• Journal of Microbiology and Biotechnology
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• v.16 no.10
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• pp.1554-1560
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• 2006

A Gram-positive, aerobic or facultative anaerobic, non motile, endospore-forming bacterial strain, designated Gsoil $114^T$, was isolated from a soil sample of a ginseng field in Pocheon Province (South Korea), and was characterized taxonomically by using a polyphasic approach. It grew well on nutrient agar medium and utilized a limited number of organic substrates as sole carbon sources, including D-xylose and some other carbohydrates, but did not utilize L-amino acids and organic acids. The isolate was positive for oxidase test but negative for catalase, and negative for degradation of macromolecules such as starch, cellulose, xylan, casein, chitin, and DNA. The G+C content of the genomic DNA was 41.8 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major fatty acids were $anteiso-C_{15:0}$ (32.1%), $iso-C_{15:0}$ (30.5%), and $anteiso-C_{17:0}$ (30.2%). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil $114^T$ fell within the radiation of the cluster comprising Bacillus species and joined Bacillus shackletonii LMG $18435^T$ with a bootstrap value of 95%. The highest 16S rRNA gene sequence similarities were found with Bacillus shackletonii LMG $18435^T$ (97.6%), Bacillus acidicola DSM $14745^T$ (96.9%), Bacillus sporothermodurans DSM $10599^T$ (96.5%), and Bacillus oleronius DSM $9356^T$ (96.5%). The phylogenetic distance from any other validly described species within the genus Bacillus was less than 96%. DNA-DNA hybridization experiments showed that the DNA-similarities between strain Gsoil $114^T$ and closest phylogenetic neighbors were less than 39%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil $114^T$ (=KCTC $13944^T$=DSMZ $18134^T$) was classified in the genus Bacillus as the type strain of a novel species, for which the name Bacillus ginsengihumi sp. nov. is proposed.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.5
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• pp.556-563
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• 2007

We carried out a laboratory scale experiment about the characteristics of chemically active species produced in hybrid gas-liquid discharges. The electrode configuration which had high voltage electrode in the gas phase and ground electrode in the liquid was utilized while high voltage electrode has been typically positioned in the liquid in other studies. Our electrode was configured in such a way as to increase the energy efficiency of chemical reactions by creating a higher electrical field strength and a narrower pulse width than the typical electrode configuration. The highest ozone concentration was obtained at 45 kV which was the medium value in operating voltages. The decrease of solution conductivity increased the resistance of liquid phase and the electric field strength through the gas phase, so ozone gene-ration rate was enhanced. The increase of voltage promoted the production rate of hydrogen peroxide by increasing the electric field strength. In a lower voltage, the increase of solution conductivity increased the degradation rate of $H_2O_2$, so the $H_2O_2$ generation rate decreased. On the other hand, the effects of UV radiation, shock waves etc. increased the $H_2O_2$ generation rate as the solution conductivity increased. A higher rate of $H_2O_2$ generation can be achieved by mixing argon to oxygen which generates a stronger and more stable discharges.

• Korean Journal of Remote Sensing
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• v.9 no.2
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• pp.51-69
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• 1993

Precipitable Water(PW) are retrieved over the tropical and subtropical Pacific Ocean from TOVS infrared and microwave channel brightness temperature and OLR observations by means of stepwise linear regression. The retrieved TOVS PW fields generated by PW$_{sfc}$(71.1 % of the variance and 0.62 g cm$^{-2}$ standard error over the surface) and PW$_{700500}$(71.7 % and 0.17 g cm$^{-2}$ over the 700 - 500 hPa layer) revealed more evolving synoptic signals over the tropical and subtropical Pacific Ocean. The PW$_{sfc}$ dose not show significantly the TP feature because of the representation of the lower PW for high-level clouds not associated with deep convection. There exists some elusion to trace the TP on the PW$_{sfc}$ field if any supplementary information does not provide. But ECMWF analysis has a general tendency of drying the subtropics and moistening the ITCZ (InterTropical Convergence Zone) and SPCZ(South Pacific Convergence Zone). However, although ECMWF analysis is fairly successful in capturing mean patterms, it is unsuccessful in following active synoptic signal like a tropical plume. Similarly, SMMR-PW does not represent the TP well which consists of the highand middle-level clouds, but PW$_{sfc}$ shows underestimated moistness of TP and does not depict significant signal of TP. In the PW field derived from microwave observations, the TP can not be recognized well. Furthermore, the signature of PW$_{sfc}$ was different from OLR for the TP, which implies the presence of high- and middle-layer thin clouds, but in a closer agreement for deep and active convection areas which contain thick middle- and lower-layer clouds; though OLR represented the cloudiness in the tropics well. In synoptically active regions, it differed from OLR analysis, primarily bacause of actual differences in water vapor and cloud features. The signature of PW$_{sfc}$ was different from OLR for the TP.

• The Journal of the Acoustical Society of Korea
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• v.38 no.5
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• pp.485-493
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• 2019

The parametric array phenomenon refers to the generation of a high directivity low frequency wave from a small size radiation plate using the nonlinearity of the medium. In order to improve the usability of parametric array, the beam steering method of low frequency wave is researched, and the beam steering phenomenon is predicted easily using the PD (product directivity) model. However, the PD model can only be applied to Gaussian sources under quasi-linear conditions. Also, the prediction accuracy of low frequency wave beam width is poor. In this paper, a method for predicting the beam steering characteristics of a parametric array that can overcome the limitation of the PD model is investigated. For this purpose, the numerical analysis algorithm of the KZK (Khokhlov-Zabolotskaya-Kuzentsov) equation widely used for parametric array phenomenon prediction is improved. Thus, the beam steering characteristics are calculated by applying the electrical beam steering condition and comparing experimental results. As a result, the numerical analysis using the modified KZK equation algorithm in this study confirms that the beam steering phenomenon can be predicted even in a parametric array source that does not correspond to the quasi-linear condition.

• The Journal of the Acoustical Society of Korea
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• v.40 no.5
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• pp.408-416
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• 2021

In the ultrasonic dispersion, in order to avoid direct contact of the radiation surface of ultrasonic transducers with a liquid sample, the liquid sample is separated by a glass container and it receives ultrasonic energy through an acoustic medium. The transmission efficiency of the ultrasonic energy in the multi-layered ultrasonic system is an important factor. In this study, we suggested a method that can improve the ultrasonic energy transfer efficiency by using a propylene glycol solution as a liquid matching layer in the multi-layered acoustic system. In this method, a propylene glycol solution was filled between the Langevin-type ultrasonic transducer and the luminol solution and the sonoluminescence phenomena in the luminol solution, which is caused by nonlinear effect of high power ultrasound radiated from the transducer, was examined by using a Photo Multiplier Tube (PMT). The transmission efficiency depending on the concentration of propylene glycol solution was observed, and we can see that as the concentration of the propylene glycol solution increased, the matching effect increased while the acoustic attenuation increased. It was confirmed that there is an optimal concentration compromised these two conflicting conditions, and the optimum concentration of the propylene glycol solution was determined experimentally.

• The Journal of the Acoustical Society of Korea
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• v.40 no.4
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• pp.320-329
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• 2021

In this paper, an empirical model of air bubble size to be applied to an air masker for reduction of underwater radiation noise is presented. The proposed model improves the divergence problem under the low-speed flow condition of the existing model derived using Rayleigh's jet instability model and simple continuity condition by introducing a jet flow velocity of air. The jet flow velocity of air is estimated using the bubble size where the liquid is quiescent. In a medium without flow, the size of the bubble is estimated by an empirical method where bubble formation regime is divided into a laminar-flow range, a transition range, and a turbulent-flow range based on the Reynolds number of the injected air. The proposed bubble size model is confirmed to be in good agreement with the Computational Fluid Dynamics (CFD) analysis result and the experimental results of the existing literature. Using the acoustic inversion method, the air bubble population is estimated from the insertion loss measured during the air injection experiment of the air- masker model in a large cavitation tunnel. The results of the experiments and the bubble size model are compared in the paper.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.451-455
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• 2009

Lilium lancifolium, which is one of the Korean native lilies, possesses several useful genetic characteristics such as growth vigor, bulbil formation and resistance to Fusarium. Hybridization was performed to insert useful traits of Asiatic hybrid "Dreamland" as the male parent with upward direction and dark brown spotless on the petal into L. lancifolium as the female parent. The rate of pollen germination of L. lancifolium and "Dreamland" were 30% and 60%, respectively. The pollen germination ratio of $F_1$ progenies at flowering date was 25-57%, which is between the parents'pollen germination ratio. The individual flowering time was between 113 days and 131 days after planting. Of 99% of all plants, the leaf shape index was more than five, which means that the leaf shape of $F_1$ plants is more influenced by female than male parent. The flower color was separated into four types. 51.5% of the progenies showed orange and 44.2% showed light orange, respectively. The colors seemed to be highly influenced by female parents. Mixed colors of parents, orange yellow and light yellow orange, were 3.1%, 1.2%, respectively. Flowering directions were segregated by three different ways, upside, side, and downside. Side direction, which is medium type between female and male parent, was 73%. All progenies showed lots of spot on petals except one offspring. The percentage of bulbil formation on leaf axil was 49.4%. Among those, the maximum number of bulbil formation was 25. The mean number of bulbil formation per plant was five.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.34 no.2
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• pp.113-120
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• 2021

The wave-propagation phenomenon in an infinite medium has been used to describe the physics in many fields of engineering and natural science. Analytical or numerical methods have been developed to obtain solutions to problems related to the wave-propagation phenomenon. Energy radiation into infinite regions must be accurately considered for accurate solutions to these problems; hence, various numerical and mechanical models as well as boundary conditions have been developed. This paper proposes a new boundary condition that can be applied to scalar-wave or horizontally-polarized shear-wave (or SH-wave) propagation problems in layered waveguides. A governing equation is obtained for the SH waves by applying finite-element discretization in the vertical direction of the waveguide and subsequently modified to derive the boundary condition for the infinite region of the waveguide. Using the orthogonality of the eigenmodes for the SH waves in a layered waveguide, the new boundary condition is shown to be equivalent to the existing root-finding absorbing boundary condition; further, the accuracy is shown to increase with the degree of the new boundary condition, and its stability can be proven. The accuracy and stability are then demonstrated by applying the proposed boundary condition to wave-propagation problems in layered waveguides.

• Journal of the Korean Applied Science and Technology
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• v.39 no.5
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• pp.644-651
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• 2022

The skin is the largest organ of the human body and protects the inside of the body. Ultraviolet rays cause various inflammatory reactions in the skin, including photoaging and oxidative damage. The purpose of this study is to investigate the protective effect of Saponaria extract by irradiating UVB on fibroblasts. In this study, the effectiveness of Saponaria showing protective activity against UVB-induced cytotoxicity, oxidative cell death, and NO and PGE₂ production was evaluated. HS68 cells were irradiated with UVB(120 mJ/cm²) and treated with Saponaria extract at various concentrations of 100, 200, and 400 ㎍/mL for 24 hours. Intracellular reactive oxygen species (ROS) generated by ultraviolet B were detected using a spectrofluorometer after DCF-DA staining. Lipid peroxidation was also analyzed by measuring the level of 8-isoprostane secreted into the culture medium. As a result, treatment with Saponaria extract effectively inhibited UVB-induced cytotoxicity. Oxidative cell damage was mediated by PGE₂ in UVB-induced HS68 fibroblasts, which was significantly inhibited by Saponaria extract treatment. In addition, it was evaluated that the protective effect of these extracts was mediated by the inhibition of intracellular ROS production and lipid peroxidation in a concentration-dependent manner. These results suggest that Saponaria extract can be used as an anti-aging functional material because it inhibits skin damage mediated by oxidative stress caused by UVB and exhibits a cellular protective effect.

• Journal of Korean Society of Forest Science
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• v.82 no.2
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• pp.152-165
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• 1993

This is the basic study in order to know the amount of transpirational water loss in a Larix leptorepis stand by a heat pulse method. Especially this study has been measured and discussed the diurnal and seasonal trends of heat pulse velocity by changes of radiation, temperature and humidity, differences of heat pulse velocity by direction and depth in stem, differences of heat pulse velocity by dominant, codominant and suppressed trees, diurnal change of heat pulse velocity by change of leaf water potential, sap flow path way in sapwood by dye penetration and amount of daily and annual transpiration in a tree and stand. The results obtained as follows : 1. Relation between heat pulse velocity(V) and sap flow rate(SFR) was established as a equation of SFR=1.37V($r=0.96^{**}$). 2. The sap flow rate presented in the order of dominant, codominant and suppressed tree, respectively. The daily heat pulse velocity was changed by radiation, temperature and vapor pressure deficit. 3. The heat pulse velocity in individual trees did not differ in early morning and in late night, but had some differed from 12 to 16 hours when radiation was relatively high. 4. The heat pulse velocity and leaf water potential showed similar diurnal variation. 5. The seasonal variation of heat pulse velocity was highest in August, but lowest in October and similar value of heat pulse velocity in the other months. 6. The heat pulse velocity in stem by direction was highest in eastern, but lowest in southern and similar velocity in western and northern. 7. The difference of heat pulse velocity in according to depths was highest in 2.0cm depth, medium in 1.0cm depth, and lowest in 3.0cm depth from surface of stem. 8. The sap flow path way in stem showed spiral ascent turning right pattern in five sample trees, especially showed little spiral ascent turning right in lower part than 3m hight above ground, but very speedy in higher than 3m hight. 9. The amount of sap flow(SF) was presented as a equation of SF=1.37AV and especially SF in dominant tree was larger than in codominant or suppressed tree. 10. The amount of daily transpiration was 30.8ton/ha/day and its composition ratio was 83% at day and 17% at night. 11. The amount of stand transpiration per month was largest in August(1,194ton/ha/month), lowest in May (386ton/ha/month). The amount of stand transpiration per year was 3,983ton/ha/year.