• 한국미생물·생명공학회지
• /
• 제42권1호
• /
• pp.73-81
• /
• 2014

본 연구에서는 명아주과 수송나물속 솔장다리(Salsola collina Pall.) 추출물의 항산화 및 항암 활성을 분석하였다. 먼저 솔장다리의 에탄올 추출물의 DPPH radical scavenging activity를 분석한 결과, $IC_{50}$가 $4.82{\mu}g/ml$로 나타나 강한 항산화능을 보유하였음을 확인하였다. 또한 대장암 세포주(HT29), 폐암 세포주(A549), 간암 세포주(HepG2)를 사용하여 솔장다리 추출물의 암세포 사멸효과를 분석한 결과, $IC_{50}$가 각각 43.8, 64.1, $92.5{\mu}g/ml$로 강력한 세포사멸효과를 나타냈으며 특히 HT29에 대한 강한 사멸효과를 보였다. 솔장다리 추출물의 항암 활성 기전 분석을 위해 세포주기를 분석한 결과, 대장암세포인 HT29의 G2/M arrest를 유도하였으며 최고 농도인 $60{\mu}g/ml$까지 S기 세포수가 증가하였다. 세포주기관련 단백질의 발현 분석 결과, 솔장다리 추출물을 처리한 경우, G2기에서 M기로의 전이에 필수적인 단백질인 Cdc25C와 cyclin A의 발현이 감소되었고, 반면 Cdc25C와 Cdc2의 불활성화 형태인 p-Cdc25C, p-Cdc2는 증가하였다. 또한 p21과 Wee1의 발현도 증가되었다. 하지만 p53의 발현량은 변화가 없었다. 이러한 결과는 솔장다리 추출물을 처리한 경우, p53 비의존적으로 p21의 발현이 증가되어 cyclin A/Cdc2 complex의 활성이 조절되고, 이어서 G2/M phase의 check point에 작용하는 Wee1의 발현증가 및 Cdc25C, Cdc2의 인산화에 의한 불활성화를 통하여 G2/M arrest가 유도되는 것을 시사한다. 또한 솔장다리 추출물 처리에 의해 S기 진행을 조절하는 Cdk2의 발현량도 감소하여, cyclin A/Cdk2 complex가 감소되어 S기의 세포수가 증가한 것으로 보인다. 따라서 본 연구 결과를 통해 솔장다리 추출물이 높은 항산화 활성을 지니며 암세포의 세포주기를 조절하여 높은 항암 활성을 보유함을 확인하였다.

• 한국환경과학회지
• /
• 제27권7호
• /
• pp.561-575
• /
• 2018

Agricultural soils around springwaters heavily affected by pesticide run-off and around wells considering the regional characteristics were collected at 24 stations in Jeju Island, and the physicochemical properties and adsorption and leaching characteristics of four nonionic pesticides (diazinon, fenitrothion, alachlor, and metalaxyl) were investigated. The values of the major soil factors affecting the adsorption and leaching of pesticides, namely, soil pH($H_2O$), organic matter content, and cation exchange capacity (CEC), were in the range of 4.64 ~ 8.30, 0.9 ~ 13.1% and 12.7 ~ 31.7 meq/100 g, respectively. The Freundlich constant, $K_F$ value, which gives a measure of the adsorption capacity, decreased in the order of fenitrothion > diazinon > alachlor > metalaxyl, which was identical to their lower water solubility. Among the collected soils, the $K_F$ value was very highly correlated with organic matter content ($r^2=0.800{\sim}0.876$) and CEC ($r^2=0.715{\sim}0.825$) and showed a high correlation with clay content ($r^2=0.473{\sim}0.575$) and soil pH($H_2O$) ($r^2=0.401{\sim}0.452$). The leaching of pesticides in the soil column showed a reverse relationhip with their adsorption in soils, i.e., the pesticides leached more quickly for the soils with lower values of organic matter content and CEC among the soils and for the pesticides with higher water solubility.

• 유통과학연구
• /
• 제13권12호
• /
• pp.95-103
• /
• 2015

Purpose - The Visegrád Group cooperation of the past 14 years and that of V4 for the past 20 years has very important significance in the 21st century that must be maintained. This cooperation is valuable because of the trade routes that connect northern Poland to the Balkans in southern Croatia, which forman important basis for the resuscitation of Central European development. Currently, because of the European manufacturing base and industrial development, an energy supply and stable energy distribution networks have been introduced to secure cooperation and not competition within the Visegrád Group. This paper's research emphasizes the supply chain hub in neighboring countries. Although Central and Eastern European countries are small, they can provide a competitive response to Western Europe if they collaborate with the V4 group and other countries. Research design, data, and methodology - The subjects of this study in the Visegrád Group area are related to the development of Marketing and Distribution Sciences in the integrated European Union. In relation to the existing energy infrastructure, construction companies and financial institutions benefit from large-scale construction projects. Existing or new infrastructure facilities among the V4 must comply with the preconditions of regional energy markets. The network of emerging markets is changing into a European-logistics hub of new markets. This hub is closely associated with the economic development of European self-sustainment given that energy for distribution and consumption is imported from Russia. Therefore, this paper indirectly provides data on the regional distribution of energy as alternative bases in Europe for market expansion to Asia. Results - As a result, it appeared unlikely that V4 failed to implement homogeneity following the standards of Western Europe, as proposed by the EU. Throughout European history, individuals have gathered in Central Europe as an innovation hub. Currently, the region is being established independently for energy industrial development and not for tourism development, and is expected to play a central role in innovation and distribution consumption. Therefore, similar to Western and Northern Europe, V4 only appears to engage in distribution consumption on the basis of the identity that it formed for itself. This area is expected to either create a regional platform or a voice over a single economic policy. Conclusions - To this end, regarding the distribution of consumer groups within and outside the region, the V4 group is expected to be established for various policy areas and as a Eurasian outpost of trade and distribution logistics. In addition, given its purpose of engaging in the distribution of energy cooperation and trade clusters, the Visegrád Group will be in charge of the center axis of the bridge for distribution logistics trading partners from the Western Balkans to Caucasus and Eastern Europe. Thus, the Visegrád Group is entering this region as a platform for market share by enabling all or any investor can gain greater industrial benefits.

• 한국식품영양과학회지
• /
• 제44권3호
• /
• pp.356-362
• /
• 2015

제주산 손바닥 선인장의 엽상경을 열수와 70% 에탄올로 추출한 후 분획하여 총 페놀 및 총 플라보노이드 함량과 항산화 활성 및 항염증 활성을 측정하였다. 총 페놀 함량은 에틸 아세테이트 분획물이 784 mg GAE/g으로 가장 높았고 그 다음으로 부탄올 분획물(452), 헥산 분획물(220) 순이었다. 총 플라보노이드 함량도 에틸아세테이트 분획물이 214 mg GE/g으로 가장 높았고 헥산 분획물(113), 부탄올 분획물(76) 순이었다. 에틸아세테이트 분획물, 부탄올 분획물, 헥산 분획물의 DPPH 라디칼 소거능($IC_{50}$)은 각각 103, 359, $469{\mu}g/mL$였고, ABTS 라디칼 소거능($IC_{50}$)도 각각 105, 379, $605{\mu}g/mL$로 에틸아세테이트 분획물이 가장 높았다. 유해산소 억제 능력(ORAC)은 에틸아세테이트 분획물이 $391{\mu}M$ TE로 가장 높은 활성을 보였고 부탄올 분획물(117), 헥산 분획물(64) 순이었지만, superoxide anion 소거 활성($IC_{50}$, ${\mu}g/mL$)은 에틸아세테이트 분획물(40), 부탄올 분획물(69), 70% 에탄올(98) 순이었다. RAW264.7 세포에 의한 NO 생성 저해능($IC_{50}$, ${\mu}g/mL$)은 헥산 분획물(62), 에틸아세테이트 분획물(104), 부탄올 분획물(465) 순으로 높은 활성을 보였다. 세포독성 대비 NO 생성 저해 활성을 나타내는 SI 지수는 에틸아세테이트 분획물이 4.63으로 가장 높았고, 다음으로는 헥산 분획물(3.37), 부탄올 분획물(2.14), 증류수 추출물(1.66) 순으로, 양성 대조군으로 사용한 quercetin(6.25)보다는 낮았다. 결론적으로 제주산 손바닥 선인장 엽상경의 추출물과 분획물 중에서 에틸아세테이트 분획물이 항산화 활성과 항염 활성이 가장 높았으며, 이는 총 페놀과 총 플라보노이드 함량에 기인하는 것으로 추정되었다.

• 한국재난정보학회 논문집
• /
• 제16권2호
• /
• pp.211-222
• /
• 2020

연구목적: 재난안전 마스터플랜의 가이드라인을 제시하고, 이를 통한 지역안전지수 향상방안을 도출한다. 연구방법: 재난 관련 법률, 안전혁신마스터플랜, 국제안전도시지표와의 정합성 진단을 통해 지자체의 재난안전 관리체계를 수립하고 지역 특성과 분야별 재난관리 대책을 포함하는 재난안전 마스터플랜 가이드라인을 제시한다. 연구결과: 'G'기초지자체를 선정하여 재난안전 마스터플랜 가이드라인을 적용한 세부정책을 수립하고, 이를 통해 지역안전지수 개선방안을 도출한다. 결론: 지역안전지수 향상을 통해 지자체의 재난대응 역량을 강화한다.

• Preventive Nutrition and Food Science
• /
• 제19권2호
• /
• pp.89-97
• /
• 2014

Broccoli (Brassica oleracea var. italia) florets were extracted with 80% methanol and the extract was sequentially fractionated with n-hexane, ethyl acetate, n-butanol, and distilled water. The extract and the fractions were evaluated for total phenolic content, sulforaphane content, antioxidant activity, and anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The total phenolic content and sulforaphane content of the ethyl acetate fraction (EF) were 35.5 mg gallic acid equivalents/g and $620.2{\mu}g/g$, respectively. These values were higher than those of the 80% methanol extract and organic solvent fractions. The oxygen radical absorbance capacity of the EF [$1,588.7{\mu}M$ Trolox equivalents (TE)/mg] was 11-fold higher than that of the distilled water fraction ($143.7{\mu}M\;TE/mg$). The EF inhibited nitric oxide release from LPS-stimulated RAW 264.7 cells in a dose-dependent manner and inhibited $I{\kappa}B-{\alpha}$ degradation and nuclear factor-${\kappa}B$ activation in LPS-stimulated RAW 264.7 cells. In conclusion, the EF of broccoli florets exerted potent antioxidant and anti-inflammatory effects.

• 정보학연구
• /
• 제8권3호
• /
• pp.143-158
• /
• 2005

The purpose of the paper is to analyze situations and its prospect of Single Window System for custom's one-stop service in Japan and to suggest effective application was to improve of in Korea's Customs systems. In response to the growing demand for lower trading costs and shorter and more predictable lead times with the advancement of the international trade supply chain, the Single Window System for import/export and port-related procedures was launched on 23 July 2003 in Japan. The concept of the Single Window System is to establish a comprehensive computer interface system that enables users to complete all import/export and port-related procedures required under different laws and regulations in a single input and single transmission. To realize the concept, the data elements and submission times of the various systems were harmonized, and manual procedures, such as quarantine and immigration, were computerized. Then, all the necessary systems, such as NACCS, Port EDI System, and Crew Landing Permit Support System, were interconnected as a Single Window System. Close cooperation among other governmental organizations and the private sector was a key factor in the successful development and smooth utilization of the System so that it fully met all parties' needs. It is anticipated that operating costs will be greatly reduced and trade facilitation will be enhanced as a result of the simplification of procedures. Korea Customs advocates the Single Window System as a best practice at ASEAN and WCO to contribute to the development of regional and international capacity building.

• Strategy21
• /
• 통권43호
• /
• pp.57-88
• /
• 2018

Security situations are fundamentally and rapidly changing on the Korean Peninsula. Above all, as North Korea(NK) is heightening its nuclear and missile capabilities, Republic of Korea(ROK) is facing an existential threat. At the same time, as China's economic, diplomatic and military power is quickly rising, the balance of power is shifting and strategic competition between the Unite States(US) and China is accelerating in the Asia-Pacific region. Under the pressure of development of these situations, ROK seems to face allegedly the most serious crisis in its national security since the end of the Korean War. In the current grim geopolitical situation, maritime security may become the most difficult security challenge for ROK in the years to come. The purpose of this paper is to compare major changes in maritime security affairs of the ROK during last twenty years from 1998 until now(2018). 1998 was when this journal 『Strategy 21』 was published for the first time by the Korea Institute for Maritime Strategy. Then, this paper tries to identify challenges and risks with which this country has to deal for its survival and prosperity, and to propose some recommendations for the government, the Navy, and the Coast Guard as they are responsible for the maritime security of the country. The recommendations of this paper are as follows: strengthen ROK-US alliance and expand security cooperation with regional powers in support of the maintenance of the current security order in the region; building-up of maritime security capacity in preparation for crisis on the maritime domain with the navy targeting to acquire 'a non-nuclear, balance-of-terror capability, to improve interoperability with the Coast Guard based on 'a national fleet,' and to actively pursue innovation in naval science and technology. Finally, this paper proposes that naval capability the country needs in another twenty years depends on how effectively and rigorously the navy put its utmost efforts towards building 'a strongest navy' today.

• 한국농공학회논문집
• /
• 제63권3호
• /
• pp.1-12
• /
• 2021

Optimal water management is to efficiently and equally supply an appropriate amount of water by using irrigation facilities. Therefore, it is necessary to evaluate water supply capacity through distribution simulation between the designed distribution rate and re-distributed rate according to the changed farming conditions. In this study, we recalculated the agricultural water supply amount of Geumcheon main canal, which beneficiary area was reduced due to the development of Gwangju-Jeonnam innovation city, and we constructed a canal network using the SWMM model to simulate the change in supply rate of each main canal according to the re-distributed rate. Even though the supply amount of the Geumcheon main canal was reduced from 1.20 m³/s to 0.90 m³/s, it showed a similar supply rate to the current, and the reduced quantity could be supplied to the rest of the main canal. As a result, the arrival time at the ends of all main canal, except for the Geumcheon main canal, decreased from 1 to 3 hours, and the supply rate increased from 4 to 17.0% at the main canal located at the end of the beneficiary area of Naju reservoir.

• 대한지역사회영양학회지
• /
• 제28권2호
• /
• pp.95-103
• /
• 2023

Objectives: The budget gap in the health sector of local governments affects the supply of health services, which can cause the health gap. This study classified local governments according to their financial characteristics, such as local financial independence and health budget level. It analyzed the health behaviors and disease prevalence of local residents to examine the effect of local government financial investment on the health of local residents. Methods: To classify types according to the financial characteristics of local governments, financial independence and the health budget data for 17 local governments were collected from the local fiscal yearbook of the Ministry of Public Administration and Security. The prevalence of chronic diseases and healthy behavior was compared using the 16,333 data of adults between the ages of 30 and 65 years among the original data of the National Health and Nutrition Examination Survey (2016-2020). Results: Cluster analysis was used to classify local governments into five clusters according to the health financial capacity type. A comparison of the prevalence of local residents by cluster revealed a similar prevalence of hypertension, diabetes, and hypercholesterolemia. On the other hand, the obesity rate (P < 0.01), high-risk drinking rate (P < 0.01), aerobic physical activity rate (P < 0.001), and healthy eating practice rate (P < 0.001) were significantly different. In addition, an analysis of the odds ratio based on the Seoul area revealed a higher risk of health behavior of non-Seoul residents. Conclusions: It is necessary to review the universal health promotion project budget considering the degree of regional financial vulnerability from the viewpoint of health equity to narrow the health gap among regions.