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The Ecological Character and Sequence Similarity Analysis of 18S rDNA from the Two Species of Grateloupia (Grateloupia, Rhodophyta) in Korea (두 종의 한국산 홍조 지누아리류(Grateloupia filicina, Grateloupia divaricata)의 생태특성과 18S rDNA 염기서열 상동성 분석)

  • Kim, Young-Dae;Jin, Long-Guo;Song, Hong-In;Hong, Jung-Pyo;Lee, Chu;Jeon, Chang-Young;Kim, Su-Kyoung;Kim, Dong-Sam;Han, Hyoung-Kyun;Hong, Yong-Ki
    • Journal of Life Science
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    • v.16 no.5
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    • pp.723-728
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    • 2006
  • The species of Grateloupia filicina, Grateloupia divaricata in East Sea were investigated taxonmically in order to clarify taxonomic position. The ecological character, external morphology, anatomy of vegetative structure. Blade length are $15{\sim}40\;cm$, erect from discoidal holdfast of $3{\sim}10\;mm$ in diameter. Stipe $1{\sim}2.5\;cm$ long, narrowly cylindrical below, compressed above Grateloupia filicina. Main axis are long and compressed, $3{\sim}7\;mm$ broad in broadest part. Colors are scarlet to light red. Blade length are $10{\sim}25\;cm$, erect from discoidal holdfast of $3{\sim}8\;cm$ in diameter Grateloupia divaricata. Stipe are single and simple $2{\sim}5\;mm$ broad. Thallus composed of cortex and medulla in section ; cortex composed of $9{\sim}10$ layers of anticlinally arranged cortical cell, divided into outer, middle and inner parts. Partial fragments of nuclear 18S rDNAs from the two species of Grateloupia (Rhodophyta) were amplified using the PCR reaction and sequenced to compare their similarity. The partial sequences showed 98.9% similarity each other. Grateloupia filicina has 371 bp sizes and Grateloupia divaricata has 372 bp size. The G+C contents of Grateloupia filicina is 54.3% and Grateloupia divaricata is 53.64%.

Making Differential Diagnosis of Biliary Atresia Using Endoscopy (내시경을 이용한 영아 담즙울체 질환의 감별진단에 대한 연구)

  • Beck, Nam-Seon;Kang, I-Seok;Tchah, Hann
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.4 no.1
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    • pp.71-76
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    • 2001
  • Purpose: Many diagnostic modalities for neonatal cholestasis have shown features that are helpful, however until recently none of them are not pathognomonic of biliary atresia (BA). We carried out a prospective study of infants with cholestatic jaundice with the aim of establishing an efficient method of diagnosing BA. Methods: Twenty-seven consecutive infants with cholestatic jaundice were enrolled in this study and ranged from 7 to 152 days in age (mean; $51.2{\pm}34.2$ days). Gastroduodenoscopy was carried out using a fiberscope (Olympus N30). All the babies were fasted for at least 4 hours before the procedure and 20 ml of 10% dextrose solution was given at the time of endoscopy. The endoscopic examination focused on the 5 minutes observation of the evidence of biliary secretion. If there was lack of the evidence of the biliary secretion, endoscopy was removed and repeated the examination with some pause. Results: There are lack of the evidence of biliary secretion in all infants with BA. In non-BA group, 8 out of the 10 infants showed biliary secretion on the first trial, however one (Alagille syndrome) of the two infants without evidence of biliary secretion, finally exhibited biliary secretion on the second trial. The above observations resulted in the diagnostic accuracy of 96.3% with 100.0% sensitivity and 90.0% specificity. Conclusion: In light of the results from our relatively small study, endoscopy is a convenient, and relative inexpensive procedure. we strongly support the use of endoscopy for the diagnosis of BA in the screening and evaluation of infantile cholestasis.

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Effects of Presown Cold Stratification, $GA_3,\;KNO_3$ and Acetone Treatment on Germination of Crotalaria sessiflora L. (저온, $GA_3,\;KNO_3$ 및 Acetone 처리가 활나물 종자발아에 미치는 영향)

  • Kang, Jin-Ho;Kim, Yu-Jin;Jeon, Byong-Sam
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.2
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    • pp.124-129
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    • 2001
  • Crotalaria sessiflora is one of a few medicinal herbs among the legumes used as antitumor herb medicine but has lower seed germination and afterward seedling emergence. This study was carried out to elucidate the effects of cold stratification as presown treatment as well as $GA_3$, $KNO_3$ and acetone on its seed germination and seedling emergence. Cold stratification, $GA_3$, $KNO_3$ and acetone treatments were performed with different concentrations and period levels. Cold treatment was done under 14 hour red light illumination a day or darkness and the two others under darkness. On the basis of the best germination rate of each treatment, $GA_3$ and cold stratification treatments showed the highest germination rates at 0.1 mM but 12 hours and 2 week periods under darkness, respectively. $KNO_3$ and acetone treatments came out at 400 mM and 200 mM lasted for, respectively. The best germination rates from $KNO_3$ and acetone were comparatively higher than those from the former $GA_3$ and cold treatments. In evaluation of seedling emergence using the last two treatments, $KNO_3$ treated seeds were better performed than acetone treated seeds showed somewhat higher germination rate, implying that presown seed treatments must be evaluated by the combination with indoor and field tests.

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Marine Environments and Production of Laver Farm at Aphae-do Based on Water Quality and Phytoplankton Community (수질환경과 식물플랑크톤 군집 변화에 의한 압해도 김 양식장의 해양환경과 생산)

  • Yoon, Yang Ho
    • Korean Journal of Environmental Biology
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    • v.32 no.3
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    • pp.159-167
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    • 2014
  • In this study, I examined the water quality and phytoplankton community in aquaculture laver farm in the southwest part of Aphae-do, South Korea, based on the young leaf stage, middle leaf stage, and adult leaf stage of laver thallus from October, 2013 to January, 2014. It was observed that the Aphae laver farm, as located in shallow waters, was found to have a serious resuspsension of the surface sediments due to physical disturbance caused by winds and tidal mixing. Such a resuspension of surface sediments coupled with nutrients supply obstructs light penetration into the sea for its huge amount of total suspended matters. As a result for this reason, it was viewed toimpedthe growth of phytoplankton was impeded as it also competes with laver to absorb the same kinds of nutrients as laver does during the laver growth period in winter. Such elements of the marine environment in Aphae laver farm are in contrast with the environment of Japan, where nutrients including dissolved inorganic nitrogen, in particular, are insufficient to cause the recent laver bad harvest, discoloration and quality degradation while large diatoms, with their higher nutrients absorption efficiency than laver, generate winter red tide. In other words, an important factor to maintain the high laver production in the southern parts of West Sea of Korea was found to be the marine environment of its laver farms where large diatoms are prevented from growing due to nutrients supply and dense seston weights from resuspended matters by physical disturbances.

Cheonan Patrol Combat Corvette Sinking and Media Control: A Production Study on Investigative Programs (천안함 침몰 사건과 미디어 통제: 탐사보도 프로그램 생산자 연구)

  • Kim, Sang-Gyoon;Han, Hee Jeong
    • Korean journal of communication and information
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    • v.66
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    • pp.242-272
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    • 2014
  • This study examines why follow-up investigative programs about the sinking of the Cheonan Patrol Combat Corvette(ROKS Cheonan) have not been produced, despite much speculation surrounding the cause of the sinking since November 2010, when KBS investigative program, In-Depth 60 Minutes which had covered the incident was aired. We have uncovered four reasons through a series of in-depth interviews with producers and reporters, as follows. First, Korean military authorities monopolized relevant information, while the producers could not prove or confirm the validity of the findings of the JIG(Joint Civilian-Military Investigation Group)'s report because the facts had been revealed partially and selectively by the authorities and the press' scientific investigation were rejected by the authorities. Second, the crew of In-Depth 60 Minutes was subjected to severe disciplinary action by the Korea Communications Commission. This caused a chilling effect, - it broke the producers' resolve to further explore the reason behind the sinking. Furthermore, the screening of the investigative documentary, Project Cheonan Ship was cancelled without prior notice, presumably by the power of the State and markets. Third, the reorganization and shake-up of personnel by broadcasting stations' presidents appointed by the power of the State crushed the spirit of PD Journalism. Finally, the "red purge" factions stigmatized by the political or military, or the mainstream press, caused the producers or broadcasters to engage in self-censorship. The idea has been used in Korea as a smoke screen to deflect public attention. Nevertheless, the producers hope to shed light on the Cheonan sinking incident and to prove reasonable doubts by pursuing follow-up investigations.

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Effects of Electrical Stimulation on the Mast Cell of Skin in Rats (전기자극이 흰쥐의 피부 비만세포에 미치는 영향)

  • Lee Jae-Hyoung;Jekal Seung-Joo;Park Seung-Teack
    • The Journal of Korean Physical Therapy
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    • v.11 no.3
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    • pp.81-87
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    • 1999
  • The purpose of this study was to determine the effect of electrical stimulation on the number of MCs and percent of degranulated MCs in rat skin. Twelve male Sprague-Dawley rats were divided into two group; electrical stimulation group (n=6) and control group (n=6). Each animals hair on the back was removed. The electrical stimulation group received an positive rectangular pulsed electrical stimulation, while the control group was given the same treatment without electricity. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with toluidine blue-nuclear fast red and alcian blue-safranin O. respectively. The MCs were counted using a light microscope and computerized image analysis system and calculated as the density and the percent. A t-test showed a significantly higher density of MCs in the electrical stimulated rats than control rats(p<0.05), and the percent of degranulated MCs in the electrical stimulated rats was higher than in the control rats (p<0.05) in toluidine blue stained sections. The density of MCs was significantly higher in the electrical stimulated rats than the control rats in alcian blue-safranin O Stained sections (P<0.01). An analysis of variance showed that the densities of CTMCs was significantly lower than the densities of MMCs and mixed MCs in electrical stimulated rat in alcian blue-safranin O Stained sections (p<0.05). These results suggest that the electrical stimulation may have potential for increasing the number of MCs and lead to degranulate the MCs in rat skin.

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Prediction of weight loss of low temperature storage tomato (Tiwai 250) by non-destructive firmness measurement (비파괴적인 경도 측정을 통한 저온저장 토마토(티와이250)의 감모율 예측)

  • Cui, Jinshi;Yoo, Areum;Yang, Myongkyoon;Cho, Seong In
    • Food Science and Preservation
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    • v.24 no.2
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    • pp.181-186
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    • 2017
  • This study was conducted to investigate the weight loss, firmness, external color and vitamin C (VC) content of tomatoes (Lycopersicon esculentum) using non-destructive method to measure identical tomato samples during 15 days storage at low temperature and high humidity. Tomatoes were harvested at the light red stage, sorted, box packed and then stored in thermo-hygrostat ($10{\pm}1^{\circ}C$, $90{\pm}10%RH$). The quality changes in weight loss, firmness and external color were measured every 3 day interval. Weight loss was increased by $1.13{\pm}0.15%$, but it may not be considered to affect quality. Surface color of fruit was changed, especially in lightness and hue angle value. The color values were analyzed by analysis of variance (ANOVA), and the results were significant (p<0.001). Firmness of fruit declined during storage, but it did not decrease in direct proportion. On the storage of day 15, firmness was decreased to 40% of initial state. At last, all the experiment data are summarized and the relationship between firmness and weight loss is analyzed to construct a linear regression mathematical model that can predict the weight loss with the firmness value measured by non-destructive method. This research result could be useful in helping tomato exporters and suppliers to get real-time quality factor by using proposed method and regression model.

Color Filter Based on a Sub-wavelength Patterned Metal Grating (광파장 이하 주기를 갖는 금속 격자형 컬러필터)

  • Lee, Hong-Shik;Yoon, Yeo-Taek;Lee, Sang-Shin;Kim, Sang-Hoon;Lee, Ki-Dong
    • Korean Journal of Optics and Photonics
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    • v.18 no.6
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    • pp.383-388
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    • 2007
  • A color filter was demonstrated incorporating a patterned metal grating in a quartz substrate. The filter is created in a metal layer perforated with a symmetric two-dimensional array of circular holes, with the pitch smaller than the wavelength of the visible light. A finite-difference time-domain simulation was performed to analyze the device by investigating the effect of structural parameters like the grating height, the period, the hole size, and the refractive index of the hole-filling material on its performance. The device performance was especially optimized by controlling the refractive index of the material comprising the holes of the grating. And two different devices were fabricated by means of the e-beam direct writing with the following design parameters: the grating height of 50 nm, the two pitches of 340 nm for the red color and 260 nm for the green color. For the prepared device with the period of 340 nm, the center wavelength was 680 nm and the peak transmission 57%. And for the other device with the pitch of 260 nm, the center wavelength was 550 nm and the peak transmission was 50%. The filling of the hole with a material whose refractive index is matched to that of the substrate has led to an increase of ${\sim}15%$ in the transmission efficiency.

Influence of Copper Azole Retention Level to Wood Decay (목재 내 Copper Azole 보유량이 목재부후에 미치는 영향)

  • Lee, Hyun-Mi;Son, Dong-Won;Lee, Han-Sol;Hwang, Won-Joung
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.1
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    • pp.112-121
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    • 2015
  • In this study, Copper Azole (CuAz), a domestically available wood preservative for pressure treatment, was employed to perform an experimental research on its infiltration and decay properties in Japanese Red Pine. Test specimens were pressure-injected with CuAz-2 preservative to measure its preservative effectiveness, and then its impact on weight and mass losses. Furthermore, wood specimens were treated with CuAz-2 preservatives of various concentration levels before they were decayed with brown-rot-fungi in order to observe decay properties on light microscope (LM) and field emission scanning electron microscope (FE-SEM). As a result, untreated specimen by Fomitopsis palustris showed the mass loss of more than 40%, and the value of preservative effectiveness of CuAz-2 by indoor decay was $1,73-3.32kg/m^3$. The concentration levels of CuAz-2 preservative were shown to cause significant variations in terms of decay progresses in the cross section, radial section, and tangential section. By contrast, untreated specimens had underwent serious decays in early wood, late wood, longitudinal resin canals, and ray, which led to vertical destruction of wood texture. As for the radial section, ray tracheid, ray parenchyma cell, and window like pits were decayed and destroyed. In the case of tangential section, uniseriate rays and vertical resin canals were seriously decayed. In conclusion, this study indicates that the adequacy of the current CuAz injection amount should be reviewed in the domestic environment because there are significantly different decays at different decay conditions.

Change of Binocular Vision Induced by Longitudinal Chromatic Aberration during Near Work (근거리 작업 시 종색수차에 따른 양안시의 변화)

  • Kim, Se-il;Park, Mijung;Kim, So Ra
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.2
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    • pp.219-228
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    • 2015
  • Purpose: The current study was performed to compare the difference in binocular visual function depending on variable background colors at near work. Method: Fifty four adults (18 males, 34 females) who consented to the present study and had no ocular disease, ocular surgery history, strabismus and amblyopia with normal binocular vision were participated into this study. The subjects were asked to read the novels with black letter printed on white, red, green and blue background for 15 min. Then, their heterophoria, AC/A ratio, near point of convergence, accommodation facility, relative accommodation and vergence were measured before and after reading. The difference of measurements were compared. Result: Overall heterophoria was tended to decrease with regardless of background color. AC/A ratio showed a tendency of increase after reading the novels with all backgrounds except white background. Near point of convergence was significantly increased compared to before reading at all background color. Accommodative facility of dominant and non-dominant eyes were also significantly increased after reading however, binocular accommodative facility showed a tendency of decrease. Negative relative accommodation also decreased at all background colors however, the change of positive relative accommodation was not significantly different. In case of vergence, there was significant difference in break point of far BO and recovery point of far BI by the wavelength of background color. Conclusions: From the results, it was known there is convergence change depending on the wavelength of light even though same amount of accommodation and convergence is required when doing near work for certain period. Thus, it can be suggested that the adjustment of the near working environment which perception of various color was required, should be conducted according to the main wavelength.