• Title/Summary/Keyword: Random Seed

Search Result 85, Processing Time 0.036 seconds

Classification of Capsicum annuum Germplasm Using Random Amplified Polymorphic DNA (RAPD를 이용한 고추(Capsicum annuum) 유전자원의 분류)

  • Nam, Seung-Hyun;Choi, Geun-Won;Yoo, Il-Woong
    • Horticultural Science & Technology
    • /
    • v.16 no.4
    • /
    • pp.503-507
    • /
    • 1998
  • This study was initiated to evaluate genetic relationship among various domestic and exotic pepper accessions using random amplified polymorphic DNA(RAPD) markers. The results suggested that the optimum conditions for PCR with random primers in Capsicum spp. could be obtained with 3mM of $MgCl_2$, 1.5U of Taq. DNA polymerase, 10ng of template DNA, $200{\mu}M$ of dNTPs, 200nM of random primer, and $42^{\circ}C$ of annealing temperature. Sixteen random primers showing high band intensity and reproducibility were selected from 80 random primers. Primers having 70% GC content were more effective in DNA amplification than primers having 60% GC content. The total 93 DNA bands including 71 polymorphic bands and 22 monomorphic bands were obtained with selected 16 random primers for 31 pepper cultivars and lines. About 4.4 polymorphic bands per primer were produced. Similarity coefficients were calculated by using 71 polymorphic bands and dendrogram based on the similarity coefficient showed clear classification of 31 peppers into three Capsicum species of Capsicum annuum, Capsicum chinense and Capsicum chacoense.

  • PDF

Ruthenium Thin Films Grown by Atomic Layer Deposition

  • Shin, Woong-Chul;Choi, Kyu-Jeong;Jung, Hyun-June;Yoon, Soon-Gil;Kim, Soo-Hyun
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 2008.11a
    • /
    • pp.12-12
    • /
    • 2008
  • Ruthenium is one of the noble metals having good thermal and chemical stability, low resistivity, and relatively high work function(4.71eV). Because of these good physical, chemical, and electrical properties, Ru thin films have been extensively studied for various applications in semiconductor devices such as gate electrode for FET, capacitor electrodes for dynamic random access memories(DRAMs) with high-k dielectrics such as $Ta_2O_5$ and (Ba,Sr)$TiO_3$, and capacitor electrode for ferroelectric random access memories(FRAMs) with Pb(Zr,Ti)$O_3$. Additionally, Ru thin films have been studied for copper(Cu) seed layers for Cu electrochemical plating(ECP) in metallization process because of its good adhesion to and immiscibility with Cu. We investigated Ru thin films by thermal ALD with various deposition parameters such as deposition temperature, oxygen flow rate, and source pulse time. Ru thin films were grown by ALD(Lucida D100, NCD Co.) using RuDi as precursor and $O_2$ gas as a reactant at 200~$350^{\circ}C$.

  • PDF

On the SEED Validation System (SEED 구현 적합성 검증 시스템에 관한 연구)

  • Kim, Yeok;Jung, Chang-Ho;Jang, Yoon-Seok; Lee, Sang-Jin;Lee, Sung-Jae
    • Journal of the Korea Institute of Information Security & Cryptology
    • /
    • v.13 no.1
    • /
    • pp.69-85
    • /
    • 2003
  • In this paper, we discuss a validation test for cryptographic algorithms. The cryptographic algorithms decide on the security and the confidence of a security system protecting sensitive information. So. the implementation of cryptographic algorithms is very critical of the system. The validation lest specifies the procedures involved in validating implementations of the cryptographic standards and provides conformance testing for components or procedures of the algorithm. We propose a SEED Validation System(SVS) to verify that the implementation correctly performs the SEED algorithm. The SVS is composed of two types of validation tests, the Known Answer test and the Monte Carlo test. The System generates the testing data for the Known Answer tests and the random data for the Monte Carlo tests. This system can be used to validate and certify the cryptographic product.

A Study on Video Data Protection Method based on MPEG using Dynamic Shuffling (동적 셔플링을 이용한 MPEG기반의 동영상 암호화 방법에 관한 연구)

  • Lee, Ji-Bum;Lee, Kyoung-Hak;Ko, Hyung-Hwa
    • Journal of Korea Multimedia Society
    • /
    • v.10 no.1
    • /
    • pp.58-65
    • /
    • 2007
  • This dissertation proposes digital video protection algorithm lot moving image based on MPEG. Shuffling-based encryption algorithms using a fixed random shuffling table are quite simple and effective but vulnerable to the chosen plaintext attack. To overcome this problem, it is necessary to change the key used for generation of the shuffling table. However, this may pose a significant burden on the security key management system. A better approach is to generate the shuffling table based on the local feature of an image. In order to withstand the chosen plaintext attack, at first, we propose a interleaving algorithm that is adaptive to the local feature of an image. Secondly, using the multiple shuffling method which is combined interleaving with existing random shuffling method, we encrypted the DPCM processed 8*8 blocks. Experimental results showed that the proposed algorithm needs only 10% time of SEED encryption algorithm and moreover there is no overhead bit. In video sequence encryption, multiple random shuffling algorithms are used to encrypt the DC and AC coefficients of intra frame, and motion vector encryption and macroblock shuffling are used to encrypt the intra-coded macroblock in predicted frame.

  • PDF

Cloning of CAB cDNA encoding chlorophyll a/b binding protein of photosystem II in Korean ginseng and Use in Plant (고려인삼 광계 II Chlorophyll a/b binding Protein 유전자(CAB)의 cloning 및 식물에의 활용연구)

  • 김갑식;이기원;이종철;여운형;채순용;박은경
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.21 no.2
    • /
    • pp.152-159
    • /
    • 1999
  • A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

  • PDF

Genetic Differences of Two Wild Shortnecked Clam(Ruditapes philippinarum) Populations from the Yellow Sea Analysed by Random Amplified Polymorphic DNAs-Polymerase Chain Reaction

  • Yoon, Jong-Man;Kim, Yong-Ho
    • Proceedings of the Korean Society of Fisheries Technology Conference
    • /
    • 2003.05a
    • /
    • pp.229-230
    • /
    • 2003
  • Shortnecked clam is a commercially important mollusks species, which is distributed all over the Yellow Sea. Consequent of the rapid increase in seed production, there is a need to understand the genetic composition of wild shortnecked clam populations in order to evaluate exactly the latent genetic effects induced by seed production operations. Many genetic and molecular researches were made because RAPD-PCR is a simple and rapid method for determining genetic diversity and similarity in various organisms with the advantage that no prior knowledge of the genome under research is needed (Fischer et at., 2000). (omitted)

  • PDF

Seed-dependent Accelerated Fibrillation of ${\alpha}$-Synuclein Induced by Periodic Ultrasonication Treatment

  • Kim, Hyun-Jin;Chatani, Eri;Goto, Yuji;Paik, Seung-R.
    • Journal of Microbiology and Biotechnology
    • /
    • v.17 no.12
    • /
    • pp.2027-2032
    • /
    • 2007
  • [ ${\alpha}$ ]-Synuclein is the major component of Lewy bodies and responsible for the amyloid deposits observed in Parkinson's disease. Ordered filamentous aggregate formation of the natively unfolded ${\alpha}$-synuclein was investigated in vitro with the periodic ultrasonication. The ultrasonication induced the fibrillation of ${\alpha}$-synuclein, as the random structure gradually converted into a ${\beta}$-sheet structure. The resulting fibrils obtained at the stationary phase appeared heterogeneous in their size distribution, with the average length and height of $0.28\;{\mu}m{\pm}0.21\;{\mu}m$ and $5.6\;nm{\pm}1.9\;nm$, respectively. After additional extensive ultrasonication in the absence of monomeric ${\alpha}$-synuclein, the equilibrium between the fibril formation and its breakdown shifted to the disintegration of the preexisting fibrils. The resulting fragments served as nucleation centers for the subsequent seed-dependent accelerated fibrillation under a quiescent incubation condition. This self-seeding amplification process depended on the seed formation and subsequent alterations in their properties by the ultrasonication to a state that accretes the monomeric soluble protein more effectively than their reassociation of the seeds back to the original fibrils. Since many neurodegenerative disorders have been considered to be propagated via the seed-dependent amyloidosis, this study would provide a novel aspect of the significance of the seed structure and its properties leading to the acce]erated amyloid formation.

Adaptive Random Testing for Integrated System based on Output Distribution Estimation (통합 시스템을 위한 출력 분포 기반 적응적 랜덤 테스팅)

  • Shin, Seung-Hun;Park, Seung-Kyu;Choi, Kyung-Hee;Jung, Ki-Hyun
    • Journal of the Korea Society for Simulation
    • /
    • v.20 no.3
    • /
    • pp.19-28
    • /
    • 2011
  • Adaptive Random Testing (ART) aims to enhance the performance of pure random testing by detecting failure region in a software. The ART algorithm generates effective test cases which requires less number of test cases than that of pure random testing. However, all ART algorithms currently proposed are designed for the tests of monolithic system or unit level. In case of integrated system tests, ART approaches do not achieve same performances as those of ARTs applied to the unit or monolithic system. In this paper, we propose an extended ART algorithm which can be applied to the integrated system testing environment without degradation of performance. The proposed approach investigates an input distribution of the unit under a test with limited number of seed input data and generates information to be used to resizing input domain partitions. The simulation results show that our approach in an integration environment could achieve similar level of performance as an ART is applied to a unit testing. Results also show resilient effectiveness for various failure rates.

PRIMORDIAL BLACKHOLE AS A SEED FOR THE COSMIC MAGNETIC FIELD

  • LA DAIL;PARK CHANGBOM
    • Journal of The Korean Astronomical Society
    • /
    • v.29 no.2
    • /
    • pp.83-91
    • /
    • 1996
  • We present a model that rotating primordial blackholes(PBHs) produced at the end of inflation generate the random, non-oriented primordial magnetic field. PBHs are copiously produced as the Universe completes the cosmic phase transition via bubble nucleation and tunneling processes in the extended inflation hypothesis. The PBHs produced acquire angular momentum through the mutual tidal gravitational interaction. For PBHs of mass less than 1013g, one can show that the evaporation (photon) luminosity of PBHs exceeds the Eddington limit. Thus throughout the lifetime of the rotating PBH, radiation flow from the central blackhole along the Kerr-geodesic exerts torque to ambient plasma. In the process similar to the Bierman's battery mechanism electron current reaching up to the horizon scale is induced. For PBHs of Grand Unified Theories extended inflation with the symmetry breaking temperature of $T_{GUT}\;\~\;10^{10}$ GeV, which evaporate near decoupling, we find that they generate random, non-oriented magnetic fields of $\~10^{-11}G$ on the last-scattering surface on (the present comoving) scales of $\~O(10)Mpc$.

  • PDF

Construction of a linkage Map in Capsicum annuum L. Using RAPD Markers and Identification of Two QTLs.

  • Yang, Tae-Jin;Kim, Yong-Jae;Park, Hyo-Guen
    • Journal of Plant Biotechnology
    • /
    • v.1 no.2
    • /
    • pp.109-115
    • /
    • 1999
  • A linkage map of Capsicum annuum L. was constructed by random amplified polymorphic DNA (RAPD) markers followed in a backcross population of an intraspecific cross between cultivars HDA210 and Yatsufusa. A total of 420 random primers were tested and 311 polymorphic bands were generated by 158 random primers. Among them, 86 Yatsufusa specific bands generated by 52 primers were examined for mapping. Most bands except three segregated in Mendelian fashion fitting the expected 1:1 ratio. The total length of the map was 533 cM distributed in 15 linkage groups. The map distance between adjacent markers ranged 0 to 32.8 cM, with an average distance of 9.1 cM (63 markers). Some markers were clustered and this may be due to the amplification of a repetitive sequence by the RAPDs. Primer pairs for a sequence characterized amplified region (SCAR) were developed and the segregation scores by the SCAR primers were in accordance with the RAPD data. Two QTL markers for number of axillary shoots and for early flowering were developed. One QTL for early flowering located in the linkage group 3 and explained 61 "io of the phenotypic variation. The other QTL for the number of axillary shoots located in the linkage group 4 explained 55 % of the phenotypic variation.tion.

  • PDF