• Title/Summary/Keyword: Rapid R&D

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Studies on the Pathogenicity of Aphelenchoides sp. and Rhabditis sp. attacking Cultivated Mushroom, Agaricus bisporus(Lange) Sing (Aphelenchoides sp.와 Rhabditis sp.의 양송이에 대한 병원성에 관한 연구)

  • Han, Yong-Sik;Park, Jung-Su;Kim, Dong-Soo;Shin, Kwan-Chul
    • The Korean Journal of Mycology
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    • v.1 no.1
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    • pp.3-11
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    • 1973
  • Four species of nematodes attacking mushroom beds were found in samples taken from 35 mushroom farms throughout Korea. These were Rhabditis sp., Aphelenchoides sp., Ditylenchus sp. and Aphelenchus sp. ,Rhabditis sp. was found from compost and casing from all mushroom farms and the frequencies of Aphelenchoides sp. was 31.4% in the both compost and casing. Both Ditylenchus sp. and Aphelenchus sp. showed 2.7% of frequencies in the compost, none in casing. Temperature and moisture content of compost affected pathogenicity of Aphelenchoides sp. on mushroom mycelia grown in compost. The higher temperature and moisture content the sooner the damage became apparent, and the more rapid was subsequent destruction of mycelia. There was no mycelial destruction at the lowest temperature of $10^{\circ}C$. Rhabditis sp. completely disintegrated mycelia grown in the compost, in the early stage, the numbers of Rhabditis sp. rose gradually and then increased suddenly to reach a peak but soon declined. At first, the pH of Rhabditis-infested spawned compost declined but then rose gradually as mycelia was disintegrated by nematodes. The trend in pH of infested unspawned compost was similar to those of uninfested, unspawned compost. Cultures inoculated with surface-disinfected dead Rhabditis sp. and with tap water used in the nematode extraction procedures showed no mycelial injury associated microorganisms containing within or outside the nematodes even though added by artificial wounding of the mycelia. Cultures artificially wounded showed no injury away from the wounds without the presence of living Rhabditis sp., such wounded mycelia slowly regenerated. On the other hand, artificial wounding accelerated the breakdown of mycelia in the presence of living Rhabditis sp.

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Molecular Cloning and Characterization of a New cDNA Encoding Hyoscyamine 6β-hydroxylase from Roots of Anisodus acutangulus

  • Kai, Guoyin;Chen, Junfeng;Li, Li;Zhou, Genyu;Zhou, Limin;Zhang, Lei;Chen, Yuhui;Zhao, Linxia
    • BMB Reports
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    • v.40 no.5
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    • pp.715-722
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    • 2007
  • A new full-length cDNA encoding hyoscyamine $6\beta$-hydroxylase (designated as aah6h, GenBank Accession No. EF187826), which catalyzes the last committed step in the scopolamine biosynthetic pathway, was isolated from young roots of Anisodus acutangulus by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of aah6h was 1380 bp and contained a 1035 bp open reading frame (ORF) encoding a deduced protein of 344 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.09 and a calculated molecular mass of about 38.7 kDa. Sequence analyses showed that AaH6H had high homology with other H6Hs isolated from some scopolamine-producing plants such as Hyoscyamus niger, Datura metel and Atropa belladonna etc. Bioinformatics analyses results indicated AaH6H belongs to 2-oxoglutarate-dependent dioxygenase superfamily. Phylogenetic tree analysis showed that AaH6H had closest relationship with H6H from A. tanguticus. Southern hybridization analysis of the genomic DNA revealed that aah6h belonged to a multi-copy gene family. Tissue expression pattern analysis firstly founded that aah6h expressed in all the tested tissues including roots, stems and leaves and indicated that aah6h was a constitutive-expression gene, which was the first reported tissue-independent h6h gene compared to other known h6h genes.

Properties of Mortar according to Gradation change of Electric Arc Furnace Oxidizing Slag Fine Aggregate made by Rapidly Cooled Method (급냉 전기로 산화슬래그 잔골재의 입도 변화에 따른 모르타르의 특성)

  • Kim, Jin-Man;Kwak, Eun-Gu;Choi, Sun-Mi;Kim, Ji-Ho;Lee, Won-Young;Oh, Sang-Youn
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.4
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    • pp.112-118
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    • 2012
  • Steel industry produces many by-products and wastes such as blast furnace slag, electric arc furnace slag, and converter slag. As in the case of rock, the main component of steel slag are CaO and $SiO_2$ ; further, steel slag is as alkaline as portland cement or concrete. Electric arc furnace oxidizing slag is possible to use as an aggregate for concrete ; however, it has been reclaimed because of it's expansibility caused by free CaO. Recently, a innovative rapid cooling method for melting steel slag has been developed in Korea, which reduces free lime content to minimum level and increases the stability of iron oxide. Therefore, this study describes the results of a series of research to utilize globular shape of electric arc furnace oxidizing slag fine aggregates made by rapidly cooled method for the construction industry by cooling rapidly melted slag from the steel industry. First of all, an experiment was carried out to investigate the quality characteristics of rapidly cooled electric arc furnace oxidizing slag fine aggregates in order to determine whether they can be applied to the construction industry. Then, by applying them to concrete of various particle sizes, we explored experimentally the desired condition to apply rapidly cooled electric arc furnace oxidizing slag fine aggregates to concrete.

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Analysis of Abroad Mid- to Long-Term R&D Themes and Market Information in the Geological Information and Mineral Resources Fields (지질정보 및 광물자원 분야 국외 중장기 연구개발 주제 및 시장정보 분석)

  • Ahn, Eun-Young
    • Economic and Environmental Geology
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    • v.52 no.6
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    • pp.637-645
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    • 2019
  • Due to the transformation to the intelligent information society, the rapid change of our life and environment is expected. The Ministry of Science and ICT (MSIT) and the National Research Council of Science and Technology (NST) introduced a five-year government supported research institution's planning and evaluation based on the mid-to long-term perspective. This study collects international benchmarking information including industry, academia, and research fields by collecting mid- and long-term strategy reports from public research institutes, surveys by experts from abroad universities and research institutes, and analyzing overseas market information reports. The British Geological Survey (BGS), the U.S. Geological Survey (USGS) and the japanese geological survey related institutes (AIST-GSJ) plans for three-dimensional national geological information, predictions of geological environmental disasters, and development of important metals and material in the low carbon economic transformation and in the era of the Fourth Industrial Revolution. The mid- and long-term program emphasizes basic and public research on geological information through abroad experts survey such as the IPGP-CNRS etc. The market analysis of the mining automation and digital map sectors has been able to derive the fields in which the role of public research institutes by the market is expected such as data collection on land and in the air, mobile or three-dimensional information production, smooth/fast/real-time maps, custom map design, mapping support to various platforms, geological environmental risk assessment and disaster management information and maps.

Ability to Resist Chloride Ion Penetration and Dry Shrinkage Evaluation of Magnesium Phosphate Ceramics (인산마그네슘 세라믹의 염소 이온 투과 저항성 및 길이변화 특성에 관한 성능 평가)

  • Ko, Jeong-Won;Yang, Wan-Hee;Park, Dong-Cheol
    • Journal of the Korea Institute of Building Construction
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    • v.17 no.4
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    • pp.341-348
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    • 2017
  • The performance degradation of concrete pavement by winter deicer is very serious in Korea, and its maintenance and rehabilitation brings a high expense. Therefore, a suitable method for rehabilitation of such concrete pavement and repair material of proper performance are required. In this study, the properties of compressive strength, ability to resist chloride ion penetration, and properties of dry shrinkage of magnesium phosphate ceramics were assessed to evaluate its applicability as a repair material for concrete pavement in Korea. As a result, the mortar flow showed a normal level of 190 mm, but the viscosity was high and the self-flow ability was poor. The setting time was 12 minutes, leading very rapid-hardening, and thus a prompt work was required. The compressive strength of mortar was 38.4MPa in 2 hours, 73.8MPa in 24 hours, and 111.0MPa in 28 days, showing a significant level. As a result of the test to chloride ion penetration resistance, mortar showed 143 Coulombs, and concrete showed 172.6 Coulombs, which fell under very low level. The drying shrinkage of MPC concrete in 40 days was below $60{\times}10-6$, and comparing with normal cement concrete, it showed the level below 1/10 of other concrete to secure an excellent volume stability. As above, magnesium phosphate ceramics has excellent strength performance, chloride ion penetration resistance, and volume stability, and this in the future shall be used in construction under the consideration of working time or workability, requiring further improvement for such performance.

Back Surface Field Properties with Different Surface Conditions for Crystalline Silicon Solar Cells (후면 형상에 따른 결정질 실리콘 태양전지의 후면전계 형성 및 특성)

  • Kim, Hyun-Ho;Kim, Seong-Tak;Park, Sung-Eun;Song, Joo-Yong;Kim, Young-Do;Tark, Sung-Ju;Kwon, Soon-Woo;Yoon, Se-Wang;Son, Chang-Sik;Kim, Dong-Hwan
    • Korean Journal of Materials Research
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    • v.21 no.5
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    • pp.243-249
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    • 2011
  • To reduce manufacturing costs of crystalline silicon solar cells, silicon wafers have become thinner. In relation to this, the properties of the aluminium-back surface field (Al-BSF) are considered an important factor in solar cell performance. Generally, screen-printing and a rapid thermal process (RTP) are utilized together to form the Al-BSF. This study evaluates Al-BSF formation on a (111) textured back surface compared with a (100) flat back surface with variation of ramp up rates from 18 to $89^{\circ}C$/s for the RTP annealing conditions. To make different back surface morphologies, one side texturing using a silicon nitride film and double side texturing were carried out. After aluminium screen-printing, Al-BSF formed according to the RTP annealing conditions. A metal etching process in hydrochloric acid solution was carried out to assess the quality of Al-BSF. Saturation currents were calculated by using quasi-steady-state photoconductance. The surface morphologies observed by scanning electron microscopy and a non-contacting optical profiler. Also, sheet resistances and bulk carrier concentration were measured by a 4-point probe and hall measurement system. From the results, a faster ramp up during Al-BSF formation yielded better quality than a slower ramp up process due to temperature uniformity of silicon and the aluminium surface. Also, in the Al-BSF formation process, the (111) textured back surface is significantly affected by the ramp up rates compared with the (100) flat back surface.

A Study on Required Competency for Each Field of Intellectual Property in the 4th Industrial Revolution Era (4차 산업혁명 시대의 지식재산 분야별 필요 역량 분석)

  • Park, Ki-Moon;Lee, Kyu-Nyo;Lee, Byung-Wook
    • 대한공업교육학회지
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    • v.45 no.2
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    • pp.108-130
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    • 2020
  • In order to cope with the rapid changes in science and technology and various societies and complex economies with the advent of the 4th Industrial Revolution, intellectual property education is essential above all. In this study, therefore, intellectual property and the required competences were derived and verified for each field. As for the research method, Delphi technique was used twice for literature review and experts, and the conclusions of the research are as follows. First, the field of intellectual property required in the era of the 4th Industrial Revolution was classified, through literature review and Delphi technique, in eleven sections such as IP-R & D consulting, IP information search analysis, IP entitlement, IP strategic planning, IP transaction, IP finance, IP value evaluation, IP management, global IP management, IP commercialization, and IP disputes, of which validity was verified. Second, It was analyzed that the validity of required competencies derived from each field of intellectual property was reliable. Third, it is generally appropriate to nurture manpower in each field of intellectual property in universities or graduate schools. In addition, it is judged that regular job training of industries such as enterprises and public institutions is necessary regardless of the field.

Germination Continuity and Restoration of Salicornia europaea, Halophyte in West-coast of Korea (서해안 염생식물 퉁퉁마디의 발아지속과 복원)

  • Kim, Ki-Hoon;Kang, Nae-Kyu;Song, Uh-Ram;Lee, Eun-Ju
    • Journal of Environmental Science International
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    • v.21 no.2
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    • pp.181-188
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    • 2012
  • Salicornia europaea (glasswort) is succulent, annual, halophytic plant mainly distributed throughout reclaimed land or salt marsh. It has strong tolerance to salt so that it plays the part of the pioneer species in the first succession. According to domestic and foreign studies, S. europaea contains plenty of minerals and antioxidant in the body. Since people take note of an availableness of this plant as health diet, the natural growth sites are threatened. In addition to development of salt marsh and sea shore, imprudent harvest has a bad effect to S. europaea population maintenance. To seek ways to preserve the population of this plant, we carried out the continuity of seed germination and restoration test. Seokmo Island, Daebu Island, Youngjong Island and Sudokwon landfill in Korea are selected sites for research. Result of germination continuity shows that most S. europaea seeds germinate on March but no more after July. However the germination was occurred after that time in the greenhouse. So we concluded that no germination after July is a matter of environmental condition not the number of remaining seeds. Also germination was seldom occurred in the spot where seeds production was not happened. In result of continuity test of seed germination by soil depth, germination was occurred vigorously only in top soil. From these results, we note that most S. europaea germinate in the early spring, and germination is finished by July. And this rapid germination speed makes the number of seeds in soil seed bank rare. If a large number of S. europaea in some area are harvested after July, the number of this species will dramatically decrease in that area the following year. In Seokmo Island, we carried out reintroduction experiment by sowing S. europaea seeds. On the first year, a small number of S. europaea settled and they produced seeds successfully. On April 2010, the second year, we observed many S. europaea in seed sowing sites. And we found out that plowing is more efficient than treatment sea water for settlement of S. europaea.

Emerging Technologies in Mobile Communications for 2020 (2020년 미래 무선통신 유망기술 발굴)

  • Lee, Kyungpyo;Song, Youngkeun;Han, Woori;Lee, Sungjoo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38A no.1
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    • pp.108-126
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    • 2013
  • Recently, it becomes essential for firms or nations to forecast the future and identify emerging technologies in order to improve R&D efficiency and gain a competitive advantage. Particularly, the mobile communications industry is characterized by rapid advance and wide application of its technology and thus identifying emerging technologies is more important in the industry than in others. Nevertheless, few attempts have been made to explore its emerging technologies. Therefore, this research aims to develop a methodology to identify the future and emerging technologies especially for the industry and applied it to list top ten emerging technologies for 2020 in the industry. For this purpose, firstly, we focused the key issues in the future targeting 2020 and identified user needs relating to them. Then, candidates of emerging technologies were defined from a set of technologies to meet the needs, for which technological and economic feasibility is assessed to determine their priorities. Finally, the top ten most important technologies were selected and verified. This research is distinct from the previous studies in that it takes a market-pull approach instead of a technology-push approach. The research results are expected to provide valuable information to support strategy- and policy-makings in the mobile communications industry.

Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization

  • Lee, Won Young;Lee, Ran;Kim, Hee Chan;Lee, Kyung Hoon;Cui, Xiang Shun;Kim, Nam Hyung;Kim, Sang Hyun;Lee, Il Joo;Uhm, Sang Jun;Yoon, Min Jung;Song, Hyuk
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.10
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    • pp.1417-1425
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    • 2014
  • The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of semen mainly include total sperm motility, concentration, and morphology. However, these primary parameters are often not reliable for discriminating between normal and abnormal, non-fertilizable spermatozoa. The present study was designed to compare the motion characteristics, fertilization ability using in vitro fertilization (IVF), and acrosome formation of the semen from boars having low (boar number 2012) and normal (boar number 2004 and 2023) breeding performances. The ultimate goal was to identify additional simple and easy criteria for the selection of normal sperm. There was no significant difference between boar 2004 and boar 2023 sperm total motility in computer assisted sperm analysis. However, boar number 2012 semen presented a significantly reduced population of rapid moving spermatozoa and an increased population of slow moving spermatozoa compared to boar numbers 2004 and 2023. Analysis of detailed motion characteristics revealed that sperm from boar number 2012 had significantly reduced motility in progressiveness, average path velocity, straight-line velocity (VSL), curvilinear velocity (VCL), straightness, and linearity. The assessment of the fertilizing ability by IVF also showed that sperm from boar number 2012 showed a fertility rate of 3.4%, whereas sperm from boar number 2023 had a fertility rate of 75.45%. Interestingly, most of the sperm nuclei were found on the peripheral area of the oocytes, suggesting that the sperm from boar number 2012 lacked penetration ability into the oocyte zonapellucida. The acrosome formation analysis using Pisum sativum agglutinin staining demonstrated that the sperm from boar number 2012 had a defect in acrosome formation. Consequently, primary parameters for selecting semen before AI such as motility are not sufficient to select normal and fertilizable spermatozoa. In conclusion, the present study suggests that the acrosome staining and detailed motion characteristics such as progressiveness, VCL, and VSL should be included in determining semen quality together with primary parameters for successful AI and high breeding performance in the swine industry.