• Journal of Information Processing Systems
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• 제9권2호
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• pp.205-216
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• 2013

In this paper, we analyze a technique for building a high-availability (HA) cluster system. We propose what we have termed the 'Selective Replication Manager (SRM),' which improves the throughput performance and reduces the latency of disk devices by means of a Distributed Replicated Block Device (DRBD), which is integrated in the recent Linux Kernel (version 2.6.33 or higher) and that still provides HA and failover capabilities. The proposed technique can be applied to any disk replication and database system with little customization and with a reasonably low performance overhead. We demonstrate that this approach using SRM increases the disk replication speed and reduces latency by 17% and 7%, respectively, as compared to the existing DRBD solution. This approach represents a good effort to increase HA with a minimum amount of risk and cost in terms of commodity hardware.

• Journal of Veterinary Science
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• 제25권2호
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• pp.21.1-21.15
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• 2024

Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.

• 약학회지
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• 제36권5호
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• pp.449-454
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• 1992

The effect of radioprotective ginseng protein fraction on DNA repair capacity was determined by measuring the amount of $^{3}H-thymidine$ incorporated into DNA in the process of repair synthesis for UV damaged DNA. CHO-Kl cells were prepared whose semiconservative replication was inhibited by trimethylpsoralen plus near-UV(PUVA) treatment. When the cells were exposed to UV light alone, the DNA repair capacity was increased at first and then decreased as UV dose increased. However, when the ginseng fraction was treated to the cells, the DNA repair capacity was kept increasing regardless of UV dose increment. When the concentration of protein contained in the added fraction was increased gradually, the repair capacity was also increased almost linearly showing dose-response relationship of the effect. These results suggest that the enhancement of DNA repair capacity of the cell can be one of the mechanisms of radioprotection by the ginseng fraction.

• 컴퓨터교육학회논문지
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• 제12권4호
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• pp.65-75
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• 2009

결과 검증은 데스크톱 그리드 환경에서 불특정 자원들에 의해 수행된 작업 결과의 정확성을 보장하는데 필수적이다. 전형적으로 투표기반 기법과 믿음기반의 기법이 데스크톱 그리드 환경에서 사용되어 왔지만, 이들 기법은 작업의 잉여 중복에 의한 자원 낭비와 동적 환경의 비적응성에 의한 반환 시간의 증가 문제를 갖고 있다. 이러한 문제점을 해결하기 위해 본 논문에서는 자원의 믿음 정도와 결과 반환 확률을 이용하여 적응적으로 작업 복제 개수를 결정할 수 있는 퍼지 추론 기반의 복제 기법을 제안한다. 따라서, 제안 기법은 동적 연산 환경의 적절한 대처를 통해 작업 복제 개수를 결정함으로써 자원 낭비를 줄여주고 전체 작업의 반환 시간을 향상시킨다. 시뮬레이션 결과는 제안 기법이 반환 시간, 자원 낭비, 작업의 재복제 횟수의 관점에서 우수함을 보여준다.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.137-141
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• 2002

An experimental study was undertaken to quantify the effects of insect hormones on the replication of nucleopolyhedrovirus (NPV). The results demonstrated that TCID/ sub 50/ at 72 h post-infection (hpi) rose systematically from 0.55$\times$10$^{8}$ /m1, for untreated cells, up to 1.67$\times$10$^{8}$ / ml at 3$\mu$g/ml, then dropped down to 1.45$\times$10$^{8}$ /m1 at 4 $\mu$g/ml, by adding ecdysone to the culture medium for Bm-N cells infected with a wild-type Bambyx mori. nucleopolyhedrovirus (BmNPV). The optimum enhancement of about 3 times on budded virus (BV) titer at 72 hpi was given at 3 $\mu$g/ml of ecdysone. While the polyhedra number had no obvious variation within the range of concentrations from 0 to 4 $\mu$g/ml. By addition of juvenile hormone analogue (JHA) into the media with this concentration range, the BmNPV TCID/ sub 50/ and polyhedra number at 72 hpi did not show significant changes. Also, the addition of either 3 $\mu$g/ml of ecdysone or 3 $\mu$g/ml of JHA to the culture media did not appear to affect the TCID/ sub 50/ and polyhedra number significantly in infected Sf-21 cells with the autographa californica nucleopolyhedrovirus (AcMNPV).

• 한국인터넷방송통신학회논문지
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• 제8권5호
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• pp.187-195
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• 2008

이동 애브-혹 네트워크에서 이동 노드들이 자신이 원하는 정보를 찾고 이를 복제하는 연구들은 유비쿼터스 환경에서 아주 필요한 응용이다. 본 논문에서는 연결 차수를 기반으로 하여 파일, 컨텐츠등 데이터 제공자의 경로 정보를 로컬 캐쉬에 저장하고, 파일의 복제본을 최소화하여 생성하는 기법을 제안한다. 이 기법에서는 질의가 많이 도착하는 클러스터 헤더 노드에 파일 제공자의 경로 정보를 저장하고, 클러스터 헤더 노드의 부하를 줄이기 위하여 그 이웃노드에 복제본을 생성한다. 이는 제한적이고 지역적인 정보만을 이용하는 이동 노드들중 클러스터 헤더를 판별하는 알고리즘을 제시하며, 동적인 네트워크에서 확장적이고 적응적이며 최신의 정보를 검색하고 활용할 수 있도록 한다. 이는 검색의 정확성과 응답 시간이 빨라지고 네트워크 통신 비용을 줄이며, 이동 노드들간의 연결 단절 상황에서도 데이터를 액세스 할 수 있어 효과적이다. 이의 효율성을 시뮬레이션을 통하여 보인다.

• 대한음성학회:학술대회논문집
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• 대한음성학회 2003년도 10월 학술대회지
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• pp.101-104
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• 2003

In this paper, speech quality is improved by removing abrupt noise intervals and then substituting the gaps with estimates of the previous speech waveform. An abrupt noise detection signal has been proposed as a prediction error signal by utilizing LP coefficients of the previous frame. Abrupt noise intervals are estimated by using spectral energy. After removing estimated noise intervals, we applied several waveform substitution techniques such as zero substitution, previous frame repetition, pattern matching, and pitch waveform replication. To prove the validity of our algorithm, the LPC spectral distortion test and the recognition test are executed and, the results show that the speech quality is fairly well improved.

• International Journal of Oral Biology
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• 제33권1호
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• pp.25-31
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• 2008

Inhibition of proteasome activity may reduce many types of cancer, so it's pathway is effective in cancer as well as in clinical fields. Here the author has carried out experiment targeting on the elevation of apoptosis in oral cancer cells by combination of proteasome inhibitor, lactacystin, and DNA replication inhibitor, etoposide. The growth of KB cells was measured by MTT methods and apoptosis was analyzed by DNA fragmentation and Hochest nucleus staining. The proteasome activity was analyzed by fluorescent tagged peptide and cellular protein expression was detected by Western hybridization. Though lactacystin and etoposide inhibited KB cell growth alone, but low combined doses inhibited cell growth more strongly and induced apoptosis. The proteasome activity was also seriously inhibited by the combination of both chemicals. Tumor suppressor proteins and apoptosis inducing proteins were highly increased under the combination of both chemicals. From above studies we can conclude that proteasome inhibitors may be used for the treatment of oral cancer and proteasome inhibitors with DNA replication inhibitors may be effective in clinical trials of oral cancer.

• Design & Manufacturing
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• 제2권2호
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• pp.29-32
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• 2008

A back light unit (BLU) is a key module of a thin film transistor liquid crystal display (TFT-LCD), frequently utilized in various mobile displays. In this study, we experimentally characterize transcription and optical properties of concave and convex microlens arrays (MLAs) of light guide plate (LGP) fabricated by injection molding with polycarbonate as a LGP substrate material. Nickel mold inserts were manufactured by electroforming on the MLA which was fabricated by the thermal reflow of photoresist microstructures patterned by UV-photolithography. For the case of convex microlens, the height of replicated microlens was less than that of the mold insert while maintaining almost the same microlens diameter of the mold insert as the location of the microlens is far from the gate. In contrast, for the concave microlens, the diameter of replicated microlens was larger than that of mold insert, while showing almost the same microlens height as the mold insert. From the optical examination of replicated convex and concave MLAs, it was found that a higher luminance of the LGP was achieved by the concave MLAs compared to the convex MLAs (about 30% enhancement in this case)due to the utilization of a larger amount of light provided by the light sources.

• 한국정보과학회논문지:소프트웨어및응용
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• 제37권7호
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• pp.503-517
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• 2010

서비스 지향 컴퓨팅은 재사용 가능한 서비스를 사용하여 애플리케이션을 개발하는 효과적인 패러다임으로서 널리 각광받고 있다. 서비스 지향 컴퓨팅에서 서비스 소비자는 서비스를 관리하거나, 수정할 필요가 없이 서비스 제공자가 제공하는 서비스를 단지 사용하기만 하면 된다. 반면에, 서비스 제공자는 사용자가 원하는 때에 서비스를 사용할 수 있도록 자원 및 데이터 등을 관리하여야 한다. 하지만, 서비스 소비자는 불특정 다수의 특성을 갖기 때문에 서비스 제공자가 서비스 품질을 관리하기 힘들다. 따라서 서비스 수준 협약에 명시된 품질을 보장하면서 여러 명의 소비자에게 서비스를 제공하기 위한 서비스 확장성이 서비스 지향 컴퓨팅의 잠재적인 문제로 주목 받고 있다. 확장성에 대한 연구는 네트워크와 데이터베이스, 분산 컴퓨팅 등의 여러 분야에서 진행되었다. 하지만, 서비스 공학 분야에서는 아직 서비스 확장성의 정의와 관련 메트릭 등의 연구가 미흡한 실정이다. 본 논문에서는 서비스 환경을 다중 노드가 연결된 네트워크로 구성하고, 모든 노드의 자원을 통합 관리한다. 또한 동적인 서비스의 복제 및 이주 기법을 이용하여 서비스 확장성을 관리하기 위한 프레임워크를 제안한다. 3장에서는 확장성 관리 프레임워크의 구조와 기능성을 소개하고, 4장에서는 프레임워크의 기능성 실현에 필요한 확장성 향상 기법을 제안한다. 5장에서는 제안된 기법을 적용하여 프레임워크를 설계/구현하며, 6장에서는 구현된 프레임워크를 적용하여 실험을 수행한다. 실험을 통해 확장성 향상 기법의 실효성을 확인한다.