• Korean Journal of Environmental Biology
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• v.21 no.1
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• pp.45-51
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• 2003

Genetic diversity of local populations among geologically isolated groups of Rana amurensis was refined by sequence comparison of the mitochondrial (mt) 165 rDNA genes. Each 401 base pairs of DNA sequences, which was determined from four local populations of Rana amurensis, two local populations of R. nigromacutata, and three species of the genus Rana were used in this analysis. Despite morphological similarity of Rana amurensis, Korean populations were well distinguished from the other groups on the basis of 105 rDNA gene difference. Further analyses for additional local populations belonging to R. amurensis will be necessary to clarify the taxonomic status.

• Animal cells and systems
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• v.3 no.3
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• pp.303-309
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• 1999

Inter- and intraspecific genetic relationships between Rana amurensis from Korea and Russia and other brown frogs were investigated by nucleotide sequence of a 504 base pair (bp) fragment of the mitochondrial cytochrome b gene. Nucleotide sequence similarities among Korean populations of R. amurensis ranged from 99.6% to 97.6% and 98.8% within Russian populations. The nucleotide sequence similarity between Korean and Russian R. amurensis ranged from 86.9% to 85.5%. Based on Kimura-2-parameter distance, the sequence divergence between R. amurensis from Korea and Russia was 16.18% and 18.04% among other related brown frogs. interspecific sequence divergences among R. amurensis and other related brown frogs diverged by 20.3%. Using an estimate of 2-4% mitochondrial DNA sequence divergence per million years, Korean and Russian R. amurensis diverged about 8 to 4 million years ago (Mya) and other brown frogs diverged about 9 to 5 Mya from ancestral frogs and distributed from North Asia to Sakhalin in a short time. In the neighbor-joining and UPGMA tree R. amurensis was clustered into two groups with Korean and Russian populations and the other brown frogs were grouped separately with diverged trichotomous clusters (R. dybowskii and R. pirica, R. okinavana and R. tsushimensis, and R. japonica and R. longicrus).

• The Korean Journal of Zoology
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• v.16 no.2
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• pp.109-118
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• 1973

The morphological changes of the enterochromaffin cells in the gastrointestinal mucosae of Rana amurensis were observed during pre-hibernating, hibernating, post-hibernating and active period. The results of the observation are as follow: 1. The relative values of the enterochromaffin cell numbers in the pre-hibernating, hibernating and post-hibernating groups as compared with the active group are 1.50, 0.36 and 0.90, respectively. 2. In active and pre-hibernating groups, the enterochromaffin cells are large and middle-amount granulated cells and the sparse granulated cells increase significantly during hibernating and post-hibernating periods. 3. It is concluded that the morphological changes of enterochromaffin cells are due to lowered metabolic acitvities especially lowered endocrine activity during hibernation.

• Animal cells and systems
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• v.4 no.1
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• pp.31-37
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• 2000

The genetic variations among six species of Rana from Korea (R. nigro-maculata, R. piancyi, R. dybowskii, R. sp, R. rugosa type A, B and R. amurensis) were investigated using 499 bases of mitochondrial DNA sequences for ND2 (NADH dehydrogenase subunit 2) gene and $tRNA^{Trp}$ gene. Partial sequences of ND2 gene (427 bp) and full sequences of $tRNA^{Trp}$ gene (73 bp) were identified. The level of sequence divergences ranged from 0.2 to 5.2% within species and 4.9-28.0% among 6 species of the genus Rana. The $tRNA^{Trp}$ gene of the genus Rana was composed of 77 nucleotides which showed a two dimensional "cloverleaf" structure. The secondary structure of $tRNA^{Trp}$ was not found compensatory changes which could potentially confound phylogenetic inference. In the neighborjoining tree, brown frogs were clustered first with the level of sequence divergence of 13.20% between R. amurensis and R. dybowskii, and 9% between R. dybowskii and R. sp. supported by 99% bootstrap iterations, respectively. R. nigromaculata and R. plancyi were clustered into another group with 5.1% divergence supported by 100% bootstrap iteration. R. rugosa A 8nd B types were grouped by 4.9% divergence and clustered into the last group with other two groups with 100% bootstrap iterations.

• Proceedings of the Zoological Society Korea Conference
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• 1998.04a
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• pp.41.1-41
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• 1998

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.249.1-249
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• 1996

No Abstract, See Full Text

• The Korean Journal of Zoology
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• v.36 no.1
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• pp.97-108
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• 1993

The biochemical characteristics of immunorecative atrial natriuretic peptide (irAUPP and the changes in the levels of irANP in the heart were investigated during the metamorphosis of frog tadpoles. Immunohistochemical localization of pro-ANP in cardiocfes and the presence of irAW in the peritoneal fluid of metamorphosing tadpoles were also examined. The major form of irANP in the cardiocvtes of tadpoles (Rana omurensisp was high molecular weight on gel filtration chromatographv and reverse-phase HPLC. The levels of irANP in the atrium of tadpoles were five to seven times higher than those in the ventricle. In metamorphosing tadpoles the levels of irANP in the atrium increased at stage XX, the climax of metamorphosis, and decreased at stage XXV (P < 0.051, the completion of metamorphosis. When the levels of irANP was expressed as a function of body weight of tadpoles, a continuous increase in the levels of irANP was observed from pre- to postmetamorphosis (P < 0.051. The levels of irAUP in the ventricle were found to be higher in the adult frogs than in tadpoles (R. omurensisl (P < 0.01). Pro-ANP (31-67) immunoreactivity was detected in the ventricle as well as in the atrium of tadpoles fR. nigromaculotal. The peritoneal fluid was also found to contain low molecular weight of irAUP and the levmils of irANP were 55.4 $\pm$ 9.1 pg/ml. Changes of the level of irANP at different stases of the life cycle suggest that ANP may play a role in the regulation of body fluid homeostasis of frog tadpoles during the metamorphosis.

• Animal cells and systems
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• v.4 no.2
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• pp.117-121
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• 2000

Genetic relationships among six species of the genus Rana from Korea were investigated by complete nucleotide sequence analyses of mitochondrial cytochrome b gene (1143 bp). Based on Kimura-2-parameter distance, the interspecific sequence differences of cytochrome b gene within the genus Rana were ranged from 7.83% to 25.00%. The genetic distances were 7.83% between R. nigromaculata and R. plancyi, 8.47% between two types of R. rugosa (type A and B), 10.42% between the brown frogs (R. amurensis and R. dybowskii), 16.11% between R. dybowskii types 1 and 2 and 12.36% between pond frogs (R. nigromaculata and R. plancyi) and R. catesbeiana. In the neighbor-joining and parsimony trees, R. catesbeiana was more closely related to pond frogs than brown frogs. R. dybowskii types 1 and 2 were considered to be at a distinct and specific level of differentiation (16.11%), while two types of R. rugosa were suspected to be at a subspecific level (8.47%).

• Journal of Biomedical Engineering Research
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• v.9 no.2
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• pp.239-246
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• 1988

This paper describes the design and implementation of personal computer assisted karyotyping system of Giemsa stained chromosomes. The system consists of an Image Acquisition Module being capable of $256 {\times} 256$ pixels and its relevent software modules optimized for karyotyping. The results of karyotyping using this system with an image of chromosomes taken from the Rana Amurensis are acceptable. As a result of this study we can save our load oweing to the conventional hand- karyotyping and the high-cost computer.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.44.1-44
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• 1999

No Abstract, See Full Text