• Journal of Information Processing Systems
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• v.9 no.2
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• pp.205-216
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• 2013

In this paper, we analyze a technique for building a high-availability (HA) cluster system. We propose what we have termed the 'Selective Replication Manager (SRM),' which improves the throughput performance and reduces the latency of disk devices by means of a Distributed Replicated Block Device (DRBD), which is integrated in the recent Linux Kernel (version 2.6.33 or higher) and that still provides HA and failover capabilities. The proposed technique can be applied to any disk replication and database system with little customization and with a reasonably low performance overhead. We demonstrate that this approach using SRM increases the disk replication speed and reduces latency by 17% and 7%, respectively, as compared to the existing DRBD solution. This approach represents a good effort to increase HA with a minimum amount of risk and cost in terms of commodity hardware.

• Journal of Veterinary Science
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• v.25 no.2
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• pp.21.1-21.15
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• 2024

Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.

• YAKHAK HOEJI
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• v.36 no.5
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• pp.449-454
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• 1992

The effect of radioprotective ginseng protein fraction on DNA repair capacity was determined by measuring the amount of $^{3}H-thymidine$ incorporated into DNA in the process of repair synthesis for UV damaged DNA. CHO-Kl cells were prepared whose semiconservative replication was inhibited by trimethylpsoralen plus near-UV(PUVA) treatment. When the cells were exposed to UV light alone, the DNA repair capacity was increased at first and then decreased as UV dose increased. However, when the ginseng fraction was treated to the cells, the DNA repair capacity was kept increasing regardless of UV dose increment. When the concentration of protein contained in the added fraction was increased gradually, the repair capacity was also increased almost linearly showing dose-response relationship of the effect. These results suggest that the enhancement of DNA repair capacity of the cell can be one of the mechanisms of radioprotection by the ginseng fraction.

• The Journal of Korean Association of Computer Education
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• v.12 no.4
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• pp.65-75
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• 2009

The result verification is necessary to support a guarantee for the correctness of the task results be executed by any unspecified resources in desktop grid environments. Typically, voting-based and trust-based result verification schemes have been used in the environments. However, these suffer from two potential problems: waste of resources due to redundant replicas of each task and increase in turnaround time due to the inability to deal with a dynamic changeable execution environment. To overcome these problems, we propose a fuzzy inference-based replication scheme which can adaptively determine the number of replicas per task by using both trusty degree and result return probability of resources. Therefore our proposal can reduce waste of resources by determining the number of replicas meeting with a dynamic execution environment of desktop grids, not to mention an enhancement of turnaround time for entire asks. Simulation results show that our scheme is superior to other ones in terms of turnaround time, the waste of resources, and the number of re-replications per task.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.2
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• pp.137-141
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• 2002

An experimental study was undertaken to quantify the effects of insect hormones on the replication of nucleopolyhedrovirus (NPV). The results demonstrated that TCID/ sub 50/ at 72 h post-infection (hpi) rose systematically from 0.55$\times$10$^{8}$ /m1, for untreated cells, up to 1.67$\times$10$^{8}$ / ml at 3$\mu$g/ml, then dropped down to 1.45$\times$10$^{8}$ /m1 at 4 $\mu$g/ml, by adding ecdysone to the culture medium for Bm-N cells infected with a wild-type Bambyx mori. nucleopolyhedrovirus (BmNPV). The optimum enhancement of about 3 times on budded virus (BV) titer at 72 hpi was given at 3 $\mu$g/ml of ecdysone. While the polyhedra number had no obvious variation within the range of concentrations from 0 to 4 $\mu$g/ml. By addition of juvenile hormone analogue (JHA) into the media with this concentration range, the BmNPV TCID/ sub 50/ and polyhedra number at 72 hpi did not show significant changes. Also, the addition of either 3 $\mu$g/ml of ecdysone or 3 $\mu$g/ml of JHA to the culture media did not appear to affect the TCID/ sub 50/ and polyhedra number significantly in infected Sf-21 cells with the autographa californica nucleopolyhedrovirus (AcMNPV).

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.8 no.5
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• pp.187-195
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• 2008

In MANET, it is important to efficiently manage data objects and traffic to improve network throughput and response time. This paper proposes a connection-degree based data object search and replication scheme in mobile ad-hoc networks. In this method, information about the path data object providers is stored at the cache of a cluster header at which lots of queries arrive, so that, to reduce the load of the cluster header, replicas can be created at its neighboring nodes. The method proposes an algorithm that picks up a cluster header among mobile nodes and makes it possible to search for and utilize adaptive and up-to-date information in MANET. The method is expected to be effective since it enables access to data objects in spite of broken links among mobile nodes with an enhancement in network response time of searching and a decrease in communication costs. The efficiency of this system was verified via simulation.

• Proceedings of the KSPS conference
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• 2003.10a
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• pp.101-104
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• 2003

In this paper, speech quality is improved by removing abrupt noise intervals and then substituting the gaps with estimates of the previous speech waveform. An abrupt noise detection signal has been proposed as a prediction error signal by utilizing LP coefficients of the previous frame. Abrupt noise intervals are estimated by using spectral energy. After removing estimated noise intervals, we applied several waveform substitution techniques such as zero substitution, previous frame repetition, pattern matching, and pitch waveform replication. To prove the validity of our algorithm, the LPC spectral distortion test and the recognition test are executed and, the results show that the speech quality is fairly well improved.

• International Journal of Oral Biology
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• v.33 no.1
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• pp.25-31
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• 2008

Inhibition of proteasome activity may reduce many types of cancer, so it's pathway is effective in cancer as well as in clinical fields. Here the author has carried out experiment targeting on the elevation of apoptosis in oral cancer cells by combination of proteasome inhibitor, lactacystin, and DNA replication inhibitor, etoposide. The growth of KB cells was measured by MTT methods and apoptosis was analyzed by DNA fragmentation and Hochest nucleus staining. The proteasome activity was analyzed by fluorescent tagged peptide and cellular protein expression was detected by Western hybridization. Though lactacystin and etoposide inhibited KB cell growth alone, but low combined doses inhibited cell growth more strongly and induced apoptosis. The proteasome activity was also seriously inhibited by the combination of both chemicals. Tumor suppressor proteins and apoptosis inducing proteins were highly increased under the combination of both chemicals. From above studies we can conclude that proteasome inhibitors may be used for the treatment of oral cancer and proteasome inhibitors with DNA replication inhibitors may be effective in clinical trials of oral cancer.

• Design & Manufacturing
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• v.2 no.2
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• pp.29-32
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• 2008

A back light unit (BLU) is a key module of a thin film transistor liquid crystal display (TFT-LCD), frequently utilized in various mobile displays. In this study, we experimentally characterize transcription and optical properties of concave and convex microlens arrays (MLAs) of light guide plate (LGP) fabricated by injection molding with polycarbonate as a LGP substrate material. Nickel mold inserts were manufactured by electroforming on the MLA which was fabricated by the thermal reflow of photoresist microstructures patterned by UV-photolithography. For the case of convex microlens, the height of replicated microlens was less than that of the mold insert while maintaining almost the same microlens diameter of the mold insert as the location of the microlens is far from the gate. In contrast, for the concave microlens, the diameter of replicated microlens was larger than that of mold insert, while showing almost the same microlens height as the mold insert. From the optical examination of replicated convex and concave MLAs, it was found that a higher luminance of the LGP was achieved by the concave MLAs compared to the convex MLAs (about 30% enhancement in this case)due to the utilization of a larger amount of light provided by the light sources.

• Journal of KIISE:Software and Applications
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• v.37 no.7
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• pp.503-517
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• 2010

Service-oriented computing, the effective paradigm for developing service applications by using reusable services, becomes popular. In service-oriented computing, service consumer has no responsibility for managing services, just invokes services what service providers are producing. On the other hand, service providers should manage any resources and data for service consumers can use the service anytime and anywhere. However, it is hard service providers manage the quality of the services because an unspecified number of service consumers. Therefore, service scalability for providing services with higher quality of services specified in a service level agreement becomes a potential problem in service-oriented computing. There have been many researches for scalability in network, database, and distributed computing area. But a research about a definition of service scalability and metrics of measuring service scalability is still not mature in service engineering area. In this paper, we construct a service network which connects multiple service nodes, and integrate all the resources to manage it. And we also present a service scalability framework for managing service scalability by using a mechanism of service migration or replication. In section 3, we, firstly, present the structure of the scalability management framework and basic functionalities. In section 4, we propose scalability enhancement mechanism which is needed to release functionality of the framework. In section 5, we design and implement the framework by using proposed mechanism. In section 6, we demonstrate the result of our case study which dynamically manages services in multi-nodes environment by applying our framework. Through the case study, we show the applicability of our scalability management framework and mechanism.