Genetic Relationships among Six Korean Rana Species (Amphibia; Ranidae) Based on the Mitochondrial Cytochrome b Gene

  • Lee, Jung-Eun (Department of Biology, College of Natural Sciences, Inha University) ;
  • Yang, Suh-Yung (Department of Biology, College of Natural Sciences, Inha University) ;
  • Lee, Hei-Yung (Department of Biology, College of Natural Sciences, Inha University)
  • Published : 2000.06.01

Abstract

Genetic relationships among six species of the genus Rana from Korea were investigated by complete nucleotide sequence analyses of mitochondrial cytochrome b gene (1143 bp). Based on Kimura-2-parameter distance, the interspecific sequence differences of cytochrome b gene within the genus Rana were ranged from 7.83% to 25.00%. The genetic distances were 7.83% between R. nigromaculata and R. plancyi, 8.47% between two types of R. rugosa (type A and B), 10.42% between the brown frogs (R. amurensis and R. dybowskii), 16.11% between R. dybowskii types 1 and 2 and 12.36% between pond frogs (R. nigromaculata and R. plancyi) and R. catesbeiana. In the neighbor-joining and parsimony trees, R. catesbeiana was more closely related to pond frogs than brown frogs. R. dybowskii types 1 and 2 were considered to be at a distinct and specific level of differentiation (16.11%), while two types of R. rugosa were suspected to be at a subspecific level (8.47%).

Keywords

References

  1. Brown WM (1985) The nitochondrial genme of animals. In: Maclntyre RJ(ed), Molecular Evolutionary Genetics, Plenum Press, New York, pp 95-130
  2. Brown WM, George M, Jr. and Wilson AC (1979) Rapid evolution of animal mitochodrial DNA. Proc Natl Acad Sci USA 76: 1967-1971 https://doi.org/10.1073/pnas.76.4.1967
  3. Edwards SV, Arctander P, and Wilson AC (1991) Mitochondrial resolution of adeep branch in the genealogical tree for perching birds. Proc Roy Soc Lond B Biol Sci 243: 99-107 https://doi.org/10.1098/rspb.1991.0017
  4. Felsenstein J (1985) Confidence Limits on phylogenetics: an analysis using the bootstrap. Evolution 39: 789-791
  5. Howell N (1989) Evolutionary conservation of protein regions in redox catalysis. J Mol Evol 29: 157-169 https://doi.org/10.1007/BF02100114
  6. Ilya R and Linda RM (1996) Phylogenetic relationships among bufonoid frogs (Anura: Neobatrachia) inferred from mitochondrial DNA sequences. Mol Phylogenet Evol 5: 533-547 https://doi.org/10.1006/mpev.1996.0048
  7. Irwin DM, Kocher TD, and Wilson AC (1991) Evolution of the cytochrome b gene of mammals. J Mol Evol 32: 128-144 https://doi.org/10.1007/BF02515385
  8. Kim CH (1988) Genetic Variation and Evolution of Korean Amphibians. Ph. D. Dissertation, Inha University, pp 1-132
  9. Kim YR, Yang DE, Lee H, Lee JE, Lee HI, Yang SY, and Lee HY (1999) Genetic differentiation in the mitochondrial cytochrome b gene of Korean brown frogs Rana dybowskii (Amphibia: Ranide). Korean J Biol Sci 3: 199-205
  10. Kocher TD, Thomas WK, Meyer A, Edwards SV, Paavo S, Villablanca FX, and Wilson AC (1989) Dynamics of Mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci USA 86: 6196-6200 https://doi.org/10.1073/pnas.86.16.6196
  11. Kornegay JR, Kocher TD, Williams LA, and Wilson AC (1993) Pathways of lysozyme evolution inferred from the sequences of cytochrome b in birds. J Mol Evol 37: 367-379 https://doi.org/10.1007/BF00178867
  12. Kumar S, Tamura K, Nei M (1993) MEGA, Molecular Evolution Genetics Analysis, Version 1.01 Pennsylvania State University, University Park
  13. Lee HY, Oh SJ, Yang DE, Kim YR, and Yang SY (1997) Genetic differentiation between 2 species in the genes Rana of Korean. Korean J Genet 19: 317-324
  14. Lee HY and Park CS (1991) Genetic studies on Korean znurans: On the mitochondrial DNA differentiation in frogs of the genus Rana. Korean J Genet 13: 1-16
  15. Lee HI, Yang DE, Kim YR, Lee H, Lee JE, Yang SY, and Lee HY (1999a) Genetic varition of the mitochondrial cytochrome b sequence in Korean Rana rugosa (Amphibia: Ranidae). Korean J Biol Sci 3: 89-96
  16. Lee JE, Yang DE, Kim YR, Lee H, Lee HI, Yang SY, and Lee HY (1996b) Genetic relationships of Korean treefrogs (Amphibia; Hylidae) based on mitochondrial cytochrome b and 12S rRNA genes. Korean J Biol Sci 3: 303-309
  17. Ma DP, Zharkikh A, Graur D, Vande berg JL, and Li WH (1993) Structure and evolution of opossum, guinea pig, and porcupine cytochrome b gene. J Mol Evol 9: 327-334 https://doi.org/10.1007/BF00182180
  18. Meyer A (1994) Shortcomings of the cytochrome b gene as a molecular marker. Trends Ecol Evol 9: 278-280 https://doi.org/10.1016/0169-5347(94)90028-0
  19. Oh SJ (1997) The Analysis of Sequences of Cytochrome b Gene in 4 Specoes of Korean Rana. MS Thesis, Inha University, pp 1-62
  20. Okada (1928) Korean amphibian. J Chosen Natl Hist Soc 6: 15-46
  21. Park OY, Lee HI, Yang SY, and Lee HY (1999) Analysis of mitochondrial cytochrome b gene in Korean Bombina orientalis. Korean J Genet 21: 193-199
  22. Roe BA, Ma D, Wilson RK, and Wong JFH (1985) The Complete nucleotide sequence of the Xenopus laevis mitochondrial genome. J Biol Chem 260: 9759-9774
  23. Saitou N and Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4: 406-425
  24. Shannon FA (1956) A reptiles and amphibians of Korea. Herpetologica 12: 22-49
  25. Slobodyanyuk AL (1995) The evolutionary relationshils of two suggested by abalysis of mitochondrial DNA. J Mol Evol 40: 392-399 https://doi.org/10.1007/BF00164025
  26. Sneath PHA and Sokal RR (1973) Numerical Taxonomy. Freeman, San Francisco
  27. Suh JH (1999) Molecular Systematics of Korean Amphibia, Ph. D. Thesis, Inha University, pp 1-158
  28. Sumida M, Ogata M, Kaneda H, and Yonekawa H (1998) Evolutionary relationships among Japanese pond frogs inferrred from mitochondrial DNA sequences of cytochrome b and 12S ribosomal RNA genes. Genes Genet Syst 73: 121-131 https://doi.org/10.1266/ggs.73.121
  29. Swofford DL (1993) Users Manual for PAUP Version 3.1: Phylogenetic Analysis Using Parsimony, lllinois Natural History Survey, Caampaign
  30. Tan AM and Wake DB (1995) MtDNA phylogeography of the California newt,Taricha torosa (Caudata, salamandridae). Mol Phylogenet Evol 4: 383-394 https://doi.org/10.1006/mpev.1995.1036
  31. Whitmore DH, Thai TH, and Craft CM (1994) The largemouth bass cytochrome b gene. J Fish Biol 44: 637-645 https://doi.org/10.1111/j.1095-8649.1994.tb01240.x
  32. Yang SY and Yu CH (1978) Check list of Lorean amphibians. Bull Inst Basic Sci Inha Univ, Inchon 5: 81-90