• KSBB Journal
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• v.17 no.1
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• pp.20-25
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• 2002

We performed a basic experiment for rapid, on-line, real-time measurement of HBsAg by using a surface plasmon resonance biosensor to quantify the recognition and interaction of biomolecules. We immobilized the anti-HBsAg polyclonal antibody to the dextran layer on a CM5 chip surface which was pre-activated by N-hydroxysuccinimide for amine coupling. The binding of the HBsAg to the immobilized antibody was measured by the mass increase detected by the change in the SPR signal. The binding characteristics between HBsAg and its antibody followed typical monolayer adsorption isotherm. When the entire immobilized antibody was interacted, there was no additional, non-specific binding observed, which suggested the biointeraction was very specific as expected and independent of the ligand density. No significant steric hindrance was observed at 17.6 nm/$mm^2$ immobilization density. The relationship between the HBsAg concentration in the sample solution and the antigen bound to the chip surface was linear up to ca. $40\mu\textrm{g}$/mL, which is much wider than that of the ELISA method. It appeared the antigen-antibody binding was increased as the immobilized ligand density increased, but verification is warranted. This study showed the potential of this biosensor-based method as a rapid, simple, multi-sample, on-line assay. Once properly validated, it can serve as a more powerful method for HBsAg quantification replacing the current ELISA method.

• Management & Information Systems Review
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• v.15
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• pp.19-35
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• 2004

Top management's will was considered most positive by all of the surveyed local businesses, followed by organizational culture, information technology, evaluation and reward, and organizational structure in order. When influences of educational background on factors of success in knowledge management were analyzed, it was found that such background was affecting organizational structure at the significance level of 0.01 and information technology and top management's will at that of 0.05. Those who were more educated responded that success in knowledge management were dependent more on top management's will. but less on organizational structure. Concerning effects of organizational position on factors of success in knowledge management, it was found that such position was influencing top management's will at the significance level of 0.01 and organizational structure at that of 0.05. Those who were higher in organizational position responded that success in knowledge management were dependent less on top management's will, but more on organizational culture, and evaluation and reward. It as generally agreed among the surveyed businesses that knowledge management is a key strategy for business survival in the 21st century. To utilize knowledge management as their strategy, Korea's businesses should change their recognition of such management, reform their organizational culture, have CEOs of strong conviction and will and firmly establish their own information technology. It is necessary to make recognized the importance of knowledge management and culture knowledge management culture. If a person has a strong conviction or will of achieving goals by using intellectual efforts, creative imagination and necessary information, such information can be regarded as a proper knowledge. In conclusion, knowledge enterprises should strengthen their ability of knowledge use by associating their competition strategies with knowledge management. They also need to positively invest in the capture and new development of knowledge and build up infrastructures for knowledge management.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.277-282
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• 2011

We carried out shape recognition. We found out cucumber's feature shape by means of neural network and back propagation algorithm. We developed an algorithm which finds object position and shape in real image and we gained following conclusion as a result. It was processed for feature shape extraction of cucumber to detect automatic. The output pattern rates of the miss-detected objects was 0.1~4.2% in the output pattern which was recognized as cucumber. We were gained output pattern according to image resolution $445{\times}363$, $501{\times}391$, $450{\times}271$, $297{\times}421$. It was appeared that no change was detected. When learning pattern was increased to 25, miss-detection ratio was 16.02%, and when learning pattern had 2 pattern, it didn't detect 8 cucumber in 40 images.

• BMB Reports
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• v.40 no.1
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• pp.119-124
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• 2007

Adenoviral vectors are among the most promising vectors available for human gene therapy. However, the use of recombinant adenoviral vectors, including replicationcompetent adenovirus (RCA), raises a variety of safety concerns in relation to the development of new therapies based on gene therapy. To examine how organic compounds change in rat plasma following the injection of adenovirus, $\beta$-galactosidase expressing recombinant adenovirus (designated rAdLacZ) or RCA, we investigated the content of fatty acids (FAs), which are important biochemical indicators in pathological conditions. Pattern recognition analysis on the level of FAs in rat plasma is described for the visual discrimination of adenovirus infection groups from normal controls. Plasma FAs from four control rats (normal group), and from four rats with rAdLacZ infection and six rats with RCA infection (the two abnormal groups), were examined by gas chromatography-mass spectrometry in selected ion monitoring modes as their tert-butyldimethylsilyl derivatives. In total, 20 FAs were positively detected and quantified. The results of the Student's t-test on the normal mean of two abnormal groups, the levels of three FAs (p<0.05) from rAdLacZ group and eleven FAs (p<0.05) from RCA group were significantly different. When star symbol plotting was applied to the group mean values of 20 FAs after normalization to the corresponding normal mean values, the resulting eicosagonal star patterns of the two infected groups were distorted into similar shapes, but were distinguishable from each other. Thus, these approaches will be useful for screening and monitoring of diagnostic markers for the effects of infection following the use of adenoviral vectors in gene therapy.

• Fashion & Textile Research Journal
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• v.17 no.2
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• pp.265-277
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• 2015

The aim of this study was to successfully reflect human body changes on the transformation of the virtual body within 3D virtual fitting spaces. For this purpose, existing problems of shape transformation of the virtual body were analyzed and regression equations which provides useful basic data for transformation of the virtual body that can be applied usefully to the 3D virtual fitting system was developed. Necessary data for the analyses were body measurement and 3D scan data of women with average physical form between the ages of 40 through 49. The reason that we used human body changes of the female subjects in their forties was based on the recognition that fundamental female body changes start to occur from age of forty. Body shapes were largely divided into 3 groups according to obesity which was found to be the biggest factor of shape change. Seven factors were extracted based on factor analysis of 47 body measurement categories and regression equations were created to extract specific measurements for each BMI group based on these seven factors. The major contribution of this paper can be summarized as follows. First, the regression equations to extract specific measurements based on the 7 representative variables remediated existing problem of virtual bodies as it increased the number of body shape transformation areas. Second, the regression equations helped to overcome the problem of current failing to reflecting changes in body cross-section shape based on simple girth measurements based on analysis of cross-section distances.

• Journal of Convergence for Information Technology
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• v.10 no.8
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• pp.15-22
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• 2020

The 4th industrial revolution and the rapid change in the data environment revealed technical limitations in the existing relational database(RDB). As a new analysis method for unstructured data in all fields such as IDC/finance/insurance, interest in graph database(GDB) technology is increasing. The graph database is an efficient technique for expressing interlocked data and analyzing associations in a wide range of networks. This study extended the existing RDB to the GDB model and applied machine learning algorithms (pattern recognition, clustering, path distance, core extraction) to detect new abnormal signs. As a result of the performance analysis, it was confirmed that the performance of abnormal behavior(about 180 times or more) was greatly improved, and that it was possible to extract an abnormal symptom pattern after 5 steps that could not be analyzed by RDB.

• Journal of Oriental Medical Thermology
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• v.8 no.1
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• pp.33-38
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• 2010

Purpose : Dam-eum and women about this disease is closely related to the fact that research is not developed encounter the report. The purpose of this disorder is associated with women with Dameum hew to evaluate the affects around Dongulbogam Search and compare some of the knowledge gained. are reported. Methods : Donguibogam focused on search and related papers were. Results : In Donguibogam Dam-eum(痰), Yoen(涎), Eum(飮) to distinguish, but the reason was that one, 痰飮 sap needed to nutritious the body, such as a material or normal for various reasons by the sap from the leaves to cause disease states may be the cause. Conclusion: Digestive, respiratory-related, but now the recognition of the Dameum changes in diet and lack of exercise, stress, lifestyle diseases due to climate change are increasing asa Dameum and women in the pathogenesis of disease high enough proportion of the treatment for Dameum Applications will be denied a lot of disease is considered.

• Bulletin of the Korean Chemical Society
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• v.33 no.6
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• pp.1839-1844
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• 2012

We have investigated binding between wild-type and mutant Heat Shock Factor (HSF) DNA binding domains (DBDs) with 17-bp HSE containing a central 5'-NGAAN-3' element by equilibrium analytical ultracentrifugation using multi-wavelength technique. Our results indicate that R102 plays critical role in HSE recognition and the interactions are characterized by substantial negative changes of enthalpy (${\Delta}H^0_{\theta}=-9.90{\pm}1.13kcal\;mol^{-1}$) and entropy (${\Delta}S^0_{\theta}=-12.46{\pm}3.77cal\;mol^{-1}K^{-1}$) with free energy change, ${\Delta}G^0_{\theta}$ of $-6.15{\pm}0.03kcal\;mol^{-1}$. N105 plays minor role in the HSE interactions with ${\Delta}H^0_{\theta}$ of $-2.54{\pm}1.65kcal\;mol^{-1}$, ${\Delta}S^0_{\theta}$ of $19.28{\pm}5.50cal\;mol^{-1}K^{-1}$ and ${\Delta}G^0_{\theta}$ of $-8.35{\pm}0.05kcal\;mol^{-1}$, which are similar to those observed for wild-type DBD:HSE interactions (${\Delta}H^0_{\theta}=-3.31{\pm}1.86kcal\;mol^{-1}$, ${\Delta}S^0_{\theta}=17.38{\pm}6.20cal\;mol^{-1}K^{-1}$ and ${\Delta}G^0_{\theta}=-8.55{\pm}0.06kcal\;mol^{-1}$) indicating higher entropy contribution for both wild-type and N105A DBD bindings to the HSE.

• The Korean Journal of Rehabilitation Nursing
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• v.5 no.2
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• pp.217-225
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• 2002

Purpose : This research is aimed at developing a substantive theory related to the experience of adjustmented hemodialysis patients by identifying and analyzing the nature, process, and types of adjustment experienced by them. For this purpose, this study takes a grounded theory approach. Method : Data were collected from April, 2002 through September, 2002 through in-depth interviews and close observations of eleven hemodialysis patients who have experienced adjustment. With their consent, the interviews were recorded by audio tapes and later transcribed. Observation memos were also prepared on the subjects' behavior during the interviews. Data collection continued until saturated. The data were analyzed into concepts, subcategories, and categories with the open coding process. The axial coding was done to identify the relationships of the concepts and categories. And the selective coding was done to develop a core category, which is the central phenomenon of the hemodialysis patients who experienced adjustment. Result : This process resulted in 88 concepts, which may be grouped into 24 subcategories and 6 core categories. The 6 categories, in fact, depict the process of changes the patients experience from the sense of crisis, self-control, new life meaning, support system, coping ability, and quality of life improvement. And its core is self-control, which encompasses the dynamic stages from recognition, change attempts, belief solidification, and empowerment. Conclusion : The results of the research offer the following implications. First, research on adjustment is a formative stage in nursing, it is imperative for nursing researchers to develop them, which may be more relevant to nursing. Second, it is important to develop nursing intervention techniques that may be most effective in adjustment of hemodialysis patients and at the same time for each stage of changes taking place in adjustmented hemodialysis patients.

• Hwankyungkyoyuk
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• v.20 no.1
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• pp.90-105
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• 2007

The purpose of this study is to find the influence of environmental issue analysis instruction on the environmental decision making ability for grade 5 elementary school students. The study was done through pre and post testing control group structure. The object of this study is grade 5 of I elementary school students which were divided into 35 student test group and 54 student control group. Through studying references, the selection standard of appropriate environment issue and the environmental issue analysis instructing objective. Conducted the environment issue instructing based on the selected environment issue and instructing objective. The classes were held in total of 6 sessions in the chapters related to class objective and class content within the curriculum. The pre and post testing was done using environment decision making ability test sheet which was reconstructed by myself and the results were analyzed by t-test. As a result of comparing pre and post testing the students in test group showed significant results in the processes of problem recognition, evaluation of alternatives, behave planing (p<.001). As a result of comparing the differences of environment decision making ability of pre and post test of test group and control group, it showed significant results in the process of evaluation of alternatives(p<.00l). The environment issue analysis class has positive influence on the environment decision making abilities of the students but since the outcome of environment decision making ability is lower, there is a need for long term environment education plan and further studies to find whether the environment issues within the textbook is appropriate in the elementary student level, useful school aspect and the influence of environment issue analysis class on the change of values for individuals.