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Influence of Spirulina Level in Diet on Skin Color of Red- and White-colored Fancy Carp Cyprinus carpio var. koi (배합사료 내 Spirulina 함량에 따른 비단잉어 홍백 치어의 체색 변화)

  • Kim, Yi-Oh;Lee, Sang-Min
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.2
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    • pp.414-421
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    • 2015
  • This study was conducted to investigate the effects of Spirulina level in the diet on growth and skin color of red- and white-colored fancy carp Cyprinus carpio var. koi at different feeding periods. Five experimental diets (designated as Con, S5, S9, S13 and S17) were prepared to contain 0, 5, 9, 13 and 17% Spirulina. Each experimental diet was fed to two replicate groups of fish (6.6 g/fish) to visual satiation three times a day for 12 weeks. Weight gain and feed efficiency of fish were not affected by dietary Spirulina level. The values of redness ($a^*$), lightness ($L^*$) and yellowness ($b^*$) of the fish skin were influenced by dietary Spirulina level at different feeding period. The $L^*$ value of skin in the fish fed S9, S13 and S17 diets tended to decrease with feeding period. The skin $a^*$ value of the fish increased significantly with feeding period and dietary Spirulina level. The skin $a^*$ value of the fish fed the diets containing 5-17% Spirulina was higher than that of fish fed control diet at 6 and 12 weeks of feeding. The skin $a^*$ value and total carotenoids of the fish fed the S17 diet was the highest among groups. Based on the results of this study, it can be concluded that dietary inclusion of Spirulina powder could increase the skin redness of red- and white-colored fancy carp.

Lysine Requirement of Male White Pekin Ducklings from Seven to Twenty-one Days of Age

  • Xie, Ming;Guo, Yuming;Zhang, Ting;Hou, Shuisheng;Huang, Wei
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.10
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    • pp.1386-1390
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    • 2009
  • A dose-response experiment with five lysine levels (0.65, 0.80, 0.95, 1.10, and 1.25%) was conducted to evaluate the lysine requirement of male White Pekin ducklings from 7 to 21 days of age. Two hundred and eighty, 7-day-old, male White Pekin ducklings were allocated to 5 experimental treatments, each containing 8 replicate pens with 7 birds per pen. Feed and water were provided ad libitum from 7 to 21 days of age. At 21 days of age, weight gain, feed intake, feed/gain, breast meat weight, and breast meat yield relative to body weight of ducklings from each pen were all measured. As dietary lysine level increased, weight gain, feed intake, feed/gain, breast meat weight, and breast meat yield of ducklings were all improved significantly (p<0.05). According to broken-line regression analysis, the lysine requirement of male White Pekin ducklings from 7 to 21 days of age for weight gain, feed/gain, breast meat weight, and breast meat yield was 0.84, 0.90, 0.97, and 0.98%, respectively. Considering that Pekin duck production is directed to meat production, the lysine requirement of male starter Pekin ducklings during this period is suggested to be 0.98%.

Effect of Selenium-enriched Bean Sprout and Other Selenium Sources on Productivity and Selenium Concentration in Eggs of Laying Hens

  • Chinrasri, O.;Chantiratikul, P.;Thosaikham, W.;Atiwetin, P.;Chumpawadee, S.;Saenthaweesuk, S.;Chantiratikul, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.12
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    • pp.1661-1666
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    • 2009
  • The objective of this study was to determine the effect of Se-enriched bean sprout, Se-enriched yeast and sodium selenite on productivity, egg quality and egg Se concentrations in laying hens. Using a Completely Randomized Design, 144 Rohman laying hens at 71 weeks of age were divided into four groups. Each group consisted of four replicates and each replicate contained nine hens. The dietary treatments were T1: control diet, T2: control diet plus 0.3 mg Se/kg from sodium selenite, T3: control diet plus 0.3 mg Se/kg from Se-enriched yeast, T4: control diet plus 0.3 mg Se/kg from Se-enriched bean sprout. The results showed that there was no significant difference (p>0.05) in feed intake, egg production and egg quality among treatments. Selenium supplementation from Seenriched yeast and Se-enriched bean sprout markedly increased (p<0.05) egg Se concentration as compared to the control and sodium selenite groups. The results indicated that Se-enriched bean sprout could be used as an alternative Se source in diets of laying hens.

The Effects of Dietary Biotite V Supplementation as an Alternative Substance to Antibiotics in Growing Pigs

  • Chen, Y.J.;Kwon, O.S.;Min, B.J.;Son, K.S.;Cho, J.H.;Hong, J.W.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.11
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    • pp.1642-1645
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    • 2005
  • This study was conducted to investigate the effects of Biotite V supplementation on growth performance, nutrients digestibility and blood constituents and to evaluate whether Biotite V could replace an antibiotics in growing pigs diet. One hundred twenty pigs with initial body weight of 18.35${\pm}$0.15 kg were used in a 28 days growth trial. Pigs were allotted to four treatments by sex and body weight in a randomized complete block design. There were six replicate pens per treatment and five pigs per pen. Four dietary treatments were: 1) NC (basal diet without antibiotics), 2) PC (basal diet+0.1% CTC), 3) NCBV (NC diet+0.5% 200 mesh Biotite V) and 4) PCBV (PC diet+0.5% 200 mesh Biotite V). Through the entire experimental period, ADG tented to increase in NCBV and PCBV treatments compared to NC and PC treatments respectively, but no significant differences were observed (p>0.05). ADFI was slightly lower in NCBV and PCBV treatments than that in NC and PC treatments without significant differences (p>0.05). Gain/feed in PC and PCBV treatments was improved significantly compared to NC treatment (p<0.05). N and Ca digestibilities were higher in PCBV treatments than those in PC treatment (p<0.05). DM and P digestibilities were not affected by the addition of Biotite V (p>0.05). RBC, HCT, Hb, lymphocyte and monocyte were increased numerically in NCBV and PCBV treatments compared to NC and PC treatments (p>0.05). WBC was lower in treatment groups than that in NC treatment, but no significant differences were observed (p>0.05). In conclusion, dietary supplementation of Biotite V can better the gain/feed and some of the nutrients digestibilities in growing pigs. It has a possibility to replace antibiotics in swine diet.

Generation and Characterization of a Stable Full-Length Ecotropic Murine Leukemia Virus Molecular Clone that Produces Novel Phenotypes to Fv1 Restriction

  • Bae, Eun-Hye;Park, Sung-Han;Park, Sang-Min;Park, Jin-Woo;Lim, Mi-Suk;Jung, Yong-Tae
    • Journal of Microbiology and Biotechnology
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    • v.18 no.4
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    • pp.799-804
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    • 2008
  • Retrovirus tropism can be restricted by host cell factors such as Fv1, TRIM5${\alpha}$, and LvI that inhibit infection by targeting the incoming viral capsid. The Fv1 gene inhibits murine leukemia virus infection in mice, but the precise mechanism of Fv1-mediated restriction is poorly understood. Our previous studies had demonstrated that Fv1-mediated viral tropism can be determined within the capsid protein at position 114. To study the interaction between Fv1 and CA, we introduced amino acid substitution and deletion at this site in the N-tropic AKV capsid gene. The mutated two-LTR proviral DNAs were introduced into SC-1 cells by transfection. After transfection, cell supernatants collected from transfected cells were tested for host range susceptibility. The result indicated that substitution of amino acids did not alter tropism, but the deletion of 114His produced a virus with unusual tropism. The novel phenotype produced here failed to replicate in Fv1-expressing cells. This mutant virus showing such an extreme restriction pattern would be useful for studying the mechanism of Fv1-mediated restriction.

Initial Responses of Quercus serrata Seedlings and Forest Understory to Experimental Gap Treatments

  • Cho, Yong-Chan;Kim, Jun-Soo;Lee, Chang-Seok;Cho, Hyun-Je;Bae, Kwan-Ho
    • Journal of Ecology and Environment
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    • v.32 no.2
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    • pp.87-96
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    • 2009
  • Pinus thunbergii plantations in Pohang-si, Gyeongsangbuk-do, Korea, are of low ecological quality, with arrested succession and a high proportion of ruderal species. To improve the quality of the habitat, we created canopy gaps ($\sim42\;m^2$) and monitored changes in abiotic (light availability, canopy openness) and biotic (survival and growth of seedlings and understory communities) variables in 2007 and 2008 in plots that had received one of five types of treatment: cutting of canopy trees and removal of the understory (CU), cutting of canopy trees only (C), girdling of canopy trees and removal of the understory (GU), girdling of canopy trees (G) or control. Each treatment was applied to three replicate plots. Abiotic variables did not significantly differ among treatments. Survival rates of target species were slightly lower in the CU, G and control conditions. Based on logistic regression analysis, the only significant growth factor affecting survival was height growth. Positive effects of seedling height and leaf area growth on survival were also detected, but did not reach statistical significance. In treatment G, gradual improvement of overstory conditions and mitigation of competition by limitation of disturbance to the understory community were likely to have promoted seedling growth. There were no significant effects of gap treatments on changes in species abundance (cover and richness) and composition of understory between the study years. This result implies that the small gaps created in our study may be below the threshold size to affect understory growth. However, the results of this study are based on a short-term investigation of only two years. Long-term research is strongly recommended to clarify the effects of gap treatment on plant communities in afforested areas.

Effects of Dietary Persimmon Peel and its Ethanol Extract on the Production Performance and Liver Lipids in the Late Stage of Egg Production in Laying Hens

  • Oh, S.T.;Zheng, L.;Shin, Y.K.;An, B.K.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.2
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    • pp.260-265
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    • 2013
  • The purpose of this study was to investigate the dietary effects of persimmon peel (PP) and PP ethanol extract (PPE) on egg production, egg quality, and liver lipids in the late stage of egg production in laying hens. One hundred and twenty 50-wk-old Hy-Line Brown layers (n = 120) were fed different diets. Four replicate groups of 6 hens each were randomly assigned to 5 dietary treatments. The 5 dietary treatments were as follows: i) CON, basal diet; ii) PP 0.15, CON+0.15% PP (0.035% tannin); iii) PP 0.5, CON +0.5% PP (0.117% tannin); iv) PPE 0.075, CON+0.075% PPE (0.03% tannin); and v) PPE 0.25, CON+0.25% PPE (0.11% tannin). The total tannin concentration of PPE was higher (p<0.05) than that of PP. Egg production in the PP 0.5 group was higher than in the other groups. Egg production and mass of hens in the PPE 0.25 group showed a greater decrease than that in the other groups (p<0.05). Eggshell color in the PP 0.15, PP 0.5, and PPE 0.075 groups was lighter than that of the control group (p<0.05). The Haugh unit for the groups that were fed PP and PPE were significantly higher than that in the other groups after 7 d of storage (p<0.05). Therefore, PP seems an effective feed additive for improving the production performance and egg quality in late stage laying hens.

Effect of High Phytase Inclusion Rates on Performance of Broilers Fed Diets Not Severely Limited in Available Phosphorus

  • Dos Santos, Tiago Tedeschi;Srinongkote, S.;Bedford, M.R.;Walk, C.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.2
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    • pp.227-232
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    • 2013
  • Phytate is not only an unavailable source of phosphorus (P) for broilers but it also acts as an anti-nutrient, reducing protein and mineral absorption, increasing endogenous losses and reducing broiler performance. The objective of this study was to investigate the anti-nutritional effects of phytate by including high levels of phytase in diets not severely limited in available P. A total of 768 male Arbor Acres broilers were distributed in six treatments of eight replicate pens of 16 birds each consisting of a positive control diet (PC), positive control with 500 FTU/kg phytase, negative control (NC) diet with lower available P and calcium (Ca) levels and the same NC diet with 500, 1,000 or 1,500 FTU/kg phytase. Body weight gain (BWG), feed intake (FI), feed conversion ratio (FCR) and mortality were determined at 21 and 35 d of age while foot ash was determined in four birds per pen at 21 d of age. FI, FCR and foot ash where not affected by the lower mineral diets at 21 d of age nor by the enzyme inclusion but broilers fed lower Ca and available P diets had lower BWG. At 35 d of age no difference was observed between broilers fed the positive or NC diets but broilers fed 500, 1,000 and 1,500 FTU/kg on top of the NC diet had better FCR than broilers fed the positive control diet. When compared to birds fed a diet adequate in P, birds fed the same diet included with 500, 1,000 and 1,500 FTU/kg of phytase in marginally deficient available P and Ca diets had an improvement of performance. These results support the concept that hydrolysing phytate and reducing the anti-nutritional effects of phytate improves bird performance on marginally deficient diets that were not covering the P requirement of birds.

Replication of Hybrid Micropatterns Using Selective Ultrasonic Imprinting (선택적 초음파 임프린팅을 사용한 복합 미세패턴의 복제기술)

  • Lee, Hyun Joong;Jung, Woosin;Park, Keun
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.1
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    • pp.71-77
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    • 2015
  • Ultrasonic imprinting is a micropattern replication technology for a thermoplastic polymer surface that uses ultrasonic vibration energy; it has the advantages of a short cycle time and low energy consumption. Recently, ultrasonic imprinting has been further developed to extend its functionality: (i) selective ultrasonic imprinting using mask films and (ii) repetitive ultrasonic imprinting for composite pattern development. In this study, selective ultrasonic imprinting was combined with repetitive imprinting in order to replicate versatile micropatterns. For this purpose, a repetitive imprinting technology was further extended to utilize mask films, which enabled versatile micropatterns to be replicated using a single mold with micro-prism patterns. The replicated hybrid micropatterns were optically evaluated through laser light images, which showed that versatile optical diffusion characteristics can be obtained from the hybrid micropatterns.

EFFECTS OF EARLY FEED RESTRICTION ON GROWTH PERFORMANCE AND BODY COMPOSITION IN BROILERS

  • Santoso, U.;Tanaka, K.;Ohtani, S.;Youn, B.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.3
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    • pp.401-410
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    • 1993
  • Day-old male and female broilers obtained from commercial strain (Hubbard) were used in this study. At 7 days of age, all chicks were weighed individually (female 0.11 kg and male 0.12 kg in average) and divided into 5 group of 45 birds each group with no replicate in each treatment group as follows: 1. fed ad libitum; 2. fed 75% ad libitum; 3. fed 65% ad libitum; 4. fed 55% ad libitum; 5. fed 45% ad libitum. Chicks were restricted for 10 days (d 7 through 17) of a 56-day trial. Chicks were fed a commercial starter diet (crude protein (CP) 23.8% and metabolizable energy (ME) 3,070 kcal/kg) for 21 days, and commercial finisher diet (CP 20% and ME 3,160 kcal/kg) from 22 to 56 days of age. When chicks were feed-restricted at an early age, compensatory growth did not immediately occur following refeeding. Body weights of restricted chicks were not equal to the ad libitum chicks before 49 days of age. At 56 dyas of age, body weights of restricted chicks were heavier (p<0.01). Abdominal fat values of female chicks fed ad libitum was not significantly different from those fed 45% to 75% ad libitum. Carcass fat values of restricted female chicks were lower than those of control chicks when female chicks were fed either 55% or 45% ad libitum. Furthermore, feed efficiencies of restricted chicks were better. The response of chicks to early feed restriction might depend on the degree of feed restriction, and sex. Factors contributing carcass fat included energy loss, fat protein ratio of carcass, fatty acid synthesis in the liver and triglyceride content of the liver. The heavier body weights in the restricted chicks might be correlated with the lower growth rate during period of feed restriction.