• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.7
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• pp.1536-1541
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• 2009

In this paper, we present a novel pulse train identification method for two step stagger pulse train. Generally radar uses a fixed pulse train, and it is easy for electronic warfare system to measure the pulse repeat interval(PRI) and identify the radar. But it is very difficult to measure the PRI of stagger pulse radar because the pulse interval is periodically changed. We suggest a novel method to measure the PRI and identify the radars using the second deviation of pulse train. This method is faster comparing with Histogram method. We have a good PRI measurement results for 2 step stagger signals.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.8
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• pp.1832-1838
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• 2009

In this paper, we present new signal de-interleaving method for the frequency agility radar in which the carrier frequency is changed irregularly. Generally radar use a fixed carrier frequency, and it is easy for electronic warfare system to de-interleave the radar signal with respect to the frequency, pulse width(PW), and direction of signal arriving(DOA). In frequency agility radar, it is difficult to de-interleave the radar signals according to the carrier frequency because the frequency is changed irregularly. We suggest a good de-interleaving method to identify the frequency agility radar signals in comparison with PRI's of radars. First we calculate pulse repeat Interval(PRI) of radar in linked-list and queue structure and de-interleave the radar signals with PRI, PW, and DOA, then identify the frequency agility radar. When we use the proposed algorism to the frequency agility radar, we have a good de-interleaving results with electronic warfare systems.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2006.06b
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• pp.191-197
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• 2006

Recently, the earth scale disaster is occurring frequently. Under the effects of global warming, the weather has become unseasonable worldwide. Hence, the earth is experiencing unstable condition with many disasters such as storms and flood damages as well as earthquake. Therefore, it is necessary to consider what we can do to prevent disasters. A disaster like earthquake will inevitably occur in view of the probabilities. The active period of earthquakes and the inactive periods repeat alternatively. Consequently, recent warnings indicate that there is a potential risk of massive earthquakes. Consideration of the effects of tsunami to the moored ship is very important. Operational problems such as moored ship motions sometimes become remarkable with large amplitude and long periods in harbor. Moored ship motions may cause the breakage of mooring systems such as mooring lines, fenders or quay. Large and long period moored ship motions are caused by resonant effects. In this paper, the moored ship motions within a harbor by the large-scale tsunami and the effects on the motions and mooring loads with resonant effects are investigated by numerical simulations.

• Molecules and Cells
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• v.44 no.9
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• pp.627-636
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• 2021

The three-dimensional organization of chromatin and its time-dependent changes greatly affect virtually every cellular function, especially DNA replication, genome maintenance, transcription regulation, and cell differentiation. Sequencing-based techniques such as ChIP-seq, ATAC-seq, and Hi-C provide abundant information on how genomic elements are coupled with regulatory proteins and functionally organized into hierarchical domains through their interactions. However, visualizing the time-dependent changes of such organization in individual cells remains challenging. Recent developments of CRISPR systems for site-specific fluorescent labeling of genomic loci have provided promising strategies for visualizing chromatin dynamics in live cells. However, there are several limiting factors, including background signals, off-target binding of CRISPR, and rapid photobleaching of the fluorophores, requiring a large number of target-bound CRISPR complexes to reliably distinguish the target-specific foci from the background. Various modifications have been engineered into the CRISPR system to enhance the signal-to-background ratio and signal longevity to detect target foci more reliably and efficiently, and to reduce the required target size. In this review, we comprehensively compare the performances of recently developed CRISPR designs for improved visualization of genomic loci in terms of the reliability of target detection, the ability to detect small repeat loci, and the allowed time of live tracking. Longer observation of genomic loci allows the detailed identification of the dynamic characteristics of chromatin. The diffusion properties of chromatin found in recent studies are reviewed, which provide suggestions for the underlying biological processes.

• Journal of Advanced Navigation Technology
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• v.23 no.3
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• pp.251-257
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• 2019

The configuration of ultra-long optical transmission link with dispersion management and optical phase conjugation is proposed. The whole transmission link consist of 80 km (single mode fiber span) ${\times}$ 56 fiber spans. The artificial distribution of single mode fibers' lengths and residual dispersions in fiber spans, which are gradually increased/decreased as the span number is increased, is adopted to compensate for the distorted wavelength division multiplexed channels. Since the compensation effect through the artificial distribution in the previous researches is expected to decrease as the number of fiber spans are increased, three-time repetition of the artificial distribution patterns at intervals of 9 fiber spans applied into the link with dispersion management and optical phase conjugation is proposed. From the simulation results, it is confirmed that the compensation in the link configured by the special distribution pattern among 4 proposed patterns is slightly improved than the link configured by the conventional method, which is designed by the repeat-less distribution pattern.

• Animal Systematics, Evolution and Diversity
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• v.36 no.4
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• pp.336-346
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• 2020

Mitochondrial genome is an important molecule for systematic and evolutionary studies in metazoans. The development of next-generation sequencing (NGS) technique has rapidly increased the number of mitogenome sequences. The process of generating mitochondrial genome based on NGS includes different steps, from DNA preparation, sequencing, assembly, and annotation. Despite the effort to improve sequencing, assembly, and annotation methods of mitogenome, the low quality and/or quantity sequence in the final map can still be generated through the work. Therefore, it is necessary to check and curate mitochondrial genome sequence after annotation for proofreading and feedback. In this study, we introduce the pipeline for sequencing and curation for mitogenome based on NGS. For this purpose, two mitogenome sequences of Dermatobranchus otome were sequenced by Illumina Miseq system with different amount of raw read data. Generated reads were targeted for assembly and annotation with commonly used programs. As abnormal repeat regions present in the mitogenomes after annotation, primers covering these regions were designed and conventional PCR followed by Sanger sequencing were performed to curate the mitogenome sequences. The obtained sequences were used to replace the abnormal region. Following the replacement, each mitochondrial genome was compared with the other as well as the sequences of close species available on the Genbank for confirmation. After curation, two mitogenomes of D. otome showed a typically circular molecule with 14,559 bp in size and contained 13 protein-coding genes, 22 tRNA genes, two rRNA genes. The phylogenetic tree revealed a close relationship between D. otome and Tritonia diomea. The finding of this study indicated the importance of caution and curation for the generation of mitogenome from NGS.

• Biomolecules & Therapeutics
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• v.30 no.4
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• pp.380-388
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• 2022

Snail is implicated in tumour growth and metastasis and is up-regulated in various human tumours. Although the role of Snails in epithelial-mesenchymal transition, which is particularly important in cancer metastasis, is well known, how they regulate tumour growth is poorly described. In this study, the possible molecular mechanisms of Snail in tumour growth were explored. Baculoviral inhibitor of apoptosis protein (IAP) repeat-containing protein 3 (BIRC3), a co-activator of cell proliferation during tumourigenesis, was identified as a Snail-binding protein via a yeast two-hybrid system. Since BIRC3 is important for cell survival, the effect of BIRC3 binding partner Snail on cell survival was investigated in ovarian cancer cell lines. Results revealed that Bax expression was activated, while the expression levels of anti-apoptotic proteins were markedly decreased by small interfering RNA (siRNA) specific for Snail (siSnail). siSnail, the binding partner of siBIRC3, activated the tumour suppressor function of p53 by promoting p53 protein stability. Conversely, BIRC3 could interact with Snail, for this reason, the possibility of BIRC3 involvement in EMT was investigated. BIRC3 overexpression resulted in a decreased expression of the epithelial marker and an increased expression of the mesenchymal markers. siSnail or siBIRC3 reduced the mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9. These results provide evidence that Snail promotes cell proliferation by interacting with BIRC3 and that BIRC3 might be involved in EMT via binding to Snail in ovarian cancer cells. Therefore, our results suggested the novel relevance of BIRC3, the binding partner of Snail, in ovarian cancer development.

• Molecules and Cells
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• v.45 no.8
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• pp.537-549
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• 2022

Preproenkephalin (PPE) is a precursor molecule for multiple endogenous opioid peptides Leu-enkephalin (ENK) and Met-ENK, which are involved in a wide variety of modulatory functions in the nervous system. Despite the functional importance of ENK in the brain, the effect of brain-derived factor(s) on PPE expression is unknown. We report the dual effect of neural epidermal growth factor (EGF)-like-like 2 (NELL2) on PPE gene expression. In cultured NIH3T3 cells, transfection of NELL2 expression vectors induced an inhibition of PPE transcription intracellularly, in parallel with downregulation of protein kinase C signaling pathways and extracellular signal-regulated kinase. Interestingly, these phenomena were reversed when synthetic NELL2 was administered extracellularly. The in vivo disruption of NELL2 synthesis resulted in an increase in PPE mRNA level in the rat brain, suggesting that the inhibitory action of intracellular NELL2 predominates the activation effect of extracellular NELL2 on PPE gene expression in the brain. Biochemical and molecular studies with mutant NELL2 structures further demonstrated the critical role of EGF-like repeat domains in NELL2 for regulation of PPE transcription. These are the first results to reveal the spatio-specific role of NELL2 in the homeostatic regulation of PPE gene expression.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.1
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• pp.39-48
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• 2023

Because a railway bridge is designed over a long period of time and covers a large site, it involves various environmental factors and uncertainties. For this reason, design changes often occur, even if the design was thoroughly reviewed in the initial design stage. In particular, design changes of large-scale facilities, such as railway bridges, consume significant time and cost, and it is extremely inefficient to repeat all the procedures each time. In this study, a technique that can improve the efficiency of learning after design change was developed by utilizing the learning result before design change through transfer learning among deep-learning algorithms. For analysis, scenarios were created, and a database was built using a previously developed railway bridge deep-learning-based prediction system. The proposed method results in similar accuracy when learning only 1000 data points in the new domain compared with the 8000 data points used for learning in the old domain before the design change. Moreover, it was confirmed that it has a faster convergence speed.

• Korean Journal of Radiology
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• v.25 no.10
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• pp.924-933
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• 2024

Objective: This study aimed to evaluate the performance of an integrated risk stratification system (RSS) based on ultrasound (US) RSSs, nodule size, and cytology subcategory for diagnosing malignancy in thyroid nodules initially identified as Bethesda category III on fine-needle aspiration. Materials and Methods: This retrospective study was conducted at two institutions and included consecutive patients with Bethesda category III nodules, and final diagnoses confirmed by repeat biopsy or surgery. A total of 320 Bethesda category III nodules (≥1 cm) from 309 patients (223 female and 86 male; mean age, 50.9 ± 12.0 years) were included. The malignancy risk of Bethesda category III nodules and predictors of malignancy were assessed according to US RSSs, nodule size, and cytology subcategory. The diagnostic performances of US-size cytology (USC) RSS and US RSS alone for malignancy were compared. Results: The intermediate or high suspicion US category independently increased the malignancy risk in all US RSSs (P ≤ 0.001). Large nodule size (≥3 cm) independently increased the malignancy risk of low- or intermediate suspicion US category nodules. Additionally, the atypia of undetermined significance cytology subcategory independently increased the malignancy risk of low suspicion US category nodules in most US RSSs. The area under the receiver operating characteristic curve of the USC RSSs was greater than that of the US RSSs alone (P < 0.048). Malignancy was not found in the very low risk category of USC RSS. Conclusion: The diagnostic performance of USC RSS for malignancy was superior to that of US RSS alone in Bethesda category III nodules. Malignancy can be ruled out in the very low-risk category of USC RSS.