• Journal of Embryo Transfer
• /
• v.32 no.3
• /
• pp.73-79
• /
• 2017

Transplantation is considered to be a very useful approach to improve human welfare and to prolong life-span. Heterologous organ transplantation using pig organs which are similar to human beings and easy to make mass-production has known as one of the alternatives. To ensure potential usage of the pig organ for transplantation application, it is essentially required to generate transgenic pig modifying immuno-related genes. Previously, we reported production of heterozygous ${\alpha}1,3$-galactosyltransferase (GalT) knock-out and human membrane cofactor protein (MCP) expressing pig ($GalT^{-MCP/+}$), which is enforced for suppression of hyperacute and acute immunological rejection. In this study, we reported generation of homozygous pig ($GalT^{-MCP/-MCP}$) by crossbreeding $GalT^{-MCP/+}$ pigs. Two female founders gave birth to six of $GalT^{-MCP/-MCP}$, and seven $GalT^{-MCP/+}$ pigs. We performed quantitative real-time PCR, western blot, and flow cytometry analyses to confirm GalT and MCP expression. We showed that fibroblasts of the $GalT^{-MCP/-MCP}$ pig do not express GalT and its product Gal antigen, while efficiently express MCP. We also showed no expression of GalT, otherwise expression of MCP at heart, kidney, liver and pancreas of transgenic pig. Taken together, we suggest that the $GalT^{-MCP/-MCP}$ pig is a useful candidate to apply xenotransplantation study.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.24 no.2
• /
• pp.397-409
• /
• 2014

According to the Personal Information Protection Act(PIPA) which is legislated in March 2011, the individual or company that handles personal information, called Personal information processor, should encrypt some kinds of personal information kept in his Database. For convenience sake we call it DB Encryption in this paper. Law enforcement and the implementation agency accordingly are being strengthen the supervision that the status of DB Encryption is being properly applied and implemented as the PIPA. However, the process of DB Encryption is very complicate and difficult as well as there are many factors to consider in reality. For example, there are so many considerations and requirements in the process of DB Encryption like pre-analysis and design, real application and test, etc.. And also there are surely points to be considered in related system components, business process and time and costs. Like this, although there are plenty of factors significantly associated with DB Encryption, yet more concrete and realistic validation entry seems somewhat lacking. In this paper, we propose a realistic DB Encryption Assurance Framework that it is acceptable and resonable in the performance of the PIPA duty (the aspect of the individual or company) and standard direction of inspection and verification of DB Encryption (the aspect of law enforcement).

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2010.05a
• /
• pp.451-453
• /
• 2010

The world is rapidly evolving in the future society is based on intelligence. In such circumstances, USN(Ubiquitous Sensor Network) to implement the future ubiquitous(Ubiquitous) society have been highlighted as a key infrastructure. For realization of Ubiquitous computing(Ubiquitous Computing), The data recognized from each sensors must be collected on real-time and transferred to applied service so that they may be used as data for providing service to users. USN sensor data on the use for using a numerous sensor data provision service, service provider can publish its service in standardized registry for users to search and use the service registry. However, in previous studies using Web services standards WS-Eventing, and UDDI(Universal Description, Discovery, and Integration) as USR(USN Service Registry) for USN Application Service is unnecessary or insufficient. In this paper, data from sensors that provide information to service providers register their services and use the information for service users to explore and views on the registry of the system was designed and implemented.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2010.05a
• /
• pp.871-874
• /
• 2010

Needs for Smart Grid development are increasing all over the world as a solution to its problem according to depletion of energy resources, climatic and environmental rapidly change and growing demand for electrical power. Especially decentralized power is attracting world's attention. In this mood a new era for a unit scale of decentralized power environment is on its way in building. However there is a problem to have to be solved in the uniformity of power quality because the amount of power generated from renewable energy resources such as wind power and solar light is very sensitive to climate fluctuation. And thus this paper tries to suggest an energy management method on basis of real time monitoring for meteorological data. In the current situation of lacking in USN-based killer application in Smart Grid field, this paper proposes the USN-based DER management system which collects the meteorological data and control power system througout utilizing wireless sensor network technique this business. This communication technique is regarded to be efficient in aspects of installation cost and tits maintenance cost. The proposed EMS model embodies the method for predicting the power generation by monitoring and analyzing the climatic data and controling the efficient power distribution between the renewable energy and the existing power. The ultimate goal of this paper is to provide the technological basis for achieving zero-energy house.

• The Transactions of the Korea Information Processing Society
• /
• v.4 no.10
• /
• pp.2461-2469
• /
• 1997

Target detection and recognition problems, in which neural networks are widely used, require translation invariant and real-time processing in addition to the requirements that general pattern recognition problems need. This paper presents a novel architecture that meets the requirements and explains effective methodology to train the network. The proposed neural network is an architectural extension of the shared-weight neural network that is composed of the feature extraction stage followed by the pattern recognition stage. Its feature extraction stage performs correlational operation on the input with a weight kernel, and the entire neural network can be considered a nonlinear correlation filter. Therefore, the output of the proposed neural network is correlational plane with peak values at the location of the target. The architecture of this neural network is suitable for implementing with parallel or distributed computers, and this fact allows the application to the problems which require realtime processing. Net training methodology to overcome the problem caused by unbalance of the number of targets and non-targets is also introduced. To verify the performance, the proposed network is applied to detection and recognition problem of a specific automobile driving around in a parking lot. The results show no false alarms and fast processing enough to track a target that moves as fast as about 190 km per hour.

• The Journal of Korean Society for School & Community Health Education
• /
• v.6
• /
• pp.89-107
• /
• 2005

The purposes of this study were to investigate the recognition and the needs and problems of in-service education for school nurse, and to suggest the desirable guidelines, for supples the basic data of in-service education for school nurse to upgraded the quality as school nurse's professional specialist. The subjects of this study were 376 school nurses who were working in Jollanamdo. The research instruments used in this study was 'Needs of In-service Education questionnaire'. 305 collected Data were analyzed with the frequency analysis, $x^2$-test. The conclusions were as follows; First of all, the most important motives for the school nurses to participate in-service education are the enhancement of their specialties on teaching profession, self-realizations as educators, and improvement of health teaching skill. However, the motives to obtain the skill for school management or to obtain a high rank qualification and promotion are quite low. School nurses are generally satisfied with duration, time, place of in-service education, But they are not satisfied with contents of in-service education, professional specialist and understanding of real educational situation of the instructors. On the urgent problem of school nurses, promotion of health teaching skill was highest in the rank, and establishment of firm educational philosophy and a sense of teaching profession, proceed to university and graduate school ranked next, respectively. Second, the need of a school nurses on in-service education direction ranked the application of teachers' character and need, practicable and concrete educational programs, planning of school health development, reinforcement of health education, expansion of practical knowledge and on reflection thought, respectively. The need of a school nurses on in-service education contents(major part) ranked health education, health promoting program of student, knowledge and practice of practical medicine and oriental medicine, consultation process, health education of advanced country, respectively. The need of in-service education supervisory organization, the need for a cities provinces educational office was highest in the rank. The need of in-service education type, duty training ranked high, and abroad training, qualification training, general training ranked next. the need for specialist for lecturer of in-serve education ranked among the highest, along with school nurses and university professor. The need of school nurses on education method(duplication answer), need for conference and discussion teaching was highest in the rank. The need on evaluation method, evaluation through a examination ranked the highest. On the needs of in-service education times, need for vacation during the winter and summer was the highest. As for the duration, 31 to 60 hours in duration of in-service education was need most, and most school nurses need cities and provinces in-service training institute as the location of in-service education. On the organization size, need for 21 to 30 people was the highest, where as need for 41 people was relatively low. Lastly, on the problem of in-service education for school nurses, lack of opportunity of in-service education for school nurses was highest in the rank, and improperness of in-service education contents and method, lack of incentive ranked next, respectively.

• Journal of Korean Society of Transportation
• /
• v.26 no.4
• /
• pp.173-184
• /
• 2008

Development of an accident estimation model based on accident data can be made after accident occurrences. However, the taking of historical accident data is not easy, and there have been differences between real accident data and police-reported accident data. Also, another difficult shortcoming is that historical traffic accident data better consider driver behavior or intersection characteristics. A new method needs to be developed that can predict accident occurrences for traffic safety improvement in black spots. Traffic conflict decision techniques can acquire and analyze data in time and space, requiring less data collection through investigation. However, there are shortcomings: as existing traffic conflict techniques do not operate automatically, the analyst's opinion could easily affect the study results. Also, existing methods do not consider the severity of traffic conflicts. In this study, the authors presented traffic conflict decision criteria which consider conflict severity, including opposing left turn traffic conflict and cross traffic conflict decision criteria. In order to test these criteria, the authors acquired three signalized intersection images (two intersections in Sungnam city and one intersection in Paju) and analyzed the acquired images using image processing techniques based on individual vehicle tracking technology. Within the analyzed images, level 1 conflicts occurred 343 times over three intersections. Some of these traffic conflicts resulted in level 3 conflict situations. Level 3 traffic conflicts occurred 25 times. From the study results, the authors found that traffic conflict decision techniques can be an alternative to evaluate traffic safety in black spots.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.27 no.1
• /
• pp.70-76
• /
• 2017

Objectives: This on-site study was performed to evaluate the reduction efficiency of an air cleaner on particulate matters and biological agents in a swine facility. Materials and Methods: Particulate matter was measured using a real-time monitoring recorder and biological agents were sampled with a one-stage impactor and then analyzed based on the microbial culture method. An experimental process for the reduction effect on airborne pollutants through air cleaner operation consisted of three conditions: no treatment, wet scrapper by water spray and wet scrapper by disinfectant spray. Results: Geometric mean levels of particulate matter(TSP, $PM_{10}$, $PM_{2.5}$ and $PM_1$) were presented at $1,608{\mu}g/m^3$, $1,373.8{\mu}g/m^3$, $401.8{\mu}g/m^3$ and $144.5{\mu}g/m^3$ for no treatment; $1,503{\mu}g/m^3$, $1,017{\mu}g/m^3$, $159.4{\mu}g/m^3$ and $69.8{\mu}g/m^3$ for wet scrapper by water spray; and $1,222.17{\mu}g/m^3$, $477.17{\mu}g/m^3$, $33.2{\mu}g/m^3$ and $11.1{\mu}g/m^3$ for wet scrapper by disinfectant spray, respectively. In the case of biological agents, the geometric averaged concentrations of total airborne bacteria and fungi were as follows: $45,371cfu/m^3$ and $13,474cfu/m^3$ for no treatment, $43,286cfu/m^3$ and $8,610cfu/m^3$ for wet scrapper by water spray, and $2,440cfu/m^3$ and 1,867 cfu/ for wet scrapper by disinfectant spray, respectively. Regardless of particulate matter and biological agent, the highest concentrations were found for no treatment, while the lowest concentrations were found with wet scrapper by disinfectant spray. Conclusions: Based on the results obtained from this on-site evaluation, there was a significant reduction effect on particulate matter and biological agents through the application of an air cleaner in this study.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.4
• /
• pp.34-42
• /
• 2017

Since existing nonintrusive appliance load monitoring (NIALM) studies assume that voltage fluctuations are negligible for load identification, and do not affect the identification results, the power factor or harmonic signals associated with voltage are generally not considered parameters for load identification, which limits the application of NIALM in the Smart Home sector. Experiments in this paper indicate that the parameters related to voltage and the characteristics of harmonics should be used to improve the accuracy and reliability of the load monitoring system. Therefore, in this paper, we propose an improved NIALM method that can efficiently analyze the types of household appliances and electrical energy usage in a home network environment. The proposed method is able to analyze the energy usage pattern by analyzing operation characteristics inherent to household appliances using harmonic characteristics of some household appliances as recognition parameters. Through the proposed method, we expect to be able to provide services to the smart grid electric power demand management market and increase the energy efficiency of home appliances actually operating in a home network.

• Resources Recycling
• /
• v.29 no.1
• /
• pp.25-34
• /
• 2020

Electrochemical Quartz Crystal microbalance is a tool that is capable of measuring nanogram-scale mass change on electrode surface. When applying alternating voltage to the quartz crystal with metal electrode formed on both sides, a resonant frequency by inverse piezoelectric effect depends on its thickness. The resonant frequency changes sensitively by mass change on its electrode surface; frequency increase with metal dissolution and decrease with metal deposition on the electrode surface. The relationship between resonant frequency and mass change is shown by Sauerbrey equation so that the mass change during metal dissolution can be measured in real time. Especially, it is effective in the case of reaction mechanism and rate studies accompanied by precipitation, volatilization, compound formation, etc. resulting in difficulties on ex-situ AA or ICP analysis. However, it should be carefully considered during EQCM experiments that temperature, viscosity, and hydraulic pressure of solution, and stress and surface roughness can affect on the resonant frequency. Application of EQCM was shown as a case study on leaching of platinum using aqueous chlorine for obtaining activation energy. A platinum electrode of quartz crystal oscillator with 1000 Å thickness exposed to solution was used as leaching sample. Electrogenerated chlorine as oxidant was purged and its concentration was controlled in hydrochloric acid solution. From the experimental results, platinum dissolution by chlorine is chemical reaction control with activation energy of 83.5 kJ/mol.