• Title/Summary/Keyword: Release effect

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Effects of Tributyrin on Intestinal Energy Status, Antioxidative Capacity and Immune Response to Lipopolysaccharide Challenge in Broilers

  • Li, Jiaolong;Hou, Yongqing;Yi, Dan;Zhang, Jun;Wang, Lei;Qiu, Hongyi;Ding, Binying;Gong, Joshua
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.12
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    • pp.1784-1793
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    • 2015
  • This study was carried out to investigate the effects of tributyrin (TB) on the growth performance, pro-inflammatory cytokines, intestinal morphology, energy status, disaccharidase activity, and antioxidative capacity of broilers challenged with lipopolysaccharide (LPS). A total of 160 one-day-old Cobb broilers were allocated to 1 of 4 treatments, with 4 replicated pens per treatment and 10 birds per pen. The experiment consisted of a $2{\times}2$ factorial arrangements of treatments with TB supplementation (0 or 500 mg/kg) and LPS challenge (0 or $500{\mu}g/kg$ body weight [BW]). On days 22, 24, and 26 of the trial, broilers received an intraperitoneal administration of $500{\mu}g/kg$ BW LPS or saline. Dietary TB showed no effect on growth performance. However, LPS challenge decreased the average daily gain of broilers from day 22 to day 26 of the trial. Dietary TB supplementation inhibited the increase of interleukin-$1{\beta}$ (in the jejunum and ileum), interleukin-6 (in the duodenum and jejunum), and prostaglandin $E_2$ (in the duodenum) of LPS-challenged broilers. Similar inhibitory effects of TB in the activities of total nitric oxide synthase (in the ileum) and inducible nitric oxide synthase (in the jejunum) were also observed in birds challenged with LPS. Additionally, TB supplementation mitigated the decrease of ileal adenosine triphosphate, adenosine diphosphate and total adenine nucleotide and the reduction of jejunal catalase activity induced by LPS. Taken together, these results suggest that the TB supplementation was able to reduce the release of pro-inflammatory cytokines and improve the energy status and anti-oxidative capacity in the small intestine of LPS-challenged broilers.

Control Efficacy of Natural Enemies on Four Arthropod Pests found in Greenhouse Hot Pepper (시설재배 고추에 발생하는 4종의 주요 해충에 대한 천적이용 효과)

  • Kim, Jeong-Hwan;Byeon, Young-Woong;Choi, Man-Young;Ji, Chang-Woo;Heo, Su-Yeong;Park, Eun-Mi;Kang, Eun-Jin
    • Korean journal of applied entomology
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    • v.51 no.2
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    • pp.83-90
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    • 2012
  • The effect of natural enemies on four major pests of hot pepper was tested in greenhouses. The aphids were successfully controlled by introducing three Aphidius colemani-banker plants, and releasing 23.3 wasps per $m^2$ on April 16 and 23 wasps per $m^2$ in a greenhouse of 660 $m^2$. To control thrips, Orius laevigatus was released twice, 3.0 bugs per $m^2$ at a time(May $11^{th}$ and June $12^{th}$). The thrips population was controlled within 0.3 thrips per flower during the growing season. To control two species of mites, Tetranychus kanzawai and Polyphagotarsonemus latus, and the silverleaf whitefly, Bemisia tabaci, a total of 113.7 individuals of Amblyseius swirskii were released twice(May $11^{th}$ and $30^{th}$). The densities of B. tabaci and T. kanzawai were kept within 171.0 individuals/trap and 0.8 individual/leaf, respectively. P. latus was suppressed completely twelve days after release. The cost of the released natural enemies to control the four arthropod pests in this study was 420,000 Won per 660 $m^2$.

Storage properties of brown rice cultivar as affected by milling degree and storage conditions

  • Choi, Induck;Yoon, Mi-Ra;Kwak, Jieun;Chun, Areum;Jeon, Yong-Hee;Jeong, Tae-Wook;Kim, Sun-Lim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.262-262
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    • 2017
  • Brown rice contains a variety of nutritional and bio-functional components compared to milled rice. However, low consumer preference for steamed brown rice and short shelf life has been implicated as deterrents to the direct consumption of brown rice. This study was undertaken to determine the effect of degree of milling (DOM) on pasting and aging properties of brown rice cultivar. Short-grain (Japonica type) brown rice cv. Boseogchal (BSC), Baegjinju (BJJ), and Hiami (HIAM) were evaluated every month during 4 months storage. Rice cultivar were categorized into waxy (BSC), medium-waxy (BJJ), and non-waxy (HIAM) of which amylose contents were 6.81%, 10.06%, and 16.69~16.72%, respectively. Peak viscosity was the highest in BJJ followed by HIAM, and BSC, showing peak viscosity was lower at low temperature storage. At storage at room temperature from 60 to 120 days, the FFA content significantly increased in the rice samples with DOM1% followed by 3% and 5%, while not significant increase in DOM0% brown rice: at 120 days, the FFA ranged in 28.35~41.48, 111.99~130.06, 76.87~91.99, and 47.13~62.37 mg KOH/100g for DOM 0%, 1%, 3% and 5%, respectively. It indicated that the fat rancidity was the least in the order of DOM 0%, 5%, 3% and 1%. Storage at low temperature could reduce fat hydrolysis with lower FFA release, but rice grain with 1% MD also released more FFA compared to the other samples. It could be assumed that the most outer bran layer, approximately 1~3%, could provide a strong protection from fat rancidity. Pasting viscosity of rice samples also increased with increasing DOM rate. Results indicated that DOM was the most significant factor for lipid rancidity during storage, resulting in high FFA formation. It suggested that brown rice with DOM 0% and/or more than DOM 5% could provide the best shelf life.

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The change of core habitats of the cranes due to release of the civilian control zone; CCZ and construction disturbance (서식지 교란 및 민간인통제지역 해제에 의한 두루미와 재두루미의 핵심서식지 변화)

  • Yoo, Seunghwa;Jung, Hwayoung;Kim, Kyoungsoon;Yu, Dong Su;Kim, Namshin;Kim, Hwajung;Hur, Weehaeng;Kim, Jinhan;Lee, Kisup
    • Journal of Environmental Impact Assessment
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    • v.24 no.4
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    • pp.301-316
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    • 2015
  • The endangered species, The Red-crowned Crane and the White-naped Crane are vulnerable species to the disturbance for human beings. We examined the importance of CCZ for the cranes by comparing the crane's density in some CCZ-released areas, which are cranes' wintering site in Cheorwon. We also assessed influence of disturbance such as construction and greenhouses on core habitats of cranes. Our study results suggested that the construction and reclamation in the CCZ shrunk core habitat area while increasing core area of far from the construction and reclamation area. The CCZ has been set since March, 2011 and the number of greenhouse has rapidly increased after 2012. As the number and size of greenhouse in the area where designation of CCZ was cancelled increased, foraging area of cranes diminished. Although the area where designation of CCZ was cancelled seemed to have more human disturbance than CCZ, the foraging density of cranes did not decline and even that of white-naped cranes increased. This could be the influence of artificial food supply at their roosting site. In conclusion, if the area of CCZ decline continuously in the future, density of cranes would decrease.

Immunomodulatory Effects of an Extract from Pinus koraiensis Cone Peel in LP-BM5 Murine Leukemia Viruses-Induced Murine Acquired Immune Deficiency Syndrome (면역 결핍 동물모델에서 잣피 추출물의 면역조절 효과)

  • Kim, Seong Pil;Kwon, Han Ol;Ha, Yejin;Heo, Seok Hyun;Lee, Jeongmin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.9
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    • pp.1027-1034
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    • 2017
  • The immune system is a complex process within the body that protects against disease. Recently, many studies have attempted to discover immunomodulative compounds from natural sources. Pinus koraiensis (PK) cone shell is a by-product of PK. One of the major compounds of PK cone shell is dehydroabietic acid, which has bioactivity, including antiulcer and anti-inflammatory activities. Therefore, this study was performed to examine the immunomodulative effects of PK cone shell. The immunomodulatory effects of PK cone shell extracted with 20% ethanol (EtOH) in vivo were examined initially by measuring the natural killer (NK) cell activity, phagocytic activity, Th1/Th2 cytokines release, serum immunoglobulin, and T/B cell proliferation. The NK cell activity and phagocytic activity were increased significantly by a treatment with a 20% EtOH extract of PK cone shell. Th1 type cytokine and T cell proliferation increased and Th2 type cytokine, B cell proliferation and serum immunoglobulin A, G, and E decreased after a treatment with PK cone shell extract. The 20% EtOH extract of the PK cone shell normalized the unbalanced production of Th1/Th2 type cytokine. This suggests that a 20% EtOH extract of PK cone shell has great potential as a health food.

Identification of Muscle Proteins Related to Objective Meat Quality in Korean Native Black Pig

  • Hwang, I.H.;Park, B.Y.;Cho, S.H.;Kim, J.H.;Choi, Y.S.;Lee, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.11
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    • pp.1599-1607
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    • 2004
  • This study examined the effects of pre-slaughter fasting, chasing stress and chiller ageing on objective meat quality, and their relations to the proteome profile of longissimus muscle using 20 male Korean native black pigs. Treatments were composed of two levels of pre-slaughter feed withdrawal, two levels of pre-slaughter stress and four chiller ageing times. A 15 min chasing stress immediately prior to slaughter significantly (p<0.05) decreased detectable levels of $\mu$-calpain activity during rigor development and chiller ageing, but did not have any direct effect on objective meat quality. On the other hand, pigs fed until the morning of slaughter resulted in significantly (p<0.05) higher hunter L* value and cooking loss than those which received an 18 h feed withdrawal prior to slaughter. Cooking loss and hunter L* value were constant during 7 d of chiller ageing, followed by significant increases at 14 d. The fed animals showed a significantly (p<0.05) higher hunter a* value at both 3 and 7 d, while the other group maintained a stable redness for 7 d. WB-shear force was not affected by the pre-slaughter treatments, but had significant (p<0.05) linear reduction from 1 to 7 d. A gelbased proteome analysis was performed on selected animals for low and high hunter L* values at 1 d. Ten and five spots had greater than two-fold spot densities for the low and high hunter L* groups, respectively. The ten spots included chain A, deoxyribounclease I complex with actin, heat shock protein 27 kDa, a protein similar to cardiac $Ca^{2+}$ release channel, and myosin heavy chain, while the five spots included chain A aldehyde dehydrogenase, glycerol-3 phosphate dehydrogenase, and hemoglobin alpha chain. In general, feeding until the morning of slaughter resulted in more desirable meat color, but appeared to reduce palatability due to increased cooking loss. Proteome analysis demonstrated that various proteins were concomitantly involved in the determination of final meat color. The most noticeable observation in the current study was that various isoforms for a particular protein differed in degradation and/or expression rate depending on meat quality.

Design of a Stainless Steel Insert for Mechanical Joining of Long Fiber-reinforced Composite Structures (장섬유강화 복합재료 구조물의 기계적 접합을 위한 스테인레스 강 인서트 설계)

  • Lee, Sung-Woo;Chang, Seung-Hwan
    • Composites Research
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    • v.31 no.4
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    • pp.139-144
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    • 2018
  • Long Fiber-reinforced composites have advantages of excellent production efficiency and formability of complex shapes compared to conventional continuous fiber reinforced composite materials. However, if we need to make complicated composite shapes or to assemble parts made of different materials, a variety of joining methods are needed. In general, long fiber prepreg sheet (LFPS) contains mold release agent to facilitate demolding after thermoforming. Therefore, mechanical fastening is required in addition to the adhesive bonding to get proper joining strength. In this study, we proposed a stainless steel insert for co-cure bonding which cures LFPS and bonds the stainless steel insert through thermoforming process. The wing of the insert which is spread during the thermoforming process induces adhesion and mechanical wedging effect and serves as a hook to resist the pulling force. The burn-out method was used to confirm the unfolded state of the stainless steel insert wings inserted into the composite material. The static pull-out test was performed to quantitatively evaluate the joining strength. From these experimental results, the condition which guarantees the most appropriate joining strength was derived.

Development of Leg Stiffness Controllable Artificial Tendon Actuator (LeSATA®) Part I - Gait Analysis of the Metatarsophalangeal Joint Tilt Angles Soonhyuck - (하지강성 가변 인공건 액추에이터(LeSATA®)의 개발 Part I - Metatarsophalangeal Joint Tilt Angle의 보행분석 -)

  • Han, Gi-Bong;Eo, Eun-Kyung;Oh, Seung-Hyun;Lee, Soon-Hyuck;Kim, Cheol-Woong
    • Transactions of the KSME C: Technology and Education
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    • v.1 no.2
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    • pp.153-165
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    • 2013
  • The established gait analysis studies have regarded leg as one single spring. If we can design a knee-ankle actuating mechanism as a primary actuator for supporting knee extension, it might be possible to revolutionary store or release elastic strain energy, which is consumed during the gait cycle, and as a result leg stiffness is expected to increase. An ankle joint actuating mechanism that stores and releases the energy in ankle joint is expected to support and solve excessive artificial leg stiffness caused by the knee actuator (primary actuator) to a reasonable extent. If unnecessary kinematic energy is released with the artificial speed reduction control designed to prevent increase in gait speed caused by increase in time passed, it naturally brings question to the effectiveness of the actuator. As opposed to the already established studies, the authors are currently developing knee-ankle two actuator system under the concept of increasing lower limb stiffness by controlling the speed of gait in relative angular velocity of the two segments. Therefore, the author is convinced that compensatory mechanism caused by knee actuating must exist only in ankle joint. Ankle joint compensatory mechanism can be solved by reverse-examining the change in metatarso-phalangeal joint (MTPJ) tilt angle (${\theta}_1=0^{\circ}$, ${\theta}_2=17^{\circ}$, ${\theta}_3=30^{\circ}$) and the effect of change in gait speed on knee activity.

Arthroscopic Medial Plication using Pull-out Suture for the Treatment of Acute or Recurrent Patellar Dislocation - Technical Note - (급성 또는 재발성 슬개골 탈구의 치료에 있어서 견인 봉합술을 이용한 관절경적 내측 관절막 중첩술 - 수술 술기 -)

  • Ahn, Jin-Hwan;Kim, Jae-Hoon;Ha, Hae-Chan
    • Journal of the Korean Arthroscopy Society
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    • v.10 no.2
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    • pp.214-218
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    • 2006
  • Purpose: We describe a new technique of arthroscopic medial plication using pull-out suture with consideration of anatomical location of medial patellofemoral ligament for the treatment of acute or recurrent patellar dislocation. Operative technique: Under arthroscopic examination, sutures are passed through the medial capsule, at which medial patellofemoral ligament is located, from outside to inside of knee joint. Three guide wires are inserted from anterior surface of the patella to upper half of its medial border. Intraarticular portions of sutures are pulled out toward anterior surface of the patella through bony tunnels. Under appropriate tension, the sutures are tied after performing lateral retinacular release. Conclusion: As suturing medial patellofemoral ligament, this technique can maximize the effect of medial plication and can correct subluxation and tilt of the patella. It seems to be a minimally invasive, easy and effective method for the treatment of acute or recurrent patellar dislocation.

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Anti-obesity Effects of Extracts from Young Akebia quinata D. Leaves (어린 으름잎 추출물(Akebia quinata D. Leaves)의 항비만 효과)

  • Jeon, Yongseop;You, Yanghee;Jun, Woojin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.2
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    • pp.200-206
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    • 2014
  • We investigated the in vitro and in vivo anti-obesity effects of extracts from young Akebia quinata D. leaves, including hot water (AQH) and 80% ethanol (AQE) extracts. The inhibitory effects of AQH and AQE on lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Compared to control, lipid accumulation was significantly reduced by 18.3% with the treatment upon AQE at a concentration of $5{\mu}g/mL$. The levels of intracellular triglycerides and free glycerol were also reduced by 52.8% and 9.1% at the same concentration of AQE. The in vivo anti-obesity effect of AQE was evaluated in terms of body and white adipose tissue weights in ICR mice. Experimental groups were divided into the following five groups: normal diet (ND), high fat diet (HFD), high fat diet with 60 mg/kg/day of Orlistat (HFD-RF), high fat diet with 200 mg/kg/day of AQE (HFD-AL), and high fat diet with 600 mg/kg/day of AQE (HFD-AH). Feeding of HFD for eight weeks resulted in significant increases in body weight as well as weight gain compared to the ND group. HFD-AH group showed reduced body weight, weight gain, epididymal white adipose tissue weight, and perirenal white adipose weight as compared to the HFD group. These results indicate that AQE supplementation might have beneficial effects on anti-obesity by inhibiting lipid accumulation.