• 대한한방부인과학회지
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• 제33권2호
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• pp.1-12
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• 2020

Objectives: This study was conducted to find out the optimal extraction time for Artemisia argyi. Methods: The compositional pattern was compared with HPLC (High Performance Liquid Chromatography) and GC (Gas-Chromatography) by decocting Artemisia argyi 10, 60, 120 minutes respectively. Results: With longer extraction time, the contents of reference compounds were extracted 1.1 times more when 3,4-dicaffeoylquinic acid was extracted for 60 minutes than when extracted for 10 minutes in HPLC test, but the contents were reduced when extracted for 120 minutes compared to 60 minutes extraction time. 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, jaceosidin, and eupatilin showed the largest yield rate when extracted for 10 minutes, and it decreased as time passed. The contents of chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, jaceosidin, scoparone, and eupatilin were detected only in 10 minutes extraction but not in 60 or 120 minutes extraction according to GC test. Conclusions: The results show that extraction time could affect the physicochemical characteristic or composition of Artemisia argy extracted. Thus, short extraction time could be useful for decoction of Artemisia argyi.

• 한국광학회지
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• 제7권4호
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• pp.348-356
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• 1996

기체의 순간 온도를 측정할 수 있는 펄스 레이저를 사용한 이동형 CARS 분광기를 제작하였다. 이 분광기의 측정 프로그램은 측정한 스펙트럼으로 부터 온도를 얻기 위하여 8가지의 빠른 온도 계산법과 최소제곱법을 포함하고 있다. 빠른 온도 계산법 중 두 가지는 최소제곱법 보다 계산 시간은 훨씬 덜 걸리면서 온도의 분산이 작은 측정 결과를 준다. CARS 온도의 정밀 정확도는 복사온도계를 기준으로 흑연관 흑체로에서 측정하였다. 1000K부터 2400K의 온도 영역에서 정확도는 .+-.2% 이내이고 정밀도는 가장 정밀한 결과를 주는 빠른 온도 계산법을 적용할 때 1600K에서 .+-.35K이다. 순간 온도 측정의 적용 예를 보이기 위하여 이 분광기를 정해진 조건에 있는 난류 연소의 측정에 적용하였다.

• KIEAE Journal
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• 제14권2호
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• pp.77-86
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• 2014

Plenty of efforts have been made in the traditional architecture of Korea, Hanok, to develop various elements such as restoration, the introduction of new design, and energy-saving while systemic setups on standard and evaluation of eco-friendly energy design of Hanok are lacking. If we evaluate energy performance based on current standards without reflecting unique features of Hanok on the system, Hanok will be included in the very low grade among the residential buildings being included in the approval system of eco-friendly architecture or the unique features will be modified and the burden of increased construction cost. Therefore, this study is to prepare the basic reference for the introductory evaluation system by evaluating the energy performance level of NEO-Hanok based on the current building energy rating system. The result for NEO-Hanok based on the building energy rating system, we propose the rating standard with scorecard elements of NEO-Hanok by considering the necessity of identity and standard for NEO-Hanok. As a result of infiltration test to check the tightness, it was measured as 10.81 times/h (50 ACH). As we switch from the main insulation for the wall from the glass wool 64k(0.035W/mk) to rigid polyurethane foam first class first unit (0.024W/mk), the result was slightly increased from the first demand quantity rating yield $249.8kWh/m^2{\cdot}yr$ to $235.0kWh/m^2{\cdot}yr$. Current certificate system is focused more on the heating load than the cooling load, it is disadvantageous for Hanok, which has less cooling energy consumption in summer. The rating result from the target building study is level 4.

• 한국응용곤충학회지
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• 제10권2호
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• pp.91-95
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• 1971

본 시험은 우리나라에서 발생하는 푸른곰팡이병균의 종의 빈도와 발병환경 및 방제법을 구명코져 실시하였다. 시험결과 Trichoderma koningi, T. lignorum, T. glaucum과 미동정의 1종등 4종의 병원균이 분리되었고 이들의 빈도는 각각 $50\%\;32\%,\;13\%$와 $5\%$이었다. 푸른곰팡이병균은 감자배양액, 왁스만배양액과 리차드배양액에서 생육이 잘 되었으며 중성-염기성배지에서는 생육이 불량한 반면 산성에서 생육이 왕성하였으며 최적산도는 pH4였다. 양송이 수확기간 중 재배사내의 온도는 $15^{\circ}C$내외 일 때 본명의 발생이 적었고 수량이 많으며 $20^{\circ}C$이상에서는 본병의 발생이 격심하였다. 푸른곰팡이병균은 복토흙 소독시 $70^{\circ}C$에서 60분, 혹은 $80^{\circ}C$에서 30분간 열처리하므로서 완전히 사멸하였고 퇴비 후발효 과정에서도 사멸되었다.

• 대한의생명과학회지
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• 제13권1호
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• pp.33-38
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• 2007

The aim of this work was to validate a rapid and an accurate method for detecting Mycobacterium leprae in clinical specimens using nested PCR targeting M. leprae-specific repetitive element (RLEP) sequence. The primers were derived from the RLEP sequence which yield a 272 bp outer product and a 230 bp inner product. The specificity and the sensitivity of the nested PCR were compared with those of single PCR for detecting M. leprae using DNAs isolated from reference strain and various species of Mycobacterium. The results showed that the sensitivity of the nested PCR was about 100 to 1,000 times higher than that of the single PCR and also showed that both the single and the nested PCR were highly specific to M. leprae. Subsequently, the usefulness of the single and nested PCR was evaluated with clinical samples isolated from leprosy patients. The number of positive detections by the single and the nested PCR with a total of 20 specimens from leprosy patients were 9 (45%) and 20 (100%), respectively. The results clearly showed that nested PCR has highest sensitivity in detecting M. leprae from clinical specimens. Therefore, nested primers targeting RLEP sequence developed in this study seems to be useful to detect the presence of M. leprae.

• The Plant Pathology Journal
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• 제32권3호
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• pp.182-189
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• 2016

Together with the Fusarium graminearum species complex, F. culmorum is a major member of the causal agents of Fusarium head blight on cereals such as wheat, barley and corn. It causes significant yield and quality losses and results in the contamination of grain with mycotoxins that are harmful to humans and animals. In Korea, F. culmorum is listed as a quarantine fungal species since it has yet to be found in the country. In this paper, we report that two isolates (J1 and J2) of F. culmorum were collected from the air at a rice paddy field in Korea. Species identification was confirmed by phylogenetic analysis using multilocus sequence data derived from five genes encoding translation elongation factor, histone H3, phosphate permease, a reductase, and an ammonia ligase and by morphological comparison with reference strains. Both diagnostic PCR and chemical analysis confirmed that these F. culmorum isolates had the capacity to produce nivalenol, the trichothecene mycotoxin, in rice substrate. In addition, both isolates were pathogenic on wheat heads and corn stalks. This is the first report on the occurrence of F. culmorum in Korea.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권8호
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• pp.3312-3327
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• 2020

Plant diseases are a significant yield and quality constraint for farmers around the world due to their severe impact on agricultural productivity. Such losses can have a substantial impact on the economy which causes a reduction in farmer's income and higher prices for consumers. Further, it may also result in a severe shortage of food ensuing violent hunger and starvation, especially, in less-developed countries where access to disease prevention methods is limited. This research presents an investigation of Directional Local Quinary Patterns (DLQP) as a feature descriptor for plants leaf disease detection and Support Vector Machine (SVM) as a classifier. The DLQP as a feature descriptor is specifically the first time being used for disease detection in horticulture. DLQP provides directional edge information attending the reference pixel with its neighboring pixel value by involving computation of their grey-level difference based on quinary value (-2, -1, 0, 1, 2) in 0°, 45°, 90°, and 135° directions of selected window of plant leaf image. To assess the robustness of DLQP as a texture descriptor we used a research-oriented Plant Village dataset of Tomato plant (3,900 leaf images) comprising of 6 diseased classes, Potato plant (1,526 leaf images) and Apple plant (2,600 leaf images) comprising of 3 diseased classes. The accuracies of 95.6%, 96.2% and 97.8% for the above-mentioned crops, respectively, were achieved which are higher in comparison with classification on the same dataset using other standard feature descriptors like Local Binary Pattern (LBP) and Local Ternary Patterns (LTP). Further, the effectiveness of the proposed method is proven by comparing it with existing algorithms for plant disease phenotyping.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2493-2496
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• 2010

Erythropoietin (EPO) is mainly produced in kidney and stimulates erythropoiesis. The use of recombinant EPOs for doping is prohibited because of its performance enhancing effect. This study investigated whether biosimilar EPOs could be differentiated from endogenous one by iso-electro-focusing plus double blotting and SDS-PAGE for antidoping analysis. The established method was validated with positive control urine. The band patterns were reproducible and meet the criteria, which was made by world anti doping agency (WADA). Isoelectric focusing was conducted in pH range 2 to 6. Recormon (La Roche), Aropotin (Kunwha), Epokine (CJ Pharm Co.), Eporon (Dong-A), Espogen (LG Life Sciences), and Dynepo (Shire Pharmaceuticals) were detected in basic region. All biosimilars showed discriminative isoelectric profiles from endogenous EPO profiles, but they showed different band patterns with the reference one except Epokine (CJ Pharm Co.). Next, SDS-PAGE of biosimilar EPOs resulted in different molecular weight patterns which were distributed higher than endogenous EPO. Commercial immune assay kit as an immune affinity purification tool and immobilized antibody coated magnetic bead were tested for the purification and concentration of EPO from urinary matrix. The antibody-coated magnetic bead gave better purification yield. The IEF plus double blotting and SDS-PAGE with immunoaffinity purification method established can be used to discriminate biosimilar EPOs from endogenous EPO.

• 한국강구조학회 논문집
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• 제23권1호
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• pp.125-135
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• 2011

최근 타워형 아파트구조에 많이 사용되고 있는 무량판 구조시스템을 대신해 층고절감 및 장스팬 구현 그리고 내화성능향상을 목적으로 GFRP를 이용한 경량합성바닥을 개발하였다. GFRP를 이용한 경량합성바닥은 웨브에 개구부를 가지는 비대칭 강재보 하부에 GFRP를 부착하고 슬래브에 경량체를 삽입한 중공합성바닥이다. 이에 개발된 합성바닥의 휨성능을 평가하기 위해 GFRP, 중공률, 웨브의 개구부 등을 변수로 실대 실험을 수행하였다. 그 결과 GFRP를 이용한 합성보 실험체는 기준 실험체에 비해 휨내력 및 강성 측면에서 10% 높은 성능을 나타냈으며, 구조물이 항복할 때까지 완전합성거동하였다. 항복 이후 웨브개구부 주변의 응력집중현상에 의해 연성이 감소하는 현상이 나타났으며, 최대내력점까지 미끄러짐의 발생은 미소하였다. 내력설계 측면에서는 안전율을 고려해 해석값의 85%를 설계내력으로 평가하는 것이 타당한 것으로 나타났다.

• 한국콘텐츠학회논문지
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• 제9권3호
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• pp.312-319
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• 2009

보호관찰은 현재 범죄자의 낙인방지와 사회복귀를 위한 최선의 형사정책수단으로 인정되고 있다. 이러한 보호관찰의 본래의 취지와 이념을 효과적으로 달성하기 위해서는 보호관찰 대상자의 재범위험에 근거하여 대상자를 과학적으로 분류하여 재범의 가능성이 높은 대상자에 대하여는 감독의 수준을 높이고, 재범의 가능성이 낮고 개선의 의지가 있는 대상자에게는 지도와 원호를 통해 지역사회를 보호하면서 재범을 예방하며 범죄자의 재활을 도와야 할 것이다. 보호관찰 대상자에 대한 합리적 체계적인 분류관리방안을 수립하는 것은 분류에 따른 보호관찰 처우의 개별화를 도모하여 대상자의 건전한 사회복귀를 촉진시켜 범죄없는 안전한 사회로 이끌 것이다. 본 연구는 보호관찰대상자들 중 재범자와 비재범자가 어떠한 차이점이 있는지를 비교해봄으로써 처우 기법의 다양화를 모색할 수 있는 자료를 제시하는 것이라 하겠다. 즉 보호관찰대상자가 가진 여러 요인 중 어떠한 요인이 재범위험성과 관련이 있는지를 분석하며 나아가 대상자의 재범 가능성을 실질적으로 감소시킬 수 있는 정책을 제시할 수 있을 것이다.