• Molecules and Cells
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• v.24 no.1
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• pp.1-8
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• 2007

Natural killer (NK) cells play a crucial role in innate immune system and tumor surveillance. NK cells are derived from $CD34^+$hematopoietic stem cells and undergo differentiation via precursor NK cells in bone marrow (BM) through sequential acquisition of functional surface receptors. During differentiation of NK cells, many factors are involved including cytokines, membrane factors and transcription factors as well as microenvironment of BM. NK cells express their own repertoire of receptors including activating and inhibitory receptors that bind to major histocompatibility complex (MHC) class I or class I-related molecules. The balance between activating and inhibitory receptors determines the function of NK cells to kill targets. Binding of NK cell inhibitory receptors to their MHC class I-ligand renders the target cells to be protected from NK cell-mediated cytotoxicity. Thus, NK cells are able to discriminate self from non-self through MHC class I-binding inhibitory receptor. Using intrinsic properties of NK cells, NK cells are emerging to apply as therapeutic agents against many types of cancers. Recently, NK cell alloactivity has also been exploited in killer cell immunoglobulin-like receptor mismatched haploidentical stem cell transplantation to reduce the rate of relapse and graft versus host disease. In this review, we discuss the basic mechanisms of NK cell differentiation, diversity of NK cell receptors, and clinical applications of NK cells for anti-cancer immunotherapy.

• Molecules and Cells
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• v.27 no.2
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• pp.225-235
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• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• Journal of Veterinary Science
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• v.21 no.1
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• pp.18.1-18.17
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• 2020

Cat vocalizes to communicate with another and express their internal states. The vocal repertoire of the cat is wide and up to 21 different vocalizations have been described in the literatures. But it is more than probable that the repertoire contains more types of vocalizations. An ethogram was created in this paper describing the actual known vocalisations of the domestic cat based on an auditory classification. However, the audiogram allows also a visual classification which can increase the accuracy of vocalization differentiation. The classification can be risky as it is sometimes unclear if different types of vocalizations are produced in different environments or if a unique type of vocalization is used with variation in the acoustic parameters. As an example, isolation calls produced by kittens differ depending on the context. The environment has an important impact on the vocal behaviour and thus feral cats and pet cats vocalize differently. Pet cats are thus able to create an efficient communication with humans thanks to the flexibility of vocalisation behaviours. This review allowed us to create a simple model of the cat vocal repertory.

• Proceedings of the Korea Contents Association Conference
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• 2018.05a
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• pp.97-98
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• 2018

재즈 연주자들이 음반에 많이 녹음한 재즈 스탠더드 곡을 대상으로 레퍼토리의 장르를 분류하여 선정한 곡을 중심으로 다른 버전의 편곡 특성을 연구한다. 레퍼토리 곡은 재즈 스탠더드 원곡에서 블루스, 스윙, 발라드, 비밥, 라틴의 5가지 장르로 분류되며, 대부분 발라드와 스윙이다. 레퍼토리의 곡 분류를 통해 같은 곡이 다른 버전에서 어떻게 다르게 연주되었는지 보기위해 여성 재즈보컬들에 의해 녹음된 음반을 중심으로 각 버전의 특징을 분석한다. 이 연구를 수행하여 각 여성 재즈 보컬들의 녹음된 곡에서 다른 버전의 편곡 특성을 도출한다.

• The Journal of the Korea Contents Association
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• v.14 no.12
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• pp.694-705
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• 2014

Recently media use is in the period of upheaval because of the advent of smart media. At this present, there are many discussions about the multiplexing or convergence of media use and these effects of everyday life, based on the theory of 'media repertoires'. But many researches just divide media repertoire categories and show media use hours by these categories. To have more mighty power of explanation, the study about media repertoires must execute both a static analysis of media use hours and a dynamic analysis of media use by media repertoire categories in everyday life.

• Korean journal of communication and information
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• v.58
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• pp.154-177
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• 2012

Considering the growing importance of TV favorite channel setting function due to the rapid increase of number of TV channels, this study tried to find factors which affect the attitude toward and intention to use the favorite channel setting function. As related theories, this study used theory of diffusion of innovation, channel repertoire, channel changing behaviors, and social influences and suggested research hypotheses and questions based on the theories. After collecting data through online survey of 192 general audience, this study empirically analyzed the data by using multiple regression analysis. The result showed that innate innovativeness, relative advantage and social critical mass affected attitude toward using the favorite channel setting function and that compatibility, visibility, social critical mass influenced intention to use the function.

• Molecules and Cells
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• v.39 no.8
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• pp.603-610
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• 2016

Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.12
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• pp.4833-4837
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• 2015

Biomarkers for preclinical diagnosis of cancer are valuable tools for detection of malignant tumors at early stages in groups at risk and screening healthy people, as well as monitoring disease recurrence after treatment of cancer. However the complexity of the body's response to the pathological processes makes it virtually impossible to evaluate this response to the development of the disease using a single biomarker that is present in the serum at low concentrations. An alternative approach to standard biomarker analysis is called immunosignature. Instead of going after biomarkers themselves this approach rely on the analysis of the humoral immune response to molecular changes associated with the development of pathological processes. It is known that antibodies are produced in response to proteins expressed during cancer development. Accordingly, the changes in antibody repertoire associated with tumor growth can serve as biomarkers of cancer. Immunosignature is a highly sensitive method for antibody repertoire analysis utilizing high density peptide microarrays. In the present review we discuss modern methods for antibody detection, as well as describe the principles and applications of immunosignature in research and clinical practice.

• The Korean Journal of Zoology
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• v.39 no.1
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• pp.89-97
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• 1996

Songs of the Yellow-throated Bunting (Emberiza elegans) and the Gray-headed Bunting (Emberiza fucata) in allopatric populations in Gangnae-meon, Cheongwon-gun, Chungbuk in Korea, were recorded during the breeding season and analyzed in sound spectroraphs. Males of E. elegans and E. fucata were tested to investigate whether territorial males can discriminate between neighbor and stranger based on playback of natural and artificial song repertoires. In addition, E. fucata was stimulated by playback of only the individually specific section as well as of only the posterior portion of the song. Males of E. elegans were able to discriminate individually between neighbor and stranger in response to natural song repertoires, but they did not respond to playback of the artificial song repertoires of neighbor and stranger. Males of E. fucata were able to discriminate individually between neighbor and stranger in response to natural, artificial song repertoires, and the anterior section of the song, while males did not respond to playback of the posterior section of the song.

• Journal of Korean Library and Information Science Society
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• v.54 no.3
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• pp.249-270
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• 2023

The purpose of this study is to identify the characteristics of school library services using the RGT (repertory grid technique), and to examine whether there are differences in the perceptions and internal experiences of school library services between human resources (librarian teachers, subject teachers, and librarians). I used indicators such as element intensity, construct extremity, discrepant constructs, and implicative dilemmas to analyze the characteristics of school library services. The elements of the repertory grid were set to 9, and 14 constructs were set through focus group interviews with 3 librarian-teachers. The GRIDCOR 6.0, which can be accessed online, was used to complete the repertory grid for 30 graduate students of the College of Education, and 6 of them were selected and analyzed, considering the demographic characteristics. From the perspective of element intensity, it was found that the following school library services are important and influential: resource management, teacher-librarian collaboration, and reading and information counseling services. The clarity of one's role, the actual performance, and self-regulatory ability were ranked high from the perspective of constructs intensity.