• 식물병연구
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• 제10권1호
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• pp.25-29
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• 2004

구미시 옥성면 국화 집단재배지에서 국화 생육불량 원인을 조사한 결과, 대부분의 국화(98％)가 뿌리혹선충(Meloidogyne hapla)에 감염되어 있었다. 주요 3품종 중 뿌리에 생긴 난낭의 수는 뿌리 1 g당 'Ardilo' 3.3개, 'Euro' 6.5개, 'Monarisa' 17개로 'Monarisa' 품종에서 난낭 발생이 많았고 'Ardilo' 품종에서 적었다. 국화의 초장, 화경장, 줄기 굵기, 엽수, 착화수, 첫 번째 화수장, 절화중, 엽록소(SPAD), 뿌리무게, 뿌리혹선충의 난낭수 사이의 상관관계를 조사한 결과 난낭수와 엽록소 함량(SPAD) 사이에 부의 상관이 인정되었다(r = -0.418, p = 0.059; r = -0.463, p=0.040). 식물 생육은 키가 크고 화경장이 길수록 꽃수, 잎수가 많고 꽃 무게도 무거웠다(p < 0.05).

• 한국수자원학회논문집
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• 제43권11호
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• pp.933-944
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• 2010

본 논문에서는 확률강우량에 대한 공간분포 추정에 있어서 공간변동성에 따른 불확실성을 평가하기 위하여 지구통계 학적 추계모의기법인 CEM과 SGS 기법을 비교하였다. CEM과 SGS를 이용한 추계모의에 있어서 공간상관구조의 재생성, 확률강우량에 대한 불확실성 평가측도로서 실현치에 대한 통계치(표준편차, 변동계수, 사분위수 범위 및 범위)의 공간분포, 유역평균강우량의 불확실성 분포의 경우 두 기법이 대체로 비슷한 결과를 보이는 것으로 분석되었다. 그러나 모의 효율성 측면에서는 CEM이 SGS에 비해 우수한 결과를 나타내는 것으로 분석되었다.

• Animal Bioscience
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• 제34권7호
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• pp.1105-1115
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• 2021

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

• 한국가축번식학회지
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• 제8권1호
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• pp.46-55
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• 1984

The purpose of the experiment was to clarify morphologically normal growth pattern of the ductus deference in accordance with the sex maturity of meat-type cockerels. 1. Diameter of lumens in u, pp.r, mid and lower parts of ductus deferens, the most conspicuous enlargement of lumen was observed in the lower part. Heights of epithelial layers of ductus deferens showed abrupt growth at 12 weeks of age with subsequent gradual growth in all the part of u, pp.r, mid and lower, and heights of those at 30 weeks were a, pp.oximately 4 times as large in the u, pp.r and mid parts and 5 times as large in the lower part in contrast to those at 4 weeks of age. Thickness of muscular layer of ductus showed gradual growth in contrast with the diameter of lumen and height of epithelial layer, showing 1.3 times as large in the u, pp.r part, 1.6 times in the mid part and 1.9 times in the lower part at 30 weeks of age in contrast to the thickness at 4 weeks of age. 2. Within 10 weeks after hatching, lining cells of ductus deferens were mainly composed of round cells and columnar cells in simple columnar epithelium. During 10th to 20th week, the lining cells were mainly composed of high columnar cells and round cells in pseudostratified epithelium. From 22nd week, the lining cells were composed of pseudostratified columnar cells. Whereas round cells disa, pp.ared gradually. Enlargement of lumen and pooling of sperms in ductus deferens coincided with the maturation of seminiferous tubules. 3. In simple correlation between the values of testis weight and the values from various measurements in the ductus deferens, there was significant correlation coefficient with each other. 4. In the India ink absorption test, India ink granules were not absorbed on the epithelium of the ductus deferens, but the granules reactive to acid phosphatase a, pp.ared in a line on the free border of each parts of the ductus deferens. The granules reactive to alkaline phosphatase were noted on the luminal border of ductus deferens mainly, but weak reaction showed than acid phophatase were a, pp.ared. The granules reactive to PAS were a, pp.ared mostly near on the free border of hte epithelial cells of ductus deferens. 5. Number of sperm, Indes of sperm vitality and MRT in the different parts of ductus deferens were tended to be somewhat dominant in the mid and lower parts than in u, pp.r part, even though not significant in the statistical analysis. Ratio of sperm abnormality was tended to be relatively high in the u, pp.r part too, and in the sperm of abnormality blunted head was less in number significantly in the mid and lower part than in the u, pp.r part.

• 한국환경생태학회지
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• 제25권6호
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• pp.861-866
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• 2011

우리는 제주도 대정읍에서 뼈나이테법(skeletochronology)을 사용하여 맹꽁이(Kaloula borealis)의 나이구조를 알아보았다. 조사는 번식기인 2009년 6월에 실시하였다. 측정된 개체수는 수컷이 38개체, 암컷이 58개체로 각 개체의 SVL(snout-vent length)과 체중을 측정하고 나이구조를 분석하였다. 수컷의 평균 SVL은 $43.69{\pm}3.44 mm$이며, 암컷은 $46.32{\pm}4.23mm$로 나타나고 있다. 첫 번식에 참여하는 수컷은 2살이고, 암컷은 3살로 나타났다. 가장 나이든 수컷은 8살이며 암컷은 10살이다. 그러므로 이 종의 수명은 수컷에서는 8살, 암컷에서는 10살 이상으로 볼 수 있으며 암수의 수명에서 암컷이 더 오래살고 있다. 또한 이 값은 통계적으로도 유의미하게 나타나고 있다. 성장곡선은 von Bertalanffy growth model을 사용하였으며, 성장계수(K)는 수컷이 0.56이며 암컷은 0.41이다. 최대성장크기는 수컷이 46.41 mm이며 암컷은 50.22 mm로 나타났다.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.161-167
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• 2010

Early diagnoses of pregnancy for animal such as swine and bovine is extremely important to increase income of a farmhouse and for the management of farm. For the development of immunoasaay system of pregnancy in swine, we report a competitive heterologous enzyme linked immunosorbent assay (ELISA) for the direct measurement of oestrone sulfate (E1S) in diluted urine using anti-E1G (glucuronide) monoclonal antibody which cross react with ElS. The principle of assay was based on the typical solid-phase competitive ELISA methods using E1G-HRP (horseradish peroxidase) as a tracer and E1S for standard. The method had a reasonable sensitivity for the detection of E1S with 0.15 ng/ml as a detection limit. The intra-assay and inter-assay precisions were raging coefficient of from 8.50~9.67% and 8.50~9.87%, respectively, which were quite acceptable. In a field trial with a group 37 sows (18 non-pregnancy and 19 pregnancy sows) after day 29~30 post service, the concentration of E1S were determined to be below 30 ng/ml in all non-pregnancy group and over 48 ng/ml in pregnancy group except one sample. The method described here, heterologous ELISA for the measurement of E1S in urine is good enough for monitoring the early pregnancy test of swine.

• 한국전기전자재료학회논문지
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• 제22권11호
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• pp.941-948
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• 2009

The present study aims at the examination of the effects of 1 mol% NiO addition on the reaction, microstructure development, resultant electrical properties, and especially the bulk trap and interface state levels of $ZnO-Bi_2O_3-Sb_2O_3$ (Sb/Bi=0.5, 1.0, and 2.0) systems (ZBS). The samples were prepared by conventional ceramic process, and characterized by density, XRD, SEM, I-V, impedance and modulus spectroscopy (IS & MS) measurement. The sintering and electrical properties of Ni-doped ZBS (ZBSN) systems were controlled by Sb/Bi ratio. Pyrochlore ($Zn_2Bi_3Sb_3O_{14}$) was decomposed more than $100^{\circ}C$ lowered in ZBS (Sb/Bi=1.0) by Ni doping. The reproduction of pyrochlore was suppressed by the addition of Ni in ZBS. Between two polymorphs of $Zn_7Sb_2O_{12}$ spinel ($\alpha$ and $\beta$), microstructure of ZBSN (Sb/Bi=0.5) composed of a-spinel was more homogeneous than $Sb/Bi{\geq}1.0$ composed of $\beta$-spinel phase. In ZBSN, the varistor characteristics were not improved drastically (non-linear coefficient $\alpha\;=\;6{\sim}11$) and independent on microstructure according to Sb/Bi ratio. Doping of Ni to ZBS seemed to form ${V_0}^{\cdot}$ (0.33 eV) as dominant bulk defect. From IS & MS, especially the grain boundaries of Sb/Bi=0.5 systems were divided into two types, i.e. sensitive to oxygen and thus electrically active one and electrically inactive intergranular one with temperature.

• 한국발생생물학회지:발생과생식
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• 제14권4호
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• pp.261-268
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• 2010

As in the O. mykiss electrophoretic profiles of RNA, the signals of each RNA sample from 9 individual tissues such as liver, muscle, brain, heart, pituitary gland, kidney, intestine, spleen and gill similar to positive control were obtained. The tissue distributions of the complimentary DNA (cDNA) of O. mykiss four genes were analyzed using quantitative real-time PCR with primer sets for tissue expression analysis. In this rainbow trout species, author obtained bands of various sizes, ranged from 700 bp to 1,400 bp. A dissociation curve was made at the end of each run to make sure that there was no non-specific amplification. Supplementarily, the Ct of each DNA was compared. The Ct values of vinculin with rainbow trout tissues were determined in a manner similar to those for agouti-related protein (AgRP) and melanocortin receptors (MC4R I and MC4R II). Further, obtained Cts for standard curve of each DNA were affected by specific product (vinculin, AgRP and MC4R II genes). After several experiments with four individual genes of rainbow trout, author estimated a variation ratio of the mean Ct value of the DNA extracted using the comparative CTt method was 37.27, and the standard deviation was 5.33. The correlation coefficient between the Ct values and the concentration of cDNA was -0.98, -0.99, -0.91 and -0.86, respectively (vinculin, AgRP, MC4R I and MC4R II genes). Since this correlation showed high linearity, the straight line obtained was used as a standard for the O. mykiss tissues reared in aquarium. A PCR efficiency of 100% is ideally achieved when the slopes are close to the theoretical value of -3.31. According to quantification method, the results of quantification are strongly affected by the DNA fragmentation. The size of most DNA fragments obtained from various tissues of rainbow trout used in the experiment was approximately 100 bp. According to the four slopes, an efficiency of nearly 100% was estimated for four genes detection methods. Additionally, further analysis with more individuals and primers will be required to fully establish optimization in rainbow trout.

• Reproductive and Developmental Biology
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• 제32권2호
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• pp.127-133
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• 2008

The objective of this study was two folds: to investigate the relationship between paternal identification rate and sperm quality parameters such as motility and sperm chromatin structure assay after heterospermic insemination; to see if mutual complement between tests and development of useful technique to enhance the fertility in artificial insemination. In individual boar's fertilizing ability, 3 high fertility boars showed significantly high fertility (p<0.05) compared to 3 low fertility boars, but there was no difference in litter size between two groups. Sperm motility test in pooled and individual semen using computer assisted sperm analysis (CASA) revealed that no significant difference among boars. The high fertile boar showed tendency of low %Red (High red fluorescence/green+red fluorescence) in sperm chromatin structure assay (SCSA) but paternal identification rate from piglets did not differ after heterospermic insemination. The correlation coefficient between individual or pooled semen function test and farrowing rates were well correlated as follows: %Red with litter size (r= - 0.53, p=0.03); %Red with paternal identification rates (r=-0.51, p=0.03); paternal identification rates with litter size (r=0.57, p=0.02). These results indicate that sperm chromatin structure assay and sperm quality parameter test in pooled semen are useful method to predict and evaluate the fertilizing capacity after heterospermic insemination in boars.

• Clinical and Experimental Reproductive Medicine
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• 제41권2호
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• pp.75-79
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• 2014

Objective: To study the effect of body composition on reproduction in women with unexplained infertility treated with a controlled ovarian hyperstimulation and intrauterine insemination programme. Methods: This prospective observational study was conducted on 308 unexplained infertile women who were scheduled for a controlled ovarian hyperstimulation and intrauterine insemination programme and were grouped as pregnant and non-pregnant. Anthropometric measurements were performed using TANITA-420MA before the treatment cycle. Body composition was determined using a bioelectrical impedance analysis system. Results: Body fat mass was significantly lower in pregnant women than in non-pregnant women ($15.61{\pm}3.65$ vs. $18.78{\pm}5.97$, respectively) (p=0.01). In a multiple regression analysis, body fat mass proved to have a stronger association with fecundity than the percentage of body fat, body mass index, or the waist/hip ratio (standardized regression coefficient${\geq}0.277$, t-value ${\geq}2.537$; p<0.05). The cut-off value of fat mass, which was evaluated using the receiver operating characteristics curve, was 16.65 with a sensitivity of 61.8% and a specificity of 70.2%. Below this cut-off value, the odds of the pregnancy occurrence was found to be 2.5 times more likely. Conclusion: Body fat mass can be predictive for pregnancy in patients with unexplained infertility scheduled for a controlled ovarian hyperstimulation and intrauterine insemination programme.