Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Radish (Raphanus sativus L. leaf) ethanol extract inhibits protein and mRNA expression of $ErbB_2$ and $ErbB_3$ in MDA-MB-231 human breast cancer cells

  • Kim, Woo-Kyoung (Department Food Science and Nutrition, Dankook University) ;
  • Kim, Ji-Hae (Department Food Science and Nutrition, Dankook University) ;
  • Jeong, Da-Hee (Department of Food and Nutrition, Kookmin University) ;
  • Chun, Young-Hee (Department of Food and Nutrition, Kookmin University) ;
  • Kim, Sun-Hee (Department of Food and Nutrition, Kookmin University) ;
  • Cho, Kang-Jin (Functional Food and Nutrition Division, Rural National Academy of Agricultural Science) ;
  • Chang, Moon-Jeong (Department of Food and Nutrition, Kookmin University)
  • Received : 2011.04.19
  • Accepted : 2011.06.13
  • Published : 2011.08.31
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Abstract

In this study, we investigated the effects of the ethanol extract of aerial parts of Raphanus sativus L. (ERL) on breast cancer cell proliferation and gene expression associated with cell proliferation and apoptosis in MDA-MB-231 human breast cancer cells. The MDA-MB-231 cells were cultured in the presence or absence of various concentrations (100, 200, or 300 ${\mu}g$/mL) of ERL. ERL significantly decreased cell proliferation after 48 h of incubation (P < 0.05). The protein and mRNA expression of $ErbB_2$ were decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of $ErbB_3$ was decreased significantly at an ERL concentration of 300 ${\mu}g$/mL (P < 0.05), and mRNA expression of $ErbB_3$ was decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of Akt was decreased significantly at the ERL concentration of 200 ${\mu}g$/mL (P < 0.05), and the protein expression of pAkt was decreased significantly in a dose-dependent manner (P < 0.05). The mRNA expression of Akt was decreased significantly at the ERL concentration of 200 ${\mu}g$/mL ERL (P < 0.05). The protein and mRNA expression of Bax were increased significantly at ERL concentrations of 200 ${\mu}g$/mL or higher (P < 0.05). The protein expression of $Bcl_2$ was increased significantly at ERL concentrations of 100 ${\mu}g$/mL or higher (P < 0.05), and mRNA expression of $Bcl_2$ was increased significantly at an ERL concentration of 300 ${\mu}g$/mL (P < 0.05). In conclusion, we suggest that Raphanus sativus, L. inhibits cell proliferation via the ErbB-Akt pathway in MDA-MB-231 cells.

Keywords

References

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