• 한국자원식물학회지
• /
• 제21권2호
• /
• pp.139-143
• /
• 2008

다섯 종류의 가지과 식물을 대상으로 elicitor에 의해 생합성 되는 capsidiol의 함량을 측정하고 생합성에 관련된 P450 효소의 활성과 관련 유전자 발현을 조사하였다. 고추, 피망 및 담배는 capsidiol의 생합성에 관련된 두개의 유전자인 cyclase와 P450 유전자가 세포내에 존재하였고, elicitor의 처리에 의하여 유전자의 발현이 유도되고 효소의 촉매가 이루어 졌다. 가지세포에는 두개의 유전자가 게놈상에 존재하지만 P450 유전자는 발현되나, cyclase 유전자의 발현은 나타나지 않았다. 감자에는 capsidiol의 생합성에 관련된 P450 및 cyclase 유전자 모두가 존재하지 않은 것으로 나타났다. 본 실험에 사용된 P450과 cyclase 유전자는 식물이 정상상태에 있을 때는 전혀 발현되지 않으나 elicitor에 의해 특이적으로 유도 되는 특징을 보였고, 식물체 조직이나 기관별로 다양한 발현 양상을 보였다.

• 환경생물
• /
• 제38권4호
• /
• pp.724-732
• /
• 2020

본 연구에서는 기 선발한 Enterobacter asburiae ObRS-5 균주를 1×10⁸ cfu mL^-1 농도로 고추에 관주 처리했을 때 Phytophthora capsici에 의한 고추역병을 74.6% 방제하는 효과가 있었다. E. asburiae ObRS-5 균주에 의한 고추역병 방제 메커니즘을 확인하기 위해 고추의 PR1, PR4 및 PR10 유전자를 특이적으로 증폭하는 프라이머를 이용하여 quantitative PCR을 수행하였다. 그 결과 E. asburiae ObRS-5 균주를 처리한 고추에서 대조구와 비교하여 상기 세 가지 유전자의 발현이 모두 높은 수준을 유지하였다. 또한 E. asburiae ObRS-5 균주는 고추의 생육을 억제하지 않으면서 ISR 반응을 유도하는 것으로 나타났다. 이러한 결과를 통하여 P. capsici이 침입할 때 E. asburiae ObRS-5 균주가 매개하는 ISR 메커니즘을 통해 Phytophthora 역병의 제어가 가능한 것으로 사료된다.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.95.1-95
• /
• 2003

Plant growth promoting rhizobacteria (PGPR) have been shown to suppress phytopthora blight. This suppression has been related to both microbial antagonism and induced resistance. The PGPR isolates were screened by dual culture plate method and most of the isolates were showed varying levels of antagonism. Among the PGPR isolates pyoverdin, pyochelin and salicylic acid producing strains showed the maximum inhibition of mycelial growth of Phytopkhora capsici and increased plant growth promotion in red pepper. PGPR isolates further analysed for its ability to induce production of defence related enzymes and chemicals. The activities such as Phenyle alanin ammonia Iyase (PAL), Peroxidase (PO), Polyphenol oxidase (PPO) and accumulation of phenolics were observed in PGPR pretreated red pepper plants challenged with Phytopkhora capsici. The present study shows that an addition of direct antagonism and plant growth promotion, induction of defense related enzymes involved to enhance resistance against invasion of P. capsici in red pepper.

• 식물병연구
• /
• 제10권3호
• /
• pp.203-208
• /
• 2004

붉은 고추 탄저병균의 우점종인 Colletotrichum gloeosporioides는 C. acutatum에 비해 병원성이 강하였다. C. gloeosporioides는 G와 R계통이 존재하였으며, G계통이 R계통보다 더욱 강한 병원성을 보였다. 붉은 고추 품종간의 병원성 검정 결과, 금세기 품종이 가장 감수성이었고, 파페트 품종이 가장 약한 감수성이었다. 참깨, 홍화, 마, 그리고 딸기와 같은 다른 기주에서 분리되어진 C. gloeosporioides는 붉은 고추에 감염을 일으켰다.

• Mycobiology
• /
• 제46권2호
• /
• pp.122-128
• /
• 2018

Arbuscular mycorrhizal fungi (AMF) are well-known for their ability to improve plant growth and help plants withstand abiotic stress conditions. Unlike other fungi and bacteria, AMF cannot be stored, as they are obligate biotrophs. Long-term preservation of AMF spores is challenging and may lead to the loss of viability and efficiency. This study aimed to understand the effect of prolonged subculture of AMF species on the growth and glomalin-related soil protein (GRSP) from red pepper (Capsicum annuum L.). AMF spores were mass-produced using different techniques and subcultured in pots with sorghum sudangrass as the host plant for 3 years. Experimental soil samples were collected from natural grassland. Five different AMF inocula were used in triplicate as treatments. After 70 days of growth, red pepper plants were harvested and plant dry weight, plant nutrient content, mycorrhizal colonization, AMF spore count, and soil glomalin content were determined. AMF-treated plants displayed higher dry weight than controls, with only fruit dry weight being significantly different. Similarly, significant differences in phosphorous and potassium contents of the above-ground plant parts were observed between mycorrhizal and control treatments. In addition, soil GRSP content was significantly higher in plants inoculated with Rhizophagus sp. and Gigaspora margarita. The increased plant growth and GRSP content suggest that AMF can be maintained for 3 years without losing their efficiency if subcultured regularly with different symbiotic host plants.

• 한국식품저장유통학회지
• /
• 제14권3호
• /
• pp.227-232
• /
• 2007

홍고추를 원료형태에 따라 원형, 세절, 분쇄로 제조하여 $-5^{\circ}C,\;-20^{\circ}C$ 및 $-40^{\circ}C$ 저장고에 보관하였다. 고추의 표면색도는 저장온도 조건에 따라 저장일수가 증가할수록 약간 감소하는 경향을 보였으며, 비타민 C 함량은 원형 홍고추가 세절고추나 분쇄고추보다 저장기간 동안 완만하게 감소하는 경향을 보였다. 저장온도별로는 $-40^{\circ}C$ 저장군에서 비타민 C 함량이 가장 높게 유지되었으며, $-5^{\circ}C$ 저장군에서 저장 2개월째 50% 이하로 급격히 감소하였다. 유리당 함량은 저장일수가 증가할수록 모든 처리구에서 저장 2개월까지 약간 증가하다가 점차적으로 감소하였으며 $-5^{\circ}C$ 저장온도 처리군에서의 감소변화가 $-20^{\circ}C,\;-40^{\circ}C$ 저장온도 처리군보다 심하게 나타났다. 냉동고추를 실온 해동시 드립로스의 양이 냉장 해동, microwave oven 해동시보다 약 2배 가까이 드립로스양이 발생하였으며, 표면색도는 고온 해동시 갈색화가 심하게 나타났다. 해동조건에 따른 냉동고추의 성분 변화는 냉장 해동과 microwave oven 해동시 가장 적게 나타났으며, 해동 시간을 고려한다면 microwave oven 해동이 가장 실용적인 방법이라고 여겨진다.

• 한국토양비료학회지
• /
• 제45권1호
• /
• pp.59-65
• /
• 2012

Plant growth-promoting effects of rhizobacterial inoculation obtained in pot experiments cannot always be dependably reproduced in fields. In this study, we investigated the effect of inoculation with Azospirillum brasilense and Methylobacterium oryzae, which have displayed growth promoting effects in several pot experiments, on growth and fruit yield of red pepper under field condition in a plastic-film house. Four rows spaced 90 cm apart were prepared after application of compost ($10Mg\;ha^{-1}$), and red pepper seedlings (Capsicum annum L., Nocgwang) were transplanted in each row with 40-cm space. Experimental treatments were consisted of A. brasilense CW903 inoculation, M. oryzae CBMB20 inoculation, and uninoculated control. Twelve plots, 10 plants per plot, were allotted to the three treatments with four replicates in a completely randomized design. At the time of transplanting, 50 mL of each inoculum ($1{\times}10^8cells\;mL^{-1}$) was introduced into root zone soil of each plant, and re-inoculated at 7 and 14 days after transplant. Plant growth and fruit yield were measured during the experiment. Both A. brasilense CW903 and M. oryzae CBMB20 could not promote growth of red pepper plants. All growth parameters measured were not significantly different among treatments. There were large variations in fruit yield recorded on plot basis, and no statistically significant differences were found among treatments. The failure to demonstrate the expected plant growth promoting effect of the inoculants is possibly due to various environmental factors, including weather and soil characteristics, reducing the possibility to express the potential of the inoculated bacterial strains.

• Journal of Plant Biology
• /
• 제39권3호
• /
• pp.167-172
• /
• 1996

Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

• 한국환경농학회지
• /
• 제37권1호
• /
• pp.9-14
• /
• 2018

BACKGROUND: The use of green manure and compost as organic fertilizer may increase crop yield and soil fertility due to improved soil nutrient availability and soil organic matter content (SOM). This study aimed to investigate the effects of hairy vetch (Vicia villosa L.) and compost application on red pepper growth, yield, fruit quality and soil health. METHODS AND RESULTS: The treatments were no fertilizer (CON), chemical fertilizer (CF), hairy vetch (HV), and livestock compost+HV (LC+HV). Red pepper seedlings (70 days old) were transplanted and maintained in experimental plots for 140 days. Plant dry weight, micro- and macronutrient contents of plants and soil chemical properties were determined. All fertilizer treatments significantly increased plant dry weight. Fruit yield was significantly highest with HV treatment. As for nutrient content, plants in HV and LC+HV treatments have significantly higher K and Ca contents than the other treatments. Regarding soil properties, HV and LC+HV application significantly altered the soil chemical properties. Significantly higher SOM was observed in HV and LC+HV treated soils. CONCLUSION: The results of this study suggest that short-term application of hairy vetch and compost is an effective alternative to the conventional chemical fertilizer to increase fruit yield red pepper and improve soil health.

• 한국균학회지
• /
• 제23권2호통권73호
• /
• pp.114-120
• /
• 1995

병든 고추에서 수집한 종자에서 Colletotrichum dematium, C. gloeosporioides 및 Glomerella cingulata 등이 습지법에 의해서 검출되었다. C. gloeosporioides는 공시된 종자시료에서 우점종이었으며 그 다음이 C. dematium, G. cingulata의 순이었다. 탄저병균의 종자내 감염부위를 조사한 결과, 종피, 배유 및 자엽에서 C. dematium, C. gloeosporioides 및 G. cingulata가 검출되었으며 3종의 탄저병균의 검출율은 종피에서 더 많았고 배유와 자엽에서는 적었다. C. dematium, C. gloeosporioides와 G. cingulata의 종자감염은 종자부패, 모잘록 및 모마름병을 일으켰다. 접종시험결과 C. gloeosporioides가 가장 병원성이 강하였다. C. dematium은 상처가 있을 때 약한 병원성을 나타냈으며 G. cingulata는 유상 또는 성숙과에 대해서 약한 병원성이 있었다. 탄저병에 대한 종자소독 결과 베노람 수화제와 지오람 수화제의 효과가 우수하였다.