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Pycnidiospore Production and Dispersal from the Warts Produced by Infection of Botryosphaeria dothidea on Apple Stems

  • Park, Chang-Hee;Yang, Hee-Jung;Hyun Woo;Kim, Dai-Gee;Uhm, Jae-Youl
    • The Plant Pathology Journal
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    • v.15 no.6
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    • pp.330-334
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    • 1999
  • Applying the method of quantitative analysis of pycnidiospore from the detached warts produced by the infection of Botryosphaeria dothidea on apple stems, repeated productivity of spores within the detached warts, variations in the amount of spores within the detached warts, variations in the amount of spores by the length of induction time for sporulation, and the effects of temperature and moisture on the sporulation were investigated. In addition to these experiment, the changes in the state of spores within the pycnidia contained in the warts accompanied by the induction of sporulation and dispersal of spores were also investigated. When detached warts were kept in moist conditions, the sporulation and discharge of spores were also investigated. When detached warts were kept in moist conditions, the sporulation and discharge of spores could be repeated several times, and the amount of spores were almost constant after each repeat of sporulation induction and dispersal of spores in a given period. The fact that the pycnidia filled with spores were observed at considerable rates within the warts which were subjected to the shaking in the water to release spores indicated that the spores might never be released until the pycnidia were fully matured. From the high rate of empty pycnidia even in the warts which were kept in moist conditions for induction of sporulation, the pycnidiospores might be produced through the development of new pycnidia. A considerable amount of pycnidiospores were produced at $5^{\circ}$, and the sporulation was accelerated with the rise of temperature until $35^{\circ}$. When the warts were supplied with sufficient moisture, sporulation was further accelerated. The results obtained in these experiment will be applied in developing the method for assessing the inhibitory efficacies of fungicides on the sporulation of this fungus, with which a new control measure would be developed.

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Evaluation of Optimum Genetic Contribution Theory to Control Inbreeding While Maximizing Genetic Response

  • Oh, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.3
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    • pp.299-303
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    • 2012
  • Inbreeding is the mating of relatives that produce progeny having more homozygous alleles than non-inbred animals. Inbreeding increases numbers of recessive alleles, which is often associated with decreased performance known as inbreeding depression. The magnitude of inbreeding depression depends on the level of inbreeding in the animal. Level of inbreeding is expressed by the inbreeding coefficient. One breeding goal in livestock is uniform productivity while maintaining acceptable inbreeding levels, especially keeping inbreeding less than 20%. However, in closed herds without the introduction of new genetic sources high levels of inbreeding over time are unavoidable. One method that increases selection response and minimizes inbreeding is selection of individuals by weighting estimated breeding values with average relationships among individuals. Optimum genetic contribution theory (OGC) uses relationships among individuals as weighting factors. The algorithm is as follows: i) Identify the individual having the best EBV; ii) Calculate average relationships ($\bar{r_j}$) between selected and candidates; iii) Select the individual having the best EBV adjusted for average relationships using the weighting factor k, $EBV^*=EBV_j(1-k\bar{{r}_j})$ Repeat process until the number of individuals selected equals number required. The objective of this study was to compare simulated results based on OGC selection under different conditions over 30 generations. Individuals (n = 110) were generated for the base population with pseudo random numbers of N~ (0, 3), ten were assumed male, and the remainder female. Each male was mated to ten females, and every female was assumed to have 5 progeny resulting in 500 individuals in the following generation. Results showed the OGC algorithm effectively controlled inbreeding and maintained consistent increases in selection response. Difference in breeding values between selection with OGC algorithm and by EBV only was 8%, however, rate of inbreeding was controlled by 47% after 20 generation. These results indicate that the OGC algorithm can be used effectively in long-term selection programs.

Percutaneous Nephrostomy Using 18 Guage Puncture Needle (18게이지 천자침을 이용한 경피신루설치술)

  • Kim, Jae-Woon;Kim, Jin-Woo;Cho, Jae-Ho;Chang, Jae-Chun;Park, Bok-Hwan
    • Journal of Yeungnam Medical Science
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    • v.15 no.1
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    • pp.159-163
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    • 1998
  • Percutaneous nephrostomy was performed using 18 guage pucture needle under ultrasonic guidance in 58 patients, 98 cases with hydronephrosis (bilateral; 15 cases, repeat nephrostomy; 25 cases). The causes of the hydronephrosis were malignancy(33 patients, 65 cases), benign diseases(18 patients, 25 cases), and unknown causes(7 patients, 8 cases). Successful nephrostomy was achieved in a11 cases. Major complication was not found, but seven(7.1%) minor complications, such as gross hematuria(four cases), perirenal urine leakage(two cases), and fever(one cases) were developed. The complication rate in our study was similar to that of other studies using 21 guage puncture needle. In conclusion, we think that the percutaneous nephrostomy using 18 guage puncture needle is a simple, safe, and cost and time effective procedure and it can replace the method using 21 guage puncture needle.

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Influence of Festival Visitor's Satisfaction on Revisit and Word of Mouth Intention (축제만족도가 재방문의사와 추천의사에 미치는 영향)

  • Min, Dong-Kyoo
    • The Journal of the Korea Contents Association
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    • v.8 no.11
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    • pp.356-363
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    • 2008
  • The purpose of this study is to examine the difference between festival visitors motivation and satisfaction by visitors' past experiences at Gim-po Culture & Art Festival 2008, Which is the 25th, is holding soon. Gim-po Culture & Art Festival has unique aspect that it is organised various experience programs and items which stress the importance of environment mix in Han river, Gim-po's port, totemism, agriculture and so on. A Study is analysed through research that is consisted of motivation and satisfaction factor's item of 23 local festival visitor to study factors of determination of local festival visitor's motivation and satisfaction level. Data were collected with a structured questionnaire at the 24th Gim-po Culture & Art Festival, 695 sample were used for statistical analysis. This research data is analysed by 4 statistical techniques, which are t-test, Cross-tab analysis, reliability analysis, factor analysis and regression analysis. As the results of this research, festival visitor's satisfaction were composed of four factors; festival theme, physical facilities, festival information service, souvenir and food. Festival satisfaction and motivation appeared the difference between first-time visitors and repeat visitors. The result of this paper may help festival planner and marketer for festival marketing strategy.

Differential Gene Expression of Soybean[Glycine max(L.) Merr.] in Response to Xanthomonas axonopodis pv. glycines by Using Oligonulceotide Macroarray

  • Van, Kyujung;Lestari, Puji;Park, Yong-Jin;Gwag, Jae-Gyun;Kim, Moon-Young;Kim, Dong-Hyun;Heu, Sung-Gi;Lee, Suk-Ha
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.147-158
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    • 2007
  • Xanthomonas axonopodis pv. glycines(Xag) is a pathogen that causes bacterial leaf pustule(BLP) disease in soybeans grown in Korea and the southern United States. Typical and early symptoms of the disease are small, yellow to brown lesions with raised pustules that develop into large necrotic lesions leading to a substantial loss in yield due to premature defoliation. After Xag infects PI 96188, only pustules without chlorotic haloes were observed, indicating the different response to Xag. To identify differentially expressed genes prior to and 24 hr after Xag inoculation to PI 96188 and BLP-resistant SS2-2, an oligonucleotide macroarray was constructed with 100 genes related to disease resistance and metabolism from soybean and Arabidopsis. After cDNAs from each genotype were applied on the oligonucleotide macroarrays with three replicates and dye swapping, 36 and 81 genes were expressed as significantly different between 0 hr and 24 hr in PI 96188 and SS2-2, respectively. Six UniGenes, such as the leucine-rich repeat protein precursor or 14-3-3-like protein, were selected because they down-regulated in PI 96188 and up-regulated in SS2-2 after Xag infection, simultaneously. Using tubulin and cDNA of Jangyeobkong(BLP-susceptible) as controls, the oligonucleotide macroarray data concurred with quantitative real-time RT-PCR(QRT RT-PCR) results in most cases, supporting the accuracy of the oligonucleotide macroarray experiments. Also, QRT RT-PCR data suggested six candidate genes that might be involved in a necrotic response to Xag in PI 96188.

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The Optimal respiration training based work-related stress relief system (최적 호흡 훈련기반 업무 스트레스 완화 시스템)

  • Lee, Yangwoo;Whang, MinCheol
    • Science of Emotion and Sensibility
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    • v.17 no.2
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    • pp.85-90
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    • 2014
  • The purpose of the study is to develop self-management system that people can enhance physical and psychological health through repeating by themselves to relieve work-related stress. The regular respiration can help homeostasis of heart to maintain. Also the effect can be stabilized from irregular heart rhythm by work-related stress. People have optimal respiration cycle to stabilize heart rhythm and repeat training using their RSP(respiration) time including expiration and inhalation. This system is not only offering optimal respiration training service but also finding optimal respiration cycle. The adults who have stress from work participated in verification experiment. This study expects to help those people who are workers related to call center jobs in emotional labor can relieve their stress. It can also help to enhance their own health and increase their work efficiency.

Analysis of the operation effciency with the application of fabirc design CAD system (직물설계 CAD System활용에 따른 작업성 분석)

  • Kim, Hee-Sam;Kim, Mi Sun;Lee, Young Hee
    • Journal of the Korean Society of Industry Convergence
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    • v.8 no.1
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    • pp.11-17
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    • 2005
  • This study was performed to analysis the operation process when fabric pattern design was done by the use of CAD system compared with the manual work in order to determine the operation efficiency with the application of fabric design CAD system. The results of the study were as follows: 1. since 160,000 colors were supported by CAD system, color proposed by consumer was able to match exactly according to the its design. However, exact color matching was not possible by manual work. 2. Woven state of back of pattern design could be identified simultaneously with face of it for CAD system, while face and back of the fabrics should be designed separatedly in case of manual work. 3. Since the combination of warp and filling yarn was compatible with the fabric density in one repeat unit in CAD system, exact size of pattern design to be woven was able to expressed. 4. Only simple graphical expression by manual work was seen, while with the CAD system, texture and shade effect as well as graphical expression could be expressed and so fault could be reduced in advance with the simulation of actual feeling of fabrics in the screen. In conclusion, when CAD system will be introduced to the textile industry, operation time of designing pattern can be reduced. Since the operation is easy and simple, a beginner can operate CAD system easily. Thus, production and wage costs can be saved and this can be related directly with the improvement of productivity which is the main purpose of introducing CAD system.

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Contouring of Forehead and Temple Area with Auto-Fat Injection (지방주입술을 이용한 전두 및 측두 부위의 윤곽교정술)

  • Kang, Jae-Hoon;Jung, Seung-Won;Lee, Yong-Hae;Kook, Kwang-Sik
    • Archives of Plastic Surgery
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    • v.38 no.2
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    • pp.166-172
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    • 2011
  • Purpose: Facial contouring surgery for improving congenital, acquired deformity and senile change were attempt in past. Recently contouring surgery became more interested subject for improving the flat forehead and temple area. Many synthetic materials were used such as Collagen, silicon, polyacrylamide gel as liquid form and Gore-tex, silicon implant, endotine as solid form. But, these synthetic implants associate complications as foreign body reaction, infection, displacement, granuloma formation and absorption. Auto-fat injection are used for disfigurement of many part of body. We did auto-fat injection for facial contouring of forehead and temple region. Auto-fat injection is suitable without foreign body reaction, displacement, and toxic reaction. Also auto-fat is relatively simple to obtain from patient and less expensive and able to repeat surgeries. Methods: From 2006 to 2009, 150 patients were treated with Auto-fat injection for facial contouring. For follow up, we sent questionnaire to all patients but 110 patients returned answer sheets. The patients consisted of 20 male patients and 90 female patients with an age ranged from 26 to 60, and the mean 43. Fat tissue were injected 6-8 cc in forehead, 7-12 cc in temple area and fat were harvested from thigh and abdomen. Results: In follow up, all patients, showed absorption of injected fat varied degree and except two patients all patients underwent secondary fat injection. Complications were minimal and neuropraxia of facial nerve were recovered. Most of the patients were satisfied with result of procedure, and answered that they recommend same procedure to their friends and will do surgery again. Conclusion: Auto-fat injections were implemented for facial contouring in 150 patients and obtained satisfactory result. Auto-fat injection is relatively easy procedure and applicable widely. Even though, by passing time, some of the injected fats are absorbed, auto-fat injection could be choice of treatment for contouring forehead and temple. With accumulations of cases and development of surgical technique, better result could be expected.

Experience with Conventional Radiofrequency Thermorhizotomy in Patients with Failed Medical Management for Trigeminal Neuralgia

  • Singh, Sarita;Verma, Reetu;Kumar, Manoj;Rastogi, Virendra;Bogra, Jaishree
    • The Korean Journal of Pain
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    • v.27 no.3
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    • pp.260-265
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    • 2014
  • Background: To evaluate the results of conventional radiofrequency thermorhizotomy (CRT) for trigeminal neuralgia (TN) in patients with failed medical management. Methods: Patients with Trigeminal neuralgia who were referred to us for 'limited intervention' during the time frame July-2011 to Jan-2013 were enrolled for this study. CRT was administered by the Sweet technique. Pain relief was evaluated by the principle investigator. Results: Eighteen patients were enrolled and completed a mean follow-up of 18.0 months. Pain relief was observed in 14 of 18 (77.8%) patients on the post-operative day, 14 of 18 (77.8%) at 1-month follow-up, 14 of 17 (82.4%) at 3-months follow-up, 12 of 15 (80%) at 6-months follow-up, 7 of 11 (63.6%) at 1-year follow-up and 2 of 6 (33.3%) 1.5 years of follow-up. Four patients required a repeat cycle of CRT; two at six months of follow-up and two at one year of follow-up. One patient was transferred for surgical intervention at six months of follow-up. Side-effects included facial hypoesthesia (n = 6); nausea/vomiting (n = 2), diminished corneal reflex (n = 13) and difficulty in chewing (n = 11). Severity of adverse effects gradually diminished and none of the patients who are beyond 6 months of follow-up have any functional limitation. Conclusions: CRT is an effective method of pain relief for patients with Trigeminal neuralgia. Successful outcome (excellent or good) can be expected in 66.7% of patients after first cycle of CRF. The incidence and severity of adverse effects is less and the procedure is better tolerated by the patients.

Choosing Optimal STR Markers for Quality Assurance of Distributed Biomaterials in Biobanking

  • Chung, Tae-Hoon;Lee, Hee-Jung;Lee, Mi-Hee;Jeon, Jae-Pil;Kim, Ki-Sang;Han, Bok-Ghee
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.32-37
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    • 2009
  • The quality assurance (QA) is of utmost importance in biobanks when archived biomaterials are distributed to biomedical researchers. For sample authentication and cross-contamination detection, the two fundamental elements of QA, STR genotyping is usually utilized. However, the incorporated number of STR markers is highly redundant for biobanking purposes, resulting in time and cost inefficiency. An index to measure the cross-contamination detection capability of an STR marker, the mixture probability (MP), was developed. MP as well as other forensic parameters for STR markers was validated using STR genotyping data on 2328 normal Koreans with the commercial AmpFlSTR kit. For Koreans, 7 STR marker (D2S1338, FGA, D18S51, D8S1179, D13S317, D21S11, vWA) set was sufficient to provide discrimination power of ${\sim}10^{-10}$ and cross-contamination detection probability of ${sim}1$. Interestingly, similar marker sets were obtained from African Americans, Caucasian Americans, and Hispanic Americans under the same level of discrimination power. Only a small subset of commonly used STR markers is sufficient for QA purposes in biobanks. A procedure for selecting optimal STR markers is outlined using STR genotyping results from normal Korean population.