• Korea-Australia Rheology Journal
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• v.21 no.2
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• pp.127-133
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• 2009

Hemorheological results may be influenced by the time between blood sampling and measurement, and storage conditions (e.g., temperature, time) during sample delivery between laboratories may further affect the resulting data. This study examined possible hemorheological alterations subsequent to storage of rat and dog blood at room temperature ($22^{\circ}C$) or with cooling ($4{\sim}10^{\circ}C$) for 2, 4, 6, 24, 48 and 72 hours. Measured hemorheological parameters included hematological indices, RBC aggregation and RBC deformability. Our results indicate that marked changes of RBC deformability and of RBC aggregation in whole blood can occur during storage, especially for samples stored at room temperature. The patterns of deformability and aggregation changes at room temperature are complex and species specific, whereas those for storage at the lower temperature range are much less complicated. For room temperature storage, it thus seems logical to suggest measuring rat and dog cell deformability within 6 hours; aggregation should be measured immediately for rat blood or within 6 hours for dog blood. Storage at lower temperatures allows measuring EI up to 72 hours after sampling, while aggregation must be measured immediately, or if willing to accept a constant decrease, over 24~72 hours.

• The Korean Journal of Food And Nutrition
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• v.9 no.2
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• pp.189-200
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• 1996

The purpose of this study was to find the effect of honey on the sugar metabolism In Sprague-Dawley rats. All experimental rats were fed ad libitum, for seven weeks, 68% saccharide diet anti 10% or 20% honey from acacia, sumac and miscellaneous flower honey, respectively, and sucrose. The amount of glucose in whole blood was increased in all groups fed with honey except the group fed with 10% sumac honey solution. The amount of fructosamine in serum of rat taken diet with honey solution was increased in comparison with normal group, control group, sucrose group. The amount of fructosamine in serum of rat taken diet with honey solution was high in order of sumac honey > miscellaneous flower honey > acacia honey. The amount of lactate and pyruvate in whole blood of rat taken diet with sucrose group or honey group were decreased in comparison with the normal group and control group. The amount of $\beta$-hydroxybutyrate in serum of rat taken diet with sucrose or honey was increased in comparison with the normal group, control group.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.13-20
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• 1991

For this experiment frist the fermented Soybean Meju was prepared by domestic ordinary method of Korean and concentrated Meju suspensoidal extract was made for toxicological test sample on mice and rat. Even the oral dose of 10g extract per kg of mice had not any acute toxicity. So the median lethal dose(LOD50) is lilely to be much greatr or not to be. In comparison of the average weight gain rate for 4 weeks the control and test groups of 0.308g Ex/rat kg and 9.24g Ex/rat kg daily were the much the same as about 19% but 3.08g Ex/rat kg group waas exceptionally 2.7% higher than control For this period all test groups did not show any abnormality in appearance and anatomic findings. In serum GPT GOT alkaline phosphatase and blood urea nitrogen value there were no difference significantly between control and the test groups of 0.308g/rat kg and 3.08g/rat kg but 9.24g/rat kg group was some what higher than control. Especially in cholesterol alkaline phosphatase and blood urea nitrogen value 9.24g/rat kg group or 28days had 100mg/이 37.5u 32mg/이 respectively which were increased significantly. The pathological findings of all test groups especially 9.24g/kg group did not showed any characterized sign of acute or subacute liver damage or intestine lesion. So it is supposed that there is not any tocixants originated from some molds in fermented Korean domestic Meju which is prepared traditionally in winter.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.1
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• pp.27-33
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• 1996

납중독에 대한 마늘의 antidotic effect를 조사하기 위한 4주간의 실험에서 , rat 체중 kg 당 중 (7일간) 1회초산납 100mg을 투여한 rat에 매일 마늘즙을 식이의 4%로 투여한 실험군(LG)과 초산납과 함께 중금속 해독제인 N-acetyl penicillamine을 매일 rat 체중 kg당 100mg 투여한 실험군 (LP)의 체중 증가율 비교에서 초산납만을 투여한 실험군(L)보다 LG군과 LP군 모두 유의성 있는 rat 성장률 개선효과 (각각 +13.3%과 +22.3%)를 보였다. L군 rat의 외관과 해부검사에서는 장기들(위, 간장, 신장)의 병변(damage)이 매우 미미하게 관찰되었으나 혈액검사에서 GOT, alkaline phos-phatase, blood uric acid, blood urea nitrogen, crea-tinine, bilitrubin 값이 유의적을 증가하여 납중독에 의한 장기(organs) 특히 신장 기능의 현저한 장해를 보였다. 그러나 LG군이 이들 측정값이 L군보다 유의성의 차로 낮은 값을 보여 LP군과 거의 비슷한 수준이었다. 특히 L군에서는 Pb 중독으로 인하여 hemo-globin 량이 정상 이하(10.37g/dl)로 감소되었으나 LG군에서는 L군에서와 같이 거의 정산 (12.32g/dl)을 유지하였다. L군의 혈액, 간장 및 신장의 납 합량은 각각 0.281, 0.250, 0.403ppm으로 대조군 보다 매우 높았으나 LG군은 대조군에 가깝게 그리고 LP군과는 거의 같은 수준(각각 0.182, 0.131, 0.253ppm)으로 낮아졌다. 이상의 실험 결과에서 마늘이 rat의 납중독에 미치는 효과를 N-acetyl penicillamine의 해독 효과와 비교할 때 마늘의 해독 효과라고 볼 수 있는 유의성있는 측정값들이 관찰되었다.

• Journal of Haehwa Medicine
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• v.5 no.2
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• pp.319-332
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• 1997

Experiments were performed to determine the effects of the Shiryungtang (SRT) liquid extract on the hypertension and the hyperlipidemia induced by cholesterol in Sprague-Dawley rat(SDR) and Spontaneous Hypertensive rats(SHR). The results were obtained as follows : 1. The blood pressures of Sample A, B were decreased significantly compared with control group. First day, in case of Sample B, the blood pressure depressed after 1 and 2 hour. 2. After 11days of feeding with SRT, the blood pressure was decreased significantly in the treated group as compared with the control group. 3. After 11days by fed with SRT, the cholesterol had no significant in all treated group compared with the control group. 4. After 11days by fed with SRT, the triglyceride had no significant in all treated group compared with the control group. 5. The cholesterol of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in all treated group compared with the control group. 6. The triglyceride of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in all treated group compared with the control group. 7. The HDL-cholesterol of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in all treated group compared with the control group. 8. The phospholipid of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in Sample B of the treated group. 9. The total lipid of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in Sample B of the treated group. 10. The total protein of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in Sample B of the treated group. 11. The albumin of hypercholesterolemic rat induced by feeding with cholesterol was decreased significantly in all treated group compared with the control group. With the above result, it is thought that Shiryungtang can be applied effectively to the hypertension and hyperlipidemia.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.2
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• pp.138-143
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• 1993

In this study the onion juice (2%) in diet fed rats simultaneously ingested lead acetate 100mg/ kg (OP group) showed more increased weight gain than single lead treated rats (P group). The OP group had also improved in the hemoglobin contents and biochemical analyzed values of blood including GPT, blood urea nitrogen and alkaline phosphatase, which were elevated in case of P group rats. The Pb content in the rats liver of OP group was lower than in the rats liver of P group. In the histopathological findings of liver cell OP group rats did not show any signs of liver damage as observed in P group rats that had degenerated hepatocytes, followed sinusoidal dilatation, perivascular hemorrhage and some necrosis of hepatic cells accompanied by increased Kuffer cell bearing dark brown pigment. In conclusion 2% onion juice diet in rat have somewhat antidotic effects on the lead intoxicated rats.

• The Journal of Dong Guk Oriental Medicine
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• v.2 no.1
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• pp.19-54
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• 1993

To see both $Sh{\grave{u}}nq{\grave{i}}daot{\acute{a}}nt{\bar{a}}ng$(dissipate phlegm and promote vital energy circulation) and $Hu{\grave{a}}y{\bar{u}}t{\bar{a}}ng$(blood circulation and disperse blood stasis) influencing on thrombosis, contusion-hyperemia, and hyperlipidemia, at first we measured the density of FDP, the quantity of fibrinogen, prothrombin time, and the number of platelet of rat taken thrombosis by endotoxin. Secondly we measured the increase-rate of "paw swelling", the number of platelet, the quantity of fibrinogen, and prothrombin time of rat taken contusion-hyperemia. And then we measured the quantity of total cholesterol in serum and of H.D.L-cholesterol and of triglyceride and of phospholipid and of ${\beta}-lipoprotein$, its weight, and the variation of the quantity of electrolyte of rat taken hyperlipidemia by the oral-injection of choleserol. As a result, we can conclude as follows : 1. Out of the test of thrombosis, we can recognize not only the noticeable increae of the number of platelet and the quantity of fibrinogen, but also the noticeable decrease of prothrombin time and the density of FDP in case of $Sh{\grave{u}}nq{\grave{i}}daot{\acute{a}}nt{\bar{a}}ng$-injected rat and $Hu{\grave{a}}y{\bar{u}}t{\bar{a}}ng$-injected rat. 2. Out of the test of contusion-hyperemia, we can recognize not only the noticeable increase of the number of platelet and the quantity of fibrinogen, but also the noticeable decrease of prothrombin time and "increase-rate of paw swelling" in case of $Sh{\grave{u}}nq{\grave{i}}daot{\acute{a}}nt{\bar{a}}ng$-injected rat and $Hu{\grave{a}}y{\bar{u}}t{\bar{a}}ng$-injected rat. 3. Out of the test of hyperlipidemia, at first we can recognize that test rat's weight increased as close as that of normal rat. And we can recognize the noticeable decrease of the triglyceride and phospholipid and ${\beta}-lipoprotein$." Also, in case of the variation of electrolyte we can recognize the decrease of calcium and potassium in $Sh{\grave{u}}nq{\grave{i}}daot{\acute{a}}nt{\bar{a}}ng$-injected rat, and of sodium and magnesium in $Hu{\grave{a}}y{\bar{u}}t{\bar{a}}ng$-injected rat. Thus, as the above-mentioned, in covering thrombosis, contusion-hypermia, and hyperlipidemia, the effect of $Sh{\grave{u}}nq{\grave{i}}daot{\acute{a}}nt{\bar{a}}ng$ and $Hu{\grave{a}}y{\bar{u}}t{\bar{a}}ng$ can be recognized. Granting that $Hu{\grave{a}}y{\bar{u}}t{\bar{a}}ng$ reveals its effectiveness in thrombosis and contusion-hyperemia, and $Sh{\grave{u}}nq{\grave{i}}daot{\acute{a}}nt{\bar{a}}ng$ in hyperlipidemia, it can be inferred that contusion-hyperemia is like "model of blood stasis form" as thrombosis and hyperlipidemia "phlegm-retention diseases form", and both phlegm-retention and blood stasis have correlation each other.

• YAKHAK HOEJI
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• v.26 no.4
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• pp.253-256
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• 1982

The organ distribution of mercury was examined in the rat after oral administration of a single dose of red mercuric sulfide (15mg Hg/kg). The concentration of total mercury in the organs and blood after 2, 4, 6, 8, 12, 24 and 72 hours of administration was determined by Quartz Tube Combustion-Gold Amalgamation Method. It was found that the maximal concentration of total mercury was in the kidneys and muscle within 24 hours and in the brain, heart, liver and blood within 48 hours. The descending order of the maximal organ and blood concentration was: kidneys(1.08ppm)>blood> muscle>heart>liver>brain. The accumulation states of total mercury in the rat organs were investigated by continuous administration of red mercuric sulfide (5mg Hg/kg/day) for 15 days. The mercury concentration increased progressively throughout the experimental period and the descending order of the highest level of mercury after 15 days was: kidneys (1.55ppm)>blood>liver. The concentration of alkyl mercury in brain, liver and kidneys also was measured after 7 and 15 days of consecutive administration of red mercuric sulfide (5mg Hg/kg/day). The concentration in the Kidneys and the liver was very low, but was significantly different from control group. The concentration in the brain was extremely low and was not significantly different from control group.

• Archives of Pharmacal Research
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• v.19 no.5
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• pp.348-352
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• 1996

The antioxidant efficacy of aspalatone, a new antithrombotic agent, has been recognized in the neurotoxic model and in the cardiotoxic model in proliminary studies. We examined the specific activity of antiosidnat enzyme in the rat blood following administrations of aspirin, maltol, aspirin together with maltol, salicylmaltol (major metabolite of aspalatone) and aspalatone, respectively. Our assessment showed that salicylmaltol, maltol, aspalatone enhanced antiperoxidative activity. In addition, neither aspirin nor combination of aspirin and maltol, showed any significant effect on the activity of antioxidant enzyme. Because $H_{2}$$O_{2}$ accumulation may stimulate the thrombogenesis in blood, the result suggests that the induction of blood antiperoxidative activity produced by aspalatone may have beneficial effects on the thrombogenesis.

• Current Optics and Photonics
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• v.1 no.5
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• pp.433-439
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• 2017

The rodent model has been used frequently to understand stroke pathophysiology, due to its low cost and the large spectrum of genetic strains available. Here, we present a diffuse speckle contrast analysis system (DSCA) with a $1{\times}2$ optical switch that was used to non-invasively assess cerebral blood flow (CBF) changes in the rat during intraluminal suturing for middle cerebral artery occlusion (MCAO) surgery. The blood flow index (BFI) in the left hemisphere was lower than that in the right hemisphere because the left middle cerebral artery was occluded. Furthermore, the performance of the DSCA system was compared with that of commercial laser Doppler flowmetry. The changes in the BFI measured by the two systems were correlated strongly. The DSCA system was less sensitive to motion artifacts and able to measure relatively deep tissue flow in the rat's brain. In conclusion, the DSCA system secured CBF monitoring during surgery in a rodent model without craniotomy.