• Applied Biological Chemistry
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• v.40 no.3
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• pp.238-242
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• 1997

Seeds of five radish varieties were soaked in water for three hours and cellulose was coated. The seeds were classified as three groups in UV light; non-fluorescent(NF), partly fluorescent(PF), and fully fluorescent(FF) seeds. Germination rate was less in the order of NF>PF>FF seeds. The seed coat structure of NF seeds was dense and showed round shape, while those of PF and FF seeds were wrinkled or ruptured. The cotyledon and hypocotyl of NF seeds were normal, while those of PF and FF seeds were dwarf and showed some scars in the cotyledons.

• Korean Journal of Pharmacognosy
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• v.35 no.3 s.138
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• pp.250-254
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• 2004

Helicobacter pylori (H. pyroli) infection it associated with type B gastritis, peptic uler disease, and gastric cancer. The vacuolation of cells induced by H. pylori is thought to be essential for the initiation and maintenance of gastric infection. The roles of H. pylori cytotoxin, urease, and ammonia in the vacuolation of HeLa cells were determined. H. pylori toxin induced vacuolation of HeLa cells. Korean and Japanese radishes significantly prevented the vacuolation of HeLa cells induced by H. pylori toxin. The urease activity in vacuolated cells was also decreased with Korean and Japanese radishes. H. Pylori toxin-induced vacuolation was inhibited by vacuolar type ATPase inhibitors (bafilomycin and N-ethylmaleimide). However, further investigation is required to determine the mechanisms of radish for the inhibition of vacuole formation of eukaryotic cells in response to the H. pylori toxin.

• Korean journal of food and cookery science
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• v.31 no.5
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• pp.596-604
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• 2015

This study investigated changes in the chemical properties and antioxidant activity of dried shredded radish (Raphanus sativus L.). Shredded radishes with a thickness of either 3.5 mm or 7.0 mm were dried using hot air at $45^{\circ}C$ or were sun dried. The degree of browning for the 3.5 mm sun dried radish, the 7.0 mm sun dried radish, the 3.5 mm hot air dried radish and the 7.0 mm hot air dried radish was 0.12, 0.14, 0.04 and 0.04, respectively. The DPPH radical scavenging activity (EDA%) of the hot air dried radish had a higher value than the sun dried radish. In contrast, the ABTS values of the sun dried radishes were higher than the hot air dried radishes. The total polyphenol content of the 7.0 mm and 3.5 mm hot air dried radishes reached high values of 15.99 and 11.62 mg/g, respectively. The total polyphenol contents of the 7.0 mm and 3.5 mm sun dried radishes were 9.63 and 6.77 mg/g, respectively. In sensory evaluation of the stir-fry dried shredded radishes, the 7.0 mm hot air dried sample scored the highest in terms of smell, color, gloss and overall preference. In conclusion, hot air drying ($45^{\circ}C$) and a thickness of 7.0 mm were found to be the optimal conditions for dried shredded radish and stir-fried dried shredded radish products.

• The Journal of Natural Sciences
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• v.5 no.1
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• pp.29-36
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• 1992

The experiment was conducted to evaluate the effects of organic fertilizer. Sarang of fermented poultry manure. Miwon glutamic acid fertilizer and poultry manure, on the growth and yield of radish cultivated in spring and autumn. 1) The growth of leaf length, leaf weight, root length, root diameter and root weight of radish in two culture types was stimulated in order of NPK, fermented poultry manure + 1/5NK, fermented poultry manure, poultry manure and control treatment, and chlorophyll and nitrogen content of leaf were increased in order of the above mentioned fertilizers.2) Yield decreased in fermented poultry manure plot of 450kg application comparison with NPK plot but the one of 1800kg fermented poultry manure was increased. The yield of 450kg Sarang plot was sufficient as compared with Korean average yield of spring and autumn culture.3) The difference of growth of radish between treatments of fermented poultry manure and NPK was lowered by continuous application of Sarang organic fertilizer.4) Soil nitrogen content and exchangeable Mg were similar in all treatments but soil pH, the contents of organic matter, $P_2 O_5$ and Ca were high in the fermented poultry manure treatment.

• The Journal of Natural Sciences
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• v.4
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• pp.179-189
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• 1991

The limit sowing time and the growth of radish cultivated in late fall were studied in relation to the effects of varieties and polyethylene film mulching. The root weight of Dehyung-Chusuk radish sowing in September 8th in Suwon was 520g but 718g in mulched plot. In Pusan the root weight of radish sowing on September 16th and harvested on November 23rd was 655g in Bekkyung and 813g in Dehyung-Chusuk variety, but more than 1000g in mulched plots of two varieties all. The effect of mulching was higher in Dehyung-Chusuk than in Bekkyung. The experimental results of Altari varieties will be recommandable in limit sowing time as around September 14th in mid-northern area and around September 18th in mid-southern area. Chonggak Altari was better than Chammat Altari in root formatiion. Two varieties all revealed good growth in the plots of sowing on September 26th in Pusan.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.397-402
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• 2009

Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.

• Journal of Dairy Science and Biotechnology
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• v.40 no.3
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• pp.122-133
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• 2022

Radish (Raphanus sativus L.) has long been recognized as a source of nutrients and phytochemicals. It is well known that the antioxidant properties of radish contributed to its popularity in pharmaceuticals. For this reason, the radish extract aided in the recovery and prevention from diseases. Hence, in this study, the sensory evaluation assessment was conducted following addition of radish oil to almond milk, oat milk, and soy milk (nondairy products) and Kefir, yogurt, and cow milk (dairy products) at different concentrations (containing 0.5% increments from 0% up to 2%). According to the results obtained in this study, all samples containing 0.5% radish oil scored higher on the sensory evaluation scale than the control. It is also very valuable as it is the first study to investigate sensory evaluation assessment by incorporating radish oil into almond milk, oat milk, and soy milk (nondairy products) and kefir, yogurt, and cow milk (dairy products). Furthermore, the results of this study can be used as a foundation for developing future products using radish oil. Additional research on addition of plant-based essential oil to various foods should be conducted.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.108-115
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• 2001

For determining the effect of extracts exudated from ten industrial plant resources on germination and early growth of shoot and root of radish the allelopathic compounds screened. Germination rate and seedling growth of radish differed depending on plant species and extract concentration. Lower levels of 1% extracts exudated from industrial plant resources promoted the rate of germination of radish while higher levels of 10% extract inhibited the rate of germination. Germination rate, shoot growth and root growth of radish in higher concentration of extract also differed depending on the plant species exudated and extract concentraton of industrial plant resources. Higher concentration of extracts from Xanthium strutnarium L. Angellica gigas Nakai, and Solanum nigrum L. were completely inhibited the germination while those from Cichorium intybus L., and Polygonatum officinale Allioni were not inhibited. Extracts exudated from Xanthium strutnarium L, Angellica gigas Nakai, and Solanum nigrum L. showed the strong inhibition of shoot and root growth of radish while those from Cichorium intybus L., and Polygonatum officinale Allioni did not show the bioactivity in shoot and root growth of radish..

• Horticultural Science & Technology
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• v.35 no.2
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• pp.210-219
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• 2017

This study was conducted to establish an efficient screening method for radish (Raphanus sativus) cultivars that are resistant to black spot, which is caused by Alternaria brassicicola. Seven A. brassicicola isolates were selected and investigated for their ability to produce spores and pathogenicity. Of these isolates, A. brassicicola KACC 40036 and 43923 produced abundant spores in V-8 juice agar medium and showed pathogenicity and strong virulence on radish seedlings. We examined the resistance of 61 commercial cultivars of radish to A. brassicicola KACC40036, and found that there are no highly resistant radish cultivars; however, some cultivars, such as 'Geumbong' and 'Searom', showed weak resistance to A. brassicicola. For further study, we selected four radish cultivars that showed different disease responses to A. brassicicola KACC40036. According to the growth stage of the radish seedlings, inoculum concentration, and incubation temperature of radish, development of black spot on four cultivars has been investigated. The results showed that younger seedlings were more sensitive to A. brassicicola than older seedlings, and the disease severity depended on the concentration of the spore suspension. The disease severity of plants incubated in humidity chamber at $25^{\circ}C$ was greater than that of plants grown at $20^{\circ}C$ or $30^{\circ}C$. Taken together, we suggest the following method for screening for radish plants that are resistant to A. brassicicola: 1) inoculate 16-day-old radish seedlings with an A. brassicicola spore suspension ($2.0{\times}10^5spores{\cdot}mL^{-1}$) using the spray method, 2) incubate the inoculated plants in a humidity chamber at $25^{\circ}C$ for 24 h and then transfer the plants to a growth chamber at $25^{\circ}C$ with 80% relative humidity under a 12 h light/dark cycle, and 3) assess the disease severity of the plants two days after inoculation.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.151-159
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• 2002

Genetic map and molecular marker have a great importance in improving and facilitating crop breeding program as well as in genome analysis and map-based cloning of genes representing desirable characters. This study aimed at developing RAPD markers and constructing a genetic linkage map using 82 BC$_1$F$_1$individuals originated from the cross between '835' and B$_2$in radish (Raphanus sativus L.). One of the parents for genetic linkage map construction, '835'(P$_1$) of egg type is susceptible to Fusarium wilt and have medium resistance to virus infection and the other parent, B$_2$(P$_2$) of round type, is susceptible to Fusarium wilt and virus, Screening of 394 RAPD primers in BC$_1$F$_1$) population resulted in selecting 128 polymorphic markers which displayed 1:1 segregation pattern. Two markers failed to display 1:1 segregation and showed the segregation ratio skewed to maternal genotype. Selected markers were categorized into 14 linkage group based on LOD score represented by MAPMAKER/EXP program. Five groups composed of single marker among them were excluded from the linkage map, and consequently, the remaining groups are well matched with the number of radish chromosome (n＝9). The linkage map constructed with 128 markers covers 1,688.3 cM and the average distance between markers was 13.8 cM. For developing STS marker, we determined the partial nucleotide sequence of OPE10 marker at both ends and designed a oligonucleotide primer pair based on this sequence. STS PCR using the primer pair displayed a single, clear band of which segregation is perfectly matched with that of OPE10 marker. This implies that RAPD markers could readily convert into clear and reliable STS markers.