• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.870-875
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• 2015

The purpose of this study was to investigate the alternative splicing in equine cordon-bleu WH2 repeat protein-like 1 (COBLL1) gene that was identified in horse muscle and blood leukocytes, and to predict functional consequences of alternative splicing by bioinformatics analysis. In a previous study, RNA-seq analysis predicted the presence of alternative spliced isoforms of equine COBLL1, namely COBLL1a as a long form and COBLL1b as a short form. In this study, we validated two isoforms of COBLL1 transcripts in horse tissues by the real-time polymerase chain reaction, and cloned them for Sanger sequencing. The sequencing results showed that the alternative splicing occurs at exon 9. Prediction of protein structure of these isoforms revealed three putative phosphorylation sites at the amino acid sequences encoded in exon 9, which is deleted in COBLL1b. In expression analysis, it was found that COBLL1b was expressed ubiquitously and equivalently in all the analyzed tissues, whereas COBLL1a showed strong expression in kidney, spinal cord and lung, moderate expression in heart and skeletal muscle, and low expression in thyroid and colon. In muscle, both COBLL1a and COBLL1b expression decreased after exercise. It is assumed that the regulation of COBLL1 expression may be important for regulating glucose level or switching of energy source, possibly through an insulin signaling pathway, in muscle after exercise. Further study is warranted to reveal the functional importance of COBLL1 on athletic performance in race horses.

• Journal of Radiation Protection and Research
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• 제31권3호
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• pp.155-164
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• 2006

액체섬광계수기를 이용하여 환경시료를 분석하는 모든 과정에서 계측 조건 및 방법론 차이에 의한 불확실성이 존재할 수 있을 것으로 판단하여 본 연구에서는 이에 대한 평가를 정량적으로 수행함으로써 비교적 정확하게 환경시료를 분석하고자 하였다. 이를 위해 결과에 영향을 미칠 수 있는 변수를 도출한 후 각 변수에 대한 영향을 평가한 결과, 분석시료를 제조하는 과정에서의 인적 및 물리적인 불확실성은 무시할 정도로 미미하였고, 측정용기의 무게 차이에 따른 영향은 나타나지 않았다. 외부선원의 조사시간을 단계적으로 증가시킨 결과 시간이 증가함에 따라 데이터의 분산 정도는 감소하면서 $75{\sim}90$ sec에서 포화상태에 도달하였고, Repeat 방법이 Replicate 방법에 비해 데이터의 신뢰성이 높게 나타났다. 또한 방치시간에 따른 영향을 평가한 결과 분석 냉 암소에서 약 1,000 min 이상 방치시킨 후 시료에 대한 분석을 수행해야만 잔상 및 이상 유동에 의한 영향은 거의 없는 것을 알 수 있었고, 결과에 대한 검증을 수행하기 위해 방사능 오차분석과 함께 Chi-square test를 수행한 결과 신뢰성 있는 결과를 보여주었으며 이러한 분석 및 검증 결과에 근거하여 계측 결과에 대한 오차 및 불확실성을 감소시킬 수 있었다.

• 한국산림과학회지
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• 제90권6호
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• pp.747-755
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• 2001

본 연구는 산촌지역축제인 아카시아벌꿀축제에 대하여 방문객의 관광지 이미지의 구성요인을 도출하고, 방문객의 특성에 따라 관광지 이미지의 유의미한 차이가 있는 지를 분석하였다. 설문조사는 축제기간인 2001년 5월 12일에서 15일까지 축제장을 방문한 394명의 관광객을 대상으로 실시하였으며, 관광지 이미지 평가를 위한 설문항목은 선행연구를 기초로 20개를 설정하고 5점 Likert 척도를 적용하여 요인분석을 실시하였다. 아카시아벌꿀 축제의 관광지 이미지는 크게 환경, 태도 시설, 축제 프로그램, 그리고 상품요인 둥 4개 요인으로 구성되었다. 방문객의 특성에 따른 관광지 이미지의 변화는 최초방문객과 재방문객, 지역주민과 외지인, 그리고 벌꿀상품을 구매한 관광객과 구매하지 않은 관광객 사이에 이미지 평가에 대한 차이가 통계적으로 존재하는 지를 검정하였다. 그 결과 재방문객과 지역주민의 관광지 이미지가 환대성, 축제 내용 및 순서정보 제공과 같은 태도요인에서 긍정적인 것으로 나타났다. 또한 지역 벌꿀을 구매한 집단의 이미지는 구매하지 않은 집단보다도 상품의 가격과 품질뿐만 아니라 환경요인인 지역문화체험, 자연경관, 향수성, 분위기 등에서 긍정적인 것으로 평가되었다. 따라서 축제이미지 향상을 위하여는 주최측인 지방정부는 외부 관광객에게 축제에 대한 효과적인 정보제공과 함께 지역의 자연환경과 연계된 향토성과 체험성을 강조하는 프로그램의 개발이 필요한 것으로 판단된다.

• Animal Bioscience
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• 제34권8호
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• pp.1290-1302
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• 2021

Objective: Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become a hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear. Methods: In this study, the whole transcriptome sequencing of SY follicles with different expression levels of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by quantitative real-time polymerase chain reaction. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles. Results: Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosondowah ankyrin repeat domain family member A (SOWAHA) was the common target gene of three miRNAs and one lncRNA. SOWAHA was mainly expressed in small white (SW) and SY follicles and was affected by follicle stimulation hormone (FSH) treatment in the granulosa cells. Knockdown of SOWAHA inhibited the expression of Wnt family member 4 (Wnt4) and steroidogenic acute regulatory protein (StAR) in the granulosa cells of prehierarchal follicles, while stimulated Wnt4 in hierarchal follicles. Overexpression of SOWAHA increased the expression of Wnt4 in the granulosa cells of prehierarchal follicles, decreased that of StAR and cytochrome P450 family 11 subfamily A member 1 in the granulosa cells of hierarchal follicles and inhibited the proliferation of granulosa cells. Conclusion: Integrated analysis of chicken SY follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection.

• 한국노년학
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• 제29권2호
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• pp.657-667
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• 2009

본 연구는 규칙적인 유산소 운동이 성장에 따른 세포 복제현상에 미치는 영향을 살펴보기 위하여 염색체 말단에 위치한 텔로미어의 길이와 결합단백질인 TRF 2(telomere repeat binding factor 2)의 발현량을 분석하였다. 흰쥐를 대상으로 장기간 운동집단, 단기간 운동집단, 비교집단 그리고 기준수준 검증을 위한 기준집단의 4집단으로 각각 7마리씩 총28마리를 배정하였으며, 수영 운동은 무부하 자유유영의 형태로 주 5회, 매회 1시간씩 실시하였다. 마지막 운동 종료 후 24시간 뒤 쥐를 희생시켜 심장, 간, 가자미근을 적출하여 genomic DNA를 추출, 텔로미어의 길이와 관련단백질인 TRF 2의 발현량을 분석하였다. 연구 결과, 간조직의 텔로미어 길이와 TRF 2 단백질의 발현량은 집단 간 차이를 발견할 수 없었으나, 심장조직의 텔로미어 길이는 장기간 운동집단이 비교집단 및 단기간 운동집단에 비해 길게 나타났고, TRF 2의 단백질 발현 또한 높게 나타나, 장기간의 수영 운동이 성장에 따른 심장의 텔로미어 단축현상을 지연시키는 효과가 있는 것으로 나타났다. 또한, 가자미근에서는 4주령의 기준집단에 비해 비교집단과 단기간 운동집단의 텔로미어의 길이는 유의하게 짧았으나, 장기간 운동집단과는 유의한 차이가 나타나지 않은 것으로 미루어 장기간의 수영 운동이 골격근의 복제적 노화현상도 억제할 수 있는 가능성을 관찰할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1301-1306
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• 2012

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 non-respiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ${\leq}35$ and the ratio of real-time RT-PCR and real-time PCR load was ${\geq}1.51$. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including non-respiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

• 디지털콘텐츠학회 논문지
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• 제19권6호
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• pp.1033-1041
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• 2018

본 연구는 모바일 게임에서 두드러지게 나타나고 있는 게임 패턴에 대한 내용을 담고 있다. 본 연구에서 사용하고 있는 '패턴'이라는 용어는 곽이삭(2016)이 <게임의 사용자 경험에 대한 연구: 게임 공간의 패턴과 속성을 중심으로>에서 사용한 개념을 계승한다. 앞선 연구와의 차이점은 '패턴'은 게이머의 경험 패턴을 '게임 공간'에서 '모바일 게임 시장'으로 범위를 확대하여 사용한다는 점이다. 모바일 게임 시장은 급속도로 성장하고 있으나, 모바일 게임을 개발하는 측면에 있어서는 개발 시간의 축소, 장르 파괴 등으로 인해 게임이 획일화되어가는 경향을 보인다. 때문에 연구자는 이러한 상황을 인지하여 현재의 모바일 게임 시장에서 나타나는 경험의 패턴을 '익숙한 플레이의 반복', '가치 있는 게임 행위', '시간의 가치와 소비', '취향과 선택'의 패턴으로 요약하여 설명하고자 하였다.

• Journal of Korean Neurosurgical Society
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• 제54권3호
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• pp.183-188
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• 2013

Objective : The aim of this study is to assess outcomes during first one year for patients with severe motor weakness caused by lumbar disc herniation that underwent surgical or nonsurgical treatment. Methods : The 46 patients with motor weakness because of lumbar disc herniation who were treated at neurosurgical department and rehabilitation in our hospital from 2006 to 2010, retrospectively. Each group had 26 surgical treatments and 20 conservative treatments. We followed up 1, 3, 6 months and 12 month and monitored a Visual Analogue rating Scale (VAS) of back and leg pain, Oswestry Disability Index (ODI) and degree of motor weakness. We analyzed the differences between surgical and nonsurgical groups using Mann-Whitney U test and repeat measure ANOVA in each follow-up periods. Results : In the recovery of motor weakness, surgical treatment uncovered a rapid functional recovery in the early periods (p=0.003) and no difference between groups at the end of follow-up period was found (p>0.05). In VAS of back and leg, the interaction between time and group was not found (p>0.05) and there was no difference between groups (p>0.05). In ODI, the interaction between time and group was not found (p>0.05) and there was no difference between groups (p>0.05). Conclusion : Surgical treatment for motor weakness caused by herniated intervertebral disc resulted in a rapid recovery in the short-term period, especially 1 month. We think early and proper surgical treatment in a case of motor weakness from disc herniation could be a good way for providing a chance for rapid alleviation.

• Journal of Chest Surgery
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• 제20권4호
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• pp.643-654
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• 1987

The effect of prostacyclin[PGI, ] on myocardial preservation during global ischemia was studied in the isolating working rabbit heart model. Forty hearts underwent a 15 minute period of retrograde nonworking perfusion with Krebs-Henseleit buffer solution [37*C] and were switched over to the working mode for 15 minutes. After baseline measurement of heart rate, peak aortic pressure, aortic flow, and coronary flow, all hearts were subjected to 60 minutes of ischemic arrest at 10*C induced with St. Thomas Hospital cardioplegic solution: Group I had single dose cardioplegia, Croup II double dose, Croup III oxygenated double dose, and Group IV single dose with PCI, infusion [10ng/min./gm heart weight]. Hearts were then revived with 15 minute period of nonworking reperfusion at normothermia, followed by 30 minutes of working perfusion. Repeat measurements of cardiac function were obtained and expressed as a percent of the preischemic baseline values. Oxygen content of arterial perfusate and coronary effluent was measured by designed time interval. Leakage of creatine kinase was determined during post-ischemic reperfusion period. Finally wet hearts were weighed and placed in 120*C oven for 36 hours for measurement of dry weight. In the PGI, treated group [IV], heart rate increased consistently throughout the period of reperfusion from 100*5.0% [p<0.001] to 107*6.2% [p<0.001]. The percent recovery of aortic flow showed 95*5.7% [p<0.001] at the first 3 minute and full recovery through the subsequent time. Coronary flow was augmented significantly in the 3 minute [96*6.2%, p<0.001] and then sustained above baseline values. Among the Croup I, II, and III, all hemodynamic values were significantly below preischemic levels. PGI2 relatively increased oxygen delivery [1.22*0.19ml/min, p<0.001] and myocardial oxygen consumption [0.90*0.13ml/min, p<0.001] during reperfusion period. Leakage of creatine kinase in the PGI2 group was 9.3*1.58IU/15min [p<0.001]. This was significantly lower than Group I [33.0*2.68 IU/15min]. The water content of PCI2 treated hearts [81*0.9%, p<0.001] was also lower than the other groups.

• Journal of Multimedia Information System
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• 제5권3호
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• pp.163-170
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• 2018

We developed a multicast communication packet radio protocol using a time-sharing tablet system ("wireless token ring") to achieve the efficient exchange of files among packet radio terminals attached to swans. This paper provides an overview of the system and the protocol of the packet communications. The packet device forming the main part of the transceiver developed is the Texas Instruments CC2500. This device consists of one call-up channel and one data transmission channel and could improve error frame correction using FEC (forward error correction) with 34.8 kbps MSK and receiving power of at least -64 dBm (output 1 dBm at distance of 200 m using 3 dBi antenna). A time-sharing framework was determined for the wireless token ring using call sign ordinals to prevent transmission right loss. Tests using eight stations showed that resend requests with the ARQ (automatic repeat request) system are more frequent for a receiving power supply of -62 dBm or less. A wireless token ring system with fixed transmission times is more effective. This communication protocol is useful in cases in which frequency resources are limited; the energy consumed is not dependent on the transmission environment (preset transmission times); multiple terminals are concentrated in a small area; and information (position data and vital data) is shared among terminals under circumstances in which direct communication between a terminal and the center is not possible. The method allows epidemiological predictions of avian influenza infection routes based on vital data and relationships among individual birds based on the network topology recorded by individual terminals. This communication protocol is also expected to have applications in the formation of multiple in vivo micromachines or terminals that are inserted into living organisms.