• Title/Summary/Keyword: Redundancy analysis

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β-Galactosidase Gene of Thermus thermophilus KNOUC112 Isolated from Hot Springs of a Volcanic Area in New Zealand: Identification of the Bacteria, Cloning and Expression of the Gene in Escherichia coli

  • Nam, E.S.;Choi, J.W.;Lim, J.H.;Hwang, S.K.;Jung, H.J.;Kang, S.K.;Cho, K.K.;Choi, Y.J.;Ahn, J.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.11
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    • pp.1591-1598
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    • 2004
  • To isolate the $\beta$-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated strains, the strain of KNOUC112 produced the highest amounts of $\beta$-galactosidase at 40 h incubation time (0.013 unit). This strain was aerobic, asporogenic bacilli, immobile, gram negative, catalase positive, oxidase positive, and pigment producing. Optimum growth was at 70-72$^{\circ}C$, pH 7.0-7.2, and it could grow in the presence of 3% NaCl. The main fatty acids of cell components were iso-15:0 (30.26%), and iso-17:0 (31.31%). Based on morphological and biochemical properties and fatty acid composition, the strain could be identified as genus Thermus, and finally as Thermus thermophilus by phylogenetic analysis based on 16S rRNA sequence. So the strain is designated as Thermus thermophilus KNOUC112. A gene from Thermus thermophilus KNOUC112 encoding $\beta$-galactosidase was amplified by PCR using redundancy primers prepared based on the structure of $\beta$-galactosidase gene of Thermus sp. A4 and Thermus sp. strain T2, cloned and expressed in E. coli JM109 DE3. The gene of Thermus thermophilus KNOUC112 $\beta$-galactosidase(KNOUC112$\beta$-gal) consisted of a 1,938 bp open reading frame, encoding a protein of 73 kDa that was composed of 645 amino acids. KNOUC112$\beta$-gal was expressed as dimer and trimer in E. coli JM109 (DE3) via pET-5b.

A Hierarchical Image Mosaicing using Camera and Object Parameters for Efficient Video Database Construction (효율적인 비디오 데이터베이스 구축을 위해 카메라와 객체 파라미터를 이용한 계층형 영상 모자이크)

  • 신성윤;이양원
    • Journal of Korea Multimedia Society
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    • v.5 no.2
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    • pp.167-175
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    • 2002
  • Image Mosaicing creates a new image by composing video frames or still images that are related, and performed by arrangement, composition and redundancy analysis of images. This paper proposes a hierarchical image mosaicing system using camera and object parameters far efficient video database construction. A tree-based image mosiacing has implemented for high-speed computation time and for construction of static and dynamic image mosaic. Camera parameters are measured by using least sum of squared difference and affine model. Dynamic object detection algorithm has proposed for extracting dynamic objects. For object extraction, difference image, macro block, region splitting and 4-split detection methods are proposed and used. Also, a dynamic positioning method is used for presenting dynamic objects and a blurring method is used for creating flexible mosaic image.

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An On-demand Station Location Management scheme using 6-address structure of IEEE 802.11s (IEEE 802.11s 6-Address 구조를 이용한 On-demand 방식 단말 위치 관리기법)

  • Jang, Jeong-Hun;Kong, Jong-Min;Kim, Jong-Deok
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.4
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    • pp.829-836
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    • 2013
  • Wireless mesh networks(WMNs) are wireless backbone networks technology consisting of a multi-hop routers. Location management is essential to provide a service to the terminal in WMNs. IEEE 802.11s standards have two basic location management scheme for location management - the proxy registration procedure and the associated station discovery protocol. These basic schemes, however, suffer from serious drawbacks including redundancy control message, ineffective location information maintenance, additional delay time. This paper propose an on-demand station location management scheme using 6-address structure of IEEE 802.11s. Through analysis and experimental evaluation on simulation, we show that proposal scheme reduce control message and forwarding delay time.

An efficient method and performance analysis for burst synchronization/error detection using cyclic codes (순환코드를 이용한 효율적인 동기/에러 검출 방법 및 성능분석)

  • 최양호
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.21 no.8
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    • pp.2013-2022
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    • 1996
  • Cyclic Codes can be used for burs(or time slot) synchronization as well as error detection as that the overhead bits of the burst, which would be nessary to seperate burst synchronization and error detection systems, may be eliminated. In this paper a new method for combined burst synchronization and error detection is proposed which requires CRC decoding once only, while the previous method which inspects channel error after searching for burst synchronization requeires CRC decoding twice. The proposed method has the advantage of simple implementation and reducing processing time over the previous one, still showing the same detection perfdormance. It may occur that a burst different from the actually transmitted one is falsely accepted in the presence of channel errors. The exact expression for the false acceptance probability is newly presented through a simple derivation basied on the fact that it is determined by channel errors but not by detection methods.

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Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development

  • Park, Jun-Kyung;Lee, Seung-Hwan;Lee, Jang-Hoon;Han, Songhee;Kang, Hunseung;Kim, Jin-Cheol;Kim, Young Cheol;McSpadden Gardener, Brian
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.144-153
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    • 2013
  • Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

Analysis on the Dynamic Characteristics of a DDV Actuation System of a FBW Aircraft (FBW 항공기의 DDV 구동장치에 대한 운동특성 해석)

  • Nam, Yun-Su;Park, Hae-Gyun
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.34 no.3
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    • pp.74-80
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    • 2006
  • This paper deals with the control and fault monitoring of a DDV hydraulic actuation system. A hydraulic servo system has a nonlinear dynamics of an orifice flow through a valve spool. A full nonlinear model for a DDV actuation system is driven, and linearized to a simple model which is convenient for a control loop and fault monitor design. A top level requirement on the performance and safety for the actuation system is introduced. A control system and fault monitoring structure which can meet these requirements are discussed. A simulation package for a DDV actuation system which has a triplex redundant structure is developed.

A Fault Monitor Design for the Driving Currents of a DDV Actuation System of a FBW Aircraft (FBW 항공기의 DDV 구동장치에 대한 구동전류 고장 모니터 설계)

  • Nam, Yun-Su;Park, Hae-Gyun;;Choe, Seop;Gwon, Jong-Gwang
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.34 no.3
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    • pp.81-86
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    • 2006
  • This paper deals with a driving current fault monitor design methodology for a DDV actuation system which has a dual hydraulic power supply system, and triplex electric control capability. A fault existing among these redundant channels should be detected accurately and removed timely, and the remaining channels are to be reconfigured in order to compensate the role of a removed faulty channel. An integrated analysis on the aerodynamics, flight control laws, and DDV actuation system is essential for the design of an actuation system fault monitor. A method to define a fault transient boundary which specifies a maximum travel of an actuation system caused by the first faulty operation is proposed based on the top level requirement on the fault effect specified in MIL-F-8785C.

A Study on Reliability Evaluation Using Dynamic Fault Tree Algorithm (시스템 신뢰도 평가를 위한 동적 결함 트리(Dynamic Fault Tree) 알고리듬 연구)

  • 김진수;양성현;이기서
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.24 no.10A
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    • pp.1546-1554
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    • 1999
  • In this paper, Dynamic Fault Tree algorithm(DFT algorithm) is presented. This algorithm provides a concise representation of dynamic fault tolerance system including fault recovery techniques with fault detection, mask and switching function. And this algorithm define FDEP, CSP, SEQ, PAG gate which captures the dynamic characteristics of system. It show that this algorithm solved the constraints to satisfy the dynamic characteristics of system which there are in Markov and also this is able to satisfy the dynamic characteristics of system which there are in Markov and also this is able to covered the disadvantage of Fault tree methods. To show the key advantage of this algorithm, a traditional method, that is, Markov and Fault Tree, applies to TMR and Dual-Duplex systems with the dynamic characteristic and a presented method applies to those. He results proved that the DFT algorithm for solving the problems of the system is more effective than the Markov and Fault tree analysis model..

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Development of a Simplified Formula for the Damage Radius of a Naval Ship due to an AIR EXplosion (AIREX) (공기 중 폭발에 의한 함정의 손상반경 간이 계산식 개발)

  • Choi, Wan-Soo;Ruy, Won-Sun;Lee, Hyun Yup;Shin, Yun-Ho;Chung, Jung-Hoon;Kim, Euiyoung
    • Journal of the Society of Naval Architects of Korea
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    • v.57 no.4
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    • pp.207-212
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    • 2020
  • To decide a separation distance of the redundant vital equipment in a naval ship, the damage radius due to an aerial explosion should be estimated. In this research, a simplified formula for the damage radius has been developed by using existing empirical formulae for reflected shock pressure and shock lethality value of equipment. As a numerical example, the damage radius for a typical pump aboard a naval ship has been calculated by the developed formula and compared with the results calculated by Measure of Total Integrated Ship Survivability (MOTISS) which is one of survivability analysis codes verified, validated and accredited by the US Navy. Also, comparison with the results calculated by existing other simplified formulae has been made.

PMIPv6 Global Handover Mechanism using Multicast Source Based Forwarding (멀티캐스트 소스기반 포워딩을 이용한 PMIPv6 글로벌 핸드오버 메커니즘)

  • Choi, Hoan-Suk;Lee, Jang-Hyun;Rhee, Woo-Seop
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.7B
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    • pp.745-759
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    • 2011
  • In this paper, we propose the global handover mechanism that is able to provide the unlimited range of next-generation multimedia mobile services in an integrated environment. This mechanism consists of a multicast source based forwarding scheme and a global session management scheme. Global session management scheme provides LMA session information management, global mobility and route optimization. Multicast source based forwarding scheme delivers data between previously attached LMA and newly attached LMA without packet loss. In addition, this scheme removes the redundancy of buffered data. We present a performance evaluation and features analysis by the simulations using the ns-2. Global session management scheme has a less handover latency, propagation delay and signaling cost than the conventional methods. Multicast source based forwarding scheme can deliver buffer data without loss and it has less buffer size than conventional method.