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Improving Endoglucanase Activity by Adding the Carbohydrate-Binding Module from Corticium rolfsii

  • Tang, Zizhong;Chen, Hui;Chen, Lijiao;Liu, San;Han, Xueyi;Wu, Qi
    • Journal of Microbiology and Biotechnology
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    • v.24 no.4
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    • pp.440-446
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    • 2014
  • The carbohydrate-binding module (CBM) is an important domain of most cellulases that plays a key role in the hydrolysis of cellulose. The neutral endoglucanase (EG1) gene was reconstructed. A redesigned endoglucanase, named EG2, was constructed with a CBM containing a linker from Corticium rolfsii (GenBank Accession No. D49448). The redesigned EG genes were expressed in Escherichia coli, and their characteristics are discussed. Results showed that the degradation of cellulose by EG2 was about double that by EG1. The specific activities of EG1 and EG2 were tested under optimal conditions, and EG2 had higher activity ($169.1{\pm}2.74$ U/mg) toward CMC-Na than did EG1 ($84.0{\pm}1.98$) in the process of cellulose degradation. The optimal pH and temperature, pH stability, and heat stability of EG1 and EG2 were similar. Results indicated that the CBM plays an essential role in the hydrolysis of cellulose. We can improve EG's catalytic power by adding the CBM from Corticium rolfsii.

A Walking Vibration Sensing System using a Fiber Bragg Grating Sensor (광섬유 브래그 격자 센서를 이용한 보행 진동 측정 시스템에 관한 연구)

  • Kim, Jaeki;Yeom, Sanghun;Lee, Seoksoon
    • Journal of Aerospace System Engineering
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    • v.11 no.1
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    • pp.22-27
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    • 2017
  • In this paper, a walking vibration sensing system (WVS system) using a Fiber Bragg Grating sensor (FBG sensor) is proposed. The seismic part of the FBG sensor was redesigned for sensitivity enhancement. The external excitation was assumed to be the walking cycle of an adult male. The FBG seismic sensor was redesigned using CATIA and ABAQUS such that the sensor's first mode natural frequency is 3.5 Hz (which is a value near the external excitation frequency). Compared with existing walking vibration sensing systems, this newly created system improves sensitivity 15 times. It is also suitable for intrusion detection applications.

Cost Scaling Factor according to Power Plant Capacity Change (발전소 용량변경에 따른 비용보정계수)

  • Ha, Gak-Hyeon;Kim, Sung-Hwan
    • Journal of Energy Engineering
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    • v.22 no.3
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    • pp.283-286
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    • 2013
  • The existing nuclear power plants have been often redesigned by increasing or decreasing electrical power without changing design concept by the request of utility, economic factors or other factors. When the cost of power plant equipment redesigned by changing reactor power and electrical power is estimated, if its quotation is not available in the market place, cost scaling factor(CSF) applies to the cost of existing plant equipment and then the new-designed equipment cost can be calculated. In this paper, we review CSFs according to plant capacity change cases in United State DOE, EPRI, ABB, SWEC and introduce the results applied to Korean PWR 1000MWe and 1400MWe.

Language and Symbolic Reference in Whitehead′s Philosophy (화이트헤드의 언어 이해와 상징적 연관)

  • 문창옥
    • Lingua Humanitatis
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    • v.6
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    • pp.147-166
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    • 2004
  • Whitehead's discussion of language is not to be found in any one book or article. It is interwoven with his discussion of many other questions. He was, however, greatly concerned with the problem of symbolism in general and the uses of language. He regards language, spoken or written, as an instrument devised by men to aid them in their adjustment to the environment in which they live Language is used for many specific purposes in the process of this adjustment. Words are employed not only to refer to data and to express emotions. They may be used also to record experiences, and thoughts about these experiences. Worts also function as instruments in the organization of experiences as they are considered in retrospect. Thus words free us from the bondage of the immediate. And Whitehead's theory of meaning is implicit in his discussion of the functions of language. According to him, the human mind is functioning symbolically when some components of its experience elicit consciousness, beliefs, emotions, and usages, respecting other components of its experiences. The former set of components are the 'symbols', and the latter set constitute the 'meaning' of the symbols. Whitehead points out that one word may have several meanings, i.e. refer to several different data. In order to understand, thus, the meaning to which a word refers, it is sometimes very important to appreciate the system of thought within which a person is operating. Further, Whitehead's discussion of language includes a number of cogent warning the deficiencies of language, and hence the need for great care in the use of words. In fact, language developed gradually. For the most part we have created words designed to deal with practical problems. Attention focuses on the prominent features in a situation, in particular the changing aspects of things. With reference to such data our words are relatively adequate. However, this issues in an unfortunate superficiality. The enduring, the subtle, the complex and the general aspects of the universe do not have adequate verbal representation. for this reason, Whitehead's position concerning the uses of language in speculative philosophy is stated with pungent directness. The uncritical trust in the adequacy of language is one of the main errors to which philosophy is liable. Since ordinary language does not do justice to the generalities, profundities and complexities of life, it is obvious that philosophy requires new words and phrases, or at least the revision of familiar words and phrases. Proceeding to develop the theme Whitehead contends that words and phrases must be stretched towards a generality foreign to their ordinary usage. In the same vein Whitehead refers to the need to realize that language which is the tool of philosophy needs to be redesigned just as in physical science available physical apparatus needs to be redesigned. But even these words and phrases, stretched or redesigned, are never completely adequate in philosophical speculations. They are, in his opinion, merely a great improvement over ordinary language or the language science, mathematics or symbolic logic.

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An Evaluation of TOD Effect with the Concept of 'Pedestrian Traffic Ratio' ('보행율' 개념을 이용한 TOD 효과 평가)

  • Lee, Sang-Hyun;Fang, Lin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.763-772
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    • 2019
  • Recently, there is a great expectation for the sustainable effect of TOD and apply it to urban planning and design. However, there are few research on the quantitative evaluation method of the effect. The purpose of this study is to propose a quantitative way to assess the effectiveness of TOD(Transit Oriented Development)'s vehicle traffic reduction in the urban planning phase, based on the land-use plan. Firstly, it proposes a method to convert the information contained in the land-use plan into a network and secondly a method to estimate the travel distance based on the network. In the first phase, the roads in the land-use plan are converted into links and the intersection of the roads into nodes. The area and the height of the zones included in the land-use plan are converted into the weights on adjacent nodes. The second phase assumes the frequency at which travel occurs between nodes on the network is relative to the weights and estimates the travel distance by dividing it into a pedestrian and a vehicle. Then, taking the city without TOD as the object, through comparison present city and alternatively redesigned city to the analysis of TOD effect. In this study, the application of these methods to Bundang as of 1997 and alternatively redesigned Bundang showed that about 39.94 percent of the vehicle traffic reduction effects occurred. Furthermore, the pedestrian traffic ratio of alternatively redesigned Bundang increased to 2.39%.

A study on structure optimization to improve operating characteristic of Spoke Type Motor (Spoke Type Motor의 운전특성 향상을 위한 구조 최적화연구)

  • Han, Kwang-Kyu;Jang, Ki-Bong;Kim, Gyu-Tak
    • Proceedings of the KIEE Conference
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    • 2008.04c
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    • pp.35-37
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    • 2008
  • This paper deals with a design to improve operating characteristic of spoke type BLDC motor. The rotor and stator is redesigned as rounding teeth of stator and pole surface of rotor. Result, cogging torque is reduced greatly. The Finite Element Analysis(FEM) is used to characteristics. The Prototype has been manufacturing to confirmed the validity of the proposed technique.

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Sample Redesign of Household Survey conducted by Korea National Statistical Office (통계청 가구표본설계)

  • 윤연옥;이명호
    • Proceedings of the Korean Association for Survey Research Conference
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    • 2003.06a
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    • pp.69-84
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    • 2003
  • Main monthly household surveys conducted by National Statistical Office are economically active population survey and household expenditure survey. Samples of these two surveys are redesigned every 5 years based on Census. This paper is about sample redesign of household survey conducted in 2002 based on 2000 Census.

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The Design of A Code Generator for RISC Architecture (RISC 아키텍춰의 코드 생성기 설계)

  • 박종덕;임인칠
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.27 no.8
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    • pp.1221-1230
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    • 1990
  • This paper presents a code generation method and an effective handling algorithm of ingeger constant multiplication for RISC machines in compiler design. As RISC Architectures usually use faster and more simply formed instructions than CISC's and most RISC processors do not have an integer multiplication instruction, it is required an effective algorithm to process integer multiplication. For the proposed code generator, Portable C Compiler(PCC) is redesigned to be suitable for an RISC machine, and composed an addition chain is built up to allow fast execution of constant multiplication, a part of integer one whicch appears very frequency in code generation phase.

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Multi-mode noise reduction of using piezoelectric shunt damping smart panels (압전 션트를 이용한 패널의 다중 모드 소음 저감에 관한 연구)

  • Kim, Joon-Hyoung;Kim, Jaehwan
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2002.11a
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    • pp.327.2-327
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    • 2002
  • In this paper, the transmitted noise reduction of smart panels of which passive piezoelectric shunt damping is used, is experimentally studied. Shunt damping experiments are based on the measured electrical impedance model. A passive shunt circuit composed of inductor, and load resistor is devised to dissipate the maximum energy into the joule heat energy For multi mode shunt damping, the shunt circuit is redesigned by adding a blocking circuit. (omitted)

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