• 제목/요약/키워드: Regeneration rate

검색결과 700건 처리시간 0.029초

Stand Structure and Regeneration Pattern of Kalopanax septemlobus at the Natural Deciduous Broad-leaved Forest in Mt. Jeombong, Korea

  • Kang, Ho-Sang;Lee, Don-Koo
    • Journal of Ecology and Environment
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    • 제29권1호
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    • pp.17-22
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    • 2006
  • Since the demands not only for value-added timber but the environmental functions of forests had been increased, native tree species has been, and is rapidly being replaced by foreign tree species in many parts of the world. However, the studies on population structure and regeneration characteristics of native tree species were not conducted enough. Regeneration of Kalopanax septemlobus growing among other hardwoods in natural forests is very difficult because of its low seed viability and germination rate. The study examined the distribution of mature trees of K. septemlobus and their regeneration pattern at the 1.12 ha study plot in natural deciduous broad-leaved forest of Mt. Jeombong. The density and mean DBH of K. septemlobus was 97 trees per ha and 32 cm, respectively. The spatial distribution of K. septemlobus showed a random pattern (aggregation index is 0.935) in the 1.12 ha study plot. The age of 90 trees among 99 sample trees of K. septemlobus ranged from 90 to 110 years and represented a single cohort, thus suggesting that K. septemlobus in advance regeneration has regenerated as a result of disturbances such as canopy opening.

Influence of formulated organic Plant tissue culture medium in the shoot regeneration study of Brassica juncea (l.) - Indian mustard

  • Kashyap, Suman;Tharannum, Seema;R, Taarini
    • Journal of Plant Biotechnology
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    • 제46권2호
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    • pp.114-118
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    • 2019
  • Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.

태양열을 이용한 개방형 제습.건조시스템의 저장탱크 유동특성에 관한 연구(제1보 재생기용 저장탱크의 유동특성에 관한 실험) (PStudy on Characteristics of Dryness Storage Tank, Component of Open-type Dehumidifying and Drying System using Solar Energy(I Experiment on Flowing Characteristics of Storage Tank for Regenerator))

  • 김종률;최광환;금종수;김종렬
    • 태양에너지
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    • 제20권2호
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    • pp.1-8
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    • 2000
  • The present study has been conducted to offer geometrically optimal structure of a storage tank for a regenerator, one of the components of an open-type dehumidifying and drying system using solar energy to develop an alternatives for time-limited energy such as a fossil fuel, and to decrease an environmental pollutant. Two kinds of model classified by the mixing process were suggested to estimate the amount of regeneration rate. One was the stratified-type, "case 1", a method to place an entrance in the upper part of the tank and an outlet in the lower part. The other was the mixed-type, "case 2", a method to place vice versa. Solution temperature and concentration were used to evaluate the regeneration rate. As a result, the regeneration rate was better in "case 2".

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태양열을 이용한 개방형 제습.건조시스템의 저장탱크 유동특성에 관한 연구(제2보 배플 설치의 영향에 관한 실험) (Study on Characteristics of Dryness Storage Tank, Component of open-type Dehumidifying and Drying System using Solar Energy (2nd Paper: Experiment on Influence of Baffle Plates Installed in the Tank))

  • 김종률;최광환;금종수;김종렬
    • 태양에너지
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    • 제20권4호
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    • pp.25-30
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    • 2000
  • The present study has been conducted to offer an geometrically optimal structure of a storage tank for a regenerator. In this paper, three kinds of baffle plates supposed to increase the regeneration rate and classified as "Type A($L^*$=0.25, 80(L)$\times$300(W)$\times$50 (H) [mm])", "Type B($L^*$=0.5, 150(L)$\times$300(W)$\times$50 (H) [mm])", and "Type C($L^*$=0.75, 230(L)$\times$300(W)$\times$50(H) [mm])" were suggested and tested. They were placed between the vertical C-C layers in the tank. Solution temperature and concentration were used to evaluate the regeneration rate. As a result, the regeneration rate was better in "Type C".

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Multiple Shoots Regeneration and In vitro Bulblet Formation from Garlic Callus

  • Kim Soon-Seob;Guo De-Ping;Jung Do-Cheol;Kwon Soon-Tae
    • Journal of Plant Biotechnology
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    • 제5권2호
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    • pp.95-99
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    • 2003
  • The leaf segments of garlic (Allium sativum L.) were cultured in vitro and determined optimal concentration of plant growth regulators and sugars for callus induction, multiple shoots regeneration and in vitro bulblet formation. Highest yield of callus was observed in the leaf segment culture on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-D, 30 g/L sucrose and 8 g/L agar. Regeneration rate of multiple shoots from callus was high in the MS medium supplemented with kinetin 3.0 + NAA 3.0 mg/L or SA 1.0 + NAA 3.0 mg/L, containing 30 g/L sucrose. High rate of bulblet formation was observed as the concentration of jasmonic acid increased from 0.5 to 2.0 mg/L in medium, whereas addition of gibberellic acid significantly suppressed bulblet formation. The rate of in vitro bulbing was as high as $96\%$ in MS medium supplemented with 2.0 mg/L jasmonic acid and 120 g/l sucrose after two month culture at $25{\pm}1^{\circ}C$ under 16 hours day light.

수종의 흡수성 차단막의 생체 분해도와 조직학적 반응 (Resorbability and histological reaction of bioabsorbable membranes)

  • 석헌주;권석훈;김창성;최성호;전동원;김종관
    • Journal of Periodontal and Implant Science
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    • 제32권4호
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    • pp.781-800
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    • 2002
  • The major goals of periodontal therapy are the functional regeneration of periodontal supporting structures already destructed by periodontal disease. There have been many efforts to develop materials and therapeutic methods to promote periodontal wound healing. With the development of non-resorbable membrane, GTR has proved to be the representive technique of periodontal regeneration. However, due to various clinical problems of non-resorbable membrane, resorbable membrane was developed and it showed to be clinically effective. The newly developed Para-Dioxanone membrane has a characteristic of non-woven fabric structures which is different from the generally used membranes with structure of mesh form. In addition, Chitosan membrane has been developed to apply its adventage maximally in GTR. Although a number of different types of membranes had been clinically used, researches on absorption rate of membranes were inadequate and limited to subjective opinions. However, since long term period of resorption and space maintenance are required in implant or ridge augmentation, accurate verification of resorption rate is clinically important. In this study, we had implanted Resolut(R), Biomesh(R), Para-Dioxanone membrane and Chitosan membrane (Size : 4mm ${\times}$ 4mm) on dorsal side of Sprague Dawley rat, and sacrified them after 4 weeks, 8 weeks, 12 weeks respectively. Histologic observation was carried out, and the following results were obtained by calculating the objective resorption rate. 1. In case of Resolut(R), external resorption took place initially, followed by internal resorption. Surface area are 5.76${\pm}$2.37$mm^2$, 4.90${\pm}$l.06$mm^2$, 4.90${\pm}$0.98$mm^2$ at 4 weeks, 8 weeks, 12 weeks respectively, and invasion rate of connective tissue to membrane are 31.6${\pm}$4.5%, 52.8${\pm}$9.4%, 56.4${\pm}$5.1% respectively. 2. Biomesh(R) showed a pattern of folding, relatively slow resorption rate with small size of membrane. Surface area are 3.62${\pm}$0.82$mm^2$, 3.63${\pm}$0.76$mm^2$, 4.07${\pm}$1.14$mm^2$ at 4 weeks, 8 weeks, 12 weeks respectively, and invasion rate of connective tissue to membrane are 26.1${\pm}$5.8%, 30.9${\pm}$3.4%, 29.2${\pm}$3.6%, respectively. 3. Para-Dioxanone membrane was surrounded by fibrous conncetive tissue externally, and resorption took place internally and externally. Surface area are 5.96${\pm}$1.05$mm^2$, 4.77${\pm}$10.76$mm^2$, 3.86${\pm}$0.84$mm^2$ at 4 weeks, 8 weeks, 12 weeks respectively, and invasion rate of connective tissue to membrane are 30.7${\pm}$5.1%, 53.3${\pm}$4.4%, 69.5${\pm}$3.1%, respectively. 4. Each fiber of Chitosan membrane was surrounded by connective tissue and showed external resorption pattern. It showed little invasion of inflammatory cells and excellent biocompatability. The resorption rate was relatively slow. Surface area are 6.01${\pm}$2.01$mm^2$, 5.49${\pm}$1.3$mm^2$, 5.06${\pm}$1.38$mm^2$ at 4 weeks, 8 weeks, 12 weeks respectively, and invasion rate of connective tissue to membrane are 31.3${\pm}$3.6%, 38.4${\pm}$3.80%, 39.7${\pm}$5.6%, respectively. Consequently, Para-Dioxanone membrane and Chitosan membrane are found to be clinically effective for their excellent tissue reaction and biocompatibility. Futhermore, the advantage of bone regenerating ability as well as the relatively long resorption period of Chitosan membrane, it might be widely used in implant or ridge augmentation.

스프레이 국화 형질전환을 위한 국내 육성 품종 선발 (The Selection of Domestically Bred Cultivars for Spray-type Chrysanthemum Transformation)

  • 서은정;한봉희;이연희;이성곤;홍준기;김경환
    • 원예과학기술지
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    • 제33권6호
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    • pp.947-954
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    • 2015
  • 질전환에 적합한 국화 스프레이 타입 품종을 선발하기 위해서 국내 연구기관에서 육성된 39개 품종 및 일본품종인 스탠다드 타입 신마를 모아 재분화율과 아그로박테리움 감염도를 조사하였다. 잎과 마디 절편체로부터의 신초 재분화를 위하여 IAA $0.5mg{\cdot}L^{-1}$와 BAP $1.0mg{\cdot}L^{-1}$가 첨가된 MS 배지를 사용하였다. 잎 절편체로부터의 신초 재분화는 BRM 품종이 가장 높았고 VS, WW, YTM 품종순으로 분화되었다. 이전에 형질전환재료로 보고된 바 있는 신마는 PK, SPP 품종과 유사한 수준을 보였다. 마디 절편체로부터의 신초 재분화는 PA 품종에서 가장 높았으며 WW 품종이 다음으로 확인되었다. 40개의 국화 품종을 대상으로 아그로박테리움에 대한 감염도를 조사한 결과, 잎 절편체에서는 WPP, YNW, VS, PP, WW, FA, PA, YMN 품종이 가장 높았고 마디 절편체에서는 WPP, PA, PK, YNW 품종이 가장 높았다. 이상의 결과를 종합해 볼 때 국화의 잎 절편체를 이용한 형질전환은 VS와 WW 품종이 가장 적합한 반면에 마디형질전환에는 PA 품종이 가장 적합한 것으로 확인되었다.

CO2 농도와 온도상승이 칠면초, 퉁퉁마디, 해홍나물 그리고 나문재의 생육 반응 및 개체군 재생율에 미치는 영향 (Effects of Elevated CO2 and Elevated Temperature on the Growth Response and Regeneration Rate of Population of Halophytes - Suaeda japonica, Salicornia europaea, Suaeda maritima and Suaeda glauca -)

  • 김지은;김의주;이응필;박재훈;이승연;박지원;이정민;최승세;유영한
    • 생태와환경
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    • 제53권3호
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    • pp.295-303
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    • 2020
  • We investigated the growth response and population regeneration of four halophyte species: Suaeda japonica, Salicornia europaea, Suaeda maritima and Suaeda glauca, when climate change proceeds caused by increased CO2 concentration and temperature. The plants collected from habitat in 2018 were transplanted into Wagner pots, and cultivated for two years in greenhouse divided into a control (ambient condition) and a treatment (elevated CO2+elevated temperature). The shoot length of halophytes was measured in July of each year, and the population regeneration rate was measured in October 2019. The shoot lengths of S. japonica and S. glauca had no difference between control and treatment for two years. Those of S. europaea were longer in control than treatment for two years. Those of S. maritima had no difference between control and treatment in 2018 but were longer in control than treatment in 2019. In control, the shoot lengths of S. japonica, S. europaea and S. glauca had no difference between years while those of S. maritima were longer in 2018 than in 2019. In treatment, those of S. japonica, S. europaea and S. maritima were shorter in 2019 than 2018 but S. glauca had no difference between years. The regeneration rates of S. japonica, S. europaea and S. glauca were lower treatment than control, and there was no difference in the regeneration rate of S. maritima. In conclusion, if climate change progresses caused by the increase of CO2 concentration and temperature, the shoot lengths of S. japonica, S. europaea and S. maritima will be shortened, and the regeneration rate of population will be increased only in the S. maritima.

디지털 공제술을 이용한 $Br{\aa}nemark$ 임플란트 주위 골조직 분석 (Evaluation of peri-implant bone density changes in $Br{\aa}nemark$ implants by computer assisted densitometric image analysis (CADIA))

  • 소성수;노현수;김창성;최성호;채중규;김종관;조규성
    • Journal of Periodontal and Implant Science
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    • 제37권1호
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    • pp.137-150
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    • 2007
  • CADIA(Computer-assisted densitometric image analysis) method is used to analyze bone density changes around the implants. The usefullness and reproducibility of the method was assessed. We tried to find out if there is any possibility to quantitiate and qualitify peri-implant bone density change as time passes. And we concluded that this newly developed linear analysis is efficient for analyzing peri-implant bone density change non-Invasively. In this study, 2152 machined $Br{\aa}nemark$ fixtures installed from 1994 to 2002 in the department of Periodontics, Dental hospital of College of Dentistry, Yonsei University were included. Of these fixtures 22 radiographically analyzable failed fixtures were used as experimental group, and 22 successful implants placed in the same patient were used as control group. 1. 57 out of 1635 machined $Br{\aa}nemark$ standard and Mk II implants system failed, the survival rate was 96.5%. And 11 out of 517 machined $Br{\aa}nemark$ Mk III and Mk IV implants system failed, the survival rate was 97.9%. Total survival rate was 96.8%. 2. 22 failed implants were used for the analysis, 10 of which failed before prosthetic treatment due to infection and overheating. 12 failed due to overload after prosthetic treatment, 63.6% of which failed during the early phase of functional loading, i, e. before 1 year of loading. 3. Bone density change values around coronal region of the failed implants were $-6.54{\pm}6.35$, middle region were $-3.53{\pm}5.78$, apical region were $-0.75{\pm}10.33$, resulting in average of $-3.71{\pm}8.03$. 4. Bone density change values around coronal region of the successful implants were $4.25{\pm}4.66$, middle region were $6.33{\pm}5.02$, apical region were $9.89{\pm}4.67$, resulting in average of $6.27{\pm}5.29$. 5. There was a statistically significant difference between two groups (p<0.01). In conclusion, the linear analysis method using computer-assisted densitometric image analysis could be a useful method for the analysis of implants, and could be used for future implant researchs.

Investigation of bone formation using calcium phosphate glass cement in beagle dogs

  • Lee, Seung-Bum;Jung, Ui-Won;Choi, Youn-A;Jamiyandorj, Otgonbold;Kim, Chang-Sung;Lee, Yong-Keun;Chai, Jung-Kiu;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • 제40권3호
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    • pp.125-131
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    • 2010
  • Purpose: Among available biomaterials, bioceramics have drawn special interest due to their bioactivity and the possibility of tailoring their composition. The degradation rate and formulation of bioceramics can be altered to mimic the compositions of the mineral phase of bone. The aim of this study was to investigate the bone formation effect of amorphous calcium phosphate glass cement (CPGC) synthesized by a melting and quenching process. Methods: In five male beagle dogs, $4{\times}4$ mm 1-wall intrabony defects were created bilaterally at the mesial or distal aspect of the mandibular second and fourth premolars. Each of the four defects was divided according to graft materials: CPGC with collagen membrane (CM), biphasic calcium phosphate (BCP) with CM, CM alone, or a surgical flap operation only. The dogs were sacrificed 8 weeks post-surgery, and block sections of the defects were collected for histologic and histometric analysis. Results: There were significant differences in bone formation and cementum regeneration between the experimental and control groups. In particular, the CPGC and BCP groups showed greater bone formation than the CM and control groups. Conclusions: In conclusion, CPGC was replaced rapidly with an abundant volume of new bone; CPGC also contributed slightly to regeneration of the periodontal apparatus.