• Korean Journal of Environment and Ecology
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• v.17 no.2
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• pp.169-176
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• 2003

The genetic relationships between 4 Korean native Taraxacum and 2 naturalized Taraxacum species were analyzed using the random amplified polymorphic DNA (RAPD) method. Because 141 polymorphic bands were generated from 30 random primers selected through the primer screening, it was possible to analyze the genetic relationship among 6 Taraxacum species. In RAED with the primer OPC12, OPD16, OPK16, OPK17, OPK20, OPS1 or OPS8, many specific polymorphic bands have been appeared in each species. Especially RAPD with the primer OPS8, a specific polymorphic band at 564bp was appeared only in the naturalized Taraxacum officinale. Based on RAPD analysis, Korean native Taraxacum and naturalized Taraxacum species are divided into two groups. T. officinale and T. laevigatum are classified into group I which is a naturalized Taraxacum species group, and T. mongolicum, T. hallasanensis, T. ohwianum and T. coreanum are classified into group II which is a Korean native Taraxacum species group. The result from the RAPD method was very similar to the result from the Bootstrap method. From the examination of the physical characteristics of 6 Taraxacum species populated in Korea, flowering period of Taraxacum species in group I are longer than Taraxacum species in group ll, and the direction of involucral bract of Taruxacum species in the group I was also different comparing to the group ll. Because the flowering color, leaf direction, and the specificity of seed germination of T. coreanum were different compared to the other species in the group II, T. coreanum would be genetically divergent and showed the highest dissimilarity index score.

• Journal of the Korean Applied Science and Technology
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• v.8 no.1
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• pp.27-34
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• 1991

동백종실유(冬栢種實油)에서 컬럼크로마토그래피로 지질(脂質) 성분(成分)을 상호(相互) 분리(分離)하였다. 여기서 얻은 트리글리세리드의 일부(一部)를 취하여 알칼리로 가수분해(加水分解)하거나 또는 pancreatic lipase로 가수분해(加水分解) 하여, 트리글리세리드의 구성(構成) 지방산(脂肪酸) 또는 1, 3- 위치(位置)와 2-위치(位置)에 급합(給合) 한 지방산(脂肪酸) 조성(組成)을 조사(調査)하였다. 나머지 트리글리세리드는 16% $AgNO_3$ TLC로 이중결합선(二重結合敾)별로 나누었으며, 이렇게 나누어진 분획(分劃)을 다시 HPLC로 PN별로 재분획(再分劃) 하였다. 여기서 얻어진 획분(劃分)들 중 PN이 같은 것은 모두 모아서 알칼리 가수분해(加水分解)로 PN의 총지방산(總脂肪酸) 조성(組成)을, pancreatic lipase로 1,3 위치(位置)에 결합선(結合敾)한 지방산(脂肪酸)의 조성(組成)을 조사(調査) 하였다. 여기서 얻어진 결과(結果)로 부터 구성(構成) 트리그리셀리드 분자종(分子種)을 산출(算出)하였더니 다음과 같은 결과(算出)를 얻었다. 1) 종실유(種實油)는 투명(透明)한 액체(液體)로 그 함량(含量)이 73.5%였으며, 그 중 트리글리세리드가 94.8%, 극성(極性) 지질(極性)이 2.0%, 탄화(炭化) 수소(水素)가 1.8%였다. 2) $AgNO_3$-TLC로 트리글리세리드를 분획(分劃)하였더니 5개의 획분(劃分)을 얻었으며, 대부분(大部分)의 트리글리세리드가 이중(二重) 결합선(結合敾) 3${\sim}$5개인 band 2와 band 3에 80% 이상 존재(存在)하였다. 3) $AgNO_3-TLC$에서 얻은 각 획분(劃分)은 모두가 HPLC상에서 PN 46, 48 및 50으로 나누어졌으며 전체(全體) 분획(分劃)에서 PN 48이 78.13%로 제일 많았으며, 다음으로 PN 50이 12.04%였으며, PN 46이 9.83%였다. 4) 전체(全體) 트리글리세리드 분자종(分子種) 중에서 0.1mol% 이상을 차지하고 있는 분자종(分子種)이 43종(種)이었으며, OOO와 POO가 각각 39.8mol%와 25.8mol%로 제일 많았으며, 그 다음으로 OPO가 5.5 mol%, OOL가 4.8 mol%, POS가 3.9mol%, SOO가 3.5mol%. POL이 3.0mol%였다. 또 동백종실유(冬栢種實油)에 존재(存在)하는 트리글리세리드의 분자종(分子種)의 조성(組成)은 1,3-random, 2-random 분포설(分布說)에 따라 구성(分布說)되어 있었다.

• Korean Journal of Optics and Photonics
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• v.31 no.5
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• pp.223-230
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• 2020

In this paper, we have studied the characteristics of stimulated Brillouin scattering (SBS) and mode instability (MI) in a ytterbium-doped polarization-maintaining fiber laser with master oscillator power amplifier configuration. We measured the laser output power and back-reflection spectrum for a variety of ytterbium-doped fibers and seed lights, to investigate the power-scaling limits of fiber lasers. By optimizing the laser structure, we demonstrated an all-fiber high-power polarization-maintaining fiber laser with near-diffraction-limited beam quality. The output power of 1.5 kW was achieved with a linewidth of 10 GHz, generated by pseudo-random binary sequence (PRBS) phase modulation. The beam quality M² was about 1.15 at the maximum output power. The polarization extinction ratio (PER) was greater than 17 dB.

• Journal of Internet Computing and Services
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• v.8 no.5
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• pp.141-150
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• 2007

This paper proposes new Steganographic model for VoIP that has very effective method using low bit encoding. Most of Steganographic models using Low bit Encoding have two disadvantages; one is that the existence of hidden secret message can be easily detected by auditory, the other is that the capacity of stego data is low. To solve these problems, this method embed more than one bit in inaudible range, so this method can improve the capacity of the hidden message in cover data. The embedding bit position is determined by using a pseudo random number generator which has seed with remaining message length, so it is hard to detect the stego data produced by the proposed method. This proposed model is able to use not only to communicate wave file with hidden message in VoIP environment but also to hide vary information which is user basic information, authentication system, etc.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.5
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• pp.419-422
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• 2004

Soybean is a major source of protein meal in the world. Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to identify RAPD markers linked to KTI protein allele using bulked segregant analysis. Cultivar Jinpumkong2 (TiTi) was crossed with C242 (titi, absence of KTI protein) and F. seeds were planted. The $F_1$. plants were grown in the greenhouse to produce $F_2$ seeds. Each $F_2$ seed from $F_1$. plants was analysed electrophoretically to determine the presence of the KTI protein band. The present and absent bulks contained twenty individuals each, which were selected on the basis of the KTI protein electrophoresis, respectively. Total 94 $F_2$ individuals were constructed and 1,000 Operon random primers were used to identify RAPD primers linked to the Ti locus. The presence of KTI protein is dominant to the lack of a KTI protein and Kunitz trypsin inhibit protein band is controlled by a single locus. Four RAPD primers (OPAC12, OPAR15, OPO12, and OPC08) were linked to the Ti locus. RAPD primer OPO12 was linked to Ti locus, controlling kunitz trypsin inhibitor protein at a distance of 16.0 cM. This results may assist in study of developing fine map including Ti locus in soybean.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.4 no.2 s.7
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• pp.53-66
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• 2005

Microscopic simulation models, which focus on individual vehicles, are used extensively in the transportation operations in order to capture vehicle to vehicle and vehicle to traffic controls. There are many important factors involved when a micro simulation model is applied as an evaluation tool for the traffic system. They typically include test bed, software, simulation time period, measure of effectiveness, parameter for a software, OD matrix, and random seed number. To evaluate the effects of those factors, sensitivity analysis was performed to identify the effects of those factors based on CORSIM and TRANSIMS results. Three freeway corridors, two in Houston (I-10 and US 290) and one in Sin Antonio (I-37), were chosen as test beds. Three time periods (AM, PM, off-peak) and three OD matrix estimates were used for the analysis. It was found that the micro-simulation results were highly sensitive to the model Parameter sets and the OD information. The degree of sensitivity was a function of the test bed and time period.

• Journal of Life Science
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• v.14 no.2
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• pp.345-350
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• 2004

The present study was investigated microgeographic variations to spatial autocorrelation in the Korean alder, Alnus hirsuta. Separate counts of each type of join (combination of genotypes at a single locus) for each allele, and for each distance class of separation, were tested for significant deviation from random expectations by calculating the Standard Normal Deviation (SND). Moran's I was significantly different from the expected value in 24 of 120 cases (20.0%). 17 of these values (14.2%) were significantly negative, indicating genetic dissimilarity among pairs of individuals in the ten distance classes. Many Korean populations of alder are small and are distributed by men for firewood. This occasional cutting of seed-bearing stems may bring a high level of gene flow. In addition, stump sprouting ability also may contribute to the fact that the Chengkwang population at Gijang is unusual in lacking spatial genetic structure.

• Korean Journal of Plant Tissue Culture
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• v.24 no.3
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• pp.175-181
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• 1997

Plants were regenerated from hypocotyl protoplast culture of cabbage (F$_1$hybrid Green Challenger) and were transplanted on fields. The ploidy level of regenerated and seed-grown plants was measured by flow cytometry. In total 125 regenerated plant, diploid (2n), tetraploid(4n), and mixoploid (2n+4n) were 72.8%, 25.6%, and 1.6%, respectively. Most of the regenerated plants had normal phenotype, whereas several plane showed abnormal phenotype such as heavy leaf incision, savoy, and wave. The regenerated plants with abnormal phenotype and different ploidy level were analysed by isozyme and RAPD, but no significant difference was found.

• Clinical Psychopharmacology and Neuroscience
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• v.16 no.4
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• pp.449-460
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• 2018

Objective: Prior functional magnetic resonance imaging (fMRI) work has revealed that children/adolescents with disruptive behavior disorders (DBDs) show dysfunctional reward/non-reward processing of non-social reinforcements in the context of instrumental learning tasks. Neural responsiveness to social reinforcements during instrumental learning, despite the importance of this for socialization, has not yet been previously investigated. Methods: Twenty-nine healthy children/adolescents and 19 children/adolescents with DBDs performed the fMRI social/non-social reinforcement learning task. Participants responded to random fractal image stimuli and received social and non-social rewards/non-rewards according to their accuracy. Results: Children/adolescents with DBDs showed significantly reduced responses within the caudate and posterior cingulate cortex (PCC) to non-social (financial) rewards and social non-rewards (the distress of others). Connectivity analyses revealed that children/adolescents with DBDs have decreased positive functional connectivity between the ventral striatum (VST) and the ventromedial prefrontal cortex (vmPFC) seeds and the lateral frontal cortex in response to reward relative to non-reward, irrespective of its sociality. In addition, they showed decreased positive connectivity between the vmPFC seed and the amygdala in response to non-reward relative to reward. Conclusion: These data indicate compromised reinforcement processing of both non-social rewards and social non-rewards in children/adolescents with DBDs within core regions for instrumental learning and reinforcement-based decision-making (caudate and PCC). In addition, children/adolescents with DBDs show dysfunctional interactions between the VST, vmPFC, and lateral frontal cortex in response to rewarded instrumental actions potentially reflecting disruptions in attention to rewarded stimuli.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.1
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• pp.1-11
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• 2023

The Indian golden muga silkworm, Antheraea assamensis Helfer is an economically important wild silkworm endemic to Northeastern part of India. In recent years, climate change has posed a threat to muga silk production due to the requirement that larvae be reared outdoors. Since the muga silkworm larvae are exposed to the vagaries of nature, the changing climate has increased the incidence of microbial diseases in the rearing fields. Accurate diagnosis of the disease causing pathogens and its associated epidemiology are prerequisites to manage the diseases in the rearing field. Although conventional microbial culturing methods are widely used to identify pathogenic bacteria, they would not provide meaningful information on a wide variety of silkworm pathogens. The information on use of molecular diagnostic tools in detection of microbial pathogens of wild silk moths is very limited. A wide range of molecular and immunodiagnostic techniques including denaturing gradient gel electrophoresis (DGGE), random amplified polymorphism (RAPD), 16S rRNA/ITSA gene sequencing, multiplex polymerase chain reaction (M-PCR), fluorescence in situ hybridization (FISH), immunofluorescence, and repetitive-element PCR (Rep-PCR), have been used for detecting and characterizing the pathogens of insects with economic significance. Nevertheless, the application of these molecular tools for detecting and typing entomopathogens in surveillance studies of muga silkworm rearing is very limited. Here, we discuss the possible application of these molecular techniques, their advantages and major limitations. These methods show promise in better management of diseases in muga ecosystem.