• Journal of Applied Biological Chemistry
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• 제62권4호
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• pp.347-353
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• 2019

Stings of jellyfish, which frequently occur in a warm season, cause severe pain, inflammation and sometimes irreversible results such as the death. Harmful venoms from jellyfish, therefore, have been studied for finding the therapeutic agents to relieve pain or to neutralize toxic components. However, it is still unclear if and how jellyfish venom reveal neuronal toxicity even though pain induction seems to result from the activation of nociceptors such as nerve endings. In this study, using HT-22 cell line, we investigated neurotoxic effects of the venom of Chrysaora pacifica (CpV) which appears in South-East ocean of Korea. In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, CpV significantly reduced the viability of HT-22 cells in a dose-dependent manner. Additionally, in 2',7'-Dichlorofluorescin diacetate fluorescence test under the culture condition lacking dominant inflammatory factors, CpV remarkably increased the production of intracellular reactive oxygen species (ROS). Reduced responsive fluorescence to Rhodamine123 and increased expression of intracellular cytochrome c were also observed in HT-22 cells treated with CpV. These indicate that CpV-reduced viability of HT-22 cells may be due to the activation of apoptotic signalings mediated with oxidative stress and mitochondrial dysfunction. Furthermore, removing Ca²⁺ ion or adding N-acetyl-Lcystein remarkably blocked the CpV effect to reduce the viability of HT-22 cells. The findings in this study clearly demonstrate that CpV may activate Ca²⁺-mediated ROS signalings and mitochondrial dysfunction resulting in neuronal damage or death, and suggest that blocking Ca²⁺ pathway is a therapeutic approach to possibly block toxic effects of jellyfish venoms.

• Restorative Dentistry and Endodontics
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• 제31권3호
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• pp.161-168
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• 2006

근관형성을 하는 과정에서 기구가 파절될 가능성은 언제나 존재한다. File을 비롯하여 lentulo spiral, Gates-Glidden (GG) bur 또는 여타의 이물질도 근관 내에 남겨질 수 있다. 근래에는 수동 file에 비해 많은 장점을 가지고 있는 회전식 nickel-titanium (NiTi) file의 사용이 보편화 되고 있으나 기구의 높은 파절 경향이 문제가 되고 있다. NiTi file의 파절은 매우 빠른 시간내에 일어나고 예측이 불가능하며 임상가에게 정신적 stress를 가져다. 주기 때문에 임상가로 하여금 niTi file의 사용을 주저하게 만들기도 한다. 근관 내 기구가 파절되면 제거하는 것이 가장 최선의 해결 방법이다. 초음파 기기와 미세 현미경의 사용으로 파절된 기구의 제거율이 높아지고 있지만 제거하는 것이 쉬운 일만은 아니며 항상 제거가 가능한 것도 아니다. 그러므로 예방이 최선의 방법이라 할 수 있다. 본 증례에서는 근관 내 file이 파절된 경우에 있어 여러 가지 대처 방안에 대해 보고하고자 한다.

• FOCUS: LIFE SCIENCE
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• 제1호
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• pp.2.1-2.6
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• 2024

This article provides detailed instructions for the correct installation, maintenance, and troubleshooting of capillary gas chromatography (GC) columns. It emphasizes the importance of proper installation to ensure optimal performance and longevity of the column. The document covers various aspects such as column trimming, installation, conditioning, testing, storage, and ferrule selection. The installation process involves ensuring that the heated zones of the GC are cool before placing the column cage in the column oven. It is essential to avoid sharp bends or stress on the capillary column during installation and to connect the front end of the column into the GC inlet at the recommended insertion distance. The document also provides guidance on trimming the column, including the use of a ceramic wafer or capillary column cutter to achieve a clean, burr-free cut. For previously used columns, it recommends removing any capillary caps, positioning the nut and ferrule, and trimming 1-2 cm from the column. After installation, the column should be purged with carrier gas to remove any oxygen and avoid oxidizing the column. Conditioning the column involves ramping to the upper isothermal temperature limit and maintaining this temperature for a specified duration. It is crucial to maintain carrier gas flow during conditioning and not exceed the upper temperature limit of the column to avoid phase damage. The document also discusses testing column performance using a suitable method and performing a test injection to assess performance. It provides recommendations for column storage, including flame-sealing the capillary ends or using retention gaps for long-term storage. Additionally, it emphasizes the importance of routine maintenance and replacement of GC consumables to extend the column's lifetime. Ferrule selection is another important aspect covered in the article, with a variety of ferrule materials available for different applications. The characteristics of common ferrule options are presented in a table, including temperature limits, reusability, and suitability for specific detector types.

• 대한간호학회지
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• 제25권1호
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• pp.45-60
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• 1995

This study investigated the effects of education program conducted through individual home visit by CHPs, which was developed ,by the operational re-search technique for families of dementia patients. (Yeo Shin Hong et at,1994) The study was conducted in the form of a primary experimental design with 43 people as subjects, including dementia patients and family member in several Myon areas of Chungcheong Namdo between June 10 and August 20, 1994. The data was collected by questionnaires through the home visit by the CHPs. The results of study are as follows. 1. There is no difference in the quality of life between before and after the education program. 2. Role stress 'before the education program' was significantly different than 'after the education program'. 3. There was no difference in the feeling of burden between before and after the education program. 4. There was a significant difference in the abnormal behaviors of patients between before and after the education program. 5. The knowledge of dementia by the patient's family increased significantly after the education program, compared to that of 'before the education program'. 6. There was a significant difference in the attitude of family members toward the education program on dementia between before and after the education program. 7. The results of analysis on the coefficient relationship of various variables showed that the age of patients and family members have a significant correlation with role stress(p=.01). 8. In the subjective evaluation of family members on changes in actual nursing actions and the improvement of knowledge and technique in terms of daily living, (including abnormal behavior of patients, adjustment of environment for patients, activity programs for patients, communication technique with patients, ensuring the safety of patients, clothing, meals and elimination, 60-65% of family members responded that their knowledge had increased. As for improvement in techniques for each item, the technique for communication with patients showed the greatest improvement while the action program method for patients showed the least change. As for the nursing service provided to patients, most respondents showed a positive change. The specific items for which more than 80% respondents answered positively were as follows : recognizing the demand of patients, getting patients to do simple house works, talking softly and gently, removing dangerous things, preparing comfortable clothes that are easy to put on and take off, and limiting water consumption at night. As a result of study, the following suggestions can be made. The purpose of the study was to examine the effect of an education program developed and applied for dementia patients and family members in the community. This needs to be compared with a similar study conducted in the urban setting. In addition, a community service program (ex : nursing hem and shelter) including the application of the education program should be developed and the study done to investigate its effect.

• 한국치위생학회지
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• 제4권2호
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• pp.265-276
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• 2004

The purpose of this study,was to find out health status, characteristics related to working conditions, occurrence of low back pain and its related factors among dental hygienists working in dental clinics, and, thus, to provide basic information necessary to set up some plans for preventing the occurrence of low back pains and improving working conditions for dental hygienists. The data were collected from 310 dental hygienists working at dental clinics in Pusan and Kyungnam area, including Masan, Changwon, Jinhae and Jinju, with a self-administered questionnaire and were analyzed finally for 295 records with SPSS for Windows(7.52K) program. The results were as follows : Working condition was considered to be fair by 562% of dental hygienists were thought to threaten their health in the dental clinics. About seventy percent of dental hygienists worked over ten hours a day and 72.8% were standing while working over 7 hours, 65% considered working hours too long. Health status was thought be more than average for 82.3% while more than half perceived certain degree of stress frequently. The rate of complaining low back pains among dental hygienists was 87.8% with pains more than medium level for 39.3%. The variables significantly related to the degree of low back pains were height, uncomfortable postures, type of working postures and the height of working table, while some variables including height, hours of working on the feet, the presence of hazardous work environment, uncomfortable postures, the presence of hazardous works, the amount of working hours, type of working postures and work-associated stresses, were significantly related to the number of symptoms for low back pains. Multiple regression analysis showed that occurrence of low back pains was significantly influenced by 5 factors such as subjective status of health, work-related stresses, the degree of perception to work environment, the degree of perception to the amount of working hours and age. In conclusion, it might be necessary to manage working conditions effectively by reducing working hours, making good postures while working and removing stressful conditions in order to reduce risk factors for the occurrence of low back pains among dental hygienists. Therefore good working postures, assignment of appropriate rest time and some programs for early detection, care and education of low back pains should be provided for the dental hygienists.

• 한국산학기술학회논문지
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• 제16권8호
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• pp.5074-5081
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• 2015

플라스틱 제품의 휨을 개선하기 위한 방법은 사출성형 공정에서 일어나는 불균일한 냉각을 균일하게 만들어 플라스틱 제품의 잔류응력을 제거하는 방법이다. 본 연구는 균일한 냉각을 위하여 펠티에 소자를 사용하여 급속가열 냉각 장치를 개발하였다. 급속냉각 가열 장치(RCHD)를 제작하여 전통적인 수냉 장치(TWCD)방식과 급속냉각 가열 장치방식에 따른 휨을 비교 분석하였고, 비결정성 수지인 ABS 수지를 사용하였다. 사출성형 조건인 보압시간, 금형온도, 냉각시간, 보압에 따라 휨의 변화량을 측정 비교하였고, 비결정성 ABS 수지에서 급속가열 냉각 장치 냉각방식이 전통적인 수냉방식 보다 휨이 더 적게 발생하고, 위의 결과들로 보아 조금 더 균일하게 냉각되는 것을 알 수 있었다. ABS 폴리머의 분포 상태를 SEM 사진을 통해서 확인하였다. 전통적인 수냉방식은 폴리머의 분포상태가 조밀하게 분포되어 있고, 급속냉각 가열 방식은 전통적인 수냉방식 보다 넓게 분포되어 있었다. 이것은 냉각이 균일하게 이루어지고, 금형의 온도가 서서히 진행되면서 폴리머의 입자가 커지게 되는데, 이것은 내부응력이 줄어든 것을 의미한다.

• 지구물리와물리탐사
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• 제10권4호
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• pp.241-251
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• 2007

본격적인 지진관측 이래 최대 규모의 내륙 지진으로 기록된 오대산지진의 관측 스펙트럼을 이용하여, 점지진원 스펙트럼 모델의 지진원 크기 및 오차의 공간적인 특성을 평가하였다. 먼저 지진원 스펙트럼을 추정하기 위해, 최근까지 국내에 축적된 지진자료를 기반으로 비교적 상세하게 추정된 추계학적 지진동모델(Boore, 2003)의 지진파 전달, 부지특성(연관희, 2007)을 이용하여 관측 자료를 보정하였다. 추정된 오대산지진의 지진원 스펙트럼을 $1-f_c$(1개의 코너주파수) Brune의 ${\omega}^2$ 지진원모델에 적합한 결과, 기존에 제시된 지진규모-응력강하량 대표모델(연관희 등, 2006)에 의해 잘 예측되었으며, 오대산지진의 지진원 스펙트럼은 최근까지 한반도 인근에서 발생한 중규모 이상의 지진원 스펙트럼으로부터 추정된 $2-f_c$(2개의 코너주파수)의 경험적인 지진원모델에 보다 잘 부합되었다. 또한 일반적으로 무작위 잡음으로 취급되는 점지진원 지진파 스펙트럼 모델링 오차에 대해 방위각에 따른 방향성과 지역별 전달특성을 평가한 결과, 오차가 완전한 무작위 특성이 아님을 확인할 수 있었다. 이러한 모델링 오차의 방향성은 이론적으로 추정된 방사패턴과도 매우 유사한 관측된 방사패턴을 나타내었으며, 지역별로는 지질학적인 경계 혹은 지진파전달의 불연속적 특성과 밀접한 관계가 있는 것으로 판단되는 주파수별로 상이한 공간적인 분포 특성을 보여주었다.

• Steel and Composite Structures
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• 제46권1호
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• pp.53-73
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• 2023

Man-made structure materials like concrete usually contain inclusions. These inclusions affect the mechanical properties of concrete. In this investigation, the influence of inclusion length and inclination angle on three-dimensional failure mechanism of concrete under uniaxial compression were performed using experimental test and numerical simulation. Approach of acoustic emission were jointly used to analyze the damage and fracture process. Besides, by combining the stress-strain behavior, quantitative determination of the thresholds of crack stress were done. concrete specimens with dimensions of 120 mm × 150 mm × 100 mm were provided. One and two holes filled by gypsum are incorporated in concrete samples. To build the inclusion, firstly cylinder steel tube was pre-inserting into the concrete and removing them after the initial hardening of the specimen. Secondly, the gypsum was poured into the holes. Tensile strengths of concrete and gypsum were 2.45 MPa and 1.5 MPa, respectively. The angle bertween inclusions and axial loadind ary from 0 to 90 with increases of 30. The length of inclusion vary from 25 mm to 100 mm with increases of 25 mm. Diameter of the hole was 20 mm. Entirely 20 various models were examined under uniaxial test. Simultaneous with experimental tests, numerical simulation (Particle flow code in two dimension) were carried out on the numerical models containing the inclusions. The numerical model were calibrated firstly by experimental outputs and then failure behavior of models containing inclusions have been investigated. The angle bertween inclusions and axial loadind vary from 0 to 90 with increases of 15. The length of inclusion vary from 25 mm to 100 mm with increases of 25 mm. Entirely 32 various models were examined under uniaxial test. Loading rate was 0.05 mm/sec. The results indicated that when inclusion has occupied 100% of sample thickness, two tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. When inclusion has occupied 75% of sample thickness, four tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. When inclusions have occupied 50% and 25% of sample thickness, four tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. Also the inclusion was failed by one tensile crack. The compressive strength of samples decease with the decreases of the inclusions length, and inclusion angle had some effects on that. Failure of concrete is mostly due to the tensile crack. The behavior of crack, was affected by the inclusion length and inclusion number.

• Advances in aircraft and spacecraft science
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• 제11권1호
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• pp.83-103
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• 2024

Adhesive bonding is currently widely used in many industrial fields, particularly in the aeronautics sector. Despite its advantages over mechanical joints such as riveting and welding, adhesive bonding is mostly used for secondary structures due to its low peel strength; especially if it is simultaneously exposed to temperature and humidity; and often presence of bonding defects. In fact, during joint preparation, several types of defects can be introduced into the adhesive layer such as air bubbles, cavities, or cracks, which induce stress concentrations potentially leading to premature failure. Indeed, the presence of defects in the adhesive joint has a significant effect on adhesive stresses, which emphasizes the need for a good surface treatment. The research in this field is aimed at minimizing the stresses in the adhesive joint at its free edges by geometric modifications of the ovelapping part and/or by changing the nature of the substrates. In this study, the finite element method is used to describe the mechanical behavior of bonded joints. Thus, a three-dimensional model is made to analyze the effect of defects in the adhesive joint at areas of high stress concentrations. The analysis consists of estimating the different stresses in an adhesive joint between two 2024-T3 aluminum plates. Two types of single lap joints(SLJ) were analyzed: a standard SLJ and another modified by removing 0.2 mm of material from the thickness of one plate along the overlap length, taking into account several factors such as the applied load, shape, size and position of the defect. The obtained results clearly show that the presence of a bonding defect significantly affects stresses in the adhesive joint, which become important if the joint is subjected to a higher applied load. On the other hand, the geometric modification made to the plate considerably reduces the various stresses in the adhesive joint even in the presence of a bonding defect.

• 대한수의학회지
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• 제36권2호
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• pp.305-312
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• 1996

Cell volume regulatory mechanisms are usually disclosed by exposure of cell to anisotonic media. If a cell is suddenly exposed to hypotonic media, it swells initially like an osmometer but within minutes regains its original cell volume. This behavior has been labelled as regulatory cell volume decrease(RVD). RVD is believed to result from the loss of permeable ions through the membrane. In this study, we examined hypotonically induced changes in the membrance currents involved in RVD by using whole cell voltage clamp technique in the unfertilized hamster egg. At -40mV of the holding potential, the stationary current was maintained in the hamster egg exposed to isotonic solution composed of, mainly, 115mM NaCl and 40mM mannitol. Hypotonic solution was prepared by removing mannitol. Therefore, the concentrations of $Na^+$ and $Cl^-$ in this hypotonic media were the same as those in the isotonic solution. Following 30 to 60 sec after applying the hypotonic media to the egg, the inward current was evoked. This inward current was eliminated by $100{\mu}M$ 4-acetamido-4'-isothiocyanostil-bene-2,2'-disulfonic acid(SITS), an anion channel blocker, leaving the small outward current component. Further addition of 2mM $Ba^{2+}$, a broad $K^+$ channel blocker, completely abolished the small outward current left even in the presence of SITS during hypotonic stress. These results suggest that $K^+$ and $Cl^-$ move out of cells, resulting in RVD. To test the involvement of $Na^+$ in RVD, 20mM Na-isethionate was substituted for mannitol in isotonic media(135mM $Na^+$) and Na-isethionate (20mM) was freed the hypotonic solution. Only $Cl^-$ concentration in both isotonic and hypotonic media was kept constant at 115mM, whereas concentration of $Na^+$ was lowered in hypotonic solution to 115mM from 135mM in isotonic solution. Hypotonic medium induced the outward current in the egg equilibrated isotonically. This current was reduced by $100{\mu}M$ SITS but was augmented by 2 mM $Ba^{2+}$. In terms of RVD, these results imply that $Cl^-$ efflux is coupled with $K^+$, maybe for electroneutrality during hypotonic stress and/or with $Na^+$ via unknown transport mechanism(s). From the overall results, the hypotonic stress facilitates the movement of $Cl^-$ and $K^+$ out of the hamster egg to regain cellular volume with electroneutrality. If there exist a difference in $[Na^+]_0$ between isotonic and hypotonic solution, another transport mechanism concerned with $Na^+$ may, at least partly, participate in regulatory volume decrease.