
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• v.14 no.1
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• pp.1-8
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• 1990

These experiments were carried out to investigate the effects of cumulus cells for in vitro fertilization and development of bovine follicular oocytes matured in vitro. The bovine ovaries were obtained at a slaughter house and the follicular oocytes surrounded by cumulus cells were collected by puncturing follicles with 2~6 mm of diameter. Bovine oocytes were matured in vitro for 24~26 hours in a CO2 incubator with 5% CO2 in air at 39$^{\circ}C$. The medium used for maturation was TCM-199 supplemented with hormones, pyruvate, FCS and antibiotics. Epididymal spermatozoa were capacitated by in vitro culture for 2~3 hours in BO solution containing BSA(5mg/ml) and caffeine(2.5mM). Insemination was made by introducing about 10~15 matured oocytes into the suspension of capacitated spermatozoa. Six hour after insemination the eggs were transferred to TCM-199 supplemented with FCS(10%) and HEPES(25mM), cultured for 7~8 days with 10~15 eggs/well in 4-well multidishes(Nunc Co.) forming cumulus cell monolayer. The results obtained in these experiments were summarized as follows ; 1. The majority of the follicular oocytes with compacted cumulus cells existed in GV stage while those with dispersed or denuded cumulus cells existed GVBD and M II stage. 2. After 24~26 hours maturation, the maturation rates of the follicular oocytes cultured in TCM-199 containing hormones were slightly higher than those of oocytes cultured in medium without hormones, and the frequency of cumulus compacted or denuded oocytes reaching M II stage cultured in medium containing hormones was 75.7% or 51.7%, respectively(P<0.05). 3. After 20 hours in vitro insemination, percentages of ova fertilized were 61.4% or 51.4%, respectively, for cumulus oophorus intacted or removed, and increased frequency of ova with both male and female pronuclei was found when cumuli were present(P<0.05). 4. The rates of embryos developed to 2-, 4-, 8-, 16-cell and morula or blastocyst stage after cocultured with cumulus cells were 65.0%, 45.3%, 34.7%, 28.0% and 22.7%, respectively. The results for momla or blastocyst stage were significantly higher than those of the embryos cultured in the basic medium(P<0.05).

• The Journal of the Acoustical Society of Korea
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• v.24 no.2
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• pp.68-77
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• 2005

The purpose of a spatial audio system is to give a listener an impression as if he were present in a recorded environment when its sound is reproduced. For this purpose a dummy head microphone is generally used. Because of its human-like shape, dummy head microphone can reproduce spatial images through headphone reproduction. However, its shape and size are restriction to public use and it is difficult to convert the output signal of dummy head microphone into a multi-channel signal for multi-channel environment. So, in this paper, we propose a multi-channel 3D microphone technology. The multi-channel 3D microphone acquire a spatial audio using five microphones around a horizontal plane of a rigid sphere and through post processing, it can reproduce various reproduction signals for headphone, stereo, stereo dipole, 4ch and 5ch reproduction environments. Because of complex computation, we implemented H/W based post processing system. To verily the Performance of the multi-channel 3D microphone, localization experiments were Performed. The result shows that a front/back confusion, which is the one of common limitations of conventional dummy head technology, can be reduced dramatically.

• Korean journal of applied entomology
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• v.53 no.2
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• pp.141-147
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• 2014

Pinewood nematode (PWN: Bursaphelenchus xylophilus) is known to kill pine tree species that are indigenous to countries where the pest was inadvertently imported, but some cultures from the extraction of dead pines do not damage trees. Experiments were conducted to examine the effect of pre-inoculation of these low-pathogenic pinewood nematode on resistance of pine trees against the pest species. The pre-inoculated pine saplings showed induced resistance which lasted for a year, and repeated inoculation of these low-pathogenic nematodes enhanced tree resistance. All nematode samples extracted from dying or dead pines that had been killed not more than three months before the extraction were pathogenic, and most of those extracted from pines that had been killed 2-3 years before were low-pathogenic. When inoculated in pine saplings, number of low-pathogenic nematodes settled, as studied two days after inoculation, was not different from that of pathogenic ones. However, as studied after 30 days of inoculation, rate of reproduction in low-pathogenic nematodes was far lower than that of pathogenic nematodes. The rate of reproduction of several nematode isolates growing on fungal mat media of Botrytis cinerea varied, but three of four low-pathogenic isolates showed same level of reproduction rates as pathogenic ones.

• Journal of the Korea Computer Graphics Society
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• v.28 no.3
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• pp.67-77
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• 2022

The sense of touch by natural wind is one of the most common feels that every person experiences in daily life. However, it has been rarely studied how natural wind can be reproduced in a VR environment and whether the multisensory contents equipped with artificial winds do improve human emotion or not. To address these issues, we first propose a wind reproduction VR system guided by video and wind capture data and also study the effect of the system on positive affect. We collected wind direction and speed data together with a 360-degree video on a yacht. These pieces of data were used to produce a multisensory VR environment by our wind reproduction VR system. 19 college students participated in the experiments, where the Korean version of Positive and Negative Affect Schedule (K-PANAS) was introduced to measure their emotions. Through the K-PANAS, we found that 'inspired' and 'active' emotions increase significantly after experiencing the yacht VR contents with artificial wind. Our experimental results also show that another emotion, 'interested', is most notably affected depending on the presence of the wind. The presented system can be effectively used in various VR applications such as interactive media and experiential contents.

• Korean Journal of Heritage: History & Science
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• v.56 no.3
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• pp.118-135
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• 2023

Hanging boards of the Joseon royal court are hung on buildings related to the royal family, such as palaces and Jongmyo Shrine, to show the hierarchy and character of the building. In addition, the manufacturing method and materials are recorded in the royal protocols of the Joseon Dynasty, so it is an important material for studying the manufacturing method and material changes at that time. However, the hanging boards were restored several times due to fire or war, and it is presumed that there is a change in the original form and material of the hanging boards. In particular, many hanging boards of the Joseon royal court were written with calligraphy by kings, so there are many forms consisting of gold letters on a black background. This study tried to analyze the pigments remaining in the letters of 44 of the Joseon royal hanging boards, which are presumed to be gold letters, and to find out the changes in the hanging board production method and materials by referring to the analysis results. The letters of the hanging boards studied were classified according to the current state of the gold pigment and the detected components. As a result of the analysis of character pigments, 24 embossing techniques and 5 intaglio techniques were mainly detected with gold (Au), but 15 embossing techniques were detected with brass (Cu, Zn). Only blue-green substances, not gold pigments, remain in some of the hanging boards in which brass components were detected. A reproduction experiment was conducted because the pigments of the brass component were not recorded in the literature and were not currently used as Dancheong pigments. In the reproduction experiment, it was difficult to confirm the application and use of brass pigments due to the limitations of materials, but it is judged that research on the timing and method of using brass pigments is needed in the future.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 1993.06a
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• pp.4-5
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• 1993

Toxicity tests in our laboratory are conducted usually with mass-reared organisms. They are under the same environmentel conditions throughout seasons and are supplied at specific age. A total of 38 species of aquatic organisms are being reared. We have attempted to establish pruified strains or to select clones of various parthenogenic organisms. The merits or demerits of our culturing test organisms are discussed. The differences in the susceptibility among clones or strains of test organism are also discussed. For a single species test, algae, daphnia, fish are often used. However, we usually use early stages, but occasionally, adults fish are used for reproduction tests. As an another important aspect, the toxicity through food chains has been studied. In this study, we select a pair of species belonging different trophic levels. The differences between single species tests and multispecies tests will be discussed. Even a single species test intends to assess the effects of chemicals on ecosystem levels, however, this idea is not applicable to ecosystems. Single species tests with standard organisms and multispecies tests are contradictory in concept. One type of multispecles tests is indoor microcosms being composed of severel species artificially assembled, and another is composed of natural components (both indoor and outdoor). We have used three types of outdoor mesocosms using ponds and three types of artificial streams. The mesocosms is useful to not only to analyze the floral or faun61 changes but also to study the fate or behaviour of chemicals in naturd environments. Lastly, usefulness of the field observation or experiments or semi-field experiments will be discussed. This will enhance the exploitation of early warning systems utilizing indicator organisms or animal behaviour.aviour.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.180-187
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• 2012

Panicum maximum, Panicum repens, Imperata cylindrica, Pennisetum polystachion and Cyperus rotundus are five rhizomatous grass weeds that exist and are problematic in the coconut lands of Sri Lanka. Rhizomatous weeds are the most difficult to control because of their vegetative reproduction by underground propagules. Therefore chemical and biological techniques have failed to control these weeds to acceptable levels. Experiments were conducted to investigate the impact of depth of burial, duration of sun drying or duration of air drying rhizomes of the selected weed species on germination. Depth of burial reduced germination of all species. Burying rhizomes at depths less than 10 cm inhibited germination of P. maximum and P. polystachion. Burying depths below 30~40 cm inhibited germination of all species. Sun drying or air drying rhizomes for durations less than 5 days inhibited germination of P. maximum and P. polystachion. Germinations of all species were inhibited by sun drying rhizomes for 15 days or air drying rhizomes for 20 days. Sun drying of rhizomes of all species for five days reduced the moisture content to a greater extent than air drying. The results indicated that burying rhizomes at the depthsbelow 30~40 cm, sun drying rhizomes for durations beyond 15 days or air drying rhizomes for durations beyond 20 days would be effective in controlling germination of these species. The investigations of the experiments also suggest that keeping rhizomes on the soil surface without burying, sun drying rhizomes or air drying rhizomes for durations of 5~15 days would produce weak plants.

• Journal of Ocean Engineering and Technology
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• v.27 no.5
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• pp.105-114
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• 2013

Lots of orders of special vessels and offshore plants for developing the resources in deepwater have been increased in recent. Because the most of accidents on those structures are caused by fire and explosion, many researchers have been investigated quantitatively to predict the cause and effect of fire and explosion based on both experiments and numerical simulations. The first step of the evaluation procedures leading to fire and explosion is to predict the dispersion of flammable or toxic material, in which the released material mixes with surrounding air and be diluted. In particular turbulent mixing, but density differences due to molecular weight or temperature as well as diffusion will contribute to the mixing. In the present paper, the numerical simulation of hydrogen dispersion inside a simple-shaped offshore structure was performed using a commercial CFD program, ANSYS-CFX. The simulated results for concentration of released hydrogen are compared to those of experiment and other simulation in Jordan et al.(2007). As a result, it is seen that the present simulation results are closer to the experiments than other simulation ones. Also it seems that the hydrogen dispersion is closely related to turbulent mixing and the selection of the turbulence model properly is significantly of importance to the reproduction of dispersion phenomena.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.329-329
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• 2017

In this study attempted to evaluate the stability of the protection methods by examining hydraulic characteristics of the area around the point in which marine cable protector is installed such as surf zone occurrence point of shore-end submarine cables suitable for coastal marine environmental conditions, flow rate t the tope of the protector and maximum wave height, and to provide basic data for the selection of the optimal protection method. In performing hydraulic model experiments, the topography of submarine cable installation location was reproduced in 2-D sectional channel, and models appropriate for experimental scale and similitude law were produced and installed for each condition of submarine cables and protectors. Since the topography and submarine cable protectors were reproduced and installed in 2-D sectional channel, the exact reproduction of surf and transformation in shallow water zone was possible, and thus the physical properties could be clearly analyzed. For stability review, an experiment to examine the stability was conducted using a wave maker with 50-year frequency design waves as target, and wave height and cycles were applied based on the approximate lowest low water level(Approx. L.L.W), which is the most dangerous in submarine cable protection methods. As for experimental time, typhoon passing time in summer (about 3 hours) was applied, and wave patterns and deviation ratio of the submarine cable protector were investigated after making irregular waves corresponding to design waves. In addition, current meter and wave height meter were installed at the installation location of the submarine cable protector, and the flow rates and wave height at the top of the protector were measured and analyzed to review hydraulic properties.

• Development and Reproduction
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• v.27 no.3
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• pp.149-157
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• 2023

We investigated the involvement of autophagy with steroidogenesis in testicular Leydig cells. Human chorionic gonadotropin (hCG)-stimulated T production in Leydig cells was not remarkably altered in the presence of an autophagy inhibitor 3-methyladenine (3-MA). Although pretreatment with 3-MA demonstrated a tendency to decrease hCG-induced T production, the differences were significant only at a higher time point of 24 h following hCG. Microtubule associated protein light chain 3 (LC3)-II was detectable in the control cells in all the experiments. The hCG-induced increase in steroidogenic acute regulatory protein (StAR) and cytochrome P450 side chain cleave (P450scc) protein levels were not significantly altered by 3-MA. Leydig cells isolated from immature rat testes 12 h following hCG treatment showed relatively increased levels of LC3-II protein compared to the control group. Furthermore, LC3-II levels shown in these cells reached almost the identical to those from normal adult testes. However, LC3-II protein levels were almost comparable or even slightly lower than the controls at 48 h following hCG. Expression of StAR and P450scc was upregulated at both 12 and 48 h after hCG. We also used MA-10 cells, the mouse Leydig cell line, in this experiment. When dibutyryl cyclic-AMP was treated with MA-10 cells, P4 levels were significantly increased in the cell culture medium. However, P4 levels tended to decrease in the presence of 3-MA, but the difference was not statistically significant. This was consistent with the results of the rat Leydig cell experiments. Together, we believe that although autophagy participates in steroidogenesis and enhances steroidogenic efficacy of Leydig cells, it may not be a decisive cellular process for steroidogenesis, specifically in the mature Leydig cells.