Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Protocorm-like Body(PLB) Formation through Root Cultures Doritaenopsis Hybrids(Orchidaceae)

Doritaenopsis 뿌리배양으로부터 고빈도의 Protocorm-like Body(PLB)형성

  • Park, So-Young (Plant Biotechnology Division, Forest Research Institute) ;
  • Oh, Sung-Rae (Horticulture Department, Chungnam National University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • 박소영 (임업연구원 생물공학과) ;
  • 오성래 (충남대학교 원예학과) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Published : 2003.09.01
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Abstract

Root cluster section culture, showing high efficient Protocorm-like body (PLB) formation capacity, were established in Doritaenopsis hybrids. Three types of root were obtained from excised shoots in 1/2MS medium containing different concentrations of NAA; \circled1normal roots, \circled2multiple roots and \circled3abnormal root clusters. Those were placed on 1/2MS medium supplemented with 0.5 mg/L thidiazuron for PLB regeneration. PLB regeneration rate was greater in root cluster section cultures (77.8%) compare to normal root tip cultures(30%). Number of PLBs regenerated from root cluster sections were counted over 11 per explant (5.3 per normal root tip).High frequency of PLB regeneration was achieved in root cluster section culture. This result can be used as an efficient method for clonal proliferation of Doritaenopsis hybrids.

Keywords

References

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