• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.876-887
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• 2015

Dicer, an ribonuclease type III type endonuclease, is the key enzyme involved in biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs), and thus plays a critical role in RNA interference through post transcriptional regulation of gene expression. This enzyme has not been well studied in the Indian water buffalo, an important species known for disease resistance and high milk production. In this study, the primary coding sequence (5,778 bp) of bubaline dicer (GenBank: AB969677.1) was determined and the bubaline Dicer1 biocomputationally characterized to determine the phylogenetic signature among higher eukaryotes. The evolutionary tree revealed that all the transcript variants of Dicer1 belonging to a specific species were within the same node and the sequences belonging to primates, rodents and lagomorphs, avians and reptiles formed independent clusters. The bubaline dicer1 is closely related to that of cattle and other ruminants and significantly divergent from dicer of lower species such as tapeworm, sea urchin and fruit fly. Evolutionary divergence analysis conducted using MEGA6 software indicated that dicer has undergone purifying selection over the time. Seventeen divergent sequences, representing each of the families/taxa were selected to study the specific regions of positive vis-$\grave{a}$-vis negative selection using different models like single likelihood ancestor counting, fixed effects likelihood, and random effects likelihood of Datamonkey server. Comparative analysis of the domain structure revealed that Dicer1 is conserved across mammalian species while variation both in terms of length of Dicer enzyme and presence or absence of domain is evident in the lower organisms.

• Bulletin of the Korean Mathematical Society
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• v.53 no.5
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• pp.1531-1548
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• 2016

Let C[0, t] denote the space of real-valued continuous functions on [0, t] and define a random vector $Z_n:C[0,t]{\rightarrow}\mathbb{R}^n$ by $Z_n(x)=(\int_{0}^{t_1}h(s)dx(s),{\ldots},\int_{0}^{t_n}h(s)dx(s))$, where 0 < $t_1$ < ${\cdots}$ < $ t_n=t$ is a partition of [0, t] and $h{\in}L_2[0,t]$ with $h{\neq}0$ a.e. Using a simple formula for a conditional expectation on C[0, t] with $Z_n$, we evaluate a generalized analytic conditional Wiener integral of the function $G_r(x)=F(x){\Psi}(\int_{0}^{t}v_1(s)dx(s),{\ldots},\int_{0}^{t}v_r(s)dx(s))$ for F in a Banach algebra and for ${\Psi}=f+{\phi}$ which need not be bounded or continuous, where $f{\in}L_p(\mathbb{R}^r)(1{\leq}p{\leq}{\infty})$, {$v_1,{\ldots},v_r$} is an orthonormal subset of $L_2[0,t]$ and ${\phi}$ is the Fourier transform of a measure of bounded variation over $\mathbb{R}^r$. Finally we establish various change of scale transformations for the generalized analytic conditional Wiener integrals of $G_r$ with the conditioning function $Z_n$.

• Journal of Navigation and Port Research
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• v.34 no.6
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• pp.435-440
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• 2010

A numerical model, which can compute deformation of rubble mound structures composed with various size materials, was proposed. In the numerical model, wave field into the mound structures was computed by CADMAS-SURF and the deformations of mound structures were computed by DEM. Interaction between wave field and sectional deformation of structure was considered and to present the variation of behaviors caused by various properties of materials, computation was carried out with random coefficients by Monte Carlo simulation method for contact stiffness and friction angle. The experiments were carried out with rubbles and glass balls with radius of 2.9cm, 2.6cm and 1.5cm. And the deformation characteristics of rubble mounds composed with various size materials were clarified. Furthermore the validity and the applicability of the model were discussed by comparing with the experimental results.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.691-697
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• 2016

Remanufacturing refers to restoring a used product to an acceptable condition for resale in the market of remanufactured items. In this paper, we deal with the acquisition price and remanufacturing decision for remanufacturing systems in the case where the demand for the remanufactured product in a single period is known and the return quantity of the used product is determined by its acquisition price. The quality of the acquired used product is categorized into two classes, high and low, through inspection and different qualities incur different remanufacturing costs. The probability that the acquired used product is categorized as high class can be a constant or random variable. We derive the expected total cost functions, obtain the optimal solutions, and interpret the managerial meaning of the optimal solution for each case. The sensitivity of the optimal solution with respect to the variation of the inspection cost and uncertainty of the quality of the used product is investigated through numerical examples.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.3
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• pp.59-67
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• 1993

The variations of the natural frequencies and the peak response acceleration at the top of prestressed concrete reactor building due to random variability and/or model uncertainty of structural parameters are studied. The results may be used as essential input parameters in seismic probabilistic risk assessment or seismic margin assessment of the reactor building. The sensitivity test of each structural parameter is first performed to determine the most influential parameter upon the natural frequency of structure model. Then Monte Carlo simulation technique is applied to evaluate the effect of parameter variation on the natural frequencies and the peak response acceleration. The acceleration time history is obtained by direct integration scheme. As the study results, it is found that the fundamental natural frequency and the peak response acceleration at the top of the building are most strongly affected by Young's modulus among the structural parameters, in which the value of mean plus one standard deviation obtained by probabilistic approach deviates up to about (+)12% from the result of deterministic method. Considering the uncertainty of flexural rigidity, the structural responses vary in range of (-)4%~(+)14%.

• Fisheries and Aquatic Sciences
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• v.6 no.1
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• pp.13-19
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• 2003

Genomic DNA from the muscle of marsh clam (Corbicula leana) from Gochang, Muan and a Chinese site was extracted to identify genetic differences and similarity by randomly amplified polymorphic DNAs-polymerase chain reaction (RAPD- PCR). Out of 20 primers, seven primers produced amplified fragments which were consistently polymorphic. A total of 1,246 amplified products were produced of which 530 were polymorphic $(42.5\%)$. The number of polymorphic bands produced per primer varied from 40 to 122 with an average of 75.7 in marsh clam from Gochang. 3.28 of the 23.0 polymorphic bands per lane were found to be polymorphic. Also, about $4.34\%$ of total polymorphic bands were specific to marsh clam from Gochang. The major common bands of 0.28 kb generated by primer OPB-15 (GGAGGGTGTT) were present in every individuals, which were polymorphic. This common bands in every individuals should be diagnostic of specific strains, species and/or their relatedness. Primer OPB-19 (ACCCCCGAAG) produced the highest number of 12 specific bands. The intra-population variation was revealed in the band patterns identified by this primer. The random primer OPB-12 (CCTTGACGCA) yielded the amplified fragments which were consistently polymorphic between the marsh clams from Gochang and from Muan. This primer produced a total of 77 polymorphic bands: 31 bands from Gochang, 14 from Muan and 32 from the Chinese populations. An average of polymorphic bands were 1.8 from Gochang and 2.5 from the Chinese populations. This value obtained from the Chinese population was higher than those from the two domestic populations. Generally, the RAPD polymorphism generated by these primers may be useful as a genetic marker for strain or population identification of marsh clam.

• Journal of the Korean Society of Hazard Mitigation
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• v.7 no.2 s.25
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• pp.1-6
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• 2007

When the water content within concrete swells, diverse problems occur such as drop in durability. Due to this reason, a change is being managed in the unit water contents by using electric capacity measurements, high frequency heating methods, and unit-volume mass measurements, which are methods of measuring the unit water contents. Particularly, Japan is prescribing the guideline of management on a change in unit water content unit quantity $({\pm}10,\;15,\;20kg//m^3,\;etc.)$. However, the guideline of Japan dose not consider a fall in durability, and is decided on the value of pass-fail criteria by random fabrication errors and measurement errors. Consequently, this study was aimed to investigate the influence of a change In water content within concrete due to an addend caused by management error and to an artificial addend, upon drop in durability.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.2
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• pp.76-85
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• 2007

The total number of clinical materials collected from Dec 1, 2005 to Nov 30, 2006 in C. hospital was 63,133. Among these materials, the isolation of Staphylococcus spp. was 5,252 strains. The proportions of Staphylococcus species infection were as follows ; 46.7% in S. aureus, 39.3% in S. epidermidis, 4.7% in S. hominis, 3.8% in S. haemolyticus, 2.9% in S. capitis and 2.5% in others. Seventeen strains of Staphylococcus species were identified with an isolation range of 0.02-46.7%. The isolation ratios of Staphylococcus species were 7.8% (2,134/27,362) in female, 8.7% (3,118/35,807) in male and 8.3% (5,252/63,133) in both. The age-related frequencies of Staphylococcus species were 22.1% in the 60-69 year group, 19.3% in the 70-79 year group and 17.1% in the 50-59 year group. The isolation ratio of the 0-49 year group to the 50-over 80 year group were 1.74 times in female, 1.92 in male and 1.85 in both. The isolation frequency of Staphylococcus spp. was at its highest in April (9.9%) and at its lowest in February (6.8%) according to monthly analysis. According to seasonal variation, the isolation frequencies of Staphylococcus spp. were 27.0% in Summer, 25.9% in Spring, 23.9% in Fall and 23.2% in Winter. The clinical departments showing over 9% isolation rate against Staphylococcus spp. were critical care medicine (12.9%), department of pediatrics (11.6%), department of urology (9.8%) and department of neuro-surgery (9.1%). On the other hand, the lowest number was observed in the department of family medicine. The clinical material showing over 15% isolation rate against Staphylococcus spp. were 32.1% in urine (random urine and catheterized urine), 20.6% in blood and 16.0% in sputum.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.167-167
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• 2011

Magnetic random access memory (MRAM) is one of the prospective semiconductor memories for next generation. It has the excellent features including nonvolatility, fast access time, unlimited read/write endurance, low operating voltage, and high storage density. MRAM consists of magnetic tunnel junction (MTJ) stack and complementary metal-oxide semiconductor (CMOS). The MTJ stack is composed of various magnetic materials, metals, and a tunneling barrier layer. For the successful realization of high density MRAM, the etching process of magnetic materials should be developed. Among various magnetic materials, FePt has been used for pinned layer of MTJ stack. The previous etch study of FePt magnetic thin films was carried out using $CH_4/O_2/NH_3$. It reported only the etch characteristics with respect to the variation of RF bias powers. In this study, the etch characteristics of FePt thin films have been investigated using an inductively coupled plasma reactive ion etcher in various etch chemistries containing $CH_4$/Ar and $CH_4/O_2/Ar$ gas mixes. TiN thin film was employed as a hard mask. FePt thin films are etched by varying the gas concentration. The etch characteristics have been investigated in terms of etch rate, etch selectivity and etch profile. Furthermore, x-ray photoelectron spectroscopy is applied to elucidate the etch mechanism of FePt thin films in $CH_4$/Ar and $CH_4/O_2/Ar$ chemistries.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.1
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• pp.32-39
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• 2018

Repairing timing and the extended service life with repairing are very important for cost estimation during operation. Conventionally used model for repair cost shows a step-shaped cost elevation without consideration of variability of extended service life due to repairing. In the work, RC(Reinforced Concrete) Column is considered for probabilistic evaluation of repairing number and cost. Two mix proportions are prepared and chloride behavior is evaluated with quantitative exterior conditions. The repairing frequency and cost are investigated with varying service life and the extended service life with repairing which were derived from the chloride behavior analysis. The effect of COV(Coefficient of Variation) on repairing frequency is small but the 1st repairing timing is shown to be major parameter. The probabilistic model for repairing cost is capable of reducing the number of repairing with changing the intended service life unlike deterministic model of repairing cost since it can provide continuous repair cost with time.