• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권6호
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• pp.531-548
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• 2006

The purpose of this study was to investigate the biomechanical, histologic findings of distracted regenerate and TMJ response in modified distraction osteogenesis (DO) technique combined with compression force as biomechanical stimulation method which has been suggested in 2002, and developed thereafter by authors. This study was performed with two experiments. First experiment was designed to explore the optimal ratio of compression force versus distraction force for the new DO technique. Second experiment was planned to evaluate the reaction of TMJ tissue, especially condyle, disc after application of the DO technique with compression force. Total 52 New Zealand adult male-rabbits with 3.0kg body weight were used for the study. For the first study, 30 adult male-rabbits underwent osteotomy at one side of mandibular body and a external distraction device was applied on each rabbit with same manner. In the control group of 10 rabbits, final 8 mm of distraction with 1 mm rate per day was done with conventional DO technique after 5 latency days. For the experimental group of 20 rabbits, a compression force with 1 mm rate per day was added to the distracted mandible on 3-latency day after over-distraction (over-lengthening). As the amount of the rate of compression versus distraction, experimental subgroup I (10 rabbits) was set up as 2 mm compression versus 10 mm distraction (1/5) and experimental subgroup II (10 rabbits) was set up as 3 mm compression versus 11 mm distraction (about 1/3). All 30 rabbits were set up to obtain final 8 mm distraction and sacrificed on postoperative 55 day to analysis on biomechanical, and histologic findings of the bone regenerates. For second study, 22 adult male-rabbits were used to evaluate TMJ response after the DO method application with compression force. In the control group, 10 rabbits was used to be performed with conventional DO method, on the other hand, in a experimental group of 10 rabbits, 10 mm distraction with 2 mm compression (1/5 ratio) was done. The remaining 2 rabbits served as the normal control group. Histomorphologic examinations on both condyle, histological studies on condyle, disc were done at 1, 2, 3, 4, 7 weeks after distraction force application. The results were as follows: 1. On the bone density findings, the experimental group II (force ratio - 1/3) showed higher bone density than the other experimental group (force ratio - 1/5) and control group (control group - $0,2906\;g/cm^2$, experimental group I - $0.2961\;g/cm^2$, experimental group II - $0.3328\;g/cm^2$). 2. In the histologic findings, more rapid bone maturation like as wide lamellar bone site, more trabeculae formation was observed in two experimental groups compared to the conventional DO control group. 3. In morphologic findings of condyle, there were no differences of size and architecture in the condyle in the control and experimental groups. 4. In histologic findings of condyles, there were thicker fiberous and proliferative layers in experimental group than those of control group until 2 weeks after distraction with compression force. But, no differences were seen between two groups on 3, 4, 7 weeks after compression. 5. In histologic findings of disc, more collagen contents in extracellular matrix, more regular fiber bundles, and less elastin fibers were seen in experimental group than control group until 2 weeks after distraction with compression. But, no differences were seen between two groups on 3, 4, 7 weeks after distraction with compression. From this study, we could identify that the new distraction osteogenesis technique with compression stimulation might improve the quality of bone regeneration. The no remarkable differences on TMJ response between control and experimental groups were seen and TMJ tissues were recovered similarly to normal TMJ condition after 3 weeks.

• Korean Chemical Engineering Research
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• 제48권4호
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• pp.499-505
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• 2010

In this study, hydrodynamics and reaction characteristic of K-based solid sorbents for $CO_2$ capture were investigated using a continuous system composed of two bubbling fluidized-bed reactors(1.2 m tall bed with 0.11 m i.d.). Potassium-based dry sorbents manufactured by the Korea Electric Power Research Institute were used, which were composed of $K_2CO_3$ of 35% for $CO_2$ absorption and supporters of 65% for mechanical strength. The continuous system consists of two bubbling fluidized-bed reactors, solid injection nozzle, riser, chiller, analyzer and heater for regeneration reaction. The minimum fluidizing velocity of the continuous system was 0.0088 m/s and the solid circulation rate measured was $10.3kg/m^2{\cdot}s$ at 1.05 m/s velocity of the solid injection nozzle. The $CO_2$ concentration of the simulated gas was about 10 vol% in dry basis. Reaction temperature in carbonator and regenerator were maintained about $70^{\circ}C$ and $200^{\circ}C$, respectively. Differential pressures, which were maintained in carbonator and regenerator, were about $415mmH_2O$ and $350mmH_2O$, respectively. In order to find out reaction characteristics of dry sorbents, several experiments were performed according to various experimental conditions such as $H_2O$ content(7.28~19.66%) in feed gas, velocity (0.053~0.103 m/s) of simulated gas, temperature($60{\sim}80^{\circ}C$) of a carbonator, temperature($150{\sim}200^{\circ}C$) of a regenerator and solid circulation rate($7.0{\sim}10.3kg/m^2{\cdot}s$). The respective data of operating variables were saved and analyzed after maintaining one hour in a stable manner. As a result of continuous operation, $CO_2$ removal tended to increase by increasing $H_2O$ content in feed gas, temperature of a regenerator and solid circulation rate and to decrease by increasing temperature of a carbonator and gas velocity in a carbonator.

• International Journal of Oral Biology
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• 제32권1호
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• pp.13-22
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• 2007

The stem cell research is emerging as a cutting edge topic for a new treatment for many chronic diseases. Recently, dental stem cell would be possible for regeneration of tooth itself as well as periodontal tissue. However, the study of the cell characterization is scarce. Therefore, we performed the genetic profiling and the characterization of mouse fetus/neonate derived dental tissue and cell to find the identification during dental development. We separated dental arch from mandibles of 14.5 d fetal mice and neonate 0 d under the stereoscope, and isolated dental cells primarily from the tissues. Then, we examined morphology and the gene expression profiles of the primary cells and dental tissues from fetus/neonate and adult with RT-PCR. Primary dental cells showed heterogeneous but the majority was shown as fibroblast-like morphology. The change of population doubling time levels (PDLs) showed that the primary dental cells have growth potential and could be expanded under our culture conditions without reduction of growth rate. Immunocytochemical and flow cytometric analyses were performed to characterize the primary dental cell populations from both of fetus (E14.5) and neonate. Alpha smooth muscle actin (${\alpha}-SMA$), vimentin, and von Willebrand factor showed strong expression, but desmin positive cells were not detected in the primary dental cells. Most of the markers were not uniformly expressed, but found in subsets of cells, indicating that the primary dental cell population is heterogeneous, and characteristics of the populations were changed during culture period. And mesenchymal stem cell markers were highly expressed. Gene expression profile showed Wnt family and its related signaling molecules, growth factors, transcription factors and tooth specific molecules were expressed both fetal and neonatal tissue. The tooth specific genes (enamelin, amelogenin, and DSPP) only expressed in neonate and adult stage. These expression patterns appeared same as primary fetal and neonatal cells. In this study we isolated primary cells from whole mandible of fetal and neonatal mice. And we investigated the characteristics of the primary cells and the profile of gene expressions, which are involved in epithelial-mesenchymal interactions during tooth development. Taken together, the primary dental cells in early passages or fetal and neonatal mandibles could be useful stem cell resources.

• Clean Technology
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• 제9권4호
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• pp.189-196
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• 2003

ZnO-CuO mixed sorbents for desulfurization in hot gas cleaning process Were prepared and investigation on their characteristics was performed in this study. The rate of sulfidation increased as the amount of copper oxide in the composite sorbent was raised. TPO experiments were carried out to investigate the characteristics of the regeneration of the sorbents with severa1 different ratios of Zno to CuO. Copper sulfate was formed at temperatures above $400^{\circ}C$, while it was decomposed by pyrolysis above $700^{\circ}C$. $SO_2$ slippage due to $CuSO_4$ was observed in the sorbent regenerated at temperatures above $600^{\circ}C$. However, it was not observed when regenerated above $700^{\circ}C$. It was confirmed in the ZnO-CuO mixed sorbent system that CuO suppressed the vaporization of ZnO on the one hand and Zno minimized the $SO_2$ slippage due to CuO on the other hand.

• Polymer(Korea)
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• 제36권6호
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• pp.789-794
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• 2012

Poly(lactic-co-glycolic acid) (PLGA) has been most widely used due to its advantages such as good biodegradability, controllable rate of degradation and metabolizable degradation products. We manufactured composite scaffolds of PLGA scaffold penetrated DBP gel (PLGA/DBP gel) by a simple method, solvent casting/salt leaching prep of PLGA scaffolds and subsequent soaking in DBP gel. Chondrocytes were seeded on the PLGA/DBP gel. The mechanical strength of scaffold, histology (H&E, Safranin-O, Alcian-blue) and immunohistochemistry (collagen type I, collagen type II) were performed to elucidate in vitro and in vivo cartilage-specific extracellular matrices. It was better to keep the characteristic of chondrocytes in the PLGA/DBP gel scaffolds than that PLGA scaffolds. This study suggests that PLGA/DBP gel scaffold may serve as a potential cell delivery vehicle and a structural basis for in vivo tissue engineered cartilage.

• Journal of Soil and Groundwater Environment
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• 제8권3호
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• pp.37-44
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• 2003

Fuel oxygenates, such as Methyl tertiary Butyl Ether (MTBE) is additive in gasoline used to reduce air pollution. Gasoline components and fuel additives can leak: form underground storage tanks. MTBE is far more water soluble than gasoline hydrocarbons like BTEX then it travels at essentially the same velocity as groundwater. MTBE in drinking water causes taste and odor problems. Therefore, the purpose of the this study is to examine the ability of ground tire to sorb MTBE in water. The study consisted of running both batch and column tests to determine the sorption capacity, the required sorption equilibration time, and the flow through utilization efficiency of ground tire. The batch test result indicated that ground tire can attain equilibrium sorption capacities about 0.5 mg of MTBE. The result of column test indicate that ground tire has on the 36% utilization rate. Finally, it is clear that ground tire represented an attractive and relatively inexpensive sorption medium for a MTBE. Authors thought that to determine the economic costs of ground tire utilization, the cost to sorb a given mass of contaminant by ground tire will have to be compared to currently accepted sorption media. The cost comparison will also have to include regeneration and disposal cost.

• The Korean Journal of Quaternary Research
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• 제18권2호통권23호
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• pp.107-107
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• 2004

In this paper we have introduced some results of study on stand growth pattern and stand structure of larch forest which are located in selected forest sites of Khangai and Gobi-Altai mountain ranges of Mongolia. Our investigations showed that growth intensity and stand structure in western Mongolia are very specific from the other forest vegetation zones of Mongolia. Studies on the stand structure and growth trend indicate that tree types of stand structure and different types of growth of Larix sibrica are very common in Western Mongolia. These peculiarities of stand structure and growth of larch stands in Western Monolia could be used for inventory work and an improvement of the forest management in Western Mongolian region. The larch tree is the dominant tree species in Western Mogolia. Forest cover of the region is about 15%, which is two times higher than the country's average. In this region forest area is divided into 4 forest sub-regions: the Central Khangai, Western Khangai, North Eastern Khangai and South Easterun Khangai sub-regions including taiga, pseudo taiga, sub taiga, sub-alpine and forest steppe belts. Silviculture practices and forest research management request to study forest growth trends in local and general conditions, which means to indicate a change of taxonomic characteristics of stand from time to time including diameter, height, basal area, growth stock etc. The forest management practice mostly uses tables of forest growth and yield based on the results of long term research on forest growth. Forest yield tables and other relevant forest standards of Russia are used for the forest inventory and forest management. They are not able to determine forest structure and growth peculiatities of Mongolian forests. Studies on forest resource assessment in Mongolia indicate that after logging operations and forest fires the natural regeneration of desired species such as pine and larch often does not succeed. This situation forces to take a different approach of forest management and silviculture practice depending on the stand structure and growth rate of the forest stands. According to our investigation in last years, forest growth pattern of larch forest depends mostly on stand structure, stand age and growth condition including forest soil, climate and location in different slopes. Due to improve environmental function of forest ecosystem in the region, it is needed to conduct very comprehensive study of high mountain forest ecosystem in selected sub-regions.

• Korean Journal of Medicinal Crop Science
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• 제8권1호
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• pp.41-48
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• 2000

This study was aimed to proliferate Gentiana axillariflora Leveille which is one of the important medicinal and ornamental plants, by establishment of multiple shoot formation and embryogenesis through tissue culture technique. Callus was formed on MS (Murashige and Skoog) medium supplemented with 2,4-D, CPA, but not formed with BAP. The addition of 2,4-D 2 mg/ l into the medium was effective for callus formation and the rate of callus formation was about 90%. Somatic embryos were obtained on MS medium for two months. When callus was cultured on MS medium with combination treatment of 2,4-D 0.5 mg/ l and BAP 0.5 mg/ l, the number of embryo formed was better than that of other single or combination treatments and the total numbers of embryo a were 18.8 (number of total embryo/number of explants incubated = 753/40) at mean. Callus induction from stem and node explants was increased by addition of TDZ 2 mg/ l in the presence of 2,4-D 2 mg/ l, respectively. The best result about the differentiation of shoots was obtained on MS medium added BAP 2 mg/ l from node culture. Multiple shoots from shoot apex were induced on MS medium containing BAP 1 mg/ I and TDZ 1 mg/ l , BAP 2 mg/ l and TDZ 1 mg/ l. The number of multiple shoots per one explant was above seventy plants. It was the most effective regeneration system for rapid multiplication of Gentiana axillariflora Leveille.

• Journal of Plant Biotechnology
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• 제33권1호
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• pp.49-56
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• 2006

Gerber (Gerbera hybrida) is a valuable ornamental species grown as a potted plant and cut flowers. However, genetic variability within the gerbera genus is very limited. So it is absolutely needed to introduce and widen genetic resources into gerbera lines by genetic transformation. For the purpose, 18 Korean gerbera lines were screened to establish Agrobacterium-mediated genetic transformation procedure. In an experiment to select Korean gerbera lines which are amenable to Agrobacterium-inoculation, 12 lines turned out to be positive in Agrobacterium-inoculation. More callus were produced from BA 2ppm, Zeatin 2ppm, IAA 0.2ppm in pre-culture and regeneration medium (2X media) but there was no difference in the frequency of GUS expression rate. In another experiment to find out optimal condition for highly efficient Agrobacterium-inoculation, petiole and leaf explants have been treated with four different pre-culture periods, two different co-culture periods and two different Agrobacterium strains. As a result, high GUS expression has been showed from petiole and leaf explants treated no pre-culture period with LBA4404 Agrobacterium tumerfaciens, 5 day co-culture period and dipping treatment.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권6호
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• pp.579-585
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• 2006

Introduction: The fibular free flap is now considered as the first choice for long mandibular discontinuity defect. In spite of its good bone quality for implant installation, its diameter is too narrow to rehabilitate the masticatory function with implant installation. In this report, distraction osteogenesis was used for the augmentation of bone to install the dental implant in the mandible which was reconstructed with a vascularized fibular free flap. Patients and Methods: Three patients undertook the vertical augmentation of grafted fibular bone and dental implants were installed. On the day 8 post-surgery, the activation of the distractor was started at the rate of 1 mm twice a day. The total amount of distraction was 15 mm in two patients and 12.5 mm in one patient. Twelve implants were installed in three patients. Dental implants were simultaneously installed during removal of the distraction device in two patients. In one patient, the implant installation was delayed after device had been removed. All three patients showed the symptoms of mild to severe postoperative infection during the activation and consolidation. However, the distracted site showed undisturbed bone regeneration. Conclusion: The distraction osteogenesis showed the reliable results for the vertical augmentation of fibular bone which was used for the mandibular reconstruction. However, the great tendency of postoperative infection must be considered and clinically controlled.