International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Effects of Insect Hormones on the Replication of Nucleopolyhedrovirus

  • Zhang, Zhi-Fang (Key Laboratory of Silkworn Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of agric) ;
  • Yi, Yong-Zhu (Key Laboratory of Silkworn Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of agric) ;
  • Xiao, Qing-Li (Key Laboratory of Silkworn Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of agric) ;
  • He, Jia-Lu (Key Laboratory of Silkworn Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of agric) ;
  • Zhou, Ya-Jing (Key Laboratory of Silkworn Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of agric) ;
  • Zhang, Yuan-Xing (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 2000237, P. R.)
  • Published : 2002.06.01
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Abstract

An experimental study was undertaken to quantify the effects of insect hormones on the replication of nucleopolyhedrovirus (NPV). The results demonstrated that TCID/ sub 50/ at 72 h post-infection (hpi) rose systematically from 0.55$\times$10$^{8}$ /m1, for untreated cells, up to 1.67$\times$10$^{8}$ / ml at 3$\mu$g/ml, then dropped down to 1.45$\times$10$^{8}$ /m1 at 4 $\mu$g/ml, by adding ecdysone to the culture medium for Bm-N cells infected with a wild-type Bambyx mori. nucleopolyhedrovirus (BmNPV). The optimum enhancement of about 3 times on budded virus (BV) titer at 72 hpi was given at 3 $\mu$g/ml of ecdysone. While the polyhedra number had no obvious variation within the range of concentrations from 0 to 4 $\mu$g/ml. By addition of juvenile hormone analogue (JHA) into the media with this concentration range, the BmNPV TCID/ sub 50/ and polyhedra number at 72 hpi did not show significant changes. Also, the addition of either 3 $\mu$g/ml of ecdysone or 3 $\mu$g/ml of JHA to the culture media did not appear to affect the TCID/ sub 50/ and polyhedra number significantly in infected Sf-21 cells with the autographa californica nucleopolyhedrovirus (AcMNPV).

Keywords

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