• Title/Summary/Keyword: Raphanus

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Study of Distance Relationships among Domestic Radish (Raphanus sativus L.) by Analyzing its Anti-fungal Protein Gene. (항 곰팡이 단백질 유전자 분석에 의한 국내 무 품종간 유연성에 관한 연구)

  • Hwang, Cher-Won
    • Journal of Life Science
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    • v.17 no.9 s.89
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    • pp.1294-1297
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    • 2007
  • To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP[12]. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fun- gal, anti-yeast activity were done against Bot교tis cenerea, Saccharomyces cerevisiaeι Candida albicans with it's raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish's anti- fungal protein[15].

Organoleptic Characteristics of Dairy Products Supplemented with Raphanus raphanistrum subsp. sativus (radish) Powder: A Preliminary Study on Efficacy against Diabetes

  • Lim, Hyun-Woo;Song, Kwang-Young;Chon, Jung-Whan;Jeong, Dongkwan;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.37 no.3
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    • pp.177-186
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    • 2019
  • Raphanus raphanistrum subsp. sativus (radish) powder contains several bioactive com- pounds and is widely used in the food industry. In this study, we examined the sensory attributes (taste, color, flavor, texture, and overall acceptability) of commercially available milk, yoghurt, and kefir containing different concentrations of R. raphanistrum subsp. sativus powder. The organoleptic characteristics of commercially available milk, yoghurt, and kefir was significantly different with respect to taste, flavor, color, texture, and overall acceptability in the treated group (1%, 2%. 3%. and 4%) compared to the control (0%; p<0.05). In addition, the scores of taste, flavor, color, texture, and overall acceptability decreased in proportion to the increase in amount of radish-powder in commercially available milk, yoghurt, and kefir. When 1% R. raphanistrum subsp. sativus powder was added to commercially available milk, yoghurt, and kefir, they showed good results in organoleptic characteristics compared to the control group. Therefore, our results could be used as the basis for estimating changes in organoleptic characteristics on supplementation of various dairy products with R. raphanistrum subsp. sativus.

Toxicity Assessment of Nanopariticles Based on Seed Germination and Germination Index (씨앗발아 및 발아지수에 근거한 나노입자 독성평가)

  • Gu, Bonwoo;Kong, In Chul
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.6
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    • pp.396-401
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    • 2014
  • Nanomaterials have been widely used in many fields. This study investigates the effects of metal oxide nanoparticles (CuO, NiO, $TiO_2$, $Fe_2O_3$, $Co_3O_4$, ZnO) on germination and germination index (G.I.) of seeds, Lactuca and Raphanus. Under aqueous exposure, CuO on Lactuca shows the most significant impacts on activities compared to others, showing $EC_{50s}$ for germination and G.I. as 0.46 mg/L and 0.37%, respectively. The effects of nanoparticle phytotoxicity on seed Lactuca was much higher than that of Raphanus. In general, the toxicities on seed germination and germination index were as following orders : CuO > ZnO > NiO ${\gg}$ $TiO_2$, $Fe_2O_3$, $Co_3O_4$. No measurable inhibition was observed at 1,000 mg/L (maximum exposure concentration) of $TiO_2$, $Fe_2O_3$, $Co_3O_4$.

Radish (Raphanus sativus L. leaf) ethanol extract inhibits protein and mRNA expression of $ErbB_2$ and $ErbB_3$ in MDA-MB-231 human breast cancer cells

  • Kim, Woo-Kyoung;Kim, Ji-Hae;Jeong, Da-Hee;Chun, Young-Hee;Kim, Sun-Hee;Cho, Kang-Jin;Chang, Moon-Jeong
    • Nutrition Research and Practice
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    • v.5 no.4
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    • pp.288-293
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    • 2011
  • In this study, we investigated the effects of the ethanol extract of aerial parts of Raphanus sativus L. (ERL) on breast cancer cell proliferation and gene expression associated with cell proliferation and apoptosis in MDA-MB-231 human breast cancer cells. The MDA-MB-231 cells were cultured in the presence or absence of various concentrations (100, 200, or 300 ${\mu}g$/mL) of ERL. ERL significantly decreased cell proliferation after 48 h of incubation (P < 0.05). The protein and mRNA expression of $ErbB_2$ were decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of $ErbB_3$ was decreased significantly at an ERL concentration of 300 ${\mu}g$/mL (P < 0.05), and mRNA expression of $ErbB_3$ was decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of Akt was decreased significantly at the ERL concentration of 200 ${\mu}g$/mL (P < 0.05), and the protein expression of pAkt was decreased significantly in a dose-dependent manner (P < 0.05). The mRNA expression of Akt was decreased significantly at the ERL concentration of 200 ${\mu}g$/mL ERL (P < 0.05). The protein and mRNA expression of Bax were increased significantly at ERL concentrations of 200 ${\mu}g$/mL or higher (P < 0.05). The protein expression of $Bcl_2$ was increased significantly at ERL concentrations of 100 ${\mu}g$/mL or higher (P < 0.05), and mRNA expression of $Bcl_2$ was increased significantly at an ERL concentration of 300 ${\mu}g$/mL (P < 0.05). In conclusion, we suggest that Raphanus sativus, L. inhibits cell proliferation via the ErbB-Akt pathway in MDA-MB-231 cells.