Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지
DOI QR코드

DOI QR Code

The Selection of Domestically Bred Cultivars for Spray-type Chrysanthemum Transformation

스프레이 국화 형질전환을 위한 국내 육성 품종 선발

  • Suh, Eun-Jung (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Bong Hee (Department of Seed Service, The Foundation of Agri. Tech. Commercialization and Transfer) ;
  • Lee, Yeon-Hee (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Seong-Kon (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Joon Ki (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Kyung Hwan (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration)
  • 서은정 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 한봉희 (농업기술실용화재단 종자사업팀) ;
  • 이연희 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 이성곤 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 홍준기 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 김경환 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과)
  • Received : 2015.02.13
  • Accepted : 2015.08.14
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

To select suitable spray chrysanthemum cultivars for Agrobacterium-mediated transformation, thirty-nine (39) spray cultivars bred in the National Institutes of Korea and a standard cultivar Jinba from Japan were collected and tested for regeneration rate and Agrobacterium infection assays. MS medium with $0.5mg{\cdot}L^{-1}$ IAA and $1.0mg{\cdot}L^{-1}$ BAP was used for shoot regeneration from leaf disks and internodes. The shoot regeneration rate in leaf disks was the highest in cultivar BRM, followed by cultivars VS, WW and YTM. The cultivar JB (Jinba) used as a transformation material in previous reports ranked similarly to cultivars PK and SPP. In shoot regeneration from internodes, the shoot regeneration rate was the highest for cultivar PA, followed by cultivar WW. The infection rate of leaves and internodes of 40 chrysanthemum cultivars with agrobacterium was investigated. Cultivars WPP, YNW, VS, PP, WW, FA, PA and YMN showed the highest infection levels in leaves, whereas cultivars WPP, PA, PK and YNW had the highest infection levels in internodes. Considering all of these results, cultivars VS and WW were the most appropriate for gene transformation of chrysanthemum using leaves, while cultivar PA was for internodes.

질전환에 적합한 국화 스프레이 타입 품종을 선발하기 위해서 국내 연구기관에서 육성된 39개 품종 및 일본품종인 스탠다드 타입 신마를 모아 재분화율과 아그로박테리움 감염도를 조사하였다. 잎과 마디 절편체로부터의 신초 재분화를 위하여 IAA $0.5mg{\cdot}L^{-1}$와 BAP $1.0mg{\cdot}L^{-1}$가 첨가된 MS 배지를 사용하였다. 잎 절편체로부터의 신초 재분화는 BRM 품종이 가장 높았고 VS, WW, YTM 품종순으로 분화되었다. 이전에 형질전환재료로 보고된 바 있는 신마는 PK, SPP 품종과 유사한 수준을 보였다. 마디 절편체로부터의 신초 재분화는 PA 품종에서 가장 높았으며 WW 품종이 다음으로 확인되었다. 40개의 국화 품종을 대상으로 아그로박테리움에 대한 감염도를 조사한 결과, 잎 절편체에서는 WPP, YNW, VS, PP, WW, FA, PA, YMN 품종이 가장 높았고 마디 절편체에서는 WPP, PA, PK, YNW 품종이 가장 높았다. 이상의 결과를 종합해 볼 때 국화의 잎 절편체를 이용한 형질전환은 VS와 WW 품종이 가장 적합한 반면에 마디형질전환에는 PA 품종이 가장 적합한 것으로 확인되었다.

Keywords

References

  1. Aida, R., Y. Tabei, M. Hirai, and M. Shibata. 1992. Agrobacteriummediated transformation of chrysan-themum. Breed. Sci. 42:270-271.
  2. An, J., A. Song, Z. Guan, J. Jiang, F. Chen, W. Lou, W. Fang, Z. Liu, and S. Chen. 2014. The over-expression of Chrysanthemum crassum CcSOS1 improves the salinity tolerance of chrysanthemum. Mol. Biol. Rep. 41:4155-4162. https://doi.org/10.1007/s11033-014-3287-2
  3. Chen, L. 2005. Research and analysis of the international market of chrysanthemum. Greenhouse Hortic. 8:20-22.
  4. De Jong, J., W. Rademaker, and K. Ohishi. 1995. Agrobacteriummediated transformation of chrysan-themum. Plant Tissue Cult. Biotechnol. 1:38-42.
  5. Duncan, D.B. 1955. Multiple range and multiple F test. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  6. Fukai, S., J. De Jong, and W. Rademaker. 1995. Efficient genetic transformation of chrysanthemum (Dendranthema grandiforum (Ramat.) Kitamura) using stem segment. Breed. Sci. 45:179-184.
  7. Han, B.H., E.J. Suh, S.Y. Lee, H.K. Shin, and Y.P. Lim. 2007. Selection of non-branching lines induced by introducing Ls-like cDNA into chrysanthemum (Dendranthema x grandiflorum (Ramat.) Kitamura) 'Shuho-no-chikara'. Sci. Hortic. 115:70-75. https://doi.org/10.1016/j.scienta.2007.07.012
  8. Han, B.H., S.Y. Lee, and B.M. Park. 2009. Comparison of chrysanthemum cultivars based on direct shoot regeneration rates in tissue culture. J. Plant Biotechnol. 36:275-280. https://doi.org/10.5010/JPB.2009.36.3.275
  9. Hwang, J.C., Y.D. Chin, Y.M. Chung, S.K. Kim, C.W. Ro, and B.R. Jeong. 2013. A new spray chrysanthemum cultivar, 'Blue Hope' with anemone type and white petals for cut flower. Korean J. Hortic. Sci. Technol. 31:123-127. https://doi.org/10.7235/hort.2013.12183
  10. Jefferson, R.A. 1987. Plant reporter genes: The gus gene fusion system. Genetic Engineering 10:247-263.
  11. Kim, Y.H., H.M. Park, J.Y. Jung, T.M. Kwon, S.J. Jeung, Y.B. Yi, G.T. Kim, and J.S. Nam. 2010. Development of Agrobacteriummediated transformation method for domestically bred chrysanthemum cultivar 'Moulinrouge' and genetic change of leaf morphology using AtSICKLE gene. Korean J. Hortic. Sci. Technol. 28:449-455.
  12. Kosugi, S., Y. Ohashi, K. Nakajima, and A. Yuji. 1990. An improved assay for $\beta$-glucuronidase in trans-formed cells: Methanol almost completely suppresses a putative endogenous $\beta$-glucuronidase. Plant Sci. 70:133-140. https://doi.org/10.1016/0168-9452(90)90042-M
  13. Ledger, S.E., S.C. Deroles, and N.K. Given. 1991. Regeneration and Agrobacterium-mediated transformation of chrysanthemum. Plant Cell Rep. 10:195-199.
  14. Lee, S.Y., B.H. Han, E.J. Hur, H.K. Shin, S.T. Kim, E.K. Lee, W.H. Kim, O.H. Kwon, and I.H. Lee. 2012. FT-transgenic spray-type chrysanthemum (Dendranthema grandiflorum Kitamura) showing early flowering. J. Plant Biotechnol. 39:140-145. https://doi.org/10.5010/JPB.2012.39.3.140
  15. Lee, S.Y., J.H. Kim, K.S. Cheon, E.K. Lee, W.H. Kim, O.H. Kwon, and H.J. Lee. 2013. Phenotypic and molecular characteristics of second clone ($T_0V_2$) plants of the LeLs-antisense genetransgenic chrysanthemum line exhibiting non-branching. J. Plant Biotechnol. 40:192-197. https://doi.org/10.5010/JPB.2013.40.4.192
  16. Li, Y.F., W.M. Fang, F.D. Chen, S.M. Chen, and C.L. Shi. 2009. Effect of different planting date on phenophase and quality of spray cut chrysanthemum produced in summer, J. Yangzhou University, Agricultural and Life Sciences ed. 30:80-83.
  17. Miller, M. 1975. Leaf, stem, crown, and root galls induced in chrysanthemum by Agrobacterium tumefaciens. Phytopathology 65:805-811. https://doi.org/10.1094/Phyto-65-805
  18. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  19. Shinoyama, H. and A. Mochizuki. 2006. Insect resistant transgenicchrysanthemum [Dendranthema $\times$ grandiflorum (Ramat.) Kitamura]. Acta Hortic. 714:177-184.
  20. Shinoyama, H., R. Aida, R. Ichikawa, H. Ichikawa, Y. Nomura, and Y. Mochizuki. 2012. Genetic engineering of chrysanthemum (Chrysanthemum morifolium): Current progress and perspectives. Plant Biotechnol. 29:323-337. https://doi.org/10.5511/plantbiotechnology.12.0521a
  21. Song, A., J. An, Z. Guan, J. Jiang, F. Chen, W. Lou W. Fang, Z. Liu, and C. Sumei. 2014. The constitutive expression of a two transgene construct enhances the abiotic stress tolerance of chrysanthemum. Plant Physiol. Biochem. 80:114-120. https://doi.org/10.1016/j.plaphy.2014.03.030
  22. Takatsu, Y., H. Tomotsune, M. Kasumi, and F. Sakuma. 1998. Differences in adventitious shoot regeneration capacity among Japanese chrysanthemum (Dendranthema grandiflora (Ramat.) Kitamura) cultivars and the improved protocol for Agrobacteriummediated genetic transformation. J. Jpn. Soc. Hortic. Sci. 67:958-964. https://doi.org/10.2503/jjshs.67.958
  23. Teixeira da Silva, J.A. 2005. Effective and comprehensive chrysanthemum (Dendranthema $\times$ grandiflora) regeneration and transformation protocols. Biotechnology 4:94-107. https://doi.org/10.3923/biotech.2005.94.107
  24. Teixeira da Silva, J. A., H. Shinoyama, R. Aida, Y. Matsushita, S.K. Raj, and F. Chen. 2013. Chrysanthemum biotechnology: Quo vadis? Crit. Rev. Plant. Sci. 32:21-52. https://doi.org/10.1080/07352689.2012.696461
  25. Teixeira da Silva, J.A. and S. Fukai. 2004. Effect of aminoglycoside antibiotics on in vitro morphogenesis from cultured cells of chrysanthemum and tobacco. J. Plant Biotechnol. 6:25-37.
  26. Urban, L.A., J.M. Sherman, J.W. Moyer, and M.E. Daub. 1994. High frequency shoot regeneration and Agrobacterium-mediated transformation of chrysanthemum (Dendranthema grandiflora). Plant Sci. 98:69-79. https://doi.org/10.1016/0168-9452(94)90149-X

Cited by

  1. 주요 스프레이 국화 품종의 형태적 특성과 변이계수, 유전율 및 유전자 전이율 vol.34, pp.2, 2015, https://doi.org/10.12972/kjhst.20160028