• Reproductive and Developmental Biology
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• 제32권3호
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• pp.159-166
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• 2008

본 연구는 vesicular stomatitis virus G glycoprotein (VSV-G)으로 피막이 형성되는 replication-defective MoMLV-based vector를 이용한 hTPO 헝질전환 닭의 생산에 관한 연구이다. 실험에 사용한 retrovirus vector의 구조는 hTPO 유전자의 발현 조절을 위해 internal promoter인 hCMV promoter를 이용하였으며 외래 유전자의 발현을 증가시키기 위해 woodchurk hepatitis virus posttranascriptional regulatory element (WPRE) 서열을 도입하였다. 재조합한 vector는 GP2 293 포장세포에 도입하여 virus를 생산하였으며 이 virus를 이용하여 감염시킨 여러 표적세포에서 hTPO의 발현과 생물학적 활성을 확인하였다. 재조합 hTPO의 생물학적 활성은 시판되고 있는 재조합 hTPO에 비해 우월한 것으로 확인되었다. hTPO 형질전환 닭의 생산을 위하여 1,000배 이상 고농도로 농축된 virus를 stage X 단계의 계란의 배반엽 층에 미세주입하여 대리난각 방법으로 배양하였다. 미세주입한 132개의 계란 중 21일 후에 11개의 계란에서 병아리가 부화하였으며 그중 4마리가 형질전환 개체로 확인되었다. 그러나 생산된 4마리 중 3마리가 부화 후 1개월 이내에 원인불명으로 사망하였다. 본 연구의 의의는 상업적 이용 가능성이 있는 생물학적 활성을 가진 사람의 cytokine 단백질의 대량 생산을 위한 생체 반응기로서의 형질전환 닭 개발의 시례를 제공하는데 있다.

• Reproductive and Developmental Biology
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• 제33권3호
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• pp.171-177
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• 2009

Spermatogonial stem cells(SSCs) only are responsible for the generation of progeny and for the transmission of genetic information to the next generation in male. Other in vitro studies have cultured SSCs for proliferation, differentiation, and genetic modification in mouse and rat. Currently, information regarding in vitro culture of porcine Germline Stem Cell(GSC) such as gonocyte or SSC is limited and is in need of further studies. Therefore, in this study, we report development of a successful culture system for gonocytes of neonatal porcine testes. Testis cells were extracted from $10{\sim}14$-day-old pigs. These cells were harvested using enzymatic digestion, and the harvested cells were purified with combination of percoll, laminin, and gelatin selection techniques. The most effective culture system of porcine gonocytes was established through trial experiments which made a comparison between different feeder cells, medium, serum concentrations, temperatures, and $O_2$ tensions. Taken together, the optimal condition was established using C166 or Mouse Embryonic Fibroblast(MEF) feeder cell, Rat Serum Free Medium(RSFM), 0% serum concentration, $37^{\circ}C$ temperature, and $O_2$ 20% tension. Although we discovered the optimal culture condition for proliferation of porcine gonocytes, the gonocyte colonies ceased to expand after one month. These results suggest inadequate acquirement of ingredients essential for long term culture of porcine GSCs. Consequently, further study should be conducted to establish a successful long-term culture system for porcine GSCs by introducing various growth factors or nutrients.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2007년도 추계학술대회 논문집
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• pp.429-429
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• 2007

For various applications of liquid crystal displays (LCDs), the uniform alignment of liquid crystal (LC) molecules on treated surfaces is significantly important. Generally, a rubbing method has been widely used to align the LC molecules on polyimide (PI) surfaces. Rubbed PI surfaces have suitable characteristics, such as uniform alignment. However, the rubbing method has some drawbacks, such as the generation of electrostatic charges and the creation of contaminating particles. Thus, we strongly recommend a non contact alignment technique for future generations of large high-resolution LCDs. Most recently, the LC aligning capabilities achieved by ultraviolet and ion-beam exposures which are non contact methods, on diamond-like carbon (DLC) inorganic thin film layers have been successfully studied because DLC thin films have a high mechanical hardness, a high electrical resistivity, optical transparency, and chemical inertness. In addition, nitrogen-doped DLC (NDLC) thin films exhibit properties similar to those of the DLC thin films and a higher thermal stability than the DLC thin films because C:N bonding in the NDLC thin filmsis stronger against thermal stress than C:H bonding in the DLC thin films. Our research group has already studied the NDLC thin films by an ion-beam alignment method. The $SiN_x$ thin films deposited by plasma-enhanced chemical vapor deposition are widely used as an insulation layer for a thin film transistor, which has characteristics similar to those of DLC inorganic thin films. Therefore, in this paper, we report on LC alignment effects and pretilt angle generation on a $SiN_x$, thin film treated by ion-beam irradiation for various N ratios

• 건축역사연구
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• 제28권2호
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• pp.77-90
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• 2019

The Sacheonwangsa temple in Silla was completed in 679, just after the unification of the Three Kingdoms. In recent years, we have been critically considering the history of the chronology based on the existence of 'Geumdang of the previous generation', which has emerged through the research report of the Sacheonwangsa temple. It is the one to reconsider the construction process of the Sacheonwangsa temple centering on the re-interpretation of the construction time and the character of the first stage of the foundation which was confirmed from within the Geumdang site. The results of this study are as follows : 1)The "Chang(創) of Sacheonwangsa temple" in [Three Affairs that Queen Seondeok had already known] that it is presumed that the construction of the Sacheonwangsa temple, which was designed by Anham before 640 years ago, will convey the fact that the first of the King Munmu's reign (661 ~) has been finalized after the initial discussion. 2)Although the theories after excavations are predicated on the existence of 'Geumdang of the previous generation', there is no reason to believe that a lasting predecessor building with roof and pillar walls on predecessor buildings is considered to have been built. The foundation was associated with the "build a temple out of coloured silk(以彩帛營寺)" i.e. 'Jochang(祖創)' in 670 years before the construction plan was formally finalized. However, it is presumed that the remains of the platform construction on the premise that it will be used on the construction of Geumdang. 3)The decision to 'rebuilding( ??)' based on a formal construction plan is determined to be from 670 to 672 years. The maintaining of the original cathedral axis line, to the north on the boundary of the southern limit of the foundation flat portion, the result of developing and embodied the relative position of the Geumdang in the newly determined cathedral unfold and embody, the center of the building base and Geumdang, it is presumed that the centers of them are divided into North and south. 4)The completion of the Sacheonwangsa temple in 679 is understood as the conclusion of 'rebuilding' to "build a temple out of coloured silk" i.e. 'Jochang' in 670 years. The pent roof of the Geumdang was initially considered not to be in the plan, but it is judged to be before the completion of the Sacheonwangsa temple.

• 한국차세대컴퓨팅학회논문지
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• 제13권2호
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• pp.18-25
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• 2017

인코딩(encoding)은 컴퓨터에서 사용되는 음성, 영상 및 텍스트 문자를 부호화하는 방법을 가리킨다. 그러므로 특정 데이터를 열람하기 위해서는 해당 인코딩 정보를 알아야하며, 데이터마다 인코딩을 판별해주는 알고리즘들이 존재한다. 하지만 실제 음원이나 문서를 송수신하는 과정에서 패킷 손실이 발생할 수 있으며, 특히 무선 통신망에서 패킷 스니핑으로 정보를 가로챌 경우 손실률은 더욱 증가되어 인코딩 기법 판별에 어려움이 발생한다. 본 논문에서는 이러한 오류가 발생한 데이터의 인코딩 기법 판별율을 향상시키기 위해 기존의 문자 인코딩 기법 판별 프로그램인 'uchardet'에 Bit-shift 알고리즘을 적용하여 성능 향상을 이루었다. 알고리즘의 성능 평가를 위해 임의의 한글 및 일본어 텍스트 파일에 손실률(loss rate)을 적용하여 부분적으로 데이터가 소실된 인코딩 파일을 생성하여 결과를 비교하였다. 그 결과, 패킷이 손실된 데이터에서 Bit-shift 알고리즘을 적용한 'uchardet-bitshift' 경우 기존의 알고리즘보다 더 나은 성능을 보였다. 한국어 인코딩의 경우 기존의 uchardet는 0.005% 손실률까지 100%의 정확도를 보이고 1%보다 높은 손실률에서는 인코딩을 전혀 판별해 내지 못한데 비해, Bit-shift 알고리즘을 적용할 경우 0.05%의 손실률에도 100%의 정확도를 보였으며 그보다 큰 손실률에서도 해당 인코딩을 판별해냈다. 또한 한자어를 많이 포함하는 일본어의 경우 손실률이 높아질수록 중국어 인코딩으로 잘못 판별하는 경향을 보였다. 시뮬레이션 분석 결과, Bit shift 알고리즘을 추가하여 기존 인코딩 기법 판별 알고리즘의 개선이 가능하였다.

• Journal of Genetic Medicine
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• 제16권2호
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• pp.67-70
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• 2019

Limb-girdle muscular dystrophy (LGMD) is a group of muscular dystrophies that has extremely heterogeneous clinical features and genetic background. The caveolin-3 gene (CAV3) is one of the causative genes. LGMD appears as a clinical continuum, from isolated skeletal muscle involvement to long QT syndrome. Here we report two patients without apparent muscle weakness in a family with CAV3 mutation. A 7-month-old Korean boy visited our muscle clinic because of an incidental finding of elevated serum creatine kinase (CK) concentration (680 IU/L, reference range, 20-270 IU/L) without clinical symptoms. The patient was born after an uneventful pregnancy and showed normal developmental milestones. He developed pseudohypertrophy of his calf muscle during the follow-up. We obtained a muscle biopsy at age 14 months, which showed size variations and degenerating/regenerating myofibers with endomysial fibrosis and immunohistochemical evidence of normal dystrophin. Under the impression of LGMD, we performed target panel sequencing and identified a heterozygous in-frame mutation of CAV3, c.307_312delGTGGTG (p.Val103_Val104del). Immunohistochemical staining of muscle indicated complete loss of caveolin-3 compared with normal control muscle, which supported the variant's pathogenicity. We performed segregation analysis and found that the patient's mother had the same variant with elevated serum CK level (972 IU/L). We report on autosomal dominant familial caveolinopathy caused by a pathogenic variant in CAV3, which was asymptomatic until the fourth decade. This case highlights the utility of next generation sequencing in the diagnosis of muscular dystrophies and the additive role of muscle biopsy to confirm the variants.

• Journal of Korean Neurosurgical Society
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• 제29권8호
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• pp.1024-1029
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• 2000

Objectives : With recent improvements in neuroimaging and the development of third and fourth-generation radiosurgical dose-planning soft ware, came a renewed interest in using radiosurgery for the treatment of movement disorders. Radiosurgery involves no opening of the cranium and no incisions, eliminating both the risk of hemorrhage from passing an electrode to the depths of meningitis from operative infection. It is for these reasons stereotactic radiosurgical treatment of movement disorders has value in a small subgroup of patients. The authors report four cases of Parkinson's disease and one case of dystonia that were treated by Gamma knife. Methods : Radiosurgical nucleus ventralis intermedius thalamotomy using the gamma knife unit was performed to make lesion in two Parkinson's disease patients. A radiation dose of 120Gy was delivered to nucleus using a single 4-mm collimator plug pattern following classic anatomical landmarks. Patients were followed for a median of 10.5 months(range 9-12 months). An independent neurological evaluation of tremor, based on the change in the United Parkinson's Disease Rating Scale tremor score(UPDRS), was correlated with a subjective evaluation. Gamma knife ventrolateral(V.O.P) thalamotomy was performed in one case of dystonia. A central dose of 150Gy was delivered and the patient was followed for 18 months. Gamma knife globus pallidus interna pallidotomy was performed in two Parkinson's disease patients. A radiation dose of 130Gy(range 120-140Gy) was delivered. Patients were followed for a median of 13 months(range 9-14 months). Result : Ventrolateral thalamotomy in dystonia produced regained left hand usage in order to be able to use the telephone. Ventralis intermedius thalamotomy produced an excellent improvement of the tremor in one case, mild improvement of the tremor in the other case of Parkinson's disease. A globus pallidus internalis(GPi) pallidotomy produced improvement of rigidity and dyskinesia : one other showed no change. There were no neurological complications. Conclusion : Gamma Knife thalamotomy considered a safe and effective technique for the treatment of tremor in Parkinson's disease. Although the results from Longer follow-up is not available yet, the short-term results seem to be encouraging.

• 해양환경안전학회지
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• 제22권1호
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• pp.108-117
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• 2016

본 논문에서는 MRV 규제 대응을 위해 선박 운항효율데이터 수집 및 검증 시스템을 구축하고 이를 활용하여 해운선사 업무지원과 국가 온실가스 인벤토리 산정이 가능한 국제해운 에너지효율 포탈 시스템을 설계하였다. 시스템 요구조건을 도출하기 위하여 EU MRV 법안과 IMO MRV 논의사항, 해운선사의 선박에너지효율과 온실가스규제 대응 및 국제해운 온실가스 통계 현황에 대하여 분석하고 선박 에너지효율과 온실가스 배출량 데이터 수집을 위하여 선내 장비, 에너지효율 측정 장비, 운항보고서를 활용한 데이터 수집 모듈과 선박에서 수집된 데이터를 육상으로 전송하기 위해 관리가 용이하고 사용량이 최소화된 데이터베이스기반 전송 모듈을 설계하였다. 수집된 데이터의 표준보고양식 변환, 모니터링, 통계 및 분석, 검증, 보고서 자동생성과 국가 온실가스 인벤토리 지원이 가능한 국제해운 에너지 포탈시스템을 설계하였다.

• 천문학회보
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• 제42권1호
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• pp.40.1-40.1
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• 2017

The NISS (Near-infrared Imaging Spectrometer for Star formation history) is the near-infrared spectro-photometric instrument optimized to the Next Generation of small satellite series (NEXTSat). To achieve the major scientific objectives for the study of the cosmic star formation in local and distant universe, the spectro-photometric survey covering more than 100 square degree will be performed. The main observational targets will be nearby galaxies, galaxy clusters, star-forming regions and low background regions. The off-axis optics was developed to cover a wide field of view ($2deg.{\times}2deg.$) as well as the wide wavelength range from 0.95 to $2.5{\mu}m$, which were revised based upon the recent test and evaluation of the NISS instrument. The mechanical structure were tested under the launching condition as well as the space environment. The signal processing from infrared sensor and the communication with the satellite were evaluated after the integration into the satellite. The flight model of the NSS was assembled and integrated into the satellite. To verify operations of the satellite in space, the space environment tests such as the vibration, shock and thermal-vacuum test were performed. The accurate calibration data were obtained in our test facilities. Here, we report the test results of the flight model of the NISS.

• 한국전기전자재료학회논문지
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• 제32권3호
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• pp.201-206
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• 2019

This report constitutes the first demonstration in Korea of single-crystal lateral gallium oxide ($Ga_2O_3$) as a metal-oxide-semiconductor field-effect-transistor (MOSFET), with a breakdown voltage in excess of 480 V. A Si-doped channel layer was grown on a Fe-doped semi-insulating ${\beta}-Ga_2O_3$ (010) substrate by molecular beam epitaxy. The single-crystal substrate was grown by the edge-defined film-fed growth method and wafered to a size of $10{\times}15mm^2$. Although we fabricated several types of power devices using the same process, we only report the characterization of a finger-type MOSFET with a gate length ($L_g$) of $2{\mu}m$ and a gate-drain spacing ($L_{gd}$) of $5{\mu}m$. The MOSFET showed a favorable drain current modulation according to the gate voltage swing. A complete drain current pinch-off feature was also obtained for $V_{gs}<-6V$, and the three-terminal off-state breakdown voltage was over 482 V in a $L_{gd}=5{\mu}m$ device measured in Fluorinert ambient at $V_{gs}=-10V$. A low drain leakage current of 4.7 nA at the off-state led to a high on/off drain current ratio of approximately $5.3{\times}10^5$. These device characteristics indicate the promising potential of $Ga_2O_3$-based electrical devices for next-generation high-power device applications, such as electrical autonomous vehicles, railroads, photovoltaics, renewable energy, and industry.