• Title/Summary/Keyword: Reductive

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The Physiological and Biochemical Studies of Nocardia sp (Part 2) Tellurite-Reducing Enzyme (Nocardia sp의 생리 생화학적연구 (제2보) Tellurite 환원효소에 관하여)

  • 홍순덕
    • Microbiology and Biotechnology Letters
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    • v.5 no.3
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    • pp.141-151
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    • 1977
  • Distribution of tellurite and tellurate-reducing enzymes in the cell of Nocardia sp, the purifcation and the chemical properties of enzymes were investigated. Tellurite- and tellurate-reducing enzymes were located in the cytoplasm, but T. T. C. reduction part was in the cell membrane. Purification of tellurite- and tellurate-reducing enzymes was possible with the application of ammonium sulfate precipitation method and DEAE-Cellulose or CM-Cellulose column chromatographic method from the crude soluble part of the cell. On investigating the properties of purified enzyme, one of NADP, NADPH and reductive methylene blue(leucomethylene blue) was thought to react as a hydrogen donor. Both NADH and NADPH, or either of them would be physiological hydrogen donor.) In the reaction of this enzyme, either tellurite or tellurate reacts as a hydrogen acceptor, but on the other hand either selenate or selenate also reacts as a hydrogen acceptor.

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Research of Head Family and Ancestral Ritual Food's Conception Perceived by the Different Age Groups (연령에 따른 종가 및 제례음식에 대한 인식도 조사)

  • Kim, Mi-Hye;Chung, Hae-Kyung
    • Journal of the Korean Society of Food Culture
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    • v.29 no.6
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    • pp.488-498
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    • 2014
  • The research aimed to provide accurate and basic data comparing different perceptions of head family's food and food for religious ceremonies depending on age with the goal of sustaining traditional Korean food in the future. The gender distribution of the participants was 274 Males (42.5%) and 370 Females (57.5%). Age distribution was 211 participants (32.7%) in their 20s, 215 participants (33.3%) in their 30 to 40s, and 220 participants (34%) in their 50 to 60s. The older generation appeared to beaware of the definition of head family or 'The eldest's house of head family' as well as the concept of one's family five generations ago with more reductive and emphasis than imaginary concept of head family of the younger generation. The image of the head family was perceived as 'head family's food' in younger generations and as 'eldest son' in older generations. Family role and meaning most often manifested as 'succession of tradition' and 'cultural symbols' in younger generations, respectively, whereas older generations responded 'hallmark of the head family'. Family ancestral rites and head family's food had positive effects on awareness of head family's food. Moreover, those with experience in practicing family ancestral rites responded that head family's food should be more popular. People who viewed family's food more positively were more open with the idea thathead family's food could go mainstream. In conclusion, positive perception of head family's food and traditional pride are crucial environmental factors in public support of popularizing head family's food to the public.

Preparation of NaxWO3 (x= 1 and 0.75) Thin Films and Their Electrical Conduction Properties (NaxWO3 (x= 1, 0.75) 박막 제조 및 전기전도 특성)

  • Lee, Seung-Hyun;Sun, Ho-Jung
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.25 no.8
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    • pp.602-610
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    • 2012
  • The powders for the $Na_xWO_3$ (x= 1 and 0.75) sputtering targets were synthesized by the calcination in reductive atmosphere. Near single-phase $NaWO_3$ and single-phase $Na_{0.75}WO_3$ powder targets were prepared. By using the targets, thin films of each composition were deposited by rf magnetron sputtering on the $SiO_2$ (100 nm)/Si substrates and annealed by RTP (rapid thermal processing) for crystallization. In the case of the $NaWO_3$ composition, single-phase $Na_xWO_3$ thin films, where x was believed to be slightly less than 1, were fabricated accompanying the Na-diffusion into the substrates during RTP. However, in the case of the $Na_{0.75}WO_3$ thin film preparation, it was unable to make single-phase thin films. From the phase formation behaviors of both powders and thin films, it was revealed that $Na_xWO_3$ with nonstoichiometric composition of x, which was slightly less than 1, was favorable. The good electrical conduction properties were obtained from the single-phase $Na_xWO_3$ thin films. Their electrical resistivities were as low as $7.5{\times}10^{-4}{\Omega}{\cdot}cm$.

Reductive Leaching of $LiCoO_2$in a Sulfuric Acid Solution (황산용액서 $LiCoO_2$의 환원침출)

  • 이철경;김낙형
    • Resources Recycling
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    • v.10 no.6
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    • pp.9-14
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    • 2001
  • A sulfuric acid leaching of $LiCoO_2$as cathodic active materials of lithium ion secondary batteries was investigated in terms of reaction variables. In the absence of a reducing agent, the extraction of cobalt was less than 40% in 2 M sulfuric acid at $75^{\circ}C$ instead of that of lithium could be almost 100% in the same conditions. To improve the Co extraction, hydrogen peroxide was used as a reducing agent in the range 2~20 vol%. When over 10vo1% hydrogen peroxide was added, the extractions of both metals were improved to about 95%. It seems to be due to the reduction of Co(III) to Co(II) that can be readily dissolved. The extractions of Co and Li were increased with increasing $H_2$$SO_4$concentration and temperature, and amount of hydrogen peroxide and with decreasing of pulp density. The optimum leaching conditions were determined at $2 M H_2$$SO_4$concentration, $75^{\circ}C$ operating temperature, 100 g/L. initial pulp density, 20 vol% $H_2$$O_2$addition and 30 min.

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Equilibrium calculations for HyBRID decontamination of magnetite: Effect of raw amount of CuSO4 on Cu2O formation

  • Lee, Byung-Chul;Kim, Seon-Byeong;Moon, Jei-Kwon
    • Nuclear Engineering and Technology
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    • v.52 no.11
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    • pp.2543-2551
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    • 2020
  • Calculations of chemical equilibrium for multicomponent aqueous systems of the HyBRID dissolution of magnetite were performed by using the HSC Chemistry. They were done by using a Pitzer-based aqueous solution model with the recipe of raw materials in experiments conducted at KAERI. The change in the amounts of species and ions and the pH values of the solution at equilibrium was observed as functions of temperature and raw amount of CuSO4. Precipitation of Cu2O occurred at a large amount of CuSO4 added to the solution, while no precipitation of Cu(OH)2 was found at any amounts of CuSO4. The E-pH diagrams for Cu were constructed at various Cu concentrations to provide the effect of the Cu concentration on the pH values at boundaries where the coexistence of Cu+ ion and Cu2O solid occurred. To prevent Cu+ ions from being precipitated to Cu2O, the raw amount of CuSO4 should be adjusted so that the pH value of the solution from the equilibrium calculation is less than that from the E-pH diagram. We provided guidelines for the raw amount of CuSO4 and the pH value of the solution, which prevent the formation of Cu2O precipitates in the HyBRID dissolution experiments for magnetite.

Microbial Community in the TPH-Contaminated Aquifer for Hot Air Sparging using Terminal-Restriction Fragment Length Polymorphism (유류오염대수층 고온공기분사공정시 제한효소다형성 미생물 군집)

  • Lee, Junho;Park, Kapsong
    • Journal of Korean Society on Water Environment
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    • v.24 no.1
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    • pp.19-29
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    • 2008
  • Hot air sparging is a groundwater remediation technique, in which organic contaminants volatilized into hot air from the saturated to vadose zone. In the laboratory diesel (10,000 mg TPH/kg) was spiked in contaminated saturated aquifer soil. The hot air ($34.9{\pm}2.7^{\circ}C$) was injected in intermittent (Q=1,500 mL/min, 10 minute injection and 10 minute idle) modes. We performed microcosm tests using the groundwater samples to assess TPH reductive remediation activity. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for hot air sparging experiment with sludge soil samples that were closely related to Bacillus (149 bp, Firmicutes), Methlobacterium (149 bp, Euryarchaeotes), Pseudomonas (492 bp, ${\gamma}$-Proteobacteria), etc., in the clone library. In this study we find that TPH-water was reduced to 78.9% of the initial value in this experiment aquifer. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil fate of microorganisms in natural microbial community.

Reduction of Nitrate-nigrogen by Zero-valent Iron Adhered in Mesoporous Silicas (메조기공 실리카에 부착된 영가철을 이용한 질산성 질소의 환원)

  • Yeon, Kyeong-Ho;Lee, Seunghak;Lee, Kwanyong;Park, Yong-Min;Kang, Sang-Yoon;Lee, Jae-Won;Choi, Yong-Su;Lee, Sang-Hyup
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.1
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    • pp.139-147
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    • 2007
  • For environmental remediation of a contaminated groundwater plume, the use of zero-valent metal represents one of the latest innovative technologies. In this study, the effects of denitrification by zero-valent iron adsorbed in mesoporous silicas have been studied for groundwater contaminant degradation. The mesoporous silica was functionalized with 3-mercaptopropyltrimethoxysilane (MPTS) ligands and the zero-valent iron precipitated in the mesopore of granular silica was made by $FeCl_2$ and $NaBH_4$. Hydrogen was exchanged with $Fe^{2+}$ ions in the granular silicas. And then the ions were reduced by sodium borohydride in the mesoporous silicas. The surface area of the silica determined via the BET method ranged from 858 to $1275m^2/g$. The reductive reaction of nitrate-nitrogen indicated that the degradation of nitrate-nitrogen appeared to be pseudo first-order with the observed reaction rate constant kobs ($0.1619h^{-1}$) and to be directly proportional to the specific surface area. Therefore, the mesoporous silica with nano zero-valent iron proposed as a novel treatment strategy for contaminated groundwater was successfully implemented herein for the removal of nitrate-nitrogen.

Development of a Diagnosis Algorithm of Influent Loading Levels Using Pattern Matching Method in Sequencing Batch Reactor (SBR) (연속회분식반응기에서 패턴매칭방법을 이용한 유입수 부하수준 진단 알고리즘 개발)

  • Kim, Ye-Jin;Ahn, Yu-Ga;Kim, Hyo-Su;Shin, Jung-Phil;Kim, Chang-Won
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.2
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    • pp.102-108
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    • 2009
  • DO, ORP and pH values measured during SBR operation can provide information about removal reaction of organic contaminants and nutrient materials in the reactor. It is already generalized control strategy to control reaction phase time using their special patterns indicating the end of the removal reactions. However, those informations are limited to point out the end time of oxidative reaction in the aerobic phase or reductive reaction in the anoxic phase without giving quantitative value of influent loading level. In this research, a diagnosis algorithm which can estimate the loading level of carbon and ammonia as high, medium and low was developed using the basic measurements like DO, ORP, and pH. It will be possible to know the level of influent loading rate from those online measurements without experimental analysis.

Reproductive Cycle of the Red Marbled Rockfish Sebastiscus tertius (붉은쏨뱅이(Sebastiscus tertius)의 생식 주기)

  • Lim, Sang-Gu;Kim, Kawang-Su;Kim, Chul-Won;Kim, Jung-Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.701-708
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    • 2011
  • The aim of the present study was to analyze the reductive cycle of the red marbled rockfish Sebastiscus tertius. The analysis was based on annual changes in the gonadosomatic index (GSI), the hepatosomatic index (HSI), histology of the gonadal structure, and plasma sex steroid hormone levels of adult fish from April 1997 to April 1998. GSI of females began to increase in February and peaked ($10.8{\pm}2.72$) in May. HIS levels ($3.41{\pm}0.49$) peaked in February and elevated plasma steroid hormones ($1.47{\pm}0.75$ ng/mL for estradiol-$17{\beta}$ ($E_2$) and $230.7{\pm}27.6$ pg/mL for testosterone (T)) were observed in April. However, in male fish, GSI levels started to increase in August and remained high until November ($0.21{\pm}0.05$). T levels were was also elevated in August and peaked in October ($188.1{\pm}43.5$ pg/mL) and November ($186.8{\pm}28.0$ pg/mL), but started to decline 1 month than the GSI. These results suggest that female ovoviviparious periods span from April to June and amle mating periods occur from November to February.

Study on the effect of glucose upon the digestibility in the perilla frutescens leave (들깨엽(葉) 단백질(蛋白質)의 소화(消化)에 있어서 glucose의 영향(影響)에 관(關)한 연구(硏究))

  • Kim, Hong-Sub
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.4 no.1
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    • pp.31-34
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    • 1975
  • The testing materials which kept perilla frutescens' leaves frezen are divided into four parts, 1. freezing green leaves, 2. glucose added to the leaves dried in the sun, 3. glucose unadded to the leaves dried in the sun and 4. the leaves dried in the immediately after collecting sample. The perilla frutescens' leaves are treated with the artificial digestion test to investigate the effects of the digestibility of ingredients and of protein. The results obtained were as follows ; 1. The digestibility of crude protein of sample using the common leaves dried in the sun immediately after collecting sample was presented highest at 83.15%, the freezing green leaves at 68.35%, glucose added to the leaves dried in the sun at 64.25% and glucose unadded to the leaves dried in the sun at 62.12%. The digestibility of perilla frutescens' by freezing green leaves, glucose added or glucose unadded to the leaves dried in the sun is on the decrease without difference. 2. It was suggested that glucose and reductive sugars to perilla frutescens' leaves is not affected by the decreased digestibility of protein, dince the digestibility of glucose added to the leaves dried in the sun and glucose unadded to the leaves dried in the sun almost never makes a difference. 3. The digestibility of freezing the green leaves for six months was quite different from the leaves that were dried in the sun immediately after collecting sample, in that the leaves that were frozen for six months were decreased 1/5 quantity of the shole crude protein.

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