• 한국수산과학회지
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• 제32권2호
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• pp.144-148
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• 1999

상업적 규모의 순환여과식 탱크에서 사료 공급 횟수에 따른 산소 소비량의 변화를 조사하였다. 실험에 이용된 사육 장치는 순환여과식 시설내의 원형 콘크리트 탱크 (직경 270cm, 높이 80cm)를 이용하여 평균 체중 400g의 나일틸라피아 (Oreochromis niloticus)를 390kg 방양하여 실험에 이용하였다. 우선 3일간 사육 어류를 절식시킨 후 기초대사율을 측정하였고, 사료 공급 횟수를 1회, 2회, 5회 그리고 9회로 나누어 공급한 후 산소 소비량의 일간 변화를 측정하였다. 절식 상태에서 산소 소비량 (기초대사율)은 평균 $39.31{\pm}4.4mg\;O_2/kg$ fish/hr이였고 사료 공급횟수에 따른 1일 평균 산소 소비량은 1회, 2회, 5회 및 9회 공급시 각각 $79.9{\pm}21.5,\;81.3{\pm}14.8,\;84.2{\pm}9.9$ 및 $98.9{\pm}11.0mg\;O_2/kg$ fish/hr을 소비하는 것으로 나타나 사료 공급 횟수가 증가함에 따라 평균 산소 소비량은 증가하였으나, 유의적인 차이는 나타나지 않았다. 사료 공급후 최대 산소 소비량에 도달하는 시간은 사료 공급후 30$\~$50분으로 나타나 사료공급 횟수와는 유의적인 상관 관계가 없는 것으로 나타났다. 사료공급 횟수에 따른 산소 소비량의 최대치는 1회, 2회, 5회 및 9회 공급시에 각각 161.2, 123.4, 111.1 및 111.1 mg $O_2/kg$ fish/hr을 소비하는 것으로 나타나 하루에 1회 공급하였을 때 최대 산소 소비량이 가장 높은 것으로 나타났다.

• Membrane and Water Treatment
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• 제9권2호
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• pp.123-128
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• 2018

Common aquaculture practices include the use of certain pharmaceuticals such as antibiotics in avoiding diseases and promoting a healthier growth of the culture. The aim of this study is to monitor and assess the influence of different low oxytetracycline concentrations on the transformation of nitrogen compounds under aeration condition in a lab-scale recirculating aquaculture system (RAS). Over $1mg\;L^{-1}$ dose of oxytetracycline to aquaculture had induced ammonia($NH_4-N$), nitrate($NO_3-N$), soluble COD accumulation in RAS. In addition, nitrous oxide ($N_2O$) emission from RAS was significantly reduced during the oxytetracycline dose periods. After ceasing the dose of oxytetracycline, ammonia oxidation and nitrous oxide re-emission were observed. This observation indicated that low concentrations of oxytetracycline could affect the nitrogen species in RAS. Also, the emission mechanisms of $N_2O$ may not be only dependent on nitrification process but also dependent on denitrification process in our RAS system.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.371-378
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• 2011

Juvenile red seabream (mean body weight 29.0 g) were reared in recirculating culture systems with three different biofilter media, sand (SF), polystyrene microbeads (PF), and Kaldnes beads (KF). The efficiencies of the three different biofilter media were also tested. The SF was fluidized, and the PF and KF were trickled. All treatments were duplicated. The volumetric removal rates of total ammonia nitrogen by SF, PF, and KF were 193.8, 183.9, and 142.6 g $m^{-3}day^{-1}$, respectively, and those of nitrite nitrogen ($NO_2$-N) were 113.4, 105.9, and 85.8 g $m^{-3}day^{-1}$, respectively. The TAN and $NO_2$-N removal rates of KF were lower than those of SF and PF (P < 0.05), but there was no significant difference in these rates between SF and PF (P > 0.05). Among the biofilters used, only KF showed total suspended solid (TSS) removal capacity. The TSS removal efficiencies of SF and PF were negative. The growth rates of fish in SF were significantly higher than those in KF but not higher than those in PF. There was no difference in growth rate between fish in PF and KF. The specific growth rate and feed conversion efficiency of red seabreams in KF were lower than those in SF and PF, but there were no significant differences between SF and PF. These results indicate that sand and polystyrene microbeads are recommended for red seabream culture in a recirculating system.

• 한국미생물·생명공학회지
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• 제49권3호
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• pp.432-439
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• 2021

Microbial community plays important roles in the culture environment of mud crab Scylla serrata. One of the environmental management efforts for the cultivation of S.serrata is by stabilizing microorganisms involved in nitrogen cycle process. The availability of dissolved inorganic nitrogen in its culture environment under a recirculating system closely relates to the nitrogen cycle, which involves both anaerobic and aerobic bacterial activities. Anaerobically, there are two major nitrogen compound degradation processes, i.e., denitrification and dissimilatory nitrate reduction to ammonium (DNRA). This study aimed to identify denitrifying and DNRA bacteria isolated from the recirculating cultivation of S. serrata. The water samples were collected from anaerobic filters called close filter system, which is anaerobically conditioned with the addition of varying physical filter materials in the recirculating mud crab cultures. The results showed that three denitrifying bacterial isolates and seven DNRA bacterial isolates were successfully identified. The phylogenetic analysis based on 16S rRNA gene of the denitrifying bacteria revealed that HIB_7a had the closest similarity to Stenotrophomonas daejeonensis strain MJ03. Meanwhile, DNRA bacterial isolate of HIB_92 showed a 100% similarity to Bacillus sonorensis strain N3, Bacillus vallismortis strain VITS-17, Bacillus tequlensis strain TY5, Geobacillus sp. strain DB24, Bacillus subtilis strain A1, and Bacillus mojavensis strain SSRAI21. This study provides basic information denitrifying and DNRA bacterial isolates identity which might have the potential to be applied as probiotics in aquaculture systems in order to maintain optimal environmental conditions.

• 한국수산과학회지
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• 제49권3호
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• pp.343-350
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• 2016

The sea cucumber Apostichopus japonicus is a commercially valuable aquaculture species in Korea. Aquaculture species require specific nursery culture conditions to increase survival and growth rates. Sea cucumbers hibernate during the high temperatures of summer and during the low temperatures of winter, and suboptimal temperature conditions decrease sea cucumber growth and survival rates. The natural South Korean environment is very unfavorable for culturing sea cucumber; therefore, developing a recirculating aquaculture system (RAS) capable of breeding and growing sea cucumber year-round is necessary. The aim of this study was to investigate growth performance of juvenile sea cucumber in a RAS. Growth and survival rates of juvenile sea cucumber were high during our 24-week experiment. Sea cucumber survival rates were 87.8-93.3%, and specific growth rates were 0.4689-0.7846.

• Environmental Engineering Research
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• 제20권4호
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• pp.377-382
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• 2015

The high concentration of nitrogen and phosphorus in wastewater incorporated with the ability to use carbon dioxide as the carbon source make the microalgae become more attractive in wastewater treatment process. This study evaluates the optimal conditions for the digestion of settelable solids from the recirculating aquaculture system to produce the biomass of the green microalga Scenedesmus sp. After solids separation, aerobic digestion of settleable solids under disperse condition produced nitrate as the final product of consequently ammonification and nitrification processes. With the optimal digestion procedure, nitrate concentration during aerobic digestion in 2000 mL vessel increased from $9.63{\pm}0.65mg\;N/L$ to $58.66{\pm}0.06mg\;N/L$ in 10 days. Thereafter, cultivation of Scenedesmus sp. was performed in 1000 mL Duran bottle with air bubbling. The highest Scenedesmus sp. specific growth rate of $0.321{\pm}0.01/d$ was obtained in treatment using liquid fraction after aerobic digestion as the whole culture medium for Scenedesmus sp. cultivation. With this study, digestion of $8,800{\pm}128.12mg\;dry\;weight/L$ of settleable solids from fish pond finally produced $1,235{\pm}21mg\;dry\;weight/L$ of Scenedesmus sp. biomass.

• Fisheries and Aquatic Sciences
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• 제6권4호
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• pp.187-193
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• 2003

The performance of a foam fractionator to remove TAN, $NO_2,\;NO_3$, TSS, protein, and $PO_4-P$ at different superficial air velocities and foam overflow heights was evaluated in a lab-scale seawater recirculating system for culture of Korean rockfish (Sebastes schlegeli). The foam overflow rates increased with the increase of superficial air velocities, but decreased with the increase of foam overflow heights. Concentrations of all the water quality variables in the foam condensates increased with the increase of foam overflow height, but decreased with the increase of superficial air velocities. TSS, protein, and phosphate enrichment factors were within the range of 6.4-39.4, 1.6-7.3 and 1.2-3.9, respectively. Low values of TAN, $NO_2,\;and\;NO_3$ enrichment factors were obtained and they indicate that foam fractionation is rot an effective way to remove dissolved inorganic nitrogen. The calculated maximum daily removal values for TSS and protein were 10.9 and 1.4g, respectively.

• 한국수산과학회지
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• 제28권4호
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• pp.457-468
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• 1995

현재 유수식 방법으로 양식되고 있는 넙치(Paralichthys olivaceus)의 육상수조 양식에서 환수율, 월동시가온 및 양식배수 등의 문제를 해결하기 위하여, 실제 양식장에 설치한 반폐쇄식 순환여과 사육시스템을 이용하여 1992년 2월부터 1994년 1월까지 2년 동안(실험 I , 실험II) 넘치를 사육하면서 그 실용성을 연구하였다. 실험 I에서는 평균전장 7.5cm, 평균체중 3.4g, 실험II에서는 각각 5.0cm, 1.8g의 종묘를 사용하였다. 실험 I의 사육수 용존 무기태질소량은 $NH_4-N\;0.247-0.512ppm,\;NO_2-N\;0.010-0.043ppm,\;NO_3-N\;0.108-0.342ppm$ 실험II에서는 각각 0.091-0.715ppm, 0.002-0.045 ppm, 0,007-0.277 ppm 범위에서 변화하였다. 일간섭식률은 실험 I $0.67-2.41\%$, 실험II $0.69-2.22\%$, 사료효율은 각각 $34.8-59.8\%,\;40.5-88.4\%$였다. 실험 I에서의 어체는 사육 340일만에 전장 40.0-42.8cm, 체중 695.0-852.6g으로 성장하였고, 실험II에서는 사육 365일후에 전장 36.7-39.7cm, 체중 552.4-706.4g으로 자라났다. 사육종료시 어체의 평균 생존율은 실험 $192.0\%$, 실험II $96.0\%$였다. 어체의 체표면적이 수조바닥을 덮는율(covering rate)은 넙치의 수용밀도의 지표로 활용 가능하였다. 사육시 어체의 최대 수용량은 실험 I에서 덮는율 2.2, 중량 $34.1kg/m^2$였고, 실험II에서는 각각 $2.6,\;36.3kg/m^2$였다. 순환여과식 양식시스템은 현행의 유수식 양식시스템에 비해 양식 생산성 및 연안 환경오염 방지에 기여도가 높은 양식방법으로 평가되었다.

• 한국수산과학회지
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• 제44권6호
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• pp.747-752
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• 2011

The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).

• 한국수산과학회지
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• 제14권4호
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• pp.233-238
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• 1981

1981년 여름 여과조가 없는 순환사육장치를 설치 이용하여 잉어의 고밀도 실험 사육을 시행하였다. 평균 0.6g까리 잉어 치어 약 10,000미,20,000미,30,000미를 약 $7m^2$되는 3게의 사육 탱크에 각각 수용하여 평균 수온 $25^{\circ}C$ 전후에서 실험 사육한 결과10,000이 군에서는 1일 개체성장률 $3.7\%$였고 20,000미, 30,000미군에서는 각각 $2.8\%$및 $3.1\%$로 이들 사이에는 거의 차이가 없었다. 이번 사육 기간동안 대부분의 시기는 식물성 Plankton의 발생에 의하여 수색이 짙은 녹색으로 유지되었다. 특히, 본 보험 기간동안 $5{\sim}7ppm$ 정도의 높은 총 암모니아 농도에도 불구하고 모두 활발히 사료를 먹으면서 성장을 했다. 그리고, Green water 내에서는 밝은 물에 비해서 Columnaris병의 발병이 현저하게 억제됨을 알 수 있었다. 또, 이 시기중의 다른 1개의 탱크($7m^2$)에서 1981년 8월 19일부터 9월 27일까지 평균 352.2g 되는 잉어 446미를 수용하여 40일간 사육한 결과 평균486.3g으로 자랐고, 1일 평균 개체 성장률은 $0.8\%$로 나타났다. 이것은 대조로 실험한 밝은 물에서의 1일 성장률 $1.0\%$에 비해서 다소 성장이 지연된 결과였다. 따라서, 이 장치에서 산업적으로 생산을 하기 전에 종 더 암모니아양 및 Columnaris 병 등의 문제점을 해결하기 위한 실험적 연구가 요망된다고 느껴진다.