• Title/Summary/Keyword: Regeneration rate

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High Frequency Protocorm-like Body(PLB) Formation through Root Cultures Doritaenopsis Hybrids(Orchidaceae) (Doritaenopsis 뿌리배양으로부터 고빈도의 Protocorm-like Body(PLB)형성)

  • Park, So-Young;Oh, Sung-Rae;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
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    • v.30 no.3
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    • pp.241-244
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    • 2003
  • Root cluster section culture, showing high efficient Protocorm-like body (PLB) formation capacity, were established in Doritaenopsis hybrids. Three types of root were obtained from excised shoots in 1/2MS medium containing different concentrations of NAA; \circled1normal roots, \circled2multiple roots and \circled3abnormal root clusters. Those were placed on 1/2MS medium supplemented with 0.5 mg/L thidiazuron for PLB regeneration. PLB regeneration rate was greater in root cluster section cultures (77.8%) compare to normal root tip cultures(30%). Number of PLBs regenerated from root cluster sections were counted over 11 per explant (5.3 per normal root tip).High frequency of PLB regeneration was achieved in root cluster section culture. This result can be used as an efficient method for clonal proliferation of Doritaenopsis hybrids.

Osmotic membrane distillation with continuous regeneration of stripping solution by natural evaporation

  • Gryta, Marek
    • Membrane and Water Treatment
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    • v.4 no.4
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    • pp.223-236
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    • 2013
  • The paper presents an experimental set-up for osmotic membrane distillation, which can be operated continuously for several weeks. NaCl solutions were used as an osmotic solution. The influence of osmotic solution concentration on the obtained permeate flux is presented. The experimental set-up was equipped with a system for the regeneration of dilute brine. The regeneration was carried out using a method of natural evaporation to the air surrounding the installation. The evaporation area was created by the Białecki rings, assembled in the form of tower. The obtained evaporation rate was sufficient to maintain a constant NaCl concentration (over 300 g/L), for air with the relative humidity in the range of 30-80%. Accurel PP S6/2 hydrophobic polypropylene membranes were used in the study. The membranes exhibited 100% rejection for 600 h of the process duration.

Rigorous Dynamic Simulation of PTSA Process (PTSA 공정의 상세 동적 모사)

  • Lee, Hye-Jin;Ko, Dae-Ho;Moon, Il;Choi, Dae-Ki
    • 제어로봇시스템학회:학술대회논문집
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    • 2000.10a
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    • pp.309-309
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    • 2000
  • The main objective of this study is to understand the regeneration step of the PTSA(Pressure and thermal swing adsorption) process below the atmospheric pressure by rigorous dynamic simulation. This target process is to recover toluene using activated carbon as an adsorbent. To do this, the dynamic simulations for the regeneration step are performed at 360, 490, 590mmHg and at high temperature after the simulation of the adsorption step at latm and 298K. A mathematical model was developed to simulate the column dynamics of the adsorption systems. This model is based on non-equilibrium, non-isothermal and non-adiabatic conditions, and axial dispersion and heat conduction are also considered. Heat transfer resistances are considered in gas-solid, gas-column wall and column wall-outside air. The LDF(Linear Driving Force) approximation model describes the mass transfer rate between the gas and solid phase. This study shows that the recovery of toluene by PTSA is more preferable than that by general TSA.

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Regeneration of Burner Type Diesel Particulate Trap System Through Active Exhaust Gas Feeding (배기 가스 유량 제어를 이용한 버너방식 디젤 입자상물질 제거 장치의 재생)

  • 김재업;박동선;이만복;김응서
    • Transactions of the Korean Society of Automotive Engineers
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    • v.5 no.1
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    • pp.1-13
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    • 1997
  • The key point that guarantees the durability of the ceramic monolith filter is to lower peak temperature and temperature gradient inside filter during regeneration. The control of the exhaust gas flow rate into the filter, by the bypass technique of the exhaust gas, enables the gas temperature in filter to be constant for regeneration. A couple of methods, which are the ON/OFF and PID control of the bypass valve, were used for feedback control of the gas temperature. These techniques showed that the ceramic filter was regenerated perfectly under the peak temperature and peak temperature gradient limitations for durability.

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Study on the Propagation System and the Photosynthetic Rate of Chrysantemum zawadskii H. (약용자원식물 구절초의 고소득화를 위한 번식체계 확립 및 재분화 식물체의 광합성 능력증대 I. 구절초의 기내배양 및 재분화 식물체의 RAPD 분석)

  • 김정률
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.1-8
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    • 1998
  • This study was conducted to establish mass propagation system from the axillary bud culture of chrysanthemum zawadskii H. which was used as material of medicinal plants. Shoot egeneration was better on MS medium with NAA and BA. The optimum concentraions of growth regulator for shoot regeneration differed depending on accessionsof C. Zawadskii. Shoot regeneration in Keungucheolcho was better on MS Medium with NAA 0.01mg/1 and BA 0.1mg/1 while Hyangrobonggucheocho was better with NAA 0.1mg/1and BA 0.3mg/1. Addition of NAA into medium was effective for induction of root from shoots regenerated. Shoot multiplcation was more effective when 10mg/1 spermine was added into medium than when other polyamines were treated ino medium . Randomly and specifically amplified polymorphic DAC banding patterns based on polymerase chain reaction (PCR) analysis were used to assess the genetic variation of plants regenerated from in vitro culture.

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Establishment of Efficient Regeneration System Through In Vitro Culture of Lettuce (Lactuca sativa)

  • Kim, Young-Sook;Kwon, Tea-Ho
    • Plant Resources
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    • v.2 no.1
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    • pp.16-21
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    • 1999
  • An efficient regeneration system was established by using in vitro plantlets of germinated seedlings from different cultivars of lettuce (Lactuca sativa cv. Chongchima, Chongchuckmyun, Jeokchima, Jeokchuckmyun). Shoot formation were observed from all cultivars on MS medium supplemented with 0.1 mg/L NAA and 0.5 mg/L BA. In all cultivars, when cotyledon was cultured, the number of shoot per explant was more greater than that hypocotyl and leaf disc were cultured. Shoot formation rate (91.7%) was high in a cotyledon culture of cultivar, Chongchukmyun. The growth of multiple shoots derived from the cultivar, Chongchukmyun, was most effective on medium containing 0.5 mg/L BA and 1.0 mg/L GA$_3$. When shoots were transferred on MS medium without plant growth regulators, roots were effectively differentiated. Rooted plantlets were acclimated on pots for further propagation.

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Adventitious Shoots Regeneration from Seed Explants of Xanthoceras sorbifolium

  • Hyunseok Lee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.58-58
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    • 2020
  • Xanthoceras sorbifolium Bunge (yellowhorn) is a woody tree in the soapberry family, Sapindaceae, native to northern China. This species has been identified as a major woody bioenergy plant for bio-diesel production because of high oil content in seed. But the flowers do not bear fruit well while the many flowers blooming. This study was performed to regenerate in vitro plantlet using adventitious shoot formation. To establish the protocol of plant regeneration, adventitious shoots formation rate in the culture of cotyledon of immature zygotic embryos was 68.6% in 1/2 MS medium with 0.5 mg l-1 BA and 3% sucrose (w/v). In the culture of cotyledons of mature zygotic embryos, induction of adventitious shoots was needed to contain high sucrose in pre-culture medium and the frequency of shoot induction was 64.4%. Multiple shoots were induced in 0.5 mg l-1 TDZ, and rooting of shoot was induced 4.0 mg l-1 IBA. Flow cytometry analysis revealed that all the regenerated plantlets were diploid.

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Evaluation of CO2 desorption efficiency of scrubber absorption system in Liquor Plant (주류공정 내 흡수시스템 CO2 탈리 효율 평가)

  • Park, Il-Gun;Park, Hyung-June;Lee, June-Hyung;Kim, Heung-Rae;Lee, Ju-Yeol
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.2
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    • pp.173-182
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    • 2020
  • In this study, amino acid salt absorbents were applied to remove and capture high concentrations of CO2 in liquor factories and regeneration performance was confirmed. In order to evaluate the effective treatment of the desorption process, two methods(Hot plate and Steam) were compared at the laboratory scale. As a result, hot plate and Steam. Hot plate methods regeneration efficiency was about 10% and Steam methods was about 60%. The Steam-Tower condition was evaluated by installing a 100㎥/min flow rate scrubber absorption system in the liquor factory. As a result, it was established that the absorbent flow rate was below 4L/min and the steam temperature was above 160℃. Finally regeneration performance was confirmed as 85.5%.

Effect of Electroacupuncture on the Regeneration of Injured Peripheral Nerve (손상된 말초신경의 재생에 미치는 전자침의 효과)

  • Deung, Young-Kun;Kim, Dong-Heui;Kang, Song-Jian
    • Applied Microscopy
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    • v.32 no.1
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    • pp.1-8
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    • 2002
  • To confirm the effect of electroacupuncture on the regeneration of injured peripheral nerve, the change of evoked potential in the sciatic nerve, the change of enzyme activity in the spinal cord, and morphological change of injured sciatic nerve were examined comparatively in acupuncture group (AG) and control group (CG) after sciatic nerve of guinea pig was injured by purpose. The value of evoked potential after injury of the sciatic nerve was increased in both AG and CG, but the increase rate of that was higher in AG than CG. Acid phosphatase activity of the spinal cord was increased in 1CG and 2AG, but shown are tendency to return to the normal state as time went by. Ultrastructural recovering rate of the injured sciatic nerve was higher in AG than CG. Also, there was developed only adipose tissue in sciatic nerve of AG. As mentioned above, the effect of electroacupuncture on the regeneration of injured peripheral nerve was confirmed experimentally by change of evoked potential, acid phosphatase and ultrastructure. Especially, the effect of electroacupuncture was appeared clearly in an early stage than other treatment stages.

Retrospective Clinical Study on Marginal Bone Loss of Implants with Guided Bone Regeneration (골유도재생술과 동시에 식립한 임플란트의 변연골 흡수량에 대한 후향적 고찰)

  • Park, Seul-Ji;Seon, Hwa-Gyeong;Koh, Se-Wook;Chee, Young-Deok
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.34 no.6
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    • pp.440-448
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    • 2012
  • Purpose: The purpose of this study was to evaluate marginal bone loss of the alveolar crest on implants with or without guided bone regeneration and variables that have influenced. Methods: The clinical evaluation were performed for survival rate and marginal bone loss of 161 endosseous implants installed with guided bone regeneration (GBR) in 83 patients from September 2009 to October 2010 in relation to sex and age of patients, position of implant, implant system, length and diameter of implant. Study group (n=42) implant with GBR procedure, control group (n=41) implant without GBR technique. Simultaneous GBR approach using resorbable membranes combined with autogenous bone graft or freeze-dried bone allograft or combination. Radiographic examinations were conducted at healing abutment connection and latest visit. Marginal bone level was measured. Results: Mean marginal bone loss was 0.73 mm in study group, 0.63 mm in control group. Implants in maxillary anterior area (1.21 mm) were statistically significant in study group (P<0.05), maxillary posterior area (0.81 mm) in control group (P<0.05). Mean marginal bone loss 1.47 mm for implants with diameter 3.4 mm, 0.83 mm for implants of control group with diameter 4.0 mm (P<0.05). Some graft materials showed an increased marginal bone loss but no statistically significant influence of sex, implant type or length. Conclusion: According to these findings, this study demonstrated the amount of marginal bone loss around implant has maintained a relative stable during follow-up periods. We conclude that implants with GBR had similar survival rate and crestal bone level compared with implants in native bone.