• 제목/요약/키워드: Related standard

검색결과 4,366건 처리시간 0.035초

산 및 열처리에 따른 오징어의 이화학적 특성변화 (Changes in Physicochemical Characteristics of Squid upon Acid and Heat Treatment)

  • 이혜영;김성호;김덕진
    • 한국식품영양과학회지
    • /
    • 제41권4호
    • /
    • pp.539-549
    • /
    • 2012
  • 본 연구는 오징어 가공식품개발의 기초자료확보를 위하여 산(acetic acid, 0~5%) 처리와 예비가열($55^{\circ}C$, $80^{\circ}C$) 후 열처리($100^{\circ}C$)에 따른 오징어의 이화학적 성분 변화를 조사하였다. 일반성분 함량은 수분이 약 73~78%, 조단백이 약 19~24%였으며 자숙 오징어의 일반성분 함량은 산 농도에 따라서 유의적인 차이가 있었다. 자숙 오징어의 모든 시료에서 주요 유리당은 ribose와 glucose이었으며, $55^{\circ}C$ 예비가열 시료는 다른 시료보다 glucose 함량이 낮은 것으로 나타났다. 총 유리당 함량은 표피 무제거 시료와 $80^{\circ}C$ 예비가열 시료순으로 높았고 $55^{\circ}C$ 예비가열 시료가 상대적으로 매우 낮았다. 모든 시료들의 유리아미노산 함량은 proline 함량이 가장 높았고 taurine, histidine 순으로 많이 함유되어 있었으며, 모든 자숙 오징어군에서 산 용액 처리농도에 따른 유의적인 영향을 보였다(p<0.05). 총 유리아미노산 함량은 $55^{\circ}C$ 예비가열 시료가 가장 높았고 $80^{\circ}C$ 예비가열, 표피 무제거 시료순으로 나타났다. 핵산관련물질은 모든 시료에서 inosine이 검출되지 않았고 ATP 함량이 가장 낮았다. $55^{\circ}C$$80^{\circ}C$ 예비가열 시료는 hypoxanthine 함량이 가장 높고 AMP와 IMP 함량이 비슷한 수치를 보였으나 표피 무제거 시료는 IMP 함량이 표피제거 시료보다 높았다. 지방산은 포화지방산이 약 40%로 palmitic acid 함량이 매우 높았고 불포화지방산은 약 60%로 EPA와 DHA 함량이 높았다. 지방산중 DHA 함량이 가장 높고 palmitic acid, EPA 순으로 나타났으며 이 세 가지가 총 지방산의 약 85%를 차지하고 있었다. 지방산은 산 처리와 가열 방법에 따른 뚜렷한 차이가 나타나지 않았다. 무기질의 함량은 인의 함량이 가장 높았고 칼륨, 나트륨, 마그네슘, 칼슘 순이었으며 예비가열 온도 또는 산 용액 농도에 따른 유의적인 영향을 받았다. 그리고 중금속류인 카드뮴, 납, 비소는 미량으로 나타나 식품 기준치보다 낮은 수준이었다.

설비공학 분야의 최근 연구 동향 : 2013년 학회지 논문에 대한 종합적 고찰 (Recent Progress in Air-Conditioning and Refrigeration Research : A Review of Papers Published in the Korean Journal of Air-Conditioning and Refrigeration Engineering in 2013)

  • 이대영;김사량;김현정;김동선;박준석;임병찬
    • 설비공학논문집
    • /
    • 제26권12호
    • /
    • pp.605-619
    • /
    • 2014
  • This article reviews the papers published in the Korean Journal of Air-Conditioning and Refrigeration Engineering during 2013. It is intended to understand the status of current research in the areas of heating, cooling, ventilation, sanitation, and indoor environments of buildings and plant facilities. Conclusions are as follows. (1) The research works on the thermal and fluid engineering have been reviewed as groups of fluid machinery, pipes and relative parts including orifices, dampers and ducts, fuel cells and power plants, cooling and air-conditioning, heat and mass transfer, two phase flow, and the flow around buildings and structures. Research issues dealing with home appliances, flows around buildings, nuclear power plant, and manufacturing processes are newly added in thermal and fluid engineering research area. (2) Research works on heat transfer area have been reviewed in the categories of heat transfer characteristics, pool boiling and condensing heat transfer and industrial heat exchangers. Researches on heat transfer characteristics included the results for general analytical model for desiccant wheels, the effects of water absorption on the thermal conductivity of insulation materials, thermal properties of Octadecane/xGnP shape-stabilized phase change materials and $CO_2$ and $CO_2$-Hydrate mixture, effect of ground source heat pump system, the heat flux meter location for the performance test of a refrigerator vacuum insulation panel, a parallel flow evaporator for a heat pump dryer, the condensation risk assessment of vacuum multi-layer glass and triple glass, optimization of a forced convection type PCM refrigeration module, surface temperature sensor using fluorescent nanoporous thin film. In the area of pool boiling and condensing heat transfer, researches on ammonia inside horizontal smooth small tube, R1234yf on various enhanced surfaces, HFC32/HFC152a on a plain surface, spray cooling up to critical heat flux on a low-fin enhanced surface were actively carried out. In the area of industrial heat exchangers, researches on a fin tube type adsorber, the mass-transfer kinetics of a fin-tube-type adsorption bed, fin-and-tube heat exchangers having sine wave fins and oval tubes, louvered fin heat exchanger were performed. (3) In the field of refrigeration, studies are categorized into three groups namely refrigeration cycle, refrigerant and modeling and control. In the category of refrigeration cycle, studies were focused on the enhancement or optimization of experimental or commercial systems including a R410a VRF(Various Refrigerant Flow) heat pump, a R134a 2-stage screw heat pump and a R134a double-heat source automotive air-conditioner system. In the category of refrigerant, studies were carried out for the application of alternative refrigerants or refrigeration technologies including $CO_2$ water heaters, a R1234yf automotive air-conditioner, a R436b water cooler and a thermoelectric refrigerator. In the category of modeling and control, theoretical and experimental studies were carried out to predict the performance of various thermal and control systems including the long-term energy analysis of a geo-thermal heat pump system coupled to cast-in-place energy piles, the dynamic simulation of a water heater-coupled hybrid heat pump and the numerical simulation of an integral optimum regulating controller for a system heat pump. (4) In building mechanical system research fields, twenty one studies were conducted to achieve effective design of the mechanical systems, and also to maximize the energy efficiency of buildings. The topics of the studies included heating and cooling, HVAC system, ventilation, and renewable energies in the buildings. Proposed designs, performance tests using numerical methods and experiments provide useful information and key data which can improve the energy efficiency of the buildings. (5) The field of architectural environment is mostly focused on indoor environment and building energy. The main researches of indoor environment are related to infiltration, ventilation, leak flow and airtightness performance in residential building. The subjects of building energy are worked on energy saving, operation method and optimum operation of building energy systems. The remained studies are related to the special facility such as cleanroom, internet data center and biosafety laboratory. water supply and drain system, defining standard input variables of BIM (Building Information Modeling) for facility management system, estimating capability and providing operation guidelines of subway station as shelter for refuge and evaluation of pollutant emissions from furniture-like products.

정신과 간호 실습에 대한 간호 대학생과 간호학교 학생들의 태도 비교 연구 (A Comparative Study on Attitude of the Collegiate an4 Non-Collegiate Nursing Students toward Their Clinical Affiliation in a Mental Hospital)

  • 김소야자
    • 대한간호학회지
    • /
    • 제4권2호
    • /
    • pp.17-31
    • /
    • 1974
  • Today, over seventy five percent of nursing in Korea provide a psychiatric experience in the basic curriculum. The psychiatric affiliation presents numerous major problems of adjustment to the student. The Importance of positive attitude toward the nursing care of psychiatric patients is recognized by the nursing profession. I have fined out the unfavorable attitude of non collegiate nursing students toward psychiatric nursing affiliation by previous research. This study was undertaken in response to a felt need to explore the use of several devices which might yield information about attitudes toward psychiatric nursing as a basis for future planning of the program offered at a selected hospital. This study is designed to meet the following objectives; (1) In order to find out the expressed attitudes of fifty·three collegiate nursing students toward their psychiatric affiliation. (2) To compare responses given by selected group of collegiate and non collegiate nursing students to same questionnaire (3) To determine the relationship between the attitudes of nursing students toward psychiatric nursing and the type of instructions where experience was obtained. A questionnaire, a Korean translation of the "Psychiatric Nursing Attitude Questionnaire" by Moldered Elizabeth fletcher, was administered to fifty-three collegiate nursing students who had completed a four-week psychiatric affiliation in a S hospital psychiatric ward during May 7, 1973 to Dec. 16, 1973. - The questionnaire of 100 statements was administered in the following way; (1) Part Ⅰ, Preconceptions, was, given in individual conferences with each subject, during the first few days of their affiliation, and again during the final week of affiliation. The responses to Part I were oral. (2) Part Ⅱ, Expectations, Part Ⅲ, Personal Relations, Part Ⅳ, Personal Feelings, and Part V, Attitudes and Activities of Patients were given to all of the subjects in a group meeting during the second week of the affiliation, and again, during the fourth week at the termination of the affiliation. Responses to Parts Ⅱ, Ⅲ, Ⅳ·, and V, were written. Each of the 100 statements of the questionnaire was considered to be either Positive or Negative. A favorable response was assigned the positive value of 1 and an unfavorable response was assigned the Negative value of O. The coefficient of correlation was computed between the two sets of scores for the fifty-three nursing students, The mean score, the standard deviation, and the differences in the means on each of the five parts of the questionnaire were computed and the relationships calculated by at-test. The results of the study were as follows; 1. There was no significant correlation between the two sets of the scores for the fifty-three nursing students during the four-week psychiatric affiliation. (r= 0.36) 2. There was no significant difference in the mean scores between the first and final tests for any of the questionnaire. 3. The Part Ⅰ, Preconceptions, data indicated collegiate nursing students have positive attitudes in preconceptions than non collegiate nursing students and preconceptions toward the psychiatric affiliation which affect their psychiatric nursing experience. 4. The Part Ⅱ, Expectations, data indicated more appropriate expectations of collegiate nursing students related to pre psychiatric affiliation orientation and sufficient theory learning than non-collegiate nursing students. 5. The Part Ⅲ, Personal relations, data indicated some students have negative attitudes in personal relations with normal people in respect to psychological security and social responsibilities. 6. The Part Ⅳ, Personal feelings, data indicated nursing students have psychological insecurity & inappropriateness. 7. The Part V, Attitudes and activities of patients, data indicated collegiate nursing students have more positive attitudes to the psychotic behavior of certain situations due to sufficient theory learning. 8. The data indicated collegiate·nursing students have more positive attitude than non-collegiate nursing students. 5. The Part Ⅲ, Personal relations, data indicated some students have negative attitudes in personal relations with normal people in respect to psychological security and social responsibilities. 6. The Part Ⅳ, Personal feelings, data indicated nursing students have psychological insecurity & inappropriateness. 7. The Part V, Attitudes and activities of patients, data indicated collegiate nursing students have more positive attitudes to the psychotic behavior of certain situations due to sufficient theory learning. 8. The data indicated collegiate·nursing students have more positive attitude than non-collegiate nursing students through psychiatric affiliation.

  • PDF

일부지역(一部地域) 노인(老人)들의 주체적(主體的) 건강수준(健康水準)에 영향을 미치는 IADL에 관한 연구(硏究) (A Study on the IADL Affecting Subjective Health Index of the Aged in Some Area)

  • 김근조;박흥기;권혁수;배수찬
    • 대한정형도수물리치료학회지
    • /
    • 제7권1호
    • /
    • pp.29-50
    • /
    • 2001
  • This research have been made to define how the IADL (Instrumental Activity of Daily Living) performance and the subjective health index of the Aged are affected by their residential circumstance, gender and age, and how deeply these two factors are related and interact. For the period of June 1 to July 31, 2000, we had conducted a questionnaire and direct interview with 693 persons over age sixty-five (65) in Daejon and in other adjacent area, grouping into three different residential types The Aged living at home, The Aged living at welfare facilities and The Aged living alone, and studied on how the IADL performance and the subjective health index of the Aged are influenced and interact as per their characteristics, daily activity and mentality. We had analyzed all the data obtained through this research by the method of : - analysis of frequency as per specific factors by SPSS 1O.0/PC+, - $x^2$ test, - t-test, - ANOVA, - multiple regression analysis by factors. The research concludes followings : a. It appears that the three (3) factors such as gender, age and residential circumstance of the Aged deeply affect the IADL performance and subjective health index of the Aged. (p<0.01) b. With regard to IADL performance of the Aged by the gender, it was analyzed that the female-Aged gains 23.8 point on average, which shows the performance of the female-Aged is less independent. (p<0.01). In addition, it was also found that the IADL performance is becoming less and less independent following their age increasing. In analyzing IADL performance by the residential type, it appears that the Aged living at welfare facilities gains the lowest 21.5 points and is least independent. It was also found that the Aged living at welfare facilities need some assistances from others for their performing IADL. (20-24 point) (p<0.001) c. With regard to the subjective health index of the all-Aged participated in this research, the analysis indicates 8.8 point and this is considered as a point of general standard (7-10 point). In analyzing this index by the gender, the female-Aged gains only 8.6 point which explains a lot of female-Aged consider they are not really healthy. (p<0.001) In analyzing this index by the residential type, the Aged living at welfare facilities and the Aged living alone gain the comparatively lower point, - respectively 8.4 point for the Aged living at welfare facilities and 8.8 point for the Aged living alone. The Aged living at these two residential types express they are obviously in a bad situation of health. (p<0.001) d. With regard to the factors affecting the IADL performance and the subjective health index of the Aged, it was analyzed that the IADL performance can largely be affected by the factors such as depression, frequency of going-out and age rising, and that the subject health index can also deeply be affected by depression, pain and by how much they are satisfied with their current living conditions. e. It was analyzed that the interacting between the IADL performance and the subjective health index is not that strong but even weak. As a result, we were able to conclude that the IADL performance is less independent in case of the female-Aged, the Aged living at welfare facilities, and following the age rising. As for the subjective health state the Aged themselves are aware of, we concluded that the female-Aged, the Aged living at welfare facilities and the Aged living alone, are more critical about their health. From this research, we were able to realize that, when the OMT (Orthopedic Manual Therapy) needed, the physical therapists are really required to have a correct and cautious understanding of the situation in which the aged persons are, and take care of them with more concerns and more improved treatment.

  • PDF

도시부도로에서의 하이패스 교통자료 특성분석 및 정보가공방안 (An Analysis into the Characteristics of the High-pass Transportation Data and Information Processing Measures on Urban Roads)

  • 정민철;김영찬;김동효
    • 한국ITS학회 논문지
    • /
    • 제10권6호
    • /
    • pp.74-83
    • /
    • 2011
  • 하이패스 교통정보시스템은 프로브 차량을 이용하여 직접 구간정보를 수집함으로써, 보다 신뢰성 높은 정보를 운전자에게 제공할 수 있다. 그러나 프로브 차량의 운행상황과 특성, 그리고 통계처리 방법 등이 정보의 신뢰성에 영향을 미치며 특히, 도시부도로에서는 구간통행시간이 신호 지체시간 경험 유무에 의해 크게 영향을 받기 때문에, 수집되는 개별 프로브 데이터 간 많은 편차가 발생하게 된다. 따라서 구간정보의 신뢰도 제고를 위한 다각적인 방면에서의 연구가 필요하다. 그러나 하이패스 정보제공과 관련한 선행연구는, 주로 연속류 특성을 가지는 고속도로 구간을 대상으로 이루어져, 이를 단속류 교통 특성을 가지는 도시부도로에 적용시키기에 한계가 있었다. 따라서 본 연구에서는 도시부도로에서의 하이패스 교통자료 특성을 분석하고 적절한 가공방안을 마련하는데 의의를 두고자 하였다. 시공간도를 이용하여 RSE로부터 수집하는 도시부도로의 하이패스 자료 특성을 분석한 결과, 수집자료는 종료노드의 신호주기를 주기로 하여 도착 차량의 신호 대기 등에 따라 일정한 패턴을 나타내는 것을 알 수 있었다. 또한, 신호대기 횟수와 대기시간에 따라 수집데이터의 편차가 발생하는데, 혼잡 상황보다 비혼잡 상황에서 편차가 크게 나타났다. 이는 혼잡상황에서는 신호대기 횟수가 많아지면서, 편차가 일정부분 상쇄되기 때문인 것으로 분석되었다. 이러한 도시부도로의 하이패스 수집자료는 신호대기에 의한 교통특성을 반영하기 위해 평균값을 대표값으로 사용하는 것이 적절하며, 신호 및 도로 특성에 따라 지정체 판단기준을 조절할 필요가 있는 것으로 분석되었다. 본 연구에서 도출한 결과가 도시부도로에서의 하이패스 정보의 신뢰성을 향상 시킬 수 있는 초석이 되길 기대한다.

K-IFRS 도입이 영업권손상차손 인식의 적시성에 미친 영향 (The Effect of K-IFRS Adoption on Goodwill Impariment Timeliness)

  • 백정한;최종서
    • 경영과정보연구
    • /
    • 제35권1호
    • /
    • pp.51-68
    • /
    • 2016
  • 본 연구는 K-IFRS 도입과 함께 기존의 상각규정을 폐지하고 매기간 손상검사를 실시하도록 요구하는 영업권 회계변경이 영업권손상차손 인식의 적시성에 미친 영향을 분석하고자 수행되었다. 원칙중심기준인 K-IFRS는 회계정보의 목적적합성을 강화하고 적시성 있는 정보를 전달하기 위해 공정가치평가를 확대하였으며 동일한 맥락에서 영업권과 같은 '비한정내용연수의 무형자산'에 대한 손상검사를 1년에 1회 이상 수행하도록 요구하였다. 그러나 영업권 회계변경의 효과를 분석한 선행연구들은 새로운 손상검사절차가 경영자들의 재량에 의존하고 있어 영업권손상차손의 인식이 적시성 있게 수행되기 보다는 기회주의적으로 이용되고 있음을 지적하고 있다. 하지만 선행연구들은 개별 기업에서 관찰될 수 있는 손상징후를 고려하지 못한 채 회계변경 전 후의 영업권 감액 빈도와 크기에만 초점을 맞추어 분석하고 있어 그 결과에 편의(bias)를 포함할 수 있다. 본 연구는 이러한 선행연구들의 한계를 극복하기 위해 Ramanna and Watts(2012)의 손상징후에 대한 정의를 활용하여 새로운 손상차손절차가 적시성 있는 회계정보의 전달이라는 목적을 달성하고 있는지 검증하고자 하였다. 2008년부터 2011년까지 상장되어 있는 기업 중 영업권을 보유한 947개 기업-연도 표본을 대상으로 분석을 실시한 결과 영업권손상차손 인식의 적시성은 손상차손 인식여부를 더미변수의 형태로 이용한 로지스틱 회귀분석과 연속변수의 형태로 이용한 다변량 회귀분석 모두에서 K-IFRS 이전에 비해 이후 기간에 감소된 것으로 나타났다. 분석결과를 보다 강건하게 하기 위해 또 다른 영업권 손상징후를 이용하여 동일한 분석을 재차 수행한 결과도 일관된 모습으로 조사되었다. 이러한 결과는 회계기준 제정기관들이 영업권 회계기준 변경을 통해 보다 적시성있고 목적 적합한 회계수치를 작성하도록 의도하였던 것과 달리 경영자들이 그들에게 주어진 재량권을 악용하여 영업권손상차손 인식시기를 조정함으로써 영업권손상차손 인식의 적시성이 감소하였음을 보여주는 결과이다. 본 연구는 영업권손상인식의 적시성 변화를 분석한 선행연구들의 분석방법에서 나타날 수 있는 편의(bias)를 감소시킬 뿐만 아니라 영업권손상에 대한 징후를 고려한 분석을 실시하여 회계변경으로 인한 손상차손인식의 적시성 변화를 보다 직접적으로 분석하였다는 점에서 의의가 있다.

  • PDF

국소 진행된 비소세포 폐암에서 복합요법과 단일요법의 비교 (Comparison of Single vs Combined Modality Treatment in Locally Advanced Non-Small Cell Lung Cancer)

  • 김애경;정성수;신경상;박상기;조혜정;이종진;서지원;김주옥;김선영
    • Tuberculosis and Respiratory Diseases
    • /
    • 제42권4호
    • /
    • pp.502-512
    • /
    • 1995
  • 연구배경: 최근까지 국소 진행된 비소세포 폐암의 치료율을 높이려는 여러 노력들이 있어왔지만 향상은 보이지 않는 설정에서 최근의 유도 화학요법 및 다각적 병합요법의 치료결과와 본 병원의 예를 봄으로 반응률 및 치료 성적을 비교하고 생존에 영향을 미치는 제반 요소들과의 상호 관계에 대해 조사하여 보고하는 바이다. 방법: stage III로 진단된 비소세포 폐암환자 총 67 명을 각각의 치료별로 분류하여 화학치료만 받은 군은 A 군이라 칭하였고, 화학 치료및 방사선 치료를 받은 군은 B군, 수술과 화학 치료를 받은 군은 C군, 비 치료군으로는 D군으로 명하여 이들 군간의 반응률 및 치료 성적을 상호 비교하였고 생존에 영향을 미칠 여러 제반요소들과의 상관관계를 알아 보았다. 결과: 1) 화학 치료만 받은 군은 27 명, 화학 치료 및 방사선 치료를 같이 받은 군은 18 명, 수술 및 화학 치료를 받은 군은 15 명, 비 치료군은 7 명으로 각 군간의 나이, 운동능력, 혈액형, 조직학적, 성별, 흡연력의 분포에 있어서 차이가 없었다(p>0.05). 2) 화학 치료만 받은 군과 방사선 치료도 병합하여 받은 군의 총 반응률은 47%으로 A군의 경우가 10%, B군이 11%를 차지하였으며 이들 간에 의미있는 반응율의 차이는 없었다(p=0.97). 3) 나이, 성별, 운동능력, 혈액형, 조직학적 차이, 흡연력의 치아가 반응률에 영향을 미치지는 않았다(p>0.05). 4) 치료별 A, B, C, D 각 군에 따론 생존 기간은 8.6개월, 13.4개월, 19.2개월, 5.4 개월로 의미있는 차이를 보였다(p=0.003). 비치료군보다는 치료군이, 화학 치료로 단독 치료만 받은 군보다는 병합 치료를 받은 군이 오랜 생존 기간을 보였다. 5) 치료시 생존기간에 영향을 미칠 요소라는 운동능력과 진단 당시 임상병기로 나타났으나 그 외 나이, 성별, 혈액형, 조직학적 분류, 흡연력이 의미있는 차이를 가져오지는 않았다. 결론: 국소 진행된 비소세포 폐암환자에서 복합 화학요법을 포함하여 수술 및 방사선 치료와의 병행은 화학요법 단독으로 치료한 군이든지 혹을 비치료군에 비해 월등하게 생존 기간의 향상을 가져옴으로 좋은 적응증을 가진 환자들을 선택하여 효과적인 화학 치료 및 다른 치료법을 병행하여야 할 것으로 사료된다. 하지만 이러한 여러 치료법들이 표준 치료로 정립되기 위해서는 보다 충분한 증례에서 추후연구가 필요하리라 여겨지는 바이다.

  • PDF

Improving Corsican pine somatic embryo maturation: comparison of somatic and zygotic embryo morphology and germination

  • Wtpsk, Senarath;Shaw, D.S.;Lee, Kui-Jae;Lee, Wang-Hyu
    • 한국자원식물학회:학술대회논문집
    • /
    • 한국자원식물학회 2003년도 춘계 학술발표대회
    • /
    • pp.61-62
    • /
    • 2003
  • Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022$^{\circ}$C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of either germination medium ($\frac{2}{1}$strength Murashige and Skoog (1962) salts with 2.5 g/l activated charcoal) or same medium with copper sulphate adjusted to 0.25 mg/1 to compensate for copper adsorption by activated carbon. 2% and 4% maltose was substituted by 7.5% and 13% PEG respectively to improve the yield of the embryos. Substitution of' maltose with PEG was clearly beneficial to embryo development. When 2% of the maltose was replaced with 7.5% PEG, many embryos developed to large bullet-shaped embryos. At latter stages of development most embryos callused and stopped development. A few short, barrel-shaped cotyledonary embryos formed that were covered by callus on the sides and base. When 4% of the maltose was removed and substituted with 13% PEG, the embryos developed further, emerging from the callus and increasing yield slightly. Microscopic examination of the cultures showed differing morphologies, varying from mostly single cells or clumps to well-formed somatic embryos that resembled early zygotic embryos only liquid cultures with organized early-stag. A procedure for converting and acclimating germinants to growth in soil and greenhouse conditions is also tested. Seedling conversion and growth were highly related to the quality of the germinant at the time of planting. Germinants with larger shoots, longer, straighter hypocotyls and longer roots performed best. When mature zygotic embryos germinate the root emerges, before or coincident with the shoot. In contrast, somatic embryos germinate in reverse sequence, with the cotyledons greening first, then shoot emergence and then, much later, if at all, the appearance of the root. Somatic seedlings, produced from the maturation medium, showed 100% survival when planted in a field setting. Somatic seedlings showed normal yearly growth relative to standard seedlings from natural seed.

  • PDF

Mucin 분비에 영향을 미치는 Metalloproteinase (Metalloproteinase Plays a Role in Mucin Secretion)

  • 오연목;최희진;심태선;이상도;김우성;김동순
    • Tuberculosis and Respiratory Diseases
    • /
    • 제56권3호
    • /
    • pp.289-296
    • /
    • 2004
  • 연구배경 : 기도 질환에서 점액이 과량 분비되는 경우 환자에게 불편함을 줄뿐만 아니라 기도 질환 예후에도 나쁜 영향을 미친다. 그러나, 기도 질환에서 점액이 과량 분비되는 것을 효과적으로 막는 방법이 없다. 점액의 성분 중 mucin은 당화 단백질로서 점액이 점성을 띄게 만드는 주요 성분이다. 본 연구를 통해서 mucin 분비 기전에 proteinase가 관여하는지 확인하고 만일 proteinase가 mucin 분비기전에 관여 한다면 어느 proteinase가 그런 역할을 하는지 확인하고자 하였다. 방 법 : (1) mucin 분비 억제 실험 군 특이적 proteinase 억제제를 사용하여 어느 군에 속하는 proteinase가 mucin 분비를 억제하는지 mucin을 생산하는 폐 세포주인 Calu-3를 이용하여 알아보았다. 군 특이적 proteinase 억제제로 PMSF(phenylmethylsulfonyl fluoride, serine proteinase inhibitor), E-64(cysteine proteinase inhibitor), Pepstatin(aspartic proteinase inhibitor), 1,10-Phenanthroline(metalloproteinase inhibitor)를사용하였다. 군 특이적 억제제를 Calu-3에 24시간동안 처리하여 분비된 mucin양을 enzyme linked immunoabsorbant assay(MUC5AC)로 정량하였고 그 결과를 대조군과 비교하였다. (2) Mucin 분비 자극 실험 Metalloproteinase 중에서 기도 질환 발병과 관련 있다고 알려진 matrix metalloproteinase-9 (MMP-9), MMP-12 그리고 TNF-alpha converting enzyme(TACE)를 Calu-3에 24시간 처리하여 분비된 mucin양을 enzyme linked immunoabsorbant assay (MUC5AC)로 정량하였고 그 결과를 대조군과 비교하였다. 결 과 : (1) 군 특이적 proteinase 억제제인 PMSF($10^{-4}M$), E-64($10^{-4}M$), Pepstatin($10^{-6}M$), 1,10-Phenanthroline($10^{-4}M$)는 MUC5AC 분비를 각각 $1{\pm}4.9%$(평균${\pm}$표준오차; 대조군과 비교 시 P=1.0), $-6{\pm}3.9%$ (P=0.34), $-13{\pm}9.7%$(P=0.34), $41{\pm}8.2%$(P=0.03) 감소시켰다(실험 회수 4번). (2) MMP-9(250ng/ml), MMP-12(100ng/ml), TACE(200ng/ml)에 의한 MUC5AC 분비량은 대조군에 비하여 각각 $103{\pm}6%$(P=0.39), $102{\pm}8%$(P=1.0), $107{\pm}13%$(P=0.39)이었다(실험 회수 6번). 결 론 : mucin 분비 기전에 metalloproteinase가 관여함을 시사하지만 MMP-9, MMP-12, TACE는 in vitro 모델에서 mucin 분비에 영향을 미치지 않았다.

아동의 심리발달과 도서선호의 변화 (A Study on the Relationship of Children's Development and Book Preference)

  • 한윤옥
    • 한국문헌정보학회지
    • /
    • 제17권
    • /
    • pp.105-159
    • /
    • 1989
  • The purpose of this study is (a) to identify physical and content elements of children's books that seem to interest second and fifth graders to read, and (b) to observe how the influence of those elements on children's reading interest shifts as children develop. For the purpose of this study, children's books are classified into two groups: 'books of low preference' and 'books of high preference' as determined by second graders, and fifth graders' library circulation record in the library chosen as the sample for the present study. The physical and content elements of the books belonging to these two groups are analyzed and compared. Main findings and conclusions made in this study are summarised as follows: (1) The physical elements of children's books which have significant relationship with second graders' reading preference are illustration quantity, size of types, space between lines, and size of the book. (2) The content elements of children's books which have significant relationship with second graders' reading preference are the readability level of sentences, and the sex of heroes. (3) The physical elements of children's books which have significant relationship with fifth graders' reading preference are illustration quantity, illustration styles, and size of the book. (4) The content elements of children's books which have significant relationship with fifth graders' reading preference are the readability level, the sex of heroes, subject matters, themes and the environmental settings as the background of stories. (5) Reading preference of the pupils at the second and fifth grades are influenced by the quantity of illustration. Second grade pupils prefer reading books which contain a larger quantity of illustrations, whereas 5th grade pupils prefer books which contain less quantity of illustrations. (6) The relationship between second graders' reading preference and the illustration style are rejected at the $10\%$ significance level. A strong relationship, however, appears to exist between 5th graders' reading preference and the illustration style. The 5th grade pupils prefer more realistic illustrations, while cartoons and semi-abstract drawings do not appeal to them. (7) The type size, and space between lines which have had great influence on the book preference of 2nd grade pupils influence little on the 5th grade pupils book preference. (8) Second grade pupils prefer the book of a small Size, whereas 5th grade pupils whose reading taste has developed at a primary stage prefer the book of a large size beyond a certain standard. (9) Second grade pupils prefer reading books which can be easily read, whreeas 5th grade pupils prefer reading books containing difficult sentences. (10) Second grade pupils and the 5th grade pupils both prefer books in accordance with the sex of there heroes. (11) Relationship between second graders' reading preference and the subject matters are rejected at the 10% significance level. A strong relationship, however, appears to exist between 5th graders' reading preference and the subject matter. Fifth grade pupils prefer reading books of which the themes are mystery, exploration and adventure. (12) The theme of books is not proved to relate with the book preference of the 2nd grade pupils, but it is closely related with the book preference of 5th grade pupils. Fifth grade pupils prefer reading books which contain exploratory stories. (13) Second grade pupils are not influenced by the environmental setting of the story, but 5th grade pupils prefer reading stories with the environmental setting in the air. (14) The quantity of illustrations is the element influencing most on .the book preference of the 2nd grade pupils, followed by the space between the lines, which influences least on the book preference of the 5th grade pupils. (15) The physical element influencing most on the book preference of the 2nd grade pupils is the quantity of the illustrations. The physical element which has the most strong influence on the book preference of the 5th graders is illustration styles. (16) The content elements which has much influence on the book preference of the 5th grade pupils are certain kinds of characteristics of heroes. In case of the 2nd grade pupils book preference, striking evidence of such characteristics of heroes is not traced.

  • PDF