• Childhood Kidney Diseases
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• 제12권1호
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• pp.23-29
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• 2008

Kidney allograft transplantation is the most effective method of renal replacement for end stage renal disease patients. Still, it is another kind of 'disease', requiring immunosuppression to keep the allograft from rejection(allograft immune reaction). Immune system of the allograft recipient recognizes the graft as a 'pathogen (foreign or danger)', and the allograft-recognizing commanderin-chief of adaptive immune system, T cell, recruits all the components of immune system for attacking the graft. Proper activation and proliferation of T cell require signals from recognizing proper epitope(processed antigen by antigen presenting cell) via T cell receptor, costimulatory stimuli, and cytokines(IL-2). Thus, most of the immunosuppressive agents suppress the process of T cell activation and proliferation.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2003년도 춘계학술연구발표회
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• pp.16-16
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• 2003

Mismatches in a DNA duplex are mainly due to DNA duplication errors that are generated by improper function of DNA polymerase. MutS, MutL and MutH are crucial proteins for the initiation of the methyl-directed mismatch repairing in bacteria. MutS has an ATPase activity md recognize the mismatched or unpaired bases on DNA. After binding to a mismatch, MutS recruits MutL to mediate the activation of MutH an endonuclease, which cleaves the 5' site of d(GATC) on the un-methylated strand. Both MutL and MutS also have essential roles in the subsequent removal and re-synthesis of the daughter strand. We have determined the crystal structures of either intact or active fragments of each of these proteins, both alone and complexed with ligands (DNA, ADP and ATP). The biochemical and mutagenesis studies based on the detailed 3-D structures led to new insights into the role of the ATPase activity of MutS in the mismatch recognition and directions for future investigation of mismatch repair.

• 생명과학회지
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• 제17권11호
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• pp.1587-1592
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• 2007

Locus control region (LCR) is a cia-acting element which regulates the transcription of genes in developmental stage and/or tissue-specific pattern. Typically, LCR consists of several DNase I hypersensitive sites (HSs), where the binding motifs for transcriptional activators are present. The binding of activators to the HSs recruits chromatin modifying complexes to the LCR, opening chromatin structure and modifying histones covalently through the locus. LCR forms close physical contact with target gene located at a distance by looping away intervening region. In addition, non-coding RNA is transcribed from LCR toward target genes in continuously acetylated active domain. These structural and functional features of LCR suggest that the LCR plays many roles in chromatin activation and transcriptional regulation.

• IMMUNE NETWORK
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• 제16권3호
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• pp.147-158
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• 2016

The liver lies at the intersection of multiple metabolic pathways and consequently plays a central role in lipid metabolism. Pathological disturbances in hepatic lipid metabolism are characteristic of chronic metabolic diseases, such as obesity-mediated insulin resistance, which can result in nonalcoholic fatty liver disease (NAFLD). Tissue damage induced in NAFLD activates and recruits liver-resident and non-resident immune cells, resulting in nonalcoholic steatohepatitis (NASH). Importantly, NASH is associated with an increased risk of significant clinical sequelae such as cirrhosis, cardiovascular diseases, and malignancies. In this review, we describe the immunopathogenesis of NASH by defining the known functions of immune cells in the progression and resolution of disease.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1808-1816
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• 2016

As a scaffolding subunit of the PIK3C3/VPS34 complex, Beclin 1 recruits a variety of proteins to class III phosphatidylinositol-3-kinase (VPS34), resulting in the formation of a distinct PIK3C3/VPS34 complex with a specific function. Therefore, the investigation of a number of Beclin 1 domains required for the protein-protein interactions will provide important clues to understand the PIK3C3/VPS34 complex, of which Beclin1-VPS34 interaction is the core unit. In the present study, we have designed a bacterial overexpression system for the Beclin 1 domain corresponding to VPS34 binding (Vps34-BD) and set up the denaturing purification protocol due to the massive aggregation of Vps34-BD in Escherichia coli. The expression and purification conditions determined in this study successfully provided soluble and functional Vps34-BD.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2010년도 추계학술발표논문집 2부
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• pp.996-999
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• 2010

본 연구는 성인 남성들의 비만도에 영향을 주는 요인을 사회 경제적, 건강관련 행동적 측면에서 규명하기 위하여 일개광역시 A부대에 2009년 4월과 5월에 입소한 장병 4,923명을 대상으로 건강관련 행태관련 설문조사를 한 후 비만도를 산출하고 비만과 관련 요인을 분석하였다. 입대전 가구의 월평균 소득이 비만과의 관련성에서 긍정적 효과를 주는 것으로 판단된다. 그러나 우리나라의 경우 아직까지 경제적 수준에 따른 비만의 유병률에 대한 일관성 있는 결과가 도출되고 있지 못한 실정으로 연령, 유전, 직업, 교육수준, 소득 등의 지표들을 사용한 비만과의 관련성에 대한 구체적인 후속 연구가 필요할 것으로 생각된다.

• 해양환경안전학회지
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• 제25권6호
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• pp.749-756
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• 2019

본 연구는 신임해양경찰공무원의 교육훈련에 공공봉사동기가 영향을 미치는가를 검증하였다. 연구결과는 공공봉사동기의 하위변인인 자기희생, 공익몰입, 공공정책의 호감도는 교육만족도에 영향을 미치는 것으로 조사되었으나 공공봉사동기 하위변인인 동정심은 교육내용만족에 대한 영향요인은 기각되었다. 본 연구는 내재적 요인인 공직에 필요한 봉사정신, 희생정신과 같은 무형의 자산이 교육만족도영향요인임을 검증하였다는데 의미가 있다. 해양경찰은 육상경찰이나 다른 공직분야와 달리 해상이라는 장소적인 위험성으로 인하여 공직 가치의 중요성이 보다 중요시되므로 이를 고양하기 위한 다양한 제도와 연구의 필요성이 인정된다.

• 한국환경보건학회지
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• 제40권4호
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• pp.265-278
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• 2014

Microbes such as bacteria, fungi, archaea, protists, and viruses are ubiquitous and people are exposed to them continuously. Endotoxin is a component of the outer membrane of Gram-negative bacteria and a potent proinflammaotry substance. When a person is exposed to environmental endotoxin, an innate immune response is initiated upon the initial recognition and this response produces various inflammatory mediators and recruits inflammatory cells to the exposed tissues. A purified chemical form of endotoxin is called lipopolysaccharide (LPS), and the lipid A portion of the molecule is a biologically active moiety. Exposure to endotoxin may result in various complex health effects depending on time, route, and dose of exposure, as well as host susceptibility. Gene-environment interactions play important roles in health effects of endotoxin exposure, e.g. development or aggravation of asthma. To accurately assess exposure to endotoxin in environmental or epidemiologic studies, methods of sampling, extraction, and analysis must be carefully selected since the selected methods may substantially affect analytical results and there is no internationally-agreed standard method to date. The lack of a standardized method hampers the establishment of exposure-response relationships. While an internationally-agreed health-based exposure limit does not exist, the Dutch Expert Committee on Occupational Safety recently recommended $90EU/m^3$ as a health-based occupational exposure limit. The current article reviews various scientific issues on how we measure environmental endotoxin and the health effects of endotoxin exposure.

• The Journal of Korean Physical Therapy
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• 제23권5호
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• pp.1-5
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• 2011

Purpose: This study was designed to investigate the changes of postural sway and muscle activation while standing upright and performing a dual task. Methods: Nine healthy adults were recruited and provided their written informed consent. They performed a balance task with and without a cognitive task on a force flatform (Good balance, Metitur Ltd., Filand). Postural sway was measured as medio-lateral and anterior-posterior distance and the velocity of the center of pressure and muscle activations of the ankle dorsi- and plantar-muscle was measured. The recruits completed three trials and the data was analyzed by a paired t-test. Results: There were significant differences in the medio-lateral and anterior-posterior distance and the velocity of the center of pressure, and significant increases in the muscle activities of the tibialis anterior. Conclusion: These findings revealed that performing a dual task increases postural sway and muscle activation of the ankle when simultaneously maintaining balance and performing another cognitive task. Future studies should focus on balance training with a dual task for patients.

• 설비공학논문집
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• 제26권1호
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• pp.15-20
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• 2014

In this study, improvement of university education is suggested so that university graduates in Architectural Engineering can work in the plant engineering industry without major retraining, which is generally required by plant engineering companies. Before the disposition of manpower to a plant engineering site, new recruits are retrained for about 2~3 years, since university education is neither sufficient, nor appropriate to handle plant engineering tasks. It is necessary to implement practical plant engineering into university education, so that graduates can work effectively in plant engineering fields, without major retraining. For a case study of an S2 University located in Seoul, it is enough to supplement the interdisciplinary program with plant engineering subjects, if proper texts are developed. To replace the plant engineering education offered by the company with university education, the following measures should be taken. First, basic plant engineering should be taught for 15 hours. Second, education on design and drawings should be reinforced.