• Korean Journal of Poultry Science
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• v.37 no.3
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• pp.283-287
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• 2010

In the present study we report in vivo inhibitory potential of single strain of bacteriophage ($CJ{\phi}07$) in day-old SPF chicks experimentally infected with Salmonella enteritidis (SE). The bacteriophages prepared by feed additives and drinking water were given to chicks for 20 days starting prior 10 days before challenge with SE. Chicks were euthanized at 10 days after challenge for quantitative salmonella isolation from intestine and determination of environmental contamination level of salmonella. Bacteriophage therapy as additives in feed and drinking water resulted in significant inhibition of the SE replication in intestines of SPF chickens (P<0.05). In addition, environmental contamination by SE fecal shedding was decreased in bacteriophage-treated birds. Therefore, bacteriophage $CJ{\phi}07$ examined in this study may be a plausible alternative to antibiotics for the reduction of salmonella infection both in poultry.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.404-410
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• 2009

We investigated the physicochemical stabilities and biological activities of ethanol- extracted pigment from marine bacteria Pseudoalteromonas sp. Ju11-1 and Pseudoalteromonas sp. Ju14. The bacterial pigment of strain Ju11-1 was very stable at pH 5.0 below $25^{\circ}C$. The stability of the pigment showed higher stability in the presence of metal ions such as $Cu^{2+}$ and $Mg^{2+}$. The pigment has activity of free-radical scavenging ($IC_{50}$ $95.2{\mu}g$/ml) and the protective antioxidant effect ($ED_{50}$ $82.3{\mu}g$/ml) against DNA damage in human lymphocytes. The bacterial pigment of strain Ju14 was very stable at pH range between 4.0 and 8.0 below $40^{\circ}C$. In the presence of light, the pigment was also very stable, showing more than 90 percent of remaining absorbance during 14 days at $25^{\circ}C$. The stability of the pigment, when metal ions were present, showed higher stability in all examined metal ions except for $Fe^{2+}$, $Al^{3+}$, and $Cu^{2+}$, especially in the presence of $Na^+$. The pigment has activity of freeradical scavenging ($IC_{50}$ $208.6{\mu}g$/ml) and the protective antioxidant effect ($ED_{50}$ $ 96.4{\mu}g$/m) against DNA damage in human lymphocytes. The result indicates that the bacterial pigments from marine bacteria, Pseudoalteromonas sp. Ju11-1 and Pseudoalteromonas sp. Ju14 showed higher physicochemical stability and significant effects for reduction in oxidative DNA damage. Therefore, the results suggest that these bacterial pigments could be used as a natural colorant having the advantages of antioxidant.

• Applied Biological Chemistry
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• v.32 no.4
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• pp.424-434
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• 1989

The rationale for the use of fungi in treating waste streams from food processing plants I~as been that of incorporating the dissolved and suspending nutrients into a macroscopic organism which can be filtered out readily. In order for a process using fungi to meet these objectives we examined a strain of fungi, Aspergillus fumigatus, which grew well on a variety of polysaccharide-containing materials and showed both efficient BOD removal and high quality protein recovery. In this experiment the fungal choice was based on the laboratory screening studies where the criteria used was BOD and COD reduction, growth response, mycelial yield, and the ability to compete with the natural flora. In the fermentation system used far the continuous culture of Aspergillus fumigatus the best combination of operating variables, inoculum ratio, temperature, initial pH, fermentation time and agitation rate was 5%(v/v), $35{\sim}40^{circ}C,\;pH\;4.5{\sim}5.0$, 2days and 150rpm, respectively. The fungus reduced BOD and COD to 94.0 and 90.4%, respectively and 3.15g of dry mycelium per liter of alcohol waste was harvested during 48hr of incubation time. The protein efficiency ratios for the control diet and the experimental diet containing the fungal protein were $3.42{\pm}0.15$ and $3.40{\pm}0.43$, respectively.

• International Journal of Highway Engineering
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• v.13 no.2
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• pp.159-166
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• 2011

This research was performed to evaluate physical properties of polysulfide epoxy overlay material for bridge deck as part of a review for possibility of domestic application of polymer concrete for bridge deck pavement. In order to evaluate strength characteristics, compressive strength, flexural strength and bond strength were tested, and, for durability characteristics, chloride ion penetration resistance and freeze/thaw resistance were tested along with ultraviolet rays impact evaluation. The tests showed that the results met the criteria suggested by the American Concrete Institute in terms of compressive strength, flexural strength and bond strength. However, in terms of the strengths measured at various test temperatures, it was found that the epoxy material was highly dependent on temperature, and, therefore, this should be considered at the time of domestic application of the epoxy material later. Deflection characteristics was checked through flexural strength test and it was found that bridge deck pavement using the epoxy material was excellent compared to bridge deck pavement using asphalt. Furthermore, the results of chloride ion penetration resistance test and freeze/thaw resistance test were also excellent. In the evaluation of ultraviolet rays impact on epoxy slurry mixture, reduction of strain was noticed with increased strength, but the deflection characteristics after exposure to ultraviolet rays was better than the existing acryl polymer concrete. Therefore, it is concluded from the research that the polysulfide epoxy overlay material has the physical properties that are appropriate to pavement of bridge deck.

• Food Science of Animal Resources
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• v.26 no.2
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• pp.263-268
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• 2006

Probiotics including Lactobacillus acidophilus, refer to a group of nonpathogenic organisms that protect the human host against gastrointestinal(GI) infections by pathogenic bacteria such as Shiga toxin-producing E. coli(STEC). In the study, the inhibitory effects of STEC ATCC 43894 adhesion by L. acidophilus A4 was investigated on the HT-29 epithelial cells. Specific proteins regulated by cell Iysates of L. acidophilus A4 on STEC ATCC 43894 were also characterized by proteomic analysis. Both cell mass and Iysate of L. acidophilus A4 have exhibited the profound inhibitory activity on the HT-29 cells(about 1.5 log scale reduction). Two-dimensional gel electrophoresis(2-DE) revealed seven proteins that were up-regulated by cell Iysates of L. acidophilus A4 and three proteins that were down-regulated. In addition, three protein spots were only detected in the presence of cell Iysates. These results suggest that inhibitory effects of STEC adhesion by L. acidophilus may be due to the regulation of specific protein of STEC.

• Journal of the Korean Geotechnical Society
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• v.28 no.11
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• pp.69-78
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• 2012

Conventional numerical analysis for rainfall-induced slope stability has been estimated by separating seepage and stress-strain behavior, respectively. Many researchers' models from commercial softwares and literatures define that partially saturated permeability is the only function of degree of saturation (or matrix suction) and then they do not consider hydraulic-mechanical characteristics for the analysis. However, in practice, the water flow processes in a deformable soil are influenced by soil skeleton movement and the pore water pressure changed due to seepage will lead to changes in stress and to deformation of a soil. The relationship between seepage and soil behavior causes a change of partially saturated permeability as well as saturated permeability with the lapse of time. Instability of partially saturated soil slopes due to infiltration would be analyzed from reduction of negative pore water pressure calculating the process of water flow based on predicted partially saturated permeability. Therefore, partially saturated permeability should be defined by the function of degree of saturation (or matric suction) and porosity. The paper presents the comparison between staggered and monolithic coupled analysis regarding seepage and stress deformation problems. As a result, the decrease in matric suction on soil slope from monolithic analysis is slower than that from staggered analysis.

• Journal of the Korea Concrete Institute
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• v.13 no.1
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• pp.1-8
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• 2001

Recently, prestressed concrete(PSC) bridge structures with many repetitive spans have been widely constructed using the segmental construction method in many countries. In these segmentally constructed PSC bridges, there exist many construction joints which is required coupling of tendons or overlapping of tendons to introduce continuous prestress through several spans of bridges. The purpose of this paper is to investigate in detail the complicated stress distributions around the tendon coupled joints in prestressed concrete girders. To this end, a comprehensive experimental program has been set up and a series of specimens have been tested to identify the effects of tendon coupling. The present study indicates that the longitudinal and transverse stress distributions of PSC girders with tendon couplers are quite different from those of PSC girders without tendon couplers. It is seen that the longitudinal compressive stresses introduced by prestressing are greatly reduced around coupled joints according to tendon coupling ratios. The large reduction of compressive stresses around the coupled joints may cause deleterious cracking problems in PSC girder bridges due to tensile stresses arising from live loads, shrinkage and temperature effects. The analysis results by finite element method correlate very well with test results observed complex strain distributions of tendon coupled members. It is expected that the results of this paper will provide a good basis for realistic design guideline around tendon coupled joints in PSC girder bridges.

• Applied Biological Chemistry
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• v.42 no.3
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• pp.188-192
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• 1999

In order to minimize the mycelial pellet formation, one of the critical obstacles during the fermentation processes of filamentous fungi, an investigation was focused on the culture conditions(media and initial inoculum) and additives(soils, surfactants and polyethylene glycol 200) when a high phosphate-dissolving fungus, Penicillium sp. GL-101, was cultured in liquid media. Culturing the strain in PDB, SDB and YPD media, their pellet sizes decreased to the order of YPD > SDB > PDB. And at the high concentrations of the initial inoculum in the range from $1{\times}10^3\;to\;1{\times}10^6$ conidia/ml, the small sizes of pellet were formed in the PDB media. For the initial inoculum between $1{\times}10^7\;and\;1{\times}10^8$ conidia/ml, however, an amorphous pellet or loose aggregate was formed. The addition of soils, zeolite and diatomite, up to 1.0% decreased the pellet sizes to 3/4 and 1/2, respectively, but the pellet was increased to 2.5 times by the addition of bentonite. Surfactants also affected on the size of pellet; the addition of Triton X-100 and Tween 80 up to 1.0% decreased the pellet sizes maximally to 1/10 and 1/4, respectively, while SDS completely inhibited the fungal growth. Among the four additives tsted, polyethylene glycol 200 was the most effectively reduced the pellet sizes to $0.2{\pm}0.1$mm that resulted in about 25- fold reduction compared to the control.

• Computational Structural Engineering
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• v.10 no.4
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• pp.165-175
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• 1997

In this paper, the stress, buckling and vibration analyses have been performed for several case with the spot weld stiffened rear side frame, the unstiffened rear side frame and the dual thickness laser weld rear side frame. For stress and vibration analyses, the clamped boundary condition with spring supports are used. But for the buckling analyses, the both ends simply supported boundary conditions are used. For the nummerical analyses, ANSYS 5.0 code is adopted. Maximum stress of the spot weld stiffened rear side frame occurs in the main frame and is 80.9 MPa. Maximum strain is 501 .mu.. The maximum stress of the dual thickness laser weld rear side frame of 1.8mm thickness structure is equal with the stress of spot weld stiffened frame. The weight of dual thickness laser weld frame can be reduced about 17.2%. For the stiffened spot weld rear side frame with both ends simply supported boundary conditon, the bucking load is 52.54 kN. When the thickness of the dual thickness laser weld rear side frame become 1.9mm thickness structure, the buckling load of the stiffenerd rear side frame is equal to that of dual thickness laser weld frame. The reduction of the structure weight is about 5%. The fundamental natural frequency of the stiffened spot weld rear side frame for bending mode is 163.6 Hz and that of the dual thickness laser weld rear side frame is 179.8 Hz.

• Journal of the Korean Geotechnical Society
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• v.35 no.7
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• pp.15-27
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• 2019

The purpose of this study is to investigate the mechanism about damages occurring at quay wall and backfill in Youngilman Port during Pohang earthquake (M5.4) on November 15, 2017. In the field investigation, the horizontal displacement of the caisson occurred between 5 cm and 15 cm, and the settlement at backfill occurred higher than 10 cm. 2D-effective Stress Analysis was performed to clarify the mechanism for the damage. The input earthquake motion used acceleration ($3.25m/s^2$) measured at bedrock of Pohang habor. Based on a numerical analysis, it was found that the effective stress decreased due to the increase of excess pore pressure in the backfill ground and the horizontal displacement of the caisson occurred by about 14 cm, and the settlement occurred by about 3 cm. In backfill, the settlements occurred between 6 cm and 9 cm. This is similar to field investigation results. Also, it was found that the backfill soil was close to the Mohr-Coulomb failure line due to the cyclic loading from the effective stress path and the stress-strain behavior. It may be related to decreasing of bearing capacity induced by the reduction of effective stress caused by the increase of the excess pore water pressure.