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Expression of the HSV-1 (F) Glycoprotein B Gene in Insect Cells Infected by HcNPV Recombinant

  • Cha, Soung-Chul;Kang, Hyun;Lee, Sook-Yeon;Park, Gap-Ju;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.10 no.3
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    • pp.355-362
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    • 2000
  • The Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene in the pHLA-21 plasmid was inserted into a baculovirus (Hyphantria cunea nuclear polyhedrosis virus) expression vector (lacZ-HcNPV) to construct a recombinant virus gB-HcNPV expressing gB. Spodoptera frugiperda cells infected with this recombinant virus synthesized and processed gB of approximately 120 kDa, which cross-reacted with the monoclonal antibody to gB. The recombinant gB was identified on the membrane of the insect cells using an immunofluorescence assay. Antibodies to this recombinant raised in mice recognize the viral gB and neutralized the infectivity of the HSV-1 in vitro. These results show that the gB gene has the potential to be expressed in insect cells. They also demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the lacZ-HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

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Estimation of Coastal Terrain Differences by time-series using GSIS and The chart (GSIS를 이용한 해안 지형의 시계열 변화량 추출)

  • 양인태;한성만;최승필
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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    • 2002.04a
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    • pp.219-228
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    • 2002
  • Because western sea on the shore development is threatening sea ecosystem by decrease of silt and large size land-reclamation work by industrialization causes surrounding weather change and sea change, generate much changes bottom of the sea topography and coastline. Also, is influencing to route for safe entry into port of ship, departure. Therefore, this research did 0m boundary line, anxiety 2m line which appear to coastline of land portion and the border of silt using sea base level of lowest low tone side that is base line that appear because of sea waves to basis data numerical value Tuesday, numerical value by divide drawing that is changed to 4 area and analyze change degree of new airport construction and new town development, seashore by western sea district along the coast development of tide embankment construction and so on and bottom of the sea recognize.

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OPTIMUM ALLOCATION OF PORT LABOR GANGS IN CASE OF MULTIPLE SHIPS (항만하역노동력의 최적배분에 관한 연구 (II) 선박군의 경우)

  • 이철영;우병구
    • Journal of the Korean Institute of Navigation
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    • v.13 no.3
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    • pp.37-44
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    • 1989
  • Recently recognize the labor productivity of port physical distribution system in the port and shipping areas, Much Efforts for evaluating this productivity has been made continuously. BUt still there is little study, so far, on a systematic research for the management of port labor gangs, and even those were mainly depended on a rule of thumb. Especially the object of this study is to introduce the method of optimal allocation and assignment for the labor gangs per pier unit in the multiple ships berthed at an arbitary pier or port. In case the multiple ships have a homogeneous cargoes or do not have sufficient labor gangs to be assigned. The problem of optimal allocation and assignment of the labor gangs to be i) formalized with multi-state decision process in form of difference equation as the pattern which converted the independent multiple ships into a single ship with the intra-multiple ships, and ii) the optimal size of labor gangs could be obtained through the simple mathematical method instead of complicated dynamic programming, and iii) In case of shortage of labor gangs available the evaluation function considering the labor gangs available and total shift times was introduced, and iv) the optimal allocation and assignment of labor gangs was dealt at the point of minimizing the summation of the total shift times and at the point of minimizing the total cost charged for the extra waiting time except PHI time during port times for the multiple ships combinations.

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A Study on Sonia Delaunay's Painting, Fashion and Fabric Design (쏘냐 들로우네의 회화와 의상$\cdot$직물디자인 세계)

  • Yim Sun-Hi
    • Journal of the Korean Society of Clothing and Textiles
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    • v.10 no.1
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    • pp.85-95
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    • 1986
  • Sonia Delaunay(1885$\~$1979) was one of great pioneers of abstract art, who looked at clothes and fabrics with a plastics eyes. In Association with her husband Robert Delaunay, they were instrumental in founding the movement of Orphism, she proceeded to mix strong and bright colors into her art and had a brilliant influence on the decoration and women's fashion of the 1920's. Having a strong sense for dramatic and decorative color derived in part from childhood remembrances of Russian folk art she initiated a total revolution in which she created her first simultaneous dress with multi-colored samples of materials. She extended the principle of color's simultaneity to the field of fashion, fabric design and applied art. She was interested in the dress for ballet and opera. Fashion designer Paul Poiret asked her to design the fabrics and she created the geometric and abstract patterns with her strong color. It seemed that her design was revolutionary and avant-garde. Always she desired not only art of seeing but also art of living. The purpose of this study is to recognize the influence she had upon the history of modern fashion and fabric design. It was remarked that her paintings served as a basis for later developments in Kinetics Art and had influences on 1980' s abstract patterns for silk dress. Finally, the concept 'simultaneity' of her art signifies endless rhythmes in space and time.

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A Study on Global Compliance of Global Companies under the Circumstance of Export Control (전략물자 무역환경에 따른 글로벌기업의 글로벌 컴플라이언스에 관한 연구)

  • Choi, Choon-Ho
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.39
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    • pp.367-389
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    • 2008
  • On the stage of an international trade with well-developed transportation, communication system and proliferation of free trade, global companies who are eager to sustain business growth by cutting cost and pioneering new market are facing a new challenge named "Global Compliance" of business transparency, export and import regulations, and potential international business environment. The purpose of a global compliance is to monitor and regulate a company's trade activities to reduce the risk of transactions that might violate relevant countries' laws, regulations, or standards. After the 911, for strengthening the non-proliferation of the export control goods, UNSCR1540(United Nations Security Council Resolution 1540(2004)) was declared in order to enforce the members to adopt the rules in the resolution into their national laws and regulations. Companies does not realized that they need to get rid of the risks because they underestimate the importance of international security, caused by their careless management. That is why currently the export control program is not fully observed by the most. Lack of awareness for the export control and the poor system of each members could be the reason for this unstable operating status. With this background, this thesis will study on the meaning of export control, schemes for companies to recognize its importance and governmental guideline to support global companies.

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An Adaptation System based on Personalized Web Content Items for Mobile Devices

  • Kim, Su-Do;Park, Man-Gon
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.3 no.6
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    • pp.628-646
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    • 2009
  • Users want to browse and search various web contents with mobile devices which can be used anywhere and anytime without limitations, in the same manner as desktop. But mobile devices have limited resources compared to desktop in terms of computing performance, network bandwidth, screen size for full browsing, and etc, so there are many difficulties in providing support for mobile devices to fully use desktop-based web contents. Recently, mobile network bandwidth has been greatly improved, however, since mobile devices cannot provide the same environment as desktop, users still feel inconvenienced. To provide web contents optimized for each user device, there have been studies about analyzing code to extract blocks for adaptation to a mobile environment. But since web contents are divided into several items such as menu, login, news, shopping, etc, if the block dividing basis is limited only to code or segment size, it will be difficult for users to recognize and find the items they need. Also it is necessary to resolve interface issues, which are the biggest inconvenience for users browsing in a mobile environment. In this paper, we suggest a personalized adaptation system that extracts item blocks from desktop-based web contents based on user interests, layers them, and adapts them for users so they can see preferred contents first.

A Study on Apartment Residents' Conciousness of Evacuation Safety by Residential Height (공동주택 거주자의 거주 층수에 따른 피난 안전 의식에 관한 연구)

  • Heo, Jeong-Eun;Na, Wook-Jung;Jeon, Gyu-Yeob;Hong, Won-Hwa
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2006.11a
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    • pp.310-315
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    • 2006
  • Recently, an apartment has been changed to be high and diverse. In addition, it has been popularized rapidly. The more number of residents are increased, the more danger of the fire is also increased. If residents are in the upper floor when a fire breaks out, they have much longer evacuation path than the usual. What is more, their condition becomes more unstable. so, riskiness of evacuation is very serious. We should set up environment of evacuation safely. Even though some evacuation system is set up , the dangerous situation is more likely to be occurred. Because it depend on the person. If the person in the building don't recognize that system, it's valueless. Therefore it's very important to understand residents' conciousness level which is related to flee for safety in the fire. In this study, we examine into residents' aware safety level by their residential type. And according to this data, let residents know how they escape from danger . As a result, they can get out of danger when a fire breaks out.

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Advisable Direction of Sensitivity-Quality Factors on Railroad Sector (철도분야의 감성품질 요소 정립방향)

  • Song, Min-Ho;Park, Jae-Hyun;Kwon, Sun-Bak;Song, Yong-Su
    • Proceedings of the KSR Conference
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    • 2011.10a
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    • pp.2250-2255
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    • 2011
  • There has been a growing interest toward railroad conjunction with opening of high-speed rail in 2004 and concurrent implementation of nation's green growth policies. Meanwhile, users' expectation on service of public transportation, especially railroad has been growing than since ever due to heightened quality of life, entering aging society, voice of equity for minorities, and greater interest in environments of communities. In order to come up with the expectations, actual improvements for user's convenience/comfort and its assessment strategies in terms of service quality level are necessary. This study defines the index of this aspect of service level as 'Sensitivity Quality'. This is conceived as one of the important factors to define travel cost as do the travel time and fare, but hardly taken into consideration when assessing a transportation system or policy-making for a transportation project. The elements included for the assessment of the sensitivity quality in this study are feeling of a ride, conformability and convenience what users feel and recognize using a train. This paper suggests that establishing sensitivity quality elements would contribute in foundation of assessing service quality and provide improved public transportation services through their scientific and objective development and management.

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Optical Music Score Recognition System for Smart Mobile Devices

  • Han, SeJin;Lee, GueeSang
    • International Journal of Contents
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    • v.10 no.4
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    • pp.63-68
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    • 2014
  • In this paper, we propose a smart system that can optically recognize a music score within a document and can play the music after recognition. Many historic handwritten documents have now been digitalized. Converting images of a music score within documents into digital files is particularly difficult and requires considerable resources because a music score consists of a 2D structure with both staff lines and symbols. The proposed system takes an input image using a mobile device equipped with a camera module, and the image is optimized via preprocessing. Binarization, music sheet correction, staff line recognition, vertical line detection, note recognition, and symbol recognition processing are then applied, and a music file is generated in an XML format. The Music XML file is recorded as digital information, and based on that file, we can modify the result, logically correct errors, and finally generate a MIDI file. Our system reduces misrecognition, and a wider range of music score can be recognized because we have implemented distortion correction and vertical line detection. We show that the proposed method is practical, and that is has potential for wide application through an experiment with a variety of music scores.

Improved MOG Algorithm for Periodic Background (주기성 배경을 위한 개선된 MOG 알고리즘)

  • Jeong, Yong-Seok;Oh, Jeong-Su
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.10
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    • pp.2419-2424
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    • 2013
  • In a conventional MOG algorithm, a small threshold for background decision causes the background recognition delay in a periodic background and a large threshold makes it recognize passing objects as background in a stationary background. This paper proposes the improved MOG algorithm using adaptive threshold. The proposed algorithm estimates changes of weight in the dominant model of the MOG algorithm both in the short and long terms, classifies backgrounds into the stationary and periodic ones, and assigns proper thresholds to them. The simulation results show that the proposed algorithm decreases the maximum number of frame in background recognition delay from 137 to 4 in the periodic background keeping the equal performance with the conventional algorithm in the stationary background.