• Title/Summary/Keyword: Recycling of wastes

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Biodegradation of marine microplastics by the whole-cell catalyst overexpressing recombinant PETase (PET분해효소(PETase) 과발현 전세포 촉매의 해양미세플라스틱 생분해 활성 연구)

  • Hyunji, Kim;Jong-Ha, Park;Ae-Ran, Park;Dae-Hee, Lee;Joonho, Jeon;Hyuk Taek, Kwon;Sung In, Lim
    • Journal of Marine Bioscience and Biotechnology
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    • v.14 no.2
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    • pp.133-142
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    • 2022
  • The increased production and consumption of polyethylene terephthalate (PET)-based products over the past several decades has resulted in the discharge of countless tons of PET waste into the marine environment. PET microparticles resulting from the physical erosion of general PET wastes end up in the ocean and pose a threat to the marine biosphere and human health, necessitating the development of new technologies for recycling and upcycling. Notably, enzyme-mediated PET degradation is an appealing option due to its eco-friendly and energy-saving characteristics. PETase, a PET-hydrolyzing enzyme originating from Ideonella sakaiensis, is one of the most thoroughly researched biological catalysts. However, the industrial application of PETase-mediated PET recycling is limited due to the low stability and poor reusability of the enzyme. Here we developed the whole-cell catalyst (WCC) in which functional PETase is attached to the outer membrane of Escherichia coli. Immunoassays are used to identify the surface-expressed PETase, and we demonstrated that the WCC degraded PET microparticles most efficiently at 30℃ and pH 9 without agitation. Furthermore, the WCC increased the PET-degrading activity in a concentration-dependent manner, surpassing the limited activity of soluble PETase above 100 nM. Finally, we demonstrated that the WCC could be recycled up to three times.

Biosorption of Copper Ions by Recycling of Castanea crenata (밤나무 재활용에 의한 구리 이온의 생물흡착)

  • Choi, Suk Soon
    • Applied Chemistry for Engineering
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    • v.25 no.3
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    • pp.307-311
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    • 2014
  • In this present study, Castanea crenata was found as an excellent biosorbent for the removal capability of copper ions among four different wood wastes (Castanea crenata, Pinus densiflora, Larix kaemoferi and Robinia pseudoacaia). Also, the removal efficiencies of 5, 10, 20, 40 and 50 mg/L copper ions using Castanea crenata from aqueous solution were investigated. The most effective particle size of Castanea crenata for removing 5 mg/L copper ions was found to be $43{\sim}63{\mu}m$. When the concentration of Castanea crenata increased, the removal efficiencies of copper ions were enhanced. In addition, when the 0.8 g/100 mL of Castanea crenata was used for 30 min, the removal efficiencies of 20 and 40 mg/L copper ions were 99% and 85%, respectively. Moreover, the chemical treatment of Castanea crenata with 1 M sodium acetate was required to improve the removal ability for 50 mg/L copper ions. Meanwhile, 1 M hydrochloric acid was selected as the optimal desorption agent with 93% desorption efficiency of copper ions for recycling of modified Castanea crenata. Therefore, these experimental results could be employed as economical and practical engineering data for the development of copper removal processes.

Preparation of Ag Fine Particles from Aqueous Silver Solution by Reduction Reaction (Ag 水溶液으로부터 還元反應에 의한 Ag 微粒子의 製造 硏究)

  • Lee, Hwa-Young;Jin, Seon-Ah;Han, Young-Ju
    • Resources Recycling
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    • v.14 no.1
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    • pp.26-32
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    • 2005
  • A study on the preparation of Ag fine particles was performed through a reduction reaction using ascorbic acids as a reductant, which is one of the indispensable processes for the recycling of silver-bearing wastes. Silver nitrate solution in the range of 10~120 mmole/l was used and Tamol NN8906 or PVP was also used as a dispersant in the preparation of Ag fine particles size analyze, SEM, and TEM to determine the particle size and morphology of them. As a result, the reduction reaction of silver ions with ascorbic acid reached equilibrium within 10 min. It was found that about 60% excess of ascorbic acid was required in order to reduce completely silver ions in the solution. The particle size distribution of Ag particles prepared through the reduction reaction showed typically biomodal or trimodal distribution. Especially, initial Ag concentration in the solution, the type and amount of dispersant added during the reduction reaction played an important role in determining the mean particle size of Ag particles.

The Study of the Separation Efficiency of Wind Power Selector Using Computational Fluid Dynamics (전산유체역학을 이용한 풍력 선별기의 선별효율 연구)

  • Lee, Keon Joo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.21 no.3
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    • pp.74-81
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    • 2013
  • In this study, the separation efficiency of wind power selector (the direction of the air flow of the air to perform gravity separation method) of municipal solid waste which was landfilled was investigated to reduce amount of waste that is designed to increase the recycling rate of wastes for the ANSYS CFX Program's numerical methods with wind through the separator. When a suction device designed to suction 1000mL of a plastic bag, the separation efficiency was 100% and when the wind speed was 0.9 m /sec or more and when the wind speed was 1.6 m / sec or more, the efficiency of plastic bottles in a mixture of 500mL and 1500mL plastic bottle waste was 100% and the aluminum screening efficiency of 250mL aluminum can was 100 % when the wind speed was 2.3 m / sec. In the last, 5mm thick compressed aluminum can efficiency was 90 % when the wind speed was 2.4 m / sec.

Synthesis of $LiCoO_{2}$ Nanoparticles From Leach Liquor of Lithium Ion Battery Wastes by Flame Spray Pyrolysis

  • Lee Churl Kyoung;Chang Hankwon;Jang Hee Dong;Sohn Jeong-Soo
    • Resources Recycling
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    • v.14 no.6 s.68
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    • pp.37-43
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    • 2005
  • [ $LiCoO_{2}$ ] nanoparticles were synthesized from leach liquor of lithium ion battery waste using flame spray pyrolysis. Electrode Materials containing lithium and cobalt could be concentrated with thermal and mechanical treatment. After dissolution of used cathode materials of the lithium battery with nitric acid, the molar ratio of Li/Co in the leach liquor was adjusted at 1.0 by adding a fresh $LiNO_{3}$ solution. The nanoparticles synthesized by the flame spray pyrolysis showed clear crystallinity and were nearly spherical, and their average primary particle diameters ranged from 11 to 35 nm. The average particle diameter increased with an increase in the molar concentration of the precursor. Raising the maximum flame temperature by controlling the gas flow rates also led to an increase in the average diameter of the particles. The $LiCoO_{2}$ powder was proved to have good characteristics as cathode active materials in charge/discharge capacity and cyclic performance.

Review and Suggestion for Waste Transfer Stations in Seoul (서울시 생활폐기물 적환장 운영실태 분석 및 제언)

  • Yoo, Kee-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.24 no.4
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    • pp.21-29
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    • 2016
  • This study was conducted to review Waste Transfer Stations(WTSs) in Seoul, especially focusing on three aspects, main roles, neighbor friendly and regulation compliance, and to draw out recommendation for improvement. The 46 units of all 65 WTSs treated large amount of waste on a day shipping-out schedule, the other 19 WTSs treated small amounts of wastes which were transported after 3~30 days stock. About 57% of WTSs are located in residential or commercial area. Other 57% of WTSs were open without walls. 79% of WTSs were placed alone. Just 17% of WTSs were permitted as environmental infrastructures by urban planning law, and 31% of WTSs were operated by private waste haulers who were permitted by waste management law. Most WTSs in Seoul seemed to go well with on-going policy demand, such as regionalizing waste treatment facilities, expanding waste recycling, and so on. However lots of WTSs did not implement sufficient environmental protection measures, and partly not compliant with regulations related to urban infrastructures. In the future, the issue on sealing facilities and integrating functions of waste management facilities could be considered in order to fit in urban environment.

Preparation of Ag Nano-Powder from Aqueous Silver Solution through Reductive Precipitation Method (환원침전법을 이용한 수용액으로부터 은 나노분말의 제조 연구)

  • Lee Hwa-Yaung;Oh Jong-Kee
    • Resources Recycling
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    • v.14 no.6 s.68
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    • pp.21-27
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    • 2005
  • As one of the hydrometallurgical processes available in the recycling of silver-bearing wastes, the preparation of Ag nano-powder was investigated by a reductive precipitation reaction in silver solution using sodium formaldehydesulfoxylate and ascorbic acid as a reducing agent. Silver solution was prepared by dissolving silver nitrate with distilled water, and Tamol NN8906, PVP, SDS and caprylic acid were also used respectively as the dispersant to avoid the agglomeration of particles during the reductive reaction. Ag particles obtained from the reduction reaction from silver solution were characterized using the particle size analyzer and TEM to determine the particle size distribution and morphology. It was found that about $40\%$ excess of sodium formaldehydesulfoxylate was required to reduce completely silver ions in the solution. It alto appeared that the particle size generated with sodium formaldehydesulfoxylate was much greater than that with ascorbic acid. As far as the effect of dispersant on the Ag particles was concerned, the particle size distribution showed typically bimodal distribution in case of Tamol/FVP while very broad distribution ranged from 0.01 to $100{\mu}m$ appeared in case of SDS/caprylic acid.

From food wastes into useful probiotics: Development of Lactic acid bacteria as useful probiotics for animal feed without antibiotics using food waste (음식물류폐기물의 자원화를 위한 항세균 활성 유산균의 개발)

  • Seo, Jeong-Yong;Song, In-Geun;Lee, Dae-Gyu;Lee, Ki-Young;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.2
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    • pp.112-120
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    • 2006
  • For the conversion of food waste into a good recycling material such as animal feed without antibiotics, thirteen lactic acid bacteria, which can be used as good probiotics for animal feed, were isolated from the intestine and feces in pigs. All isolates showed strong tolerance to high salt (4% of NaCl), acid(pH4.0), and bile juice (0.8% of oxgall). The growth rate was best at $37^{\circ}C$ in all strains. Among the isolates, Lactobacillus plantarum CJY-22, L. brevis CJY-42, L. arizonensis CJY-3, and Pediococcus sp. CJY-41 showed higher and broader spectrum of antimicrobial activities against six different pathogens such as Salmonella, typhimurium. L. plantarum CJY-22 has also grown well at $25^{\circ}C$, making this strain as an appropriate candidate for the fermentation of food waste at room temperature, thus conducting the fermentation process cost-effectively.

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Current Status and future of Vermicomposting Industry in Korea (지렁이 퇴비화 운영사례 및 개선방향)

  • Kim, Jong-Oh;Lee, Chang-Ho;Choi, Hun-Gun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.4
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    • pp.89-98
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    • 2001
  • Present vermicomposting technology needs the broad land. Because of the high land cost, the land saving technology such as multistage must be needed. And present operation practices are mostly based on human power, mechanization and automation is needed. Also, present control practices based on individual experience need the scientific base control system. Even though the rough estimation of organic waste treatment showed that vermicompostiong could obtain more benefits than costs. But, the estimation is based on an ideal case without considering the technical and market. Generally, vermicomposting is considered as the more expensive technology than composting. Therefore, the practical proof the economic factor would be the most important to the market increase. Vermicomposting as the recycling technology has better condition than incineration and landfill. Propagation and advocating focused on its environmentally sound aspects would be needed continuously. Especially, demonstration and distribution of household vermicomposting would be a good approach. Vermicomposting facilities area was becoming larger, and earthworm feeding materials were also expanding to various organic sludges. These trends showed that vermicomposting is being enlightened to treat and recycle the organic wastes.

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Development of Triboelectrostatic Separation Technique for Material Separation of EVA & PET Mixture Plastic Wastes (EVA와 PET 혼합(混合) 폐플라스틱의 재질분리(材質分離)를 위한 마찰하전형(摩擦荷電形) 정전선별(靜電選別) 기술개발(技術開發))

  • Jeon, Ho-Seok;Park, Chul-Hyun;Baek, Sang-Ho;Kim, Byoung-Gon;Kim, Hyung-Seok
    • Resources Recycling
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    • v.18 no.1
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    • pp.13-21
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    • 2009
  • A research on material separation of EVA and PET mixture plastic waste using a triboelectrostatic separator has been carried out. It was found that PP was the best charging material to give the highest charge on the surface of EVA and PET mixture plastics with an opposite polarity. Therefore, a charger of pipe line type using PP material was manufactured for separation of EVA and PET mixture plastic waste. At optimum test conditions that used PP cyclone charger developed in this study, we could separate out PET with a glade of 98.7% and a recovery of 89.7%.