• Title/Summary/Keyword: Rehmannia glutinosa L.

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Micropropagation from root segments to improve seedling quality in Chinese foxglove crops

  • Pham, Thanh Loan;Nguyen, Van Huy;Hoang, Thi Le Thu;Ha, Thi Tam Tien;Tran, Trung Kien;Vu, Xuan Duong;Cao, Phi Bang;Nguyen, Quang Trung
    • Journal of Plant Biotechnology
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    • v.47 no.3
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    • pp.235-241
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    • 2020
  • This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.

Effect of Planting Depth on the Quality and Yield in Rehmannia glutinosa LIBOSCHITZ (지황(地黃) 재배시(栽培時) 복토(覆土) 깊이가 품질(品質) 및 수량(收量)에 미치는 영향(影響))

  • Choi, In-Sik;Park, Jae-Seong;Cho, Jin-Tae;Son, Suk-Yong;Chung, In-Myung
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.1
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    • pp.12-15
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    • 1995
  • This experiment was carried out to elucidate the effect of planting depth on the Quality and yield in Rehmannia glutinosa. Planting depths were 3,6,9 and 12cm, and planted on April 18. Emergence rate was decreased in the order of 86% in 3cm, 67% in 6cm, 37% in 9cm and 21% in 12cm. Leaf length was longer about $0.3{\sim}0.8cm$ in planting depth 6-12cm than that of 15.6cm in planting depth 3cm. Yield of Rehmannia glutinosa were lower 5% in planting depth 6cm, 62% in 9cm and 68% in 12cm than that of l,154kg/10a in planting depth 3cm. And optimum planting depth was considered 6cm.

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Effects of Medium , Plant Growth Regulators , and Explant Sources on Plant Regeneration of Rehmannia glutinosa (배지, 생장조절물질 및 치상조직이 지황 체세포조직으로부터 식물체분화에 미치는 영향)

    • Korean Journal of Plant Resources
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    • v.10 no.1
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    • pp.94-99
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    • 1997
  • When the leaf and stem tissues of Rehmannia glutinosa were cultured on MS medium with plant growth regulators, shoots were regenerated on MS medium with TDZ(0.01 to $2.0{\mu}M$), and root initiation was better on MS medium treated with NAA(0.01 to 2.0mg/$\iota$). On $B_5$ medium, shoot regeneration was better on medium with TDZ than those with Quincrac, NAA, and 2.4-D. The addition of Quincrac, NAA, and 2.4-D inhibited shoot regeneration on MS medium, but promoted shoot regeneration on $B_5$ medium. Shoot regeneration and growth was better on MS medium with the combination treatments of 2.4-D 0.01mg/$\iota$ and TDZ 0.01, 0.1 and $2{\mu}M$ compared with other treatments. Also shoot regeneration and growth on B_5 medium showed the similar results to that on MS medium.

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Characterizations of Broad Benn Wilt Virus isolated from Rehmania glutinosa Liboschitz

  • Park, In-Sook;Park, Min-Kyoung;Kim, Jong-Yeop;Lim, Ju-Rak;Lee, Kui-Jae;Lee, Wang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11b
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    • pp.54-54
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    • 2002
  • The nucleotide sequence of a Korea isolate of broad bean wilt favavirus from Rehmannia glutinosa Lib., designated BBWV-RE, was determined. Direct amino acid sequencing of the virus coat proteins suggests that a comparison of several favaviruses in terms of nucleotide sequence and amino acid sequences showed that BBWV-2 isolates display high sequence identity. The small coat protein genes of RNA-2 were also determined for three other Japanese isolates(E, L, and 1-2) and two ATCC isolates(PV132 and PV176) of BBWV. the CP sequence suggested distinct evolution lineages. Serotype 2 favaviruses are more prevalent in Asia, Australia and North America, Wheres serotype 1 is more prevalent in europe.(중략)

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Development of High Throughput Screening Techniques Using Food-borne Library against Anti-asthma Agents (식품소재 라이브러리를 이용한 천식 완화용 물질의 초고속스크리닝 기법 개발)

  • Heo Jin-Chul;Park Ja-Young;Kwon Taeg Kyu;Chung Shin Kyo;Kim Sung-Uk;Lee Sang-Han
    • Food Science and Preservation
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    • v.12 no.3
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    • pp.267-274
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    • 2005
  • Oxidant stress is a well-known pivotal parameter for the degenerative immune diseases including asthma, atopic dermatitis, and rhinitis. In order to screen for anti-asthma agents effectively, we first established the infrastructure of high throughput screening(HTS) for anti-oxidant agents from agricultural products and/or oriental medicine library extracted with water, methanol, dimethyl sulfoxide, ethyl acetate and juice, Using the screening system, we found that Chaenomelis langenariae, Rhus javanica L., Camellia sinensis, Helianthus annuus and Angelica utilis Makino had strong anti-oxidant activity. Moreover, Helianthus annuus, Rehmannia glutinosa Libo and Angelica utilis Makino have protection activities by treatment of an oxidant hydrogen peroxide. Together, these results suggest that screened agents could be potential agents against asthma, although the in vivo studies should be clearly tested.

Quality Characteristics and Antioxidative Activities of Rice Cookies with Rehmannia glutinosa Preparata (숙지황을 첨가한 쌀 쿠키의 품질특성 및 항산화성)

  • Shin, Suk Kyung;Min, A Young;Kim, Hyun Jeong;Lee, Su Jin;Sim, Eun Kyoung;Lee, Kun Jong;Lee, Bo Dam;Kim, Mee Ree
    • Korean journal of food and cookery science
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    • v.31 no.2
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    • pp.136-143
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    • 2015
  • The aim of this study was to evaluate the quality characteristics and anti-oxidative activities in the rice cookies with R. glutinosa Preparata (0%, 4%, 8%, 12%) and bean flour (10%). The moisture of cookies was increased depending on amount of R. glutinosa Preparata (RRP). The pH of cookies with RRP was lower than that of the control cookies. Sugar content and reducing sugar of cookies were increased according to the amount of RRP. L-value and b-value of cookies with 12% RRP were lower than the control, whereas a-value was higher than the control. Spread factor of cookies with RRP was lower than the control, and hardness of cookies was increased depending on amount of RRP added. The antioxidant activity such as DPPH radical and hydroxyl radical scavenging activity was significantly increased in the cookies with RRP, compared with that of the control. Also, total phenol content of cookies was increased according to the amount of RRP. In sensory test, cookies with 8% RRP received the highest score for overall preference. As a result, the optimum amount of RRP concentrate to be added in the cookies was found to be 8%.